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The map label for this gene is yusK

Identifier: 16080335

GI number: 16080335

Start: 3368839

End: 3370014

Strand: Reverse

Name: yusK

Synonym: BSU32830

Alternate gene names: 16080335

Gene position: 3370014-3368839 (Counterclockwise)

Preceding gene: 16080336

Following gene: 16080334

Centisome position: 79.94

GC content: 52.55

Gene sequence:

>1176_bases
ATGAAAGAGGCAGTCATTGTGTCAGGTGCAAGAACACCGGTTGGAAAAGCAAAAAAAGGATCGTTGGCCACCGTTCGCCC
GGATGATTTGGGAGCGATTTGTGTAAAAGAAACGCTGAAACGGGCAGGCGGCTACGAAGGCAATATTGATGACTTGATTA
TCGGCTGTGCAACACCTGAAGCGGAGCAGGGGCTTAATATGGCGAGAAATATCGGTGCGCTCGCGGGCCTGCCGTACACG
GTTCCGGCGATTACAGTTAACCGCTATTGCTCTTCAGGCCTTCAGTCTATTGCGTATGCAGCAGAAAAAATTATGCTGGG
CGCCTACGATACAGCGATCGCCGGCGGAGCAGAATCCATGTCGCAAGTTCCGATGATGGGGCATGTTACCCGTCCGAACC
TTGCTTTGGCAGAAAAGGCACCGGAATATTATATGAGCATGGGGCACACAGCCGAGCAAGTCGCGAAAAAATACGGCGTC
TCCCGTGAGGATCAAGACGCGTTTGCCGTTCGCAGCCATCAAAACGCCGCTAAAGCCCTTGCAGAAGGAAAATTTAAAGA
TGAAATTGTACCCGTCGAAGTGACGGTGACAGAAATCGGAGAAGACCATAAGCCTATGGAAAAACAGTTTGTGTTTTCTC
AGGATGAAGGTGTTCGCCCGCAAACGACTGCAGACATCTTATCGACATTGCGCCCGGCCTTTTCCGTTGACGGGACGGTG
ACTGCGGGCAATTCCTCACAAACGAGTGACGGAGCGGCGGCTGTCATGCTGATGGACCGGGAAAAAGCCGATGCTCTTGG
CCTGGCGCCGCTTGTGAAATTCCGCTCCTTTGCGGTAGGCGGCGTGCCGCCGGAAGTGATGGGAATCGGACCGGTGGAAG
CGATTCCGCGCGCTTTGAAGCTTGCCGGACTTCAGCTGCAGGACATCGGCTTGTTTGAACTGAATGAAGCTTTTGCCTCA
CAGGCCATTCAAGTAATCAGGGAATTGGGAATTGATGAAGAAAAAGTGAATGTCAACGGGGGCGCAATCGCGCTTGGACA
CCCGCTTGGCTGTACGGGCACAAAGCTTACACTCTCACTCATCCATGAAATGAAACGGAGAAACGAGCAATTTGGAGTCG
TGACCATGTGTATCGGCGGCGGAATGGGAGCGGCCGGTGTATTTGAATTATGCTAA

Upstream 100 bases:

>100_bases
AGAGAAGCGTTTTTGAGTTTGTCAGGAGAGGCAAAATCTCAAGCGAGAATGCAGCATATGCTTGTGAAAGGTAAACCTTT
ACGGAATTAGGAGGGAGCTT

Downstream 100 bases:

>100_bases
AGGGGGAAAAACAAATGGCAAAAAAAGCGGCTGACGTACAAAAAGGCGGCGGATTTTTAATCGAGGATGTCACATACGAT
CAAATGTATACACCGGAGGA

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKEAVIVSGARTPVGKAKKGSLATVRPDDLGAICVKETLKRAGGYEGNIDDLIIGCATPEAEQGLNMARNIGALAGLPYT
VPAITVNRYCSSGLQSIAYAAEKIMLGAYDTAIAGGAESMSQVPMMGHVTRPNLALAEKAPEYYMSMGHTAEQVAKKYGV
SREDQDAFAVRSHQNAAKALAEGKFKDEIVPVEVTVTEIGEDHKPMEKQFVFSQDEGVRPQTTADILSTLRPAFSVDGTV
TAGNSSQTSDGAAAVMLMDREKADALGLAPLVKFRSFAVGGVPPEVMGIGPVEAIPRALKLAGLQLQDIGLFELNEAFAS
QAIQVIRELGIDEEKVNVNGGAIALGHPLGCTGTKLTLSLIHEMKRRNEQFGVVTMCIGGGMGAAGVFELC

Sequences:

>Translated_391_residues
MKEAVIVSGARTPVGKAKKGSLATVRPDDLGAICVKETLKRAGGYEGNIDDLIIGCATPEAEQGLNMARNIGALAGLPYT
VPAITVNRYCSSGLQSIAYAAEKIMLGAYDTAIAGGAESMSQVPMMGHVTRPNLALAEKAPEYYMSMGHTAEQVAKKYGV
SREDQDAFAVRSHQNAAKALAEGKFKDEIVPVEVTVTEIGEDHKPMEKQFVFSQDEGVRPQTTADILSTLRPAFSVDGTV
TAGNSSQTSDGAAAVMLMDREKADALGLAPLVKFRSFAVGGVPPEVMGIGPVEAIPRALKLAGLQLQDIGLFELNEAFAS
QAIQVIRELGIDEEKVNVNGGAIALGHPLGCTGTKLTLSLIHEMKRRNEQFGVVTMCIGGGMGAAGVFELC
>Mature_391_residues
MKEAVIVSGARTPVGKAKKGSLATVRPDDLGAICVKETLKRAGGYEGNIDDLIIGCATPEAEQGLNMARNIGALAGLPYT
VPAITVNRYCSSGLQSIAYAAEKIMLGAYDTAIAGGAESMSQVPMMGHVTRPNLALAEKAPEYYMSMGHTAEQVAKKYGV
SREDQDAFAVRSHQNAAKALAEGKFKDEIVPVEVTVTEIGEDHKPMEKQFVFSQDEGVRPQTTADILSTLRPAFSVDGTV
TAGNSSQTSDGAAAVMLMDREKADALGLAPLVKFRSFAVGGVPPEVMGIGPVEAIPRALKLAGLQLQDIGLFELNEAFAS
QAIQVIRELGIDEEKVNVNGGAIALGHPLGCTGTKLTLSLIHEMKRRNEQFGVVTMCIGGGMGAAGVFELC

Specific function: Involved in the degradation of long-chain fatty acids

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI4501853, Length=390, Percent_Identity=47.9487179487179, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI148539872, Length=396, Percent_Identity=40.9090909090909, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI167614485, Length=401, Percent_Identity=37.9052369077307, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI4557237, Length=400, Percent_Identity=35, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI4504327, Length=436, Percent_Identity=32.3394495412844, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI194353979, Length=390, Percent_Identity=32.0512820512821, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI48994986, Length=394, Percent_Identity=47.2081218274112, Blast_Score=338, Evalue=5e-94,
Organism=Escherichia coli, GI1788554, Length=402, Percent_Identity=46.0199004975124, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1787663, Length=416, Percent_Identity=43.2692307692308, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI87082165, Length=403, Percent_Identity=42.4317617866005, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1788683, Length=419, Percent_Identity=35.5608591885441, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI133906874, Length=396, Percent_Identity=40.1515151515151, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17535921, Length=401, Percent_Identity=38.4039900249377, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI25147385, Length=402, Percent_Identity=34.3283582089552, Blast_Score=220, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI17551802, Length=441, Percent_Identity=31.0657596371882, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17535917, Length=393, Percent_Identity=30.0254452926209, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322031, Length=395, Percent_Identity=42.7848101265823, Blast_Score=286, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6325229, Length=399, Percent_Identity=34.5864661654135, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24655093, Length=396, Percent_Identity=39.3939393939394, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI17648125, Length=397, Percent_Identity=39.2947103274559, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24640423, Length=399, Percent_Identity=36.5914786967419, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI17137578, Length=432, Percent_Identity=32.1759259259259, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FADA_BACSU (O32177)

Other databases:

- EMBL:   AL009126
- PIR:   D70021
- RefSeq:   NP_391162.1
- HSSP:   P27796
- ProteinModelPortal:   O32177
- SMR:   O32177
- EnsemblBacteria:   EBBACT00000001145
- GeneID:   936729
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32830
- NMPDR:   fig|224308.1.peg.3288
- GenoList:   BSU32830
- GeneTree:   EBGT00050000001410
- HOGENOM:   HBG370930
- OMA:   RNEQFGV
- PhylomeDB:   O32177
- ProtClustDB:   PRK07661
- BioCyc:   BSUB:BSU32830-MONOMER
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: =2.3.1.16

Molecular weight: Translated: 41124; Mature: 41124

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3

Important sites: ACT_SITE 90-90 ACT_SITE 347-347 ACT_SITE 377-377

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEAVIVSGARTPVGKAKKGSLATVRPDDLGAICVKETLKRAGGYEGNIDDLIIGCATPE
CCCCEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCC
AEQGLNMARNIGALAGLPYTVPAITVNRYCSSGLQSIAYAAEKIMLGAYDTAIAGGAESM
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHH
SQVPMMGHVTRPNLALAEKAPEYYMSMGHTAEQVAKKYGVSREDQDAFAVRSHQNAAKAL
HCCCCCCCCCCCCCHHHHHCHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AEGKFKDEIVPVEVTVTEIGEDHKPMEKQFVFSQDEGVRPQTTADILSTLRPAFSVDGTV
HCCCCCCCEEEEEEEEEECCCCCCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCCCEE
TAGNSSQTSDGAAAVMLMDREKADALGLAPLVKFRSFAVGGVPPEVMGIGPVEAIPRALK
ECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHH
LAGLQLQDIGLFELNEAFASQAIQVIRELGIDEEKVNVNGGAIALGHPLGCTGTKLTLSL
HCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCHHHHHHH
IHEMKRRNEQFGVVTMCIGGGMGAAGVFELC
HHHHHHCCCCCCEEEEEECCCCCCCHHHHCC
>Mature Secondary Structure
MKEAVIVSGARTPVGKAKKGSLATVRPDDLGAICVKETLKRAGGYEGNIDDLIIGCATPE
CCCCEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCC
AEQGLNMARNIGALAGLPYTVPAITVNRYCSSGLQSIAYAAEKIMLGAYDTAIAGGAESM
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHH
SQVPMMGHVTRPNLALAEKAPEYYMSMGHTAEQVAKKYGVSREDQDAFAVRSHQNAAKAL
HCCCCCCCCCCCCCHHHHHCHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AEGKFKDEIVPVEVTVTEIGEDHKPMEKQFVFSQDEGVRPQTTADILSTLRPAFSVDGTV
HCCCCCCCEEEEEEEEEECCCCCCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHCCCCEE
TAGNSSQTSDGAAAVMLMDREKADALGLAPLVKFRSFAVGGVPPEVMGIGPVEAIPRALK
ECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHH
LAGLQLQDIGLFELNEAFASQAIQVIRELGIDEEKVNVNGGAIALGHPLGCTGTKLTLSL
HCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCHHHHHHH
IHEMKRRNEQFGVVTMCIGGGMGAAGVFELC
HHHHHHCCCCCCEEEEEECCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377