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The map label for this gene is fadN

Identifier: 16080336

GI number: 16080336

Start: 3370025

End: 3372472

Strand: Reverse

Name: fadN

Synonym: BSU32840

Alternate gene names: 16080336

Gene position: 3372472-3370025 (Counterclockwise)

Preceding gene: 16080337

Following gene: 16080335

Centisome position: 80.0

GC content: 47.3

Gene sequence:

>2448_bases
ATGAAAGAAGAAACGGGTTTCATTTTTTTTAGTCAAAAAATGAATGACTATTCATTCAATGTTCAAGGGGGATATCTCAT
GCACAAACACATTCGGAAGGCAGCCGTTTTAGGATCGGGGGTAATGGGTTCCGGGATAGCGGCGCATTTGGCTAACATCG
GAATTCCTGTCCTGCTGCTAGATATTGTGCCGAACGATTTGACAAAGGAAGAGGAAAAAAAGGGGCTGACGAAGGACAGT
TCGGAGGTGCGCAGCAGGCTGAGCCGGCAGGCGATGAAAAAACTGCTGAAACAAAAGCCCGCTCCCCTCACATCAGCGAA
AAATACCTCCTATATTACACCTGGCAATCTGGAGGATGATGCAGAAAAACTGAAAGAAGCAGATTGGATTATTGAGGTCG
TTGTCGAAAACCTTGAAGTCAAAAAGAAAATTTTCGCTCTTGTGGATGAACATCGAAAGACTGGAAGCATCGTCTCAAGC
AACACATCCGGCATATCAGTGCAGGAAATGGCTGAAGGCCGGTCGGATGATTTTAAAGCCCATTTTCTTGGAACGCATTT
TTTTAATCCCGCACGATATTTAAAGCTGCTAGAGATCATTCCGATTAAGGAAACGGATCCTGATATTTTAAAGTTTATGA
CAGCTTTCGGAGAAAATGTCCTCGGGAAAGGCGTTGTTACAGCAAAGGACACACCGAACTTTATCGCAAACCGCATCGGG
ACTTACGGGCTTCTCGTGACAGTCCAAGAAATGCTGAAAGGCGGTTATCAGGTCGGGGAGGTTGATTCGATCACAGGACC
GCTGATCGGCAGGCCGAAAAGCGCCACCTTTAGAACGCTTGATGTGGTCGGCCTCGATACATTCGCCCACGTTGCCCGAA
ATGTGTATGACAAAGCTGATGGCGATGAAAAAGAAGTGTTTCGCATTCCAAGCTTCATGAACGATATGCTGGAAAAAGGG
TGGATCGGAAGCAAGGCCGGCCAAGGCTTTTATAAAAAAGAAGGAAAAACGATTTATGAGCTTGACCCAGTGACGCTTAC
TTACGGCGAGCGGACAAAAATGAAATCACCGGCACTTGAAGCGGCAAAACAGGCAAAAGGGACAAAAGCCAAAATGAAAG
CGCTTATTTATTCGGATGACAGGGCCGGCCGTTTGCTGTGGAATATCACTTCTCAAACCCTGCTTTACTCAGCCGAGCTG
CTTGGCGAGATTGCCGATGATATTCATGCGATTGACCAAGCCATGAAGTGGGGATTCGGCTGGGAACTGGGACCTTTTGA
GATGTGGGACGCCATCGGCCTCAAGCAGTCAGCGGAAAAGCTTGAGCAGCTTGGAGCAGATATGCCTGGCTGGATAAAAG
AGATGCTGGACAAAGGAAATGAAACCTTCTACATCAAAGAGAACGGAACGGTATTTTATTATGACCGCGGCGAATACAGA
GCCGTGAAAGAAAACAAAAAACGAATTCATTTACAAGCACTTAAAGAAACAAAAGGCGTGATTGCGAAAAACAGCGGAGC
AAGCTTAATTGACCTTGGAGATGATGTGGCCCTTCTTGAATTTCATTCGAAAAGCAATGCGATCGGCCTCGATATCATTC
AAATGATCCACAAAGGATTAGAGGAAACAGAACGGAATTACAAGGGGCTTGTCATCGGCAATCAAGGGAAAAATTTCTGC
GTCGGCGCGAATCTCGCCATGATCTTAATGGAAGTCCAAGATGACAACTTTTTGGAAGTTGATTTTGTGATCCGCCGTTT
TCAGGAGACGATGATGAAGATCAAATACAGTGCGAAACCGGTCGTCGCCGCGCCATTTGGCATGACGCTCGGCGGAGGAA
CGGAAGCATGCCTGCCGGCGGCGCGCATTCAAGCGGCAAGCGAAGCCTATATGGGACTTGTGGAATCCGGTGTCGGCCTC
ATCCCTGGCGGAGGAGGTAACAAAGAGCTGTACATCAACCATCTTCGCCGCGGCCATGATCCGATGAATGCGGCAATGAA
AACATTTGAAACAATCGCGATGGCGAAAGTGTCCGCTTCCGCTCAAGAGGCTCGTGAGATGAACATCTTAAAAGAAACGG
ATCAGATCAGCGTGAATCAAGACCACCTGTTATACGATGCGAAGCAGCTGGCCGCATCTTTATACGATACCGGCTGGCGG
CCGCCTGTAAAAGAAAAGGTCAAGGTGCCGGGAGAAACCGGTTATGCGGCACTCTTGCTTGGCGCTGAGCAGATGAAGCT
GTCCGGCTATATATCTGAGCATGATTTTAAGATCGCGAAAAAACTCGCTTATGTCATTGCGGGCGGAAAAGTGCCGTTTG
GTACAGAGGTTGATGAAGAGTATTTATTGGAGATCGAAAGAGAAGCGTTTTTGAGTTTGTCAGGAGAGGCAAAATCTCAA
GCGAGAATGCAGCATATGCTTGTGAAAGGTAAACCTTTACGGAATTAG

Upstream 100 bases:

>100_bases
CCATTCCCTTCACCTCCGGGCTTAAGTTGCCCGACTTTTATAGGCTTCATACAAAAAAATAACAAATTAGCTATTGAAGT
CGAACCGGAAATCTTTTAAG

Downstream 100 bases:

>100_bases
GAGGGAGCTTATGAAAGAGGCAGTCATTGTGTCAGGTGCAAGAACACCGGTTGGAAAAGCAAAAAAAGGATCGTTGGCCA
CCGTTCGCCCGGATGATTTG

Product: enoyl-CoA hydratase / 3-hydroxyacyl-CoA dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 815; Mature: 815

Protein sequence:

>815_residues
MKEETGFIFFSQKMNDYSFNVQGGYLMHKHIRKAAVLGSGVMGSGIAAHLANIGIPVLLLDIVPNDLTKEEEKKGLTKDS
SEVRSRLSRQAMKKLLKQKPAPLTSAKNTSYITPGNLEDDAEKLKEADWIIEVVVENLEVKKKIFALVDEHRKTGSIVSS
NTSGISVQEMAEGRSDDFKAHFLGTHFFNPARYLKLLEIIPIKETDPDILKFMTAFGENVLGKGVVTAKDTPNFIANRIG
TYGLLVTVQEMLKGGYQVGEVDSITGPLIGRPKSATFRTLDVVGLDTFAHVARNVYDKADGDEKEVFRIPSFMNDMLEKG
WIGSKAGQGFYKKEGKTIYELDPVTLTYGERTKMKSPALEAAKQAKGTKAKMKALIYSDDRAGRLLWNITSQTLLYSAEL
LGEIADDIHAIDQAMKWGFGWELGPFEMWDAIGLKQSAEKLEQLGADMPGWIKEMLDKGNETFYIKENGTVFYYDRGEYR
AVKENKKRIHLQALKETKGVIAKNSGASLIDLGDDVALLEFHSKSNAIGLDIIQMIHKGLEETERNYKGLVIGNQGKNFC
VGANLAMILMEVQDDNFLEVDFVIRRFQETMMKIKYSAKPVVAAPFGMTLGGGTEACLPAARIQAASEAYMGLVESGVGL
IPGGGGNKELYINHLRRGHDPMNAAMKTFETIAMAKVSASAQEAREMNILKETDQISVNQDHLLYDAKQLAASLYDTGWR
PPVKEKVKVPGETGYAALLLGAEQMKLSGYISEHDFKIAKKLAYVIAGGKVPFGTEVDEEYLLEIEREAFLSLSGEAKSQ
ARMQHMLVKGKPLRN

Sequences:

>Translated_815_residues
MKEETGFIFFSQKMNDYSFNVQGGYLMHKHIRKAAVLGSGVMGSGIAAHLANIGIPVLLLDIVPNDLTKEEEKKGLTKDS
SEVRSRLSRQAMKKLLKQKPAPLTSAKNTSYITPGNLEDDAEKLKEADWIIEVVVENLEVKKKIFALVDEHRKTGSIVSS
NTSGISVQEMAEGRSDDFKAHFLGTHFFNPARYLKLLEIIPIKETDPDILKFMTAFGENVLGKGVVTAKDTPNFIANRIG
TYGLLVTVQEMLKGGYQVGEVDSITGPLIGRPKSATFRTLDVVGLDTFAHVARNVYDKADGDEKEVFRIPSFMNDMLEKG
WIGSKAGQGFYKKEGKTIYELDPVTLTYGERTKMKSPALEAAKQAKGTKAKMKALIYSDDRAGRLLWNITSQTLLYSAEL
LGEIADDIHAIDQAMKWGFGWELGPFEMWDAIGLKQSAEKLEQLGADMPGWIKEMLDKGNETFYIKENGTVFYYDRGEYR
AVKENKKRIHLQALKETKGVIAKNSGASLIDLGDDVALLEFHSKSNAIGLDIIQMIHKGLEETERNYKGLVIGNQGKNFC
VGANLAMILMEVQDDNFLEVDFVIRRFQETMMKIKYSAKPVVAAPFGMTLGGGTEACLPAARIQAASEAYMGLVESGVGL
IPGGGGNKELYINHLRRGHDPMNAAMKTFETIAMAKVSASAQEAREMNILKETDQISVNQDHLLYDAKQLAASLYDTGWR
PPVKEKVKVPGETGYAALLLGAEQMKLSGYISEHDFKIAKKLAYVIAGGKVPFGTEVDEEYLLEIEREAFLSLSGEAKSQ
ARMQHMLVKGKPLRN
>Mature_815_residues
MKEETGFIFFSQKMNDYSFNVQGGYLMHKHIRKAAVLGSGVMGSGIAAHLANIGIPVLLLDIVPNDLTKEEEKKGLTKDS
SEVRSRLSRQAMKKLLKQKPAPLTSAKNTSYITPGNLEDDAEKLKEADWIIEVVVENLEVKKKIFALVDEHRKTGSIVSS
NTSGISVQEMAEGRSDDFKAHFLGTHFFNPARYLKLLEIIPIKETDPDILKFMTAFGENVLGKGVVTAKDTPNFIANRIG
TYGLLVTVQEMLKGGYQVGEVDSITGPLIGRPKSATFRTLDVVGLDTFAHVARNVYDKADGDEKEVFRIPSFMNDMLEKG
WIGSKAGQGFYKKEGKTIYELDPVTLTYGERTKMKSPALEAAKQAKGTKAKMKALIYSDDRAGRLLWNITSQTLLYSAEL
LGEIADDIHAIDQAMKWGFGWELGPFEMWDAIGLKQSAEKLEQLGADMPGWIKEMLDKGNETFYIKENGTVFYYDRGEYR
AVKENKKRIHLQALKETKGVIAKNSGASLIDLGDDVALLEFHSKSNAIGLDIIQMIHKGLEETERNYKGLVIGNQGKNFC
VGANLAMILMEVQDDNFLEVDFVIRRFQETMMKIKYSAKPVVAAPFGMTLGGGTEACLPAARIQAASEAYMGLVESGVGL
IPGGGGNKELYINHLRRGHDPMNAAMKTFETIAMAKVSASAQEAREMNILKETDQISVNQDHLLYDAKQLAASLYDTGWR
PPVKEKVKVPGETGYAALLLGAEQMKLSGYISEHDFKIAKKLAYVIAGGKVPFGTEVDEEYLLEIEREAFLSLSGEAKSQ
ARMQHMLVKGKPLRN

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Mitochondria or Peroxisomes [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI296179429, Length=303, Percent_Identity=29.7029702970297, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI296179427, Length=320, Percent_Identity=28.75, Blast_Score=111, Evalue=3e-24,
Organism=Homo sapiens, GI20127408, Length=427, Percent_Identity=26.6978922716628, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI68989263, Length=483, Percent_Identity=25.2587991718427, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI261878539, Length=483, Percent_Identity=25.2587991718427, Blast_Score=101, Evalue=3e-21,
Organism=Escherichia coli, GI1787661, Length=473, Percent_Identity=25.5813953488372, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790281, Length=305, Percent_Identity=29.1803278688525, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI1788682, Length=296, Percent_Identity=27.3648648648649, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17553560, Length=303, Percent_Identity=30.6930693069307, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17549919, Length=304, Percent_Identity=30.5921052631579, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17558304, Length=409, Percent_Identity=28.8508557457213, Blast_Score=107, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17563036, Length=299, Percent_Identity=29.0969899665552, Blast_Score=107, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=425, Percent_Identity=27.2941176470588, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=425, Percent_Identity=27.2941176470588, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25144276, Length=306, Percent_Identity=28.4313725490196, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71985923, Length=432, Percent_Identity=27.7777777777778, Blast_Score=93, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71985930, Length=419, Percent_Identity=24.582338902148, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24583077, Length=434, Percent_Identity=26.9585253456221, Blast_Score=98, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24583079, Length=434, Percent_Identity=26.9585253456221, Blast_Score=98, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921000, Length=434, Percent_Identity=26.9585253456221, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45549573, Length=263, Percent_Identity=29.277566539924, Blast_Score=82, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =1.1.1.35 [H]

Molecular weight: Translated: 90150; Mature: 90150

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEETGFIFFSQKMNDYSFNVQGGYLMHKHIRKAAVLGSGVMGSGIAAHLANIGIPVLLL
CCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEE
DIVPNDLTKEEEKKGLTKDSSEVRSRLSRQAMKKLLKQKPAPLTSAKNTSYITPGNLEDD
ECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCHH
AEKLKEADWIIEVVVENLEVKKKIFALVDEHRKTGSIVSSNTSGISVQEMAEGRSDDFKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHCCCCCCCEE
HFLGTHFFNPARYLKLLEIIPIKETDPDILKFMTAFGENVLGKGVVTAKDTPNFIANRIG
EEEEHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHCCCCEEECCCCHHHHHHHHC
TYGLLVTVQEMLKGGYQVGEVDSITGPLIGRPKSATFRTLDVVGLDTFAHVARNVYDKAD
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHCCC
GDEKEVFRIPSFMNDMLEKGWIGSKAGQGFYKKEGKTIYELDPVTLTYGERTKMKSPALE
CCHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEEECCEEEECCCCCCCCCHHHH
AAKQAKGTKAKMKALIYSDDRAGRLLWNITSQTLLYSAELLGEIADDIHAIDQAMKWGFG
HHHHCCCCHHHHEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WELGPFEMWDAIGLKQSAEKLEQLGADMPGWIKEMLDKGNETFYIKENGTVFYYDRGEYR
CCCCCHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCEEEEEECCCCH
AVKENKKRIHLQALKETKGVIAKNSGASLIDLGDDVALLEFHSKSNAIGLDIIQMIHKGL
HHHHCCHHHHHHHHHHHCCEEEECCCCCEEECCCCEEEEEECCCCCCCHHHHHHHHHHHH
EETERNYKGLVIGNQGKNFCVGANLAMILMEVQDDNFLEVDFVIRRFQETMMKIKYSAKP
HHHHCCCCEEEEECCCCCEEEECCEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCC
VVAAPFGMTLGGGTEACLPAARIQAASEAYMGLVESGVGLIPGGGGNKELYINHLRRGHD
EEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEHHHHHCCCC
PMNAAMKTFETIAMAKVSASAQEAREMNILKETDQISVNQDHLLYDAKQLAASLYDTGWR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PPVKEKVKVPGETGYAALLLGAEQMKLSGYISEHDFKIAKKLAYVIAGGKVPFGTEVDEE
CCCHHHEECCCCCCCEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHH
YLLEIEREAFLSLSGEAKSQARMQHMLVKGKPLRN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKEETGFIFFSQKMNDYSFNVQGGYLMHKHIRKAAVLGSGVMGSGIAAHLANIGIPVLLL
CCCCCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEE
DIVPNDLTKEEEKKGLTKDSSEVRSRLSRQAMKKLLKQKPAPLTSAKNTSYITPGNLEDD
ECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCHH
AEKLKEADWIIEVVVENLEVKKKIFALVDEHRKTGSIVSSNTSGISVQEMAEGRSDDFKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHCCCCCCCEE
HFLGTHFFNPARYLKLLEIIPIKETDPDILKFMTAFGENVLGKGVVTAKDTPNFIANRIG
EEEEHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHCCCCEEECCCCHHHHHHHHC
TYGLLVTVQEMLKGGYQVGEVDSITGPLIGRPKSATFRTLDVVGLDTFAHVARNVYDKAD
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHCCC
GDEKEVFRIPSFMNDMLEKGWIGSKAGQGFYKKEGKTIYELDPVTLTYGERTKMKSPALE
CCHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEEECCEEEECCCCCCCCCHHHH
AAKQAKGTKAKMKALIYSDDRAGRLLWNITSQTLLYSAELLGEIADDIHAIDQAMKWGFG
HHHHCCCCHHHHEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WELGPFEMWDAIGLKQSAEKLEQLGADMPGWIKEMLDKGNETFYIKENGTVFYYDRGEYR
CCCCCHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCEEEEEECCCCH
AVKENKKRIHLQALKETKGVIAKNSGASLIDLGDDVALLEFHSKSNAIGLDIIQMIHKGL
HHHHCCHHHHHHHHHHHCCEEEECCCCCEEECCCCEEEEEECCCCCCCHHHHHHHHHHHH
EETERNYKGLVIGNQGKNFCVGANLAMILMEVQDDNFLEVDFVIRRFQETMMKIKYSAKP
HHHHCCCCEEEEECCCCCEEEECCEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCC
VVAAPFGMTLGGGTEACLPAARIQAASEAYMGLVESGVGLIPGGGGNKELYINHLRRGHD
EEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEHHHHHCCCC
PMNAAMKTFETIAMAKVSASAQEAREMNILKETDQISVNQDHLLYDAKQLAASLYDTGWR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PPVKEKVKVPGETGYAALLLGAEQMKLSGYISEHDFKIAKKLAYVIAGGKVPFGTEVDEE
CCCHHHEECCCCCCCEEEEECHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHH
YLLEIEREAFLSLSGEAKSQARMQHMLVKGKPLRN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]