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The map label for this gene is yusI

Identifier: 16080333

GI number: 16080333

Start: 3366573

End: 3366929

Strand: Reverse

Name: yusI

Synonym: BSU32810

Alternate gene names: 16080333

Gene position: 3366929-3366573 (Counterclockwise)

Preceding gene: 16080334

Following gene: 16080332

Centisome position: 79.87

GC content: 42.86

Gene sequence:

>357_bases
ATGTCGTTAACTTTTTACTGGTACCCTAAATGCGGAACGTGCCGCAAAGCAAAGAAGTGGCTTGAAGAGCATGGGAAAGA
AATAAACGAAATACATATTGCTGAGCAGCCGCCAAGCAAAGAAGAATTAAAAGCGCTTTATGAAAAAAGCGGGCTGGATC
TCAAGAAATTTTTCAACACGAGCGGCATGAAATACCGCGAGCTCAATCTCAAGGAAAAGCTGTATCATATGTCAGAGGAC
GAACAGCTCGAACTGCTGGCTTCAGATGGTATGCTAATTAAACGCCCGCTTACAACAGACGGCGAAAAAGTGACTGTCGG
ATTTAAAGAAGACCAATTTGAAGAAAACTGGGCTTAA

Upstream 100 bases:

>100_bases
GCTCCGCATGCGCGGAGCTCTTTTTTAAGAAGCACTGTTTTAGGTTTTTAAAACAAACTGTGTTAAAATAACCTCGATTA
TTTTATTGAGGGGGAAGAAG

Downstream 100 bases:

>100_bases
TGTAAATTTTTTTTGAAAAGTGTGGTATCTTCTTATTGTAGTGCTGTAAAAAATGGAGGGATTCAATTGAGCATACCAAA
AGATTTGCGTTACTCAGGAG

Product: oxidoreductase

Products: arsenite; oxidized glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 118; Mature: 117

Protein sequence:

>118_residues
MSLTFYWYPKCGTCRKAKKWLEEHGKEINEIHIAEQPPSKEELKALYEKSGLDLKKFFNTSGMKYRELNLKEKLYHMSED
EQLELLASDGMLIKRPLTTDGEKVTVGFKEDQFEENWA

Sequences:

>Translated_118_residues
MSLTFYWYPKCGTCRKAKKWLEEHGKEINEIHIAEQPPSKEELKALYEKSGLDLKKFFNTSGMKYRELNLKEKLYHMSED
EQLELLASDGMLIKRPLTTDGEKVTVGFKEDQFEENWA
>Mature_117_residues
SLTFYWYPKCGTCRKAKKWLEEHGKEINEIHIAEQPPSKEELKALYEKSGLDLKKFFNTSGMKYRELNLKEKLYHMSEDE
QLELLASDGMLIKRPLTTDGEKVTVGFKEDQFEENWA

Specific function: Reduction Of Arsenate [As(V)] To Arsenite [As(III)]. This Protein Expands The Substrate Specificity Of Arsab Pump Which Can Extrude Arsenite And Antimonite To Allow For Arsenate Pumping And Resistance (By Similarity). [C]

COG id: COG1393

COG function: function code P; Arsenate reductase and related proteins, glutaredoxin family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsC family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YUSI_BACSU (O32175)

Other databases:

- EMBL:   AL009126
- PIR:   B70021
- RefSeq:   NP_391160.1
- ProteinModelPortal:   O32175
- SMR:   O32175
- EnsemblBacteria:   EBBACT00000003501
- GeneID:   936722
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU32810
- NMPDR:   fig|224308.1.peg.3286
- GenoList:   BSU32810
- GeneTree:   EBGT00050000001358
- HOGENOM:   HBG734370
- OMA:   KCGTCRK
- PhylomeDB:   O32175
- ProtClustDB:   CLSK887845
- BioCyc:   BSUB:BSU32810-MONOMER
- InterPro:   IPR006660
- InterPro:   IPR012336
- InterPro:   IPR012335
- InterPro:   IPR006504
- Gene3D:   G3DSA:3.40.30.10
- TIGRFAMs:   TIGR01617

Pfam domain/function: PF03960 ArsC; SSF52833 Thiordxn-like_fd

EC number: 1.20.4.1 [C]

Molecular weight: Translated: 13909; Mature: 13778

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS51353 ARSC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTFYWYPKCGTCRKAKKWLEEHGKEINEIHIAEQPPSKEELKALYEKSGLDLKKFFNT
CCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHCC
SGMKYRELNLKEKLYHMSEDEQLELLASDGMLIKRPLTTDGEKVTVGFKEDQFEENWA
CCCEEEECCHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SLTFYWYPKCGTCRKAKKWLEEHGKEINEIHIAEQPPSKEELKALYEKSGLDLKKFFNT
CEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHCC
SGMKYRELNLKEKLYHMSEDEQLELLASDGMLIKRPLTTDGEKVTVGFKEDQFEENWA
CCCEEEECCHHHHHHCCCCCCCEEEEECCCEEEECCCCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: arsenate; reduced glutathione [C]

Specific reaction: arsenate + reduced glutathione = arsenite + oxidized glutathione [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377