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The map label for this gene is yusH

Identifier: 16080332

GI number: 16080332

Start: 3366123

End: 3366506

Strand: Reverse

Name: yusH

Synonym: BSU32800

Alternate gene names: 16080332

Gene position: 3366506-3366123 (Counterclockwise)

Preceding gene: 16080333

Following gene: 16080331

Centisome position: 79.86

GC content: 45.05

Gene sequence:

>384_bases
TTGAGCATACCAAAAGATTTGCGTTACTCAGGAGAACACGAGTGGGTAAAAGTTGAAGGAGAAAAAGCCAGAATCGGAAT
TACGCATTTTGCCCAGTCCGAACTAGGCGACATCGTGTTTGTCGAACTTCCTGAAGTGGGCGCTGAAATCAAAGCGGACG
AGCCATTCGGCAGTGTTGAATCCGTTAAAACGGTATCTGAGCTTTATGCACCGATTAACGGAACGGTTGTGGAAGTAAAC
GAAGATCTTGATGACAGTCCGGAATTCGTCAACGAATCTCCGTACGAAAAAGCATGGATGATCGTCGTTGAGCCATCTGA
CGCTTCTGAAATTGAAAAACTGATGACAGCAGAGCAATACGAAGAGATGACACAAGAAGACTAA

Upstream 100 bases:

>100_bases
TAAAGAAGACCAATTTGAAGAAAACTGGGCTTAATGTAAATTTTTTTTGAAAAGTGTGGTATCTTCTTATTGTAGTGCTG
TAAAAAATGGAGGGATTCAA

Downstream 100 bases:

>100_bases
AACAGGATAGCCGTAAAAGCAATTGGACACGCTAACGTTATGGGAGGTGTCCAATATGGCTTTCGACAAACAAAAGCTCG
ATGTCACCAATGACGTGACA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MSIPKDLRYSGEHEWVKVEGEKARIGITHFAQSELGDIVFVELPEVGAEIKADEPFGSVESVKTVSELYAPINGTVVEVN
EDLDDSPEFVNESPYEKAWMIVVEPSDASEIEKLMTAEQYEEMTQED

Sequences:

>Translated_127_residues
MSIPKDLRYSGEHEWVKVEGEKARIGITHFAQSELGDIVFVELPEVGAEIKADEPFGSVESVKTVSELYAPINGTVVEVN
EDLDDSPEFVNESPYEKAWMIVVEPSDASEIEKLMTAEQYEEMTQED
>Mature_126_residues
SIPKDLRYSGEHEWVKVEGEKARIGITHFAQSELGDIVFVELPEVGAEIKADEPFGSVESVKTVSELYAPINGTVVEVNE
DLDDSPEFVNESPYEKAWMIVVEPSDASEIEKLMTAEQYEEMTQED

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=115, Percent_Identity=45.2173913043478, Blast_Score=116, Evalue=6e-27,
Organism=Homo sapiens, GI89057342, Length=115, Percent_Identity=44.3478260869565, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=45.6692913385827, Blast_Score=122, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17507493, Length=119, Percent_Identity=44.5378151260504, Blast_Score=115, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17551294, Length=119, Percent_Identity=42.8571428571429, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=44.53125, Blast_Score=114, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=46.6666666666667, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14254; Mature: 14123

Theoretical pI: Translated: 3.81; Mature: 3.81

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPKDLRYSGEHEWVKVEGEKARIGITHFAQSELGDIVFVELPEVGAEIKADEPFGSVE
CCCCCCCCCCCCCCEEEEECCEEEEEHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHH
SVKTVSELYAPINGTVVEVNEDLDDSPEFVNESPYEKAWMIVVEPSDASEIEKLMTAEQY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
EEMTQED
HHHHCCC
>Mature Secondary Structure 
SIPKDLRYSGEHEWVKVEGEKARIGITHFAQSELGDIVFVELPEVGAEIKADEPFGSVE
CCCCCCCCCCCCCEEEEECCEEEEEHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCHH
SVKTVSELYAPINGTVVEVNEDLDDSPEFVNESPYEKAWMIVVEPSDASEIEKLMTAEQY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
EEMTQED
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA