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The map label for this gene is yugJ

Identifier: 16080189

GI number: 16080189

Start: 3223471

End: 3224634

Strand: Reverse

Name: yugJ

Synonym: BSU31370

Alternate gene names: 16080189

Gene position: 3224634-3223471 (Counterclockwise)

Preceding gene: 16080191

Following gene: 16080188

Centisome position: 76.49

GC content: 45.53

Gene sequence:

>1164_bases
ATGCAAAACTTTACATACTGGAATCCGACCAAATTAATTTTCGGGCGCGGCGAAGTGGAAAGACTTCCGGAGGAACTCAA
ACCTTACGGAAAAAACGTATTGCTTGTGTACGGAGGCGGCAGCATTAAACGCAGCGGCCTGTATGATCAAGTGATTGAAC
AGCTGAATAAAGCCGGAGCGACCGTGCATGAATTAGCAGGTGTGGAACCGAATCCTCGTGTGTCGACTGTTAATAAAGGT
GTTGCCATCTGTAAAGAACAAAACATTGATTTCTTGCTGGCTGTCGGAGGCGGAAGCGTAATCGACTGTACAAAAGCGAT
TGCCGCAGGAGCGAAGTATGATGGTGATGCGTGGGATATCGTTACGAAAAAGCATCAGCCAAAAGATGCTTTGCCATTCG
GAACGGTATTGACTCTCGCTGCAACTGGTTCAGAAATGAACTCAGGATCTGTTATTACAAACTGGGAAACAAAAGAAAAA
TACGGCTGGGGCAGCCCGCTCGTATTCCCTAAATTCTCGATTCTTGATCCGGTGAATACATTCACCGTACCTAAAAACCA
CACGATCTACGGGATGGTTGACATGATGAGCCACGTATTCGAACAATACTTCCATCATGTATCAAACACGCCGTATCAGG
ACCGCATGTGTGAATCACTTTTGCGTACAGTCATTGAAACAGCGCCTAAGCTGATCAATGATCTCGAAAATTACGAATTG
CGTGAGACGATCCTGTACACAGGAACAATCGCATTAAACGGCATGCTTTCTATGGGGGCAAGAGGGGATTGGGCTACTCA
TAATATTGAACATGCAGTATCAGCCGTTTATGATATTCCGCATGCCGGCGGACTGGCGATTCTGTTCCCGAATTGGATGA
GACACACATTGTCTGAAAACCCTGCCCGCATGAAACAGCTTGCAGTTCGCGTGTTTGATGTTGAAGAAGCAGGTAAAACG
GATGAAGAAATTGCCCTTGAAGGTATCGATAAGCTGTCCGCATTCTGGACAAGTCTTGGCGCTCCGAACCGTCTTGCTGA
TTATGATATTAATGATGAGCAGCTTGACACAATCGCTGACAAGGCAATGGCTAACGGTACATTCGGCCAATTTAAATCAC
TCAACAAAGAAGATGTGCTGTCAATTTTGAAAGCATCACTATAA

Upstream 100 bases:

>100_bases
TTGCAAAATAAAACCATCTGGTATGCTGATCGGTGAGAGCTGATTGCTTTACATGTGGTGAGGACATTCTGCATAATAAA
AAATCTAAGGAGGAGATGTC

Downstream 100 bases:

>100_bases
AAAAAGGGGAAATAGCCGTTTGGCTGCTTCCCTTTTTCTTTTTTGTCAACTTATGTTACAATGGAAAATCTGTTTTTTAC
CAGCATCATTTGGAGGTTTT

Product: NADH-dependent butanol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MQNFTYWNPTKLIFGRGEVERLPEELKPYGKNVLLVYGGGSIKRSGLYDQVIEQLNKAGATVHELAGVEPNPRVSTVNKG
VAICKEQNIDFLLAVGGGSVIDCTKAIAAGAKYDGDAWDIVTKKHQPKDALPFGTVLTLAATGSEMNSGSVITNWETKEK
YGWGSPLVFPKFSILDPVNTFTVPKNHTIYGMVDMMSHVFEQYFHHVSNTPYQDRMCESLLRTVIETAPKLINDLENYEL
RETILYTGTIALNGMLSMGARGDWATHNIEHAVSAVYDIPHAGGLAILFPNWMRHTLSENPARMKQLAVRVFDVEEAGKT
DEEIALEGIDKLSAFWTSLGAPNRLADYDINDEQLDTIADKAMANGTFGQFKSLNKEDVLSILKASL

Sequences:

>Translated_387_residues
MQNFTYWNPTKLIFGRGEVERLPEELKPYGKNVLLVYGGGSIKRSGLYDQVIEQLNKAGATVHELAGVEPNPRVSTVNKG
VAICKEQNIDFLLAVGGGSVIDCTKAIAAGAKYDGDAWDIVTKKHQPKDALPFGTVLTLAATGSEMNSGSVITNWETKEK
YGWGSPLVFPKFSILDPVNTFTVPKNHTIYGMVDMMSHVFEQYFHHVSNTPYQDRMCESLLRTVIETAPKLINDLENYEL
RETILYTGTIALNGMLSMGARGDWATHNIEHAVSAVYDIPHAGGLAILFPNWMRHTLSENPARMKQLAVRVFDVEEAGKT
DEEIALEGIDKLSAFWTSLGAPNRLADYDINDEQLDTIADKAMANGTFGQFKSLNKEDVLSILKASL
>Mature_387_residues
MQNFTYWNPTKLIFGRGEVERLPEELKPYGKNVLLVYGGGSIKRSGLYDQVIEQLNKAGATVHELAGVEPNPRVSTVNKG
VAICKEQNIDFLLAVGGGSVIDCTKAIAAGAKYDGDAWDIVTKKHQPKDALPFGTVLTLAATGSEMNSGSVITNWETKEK
YGWGSPLVFPKFSILDPVNTFTVPKNHTIYGMVDMMSHVFEQYFHHVSNTPYQDRMCESLLRTVIETAPKLINDLENYEL
RETILYTGTIALNGMLSMGARGDWATHNIEHAVSAVYDIPHAGGLAILFPNWMRHTLSENPARMKQLAVRVFDVEEAGKT
DEEIALEGIDKLSAFWTSLGAPNRLADYDINDEQLDTIADKAMANGTFGQFKSLNKEDVLSILKASL

Specific function: Unknown

COG id: COG1979

COG function: function code C; Uncharacterized oxidoreductases, Fe-dependent alcohol dehydrogenase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789386, Length=369, Percent_Identity=40.1084010840108, Blast_Score=267, Evalue=8e-73,
Organism=Escherichia coli, GI48994951, Length=388, Percent_Identity=25.2577319587629, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789163, Length=389, Percent_Identity=24.6786632390745, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082107, Length=343, Percent_Identity=24.198250728863, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787493, Length=333, Percent_Identity=25.5255255255255, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17537053, Length=418, Percent_Identity=25.1196172248804, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321181, Length=382, Percent_Identity=26.1780104712042, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YUGJ_BACSU (O05239)

Other databases:

- EMBL:   Z93934
- EMBL:   AL009126
- PIR:   A70011
- RefSeq:   NP_391015.1
- ProteinModelPortal:   O05239
- SMR:   O05239
- EnsemblBacteria:   EBBACT00000001100
- GeneID:   937164
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31370
- NMPDR:   fig|224308.1.peg.3140
- GenoList:   BSU31370
- GeneTree:   EBGT00050000000021
- HOGENOM:   HBG742415
- OMA:   ITNWETK
- PhylomeDB:   O05239
- ProtClustDB:   CLSK2460952
- BioCyc:   BSUB:BSU31370-MONOMER
- InterPro:   IPR001670
- InterPro:   IPR018211

Pfam domain/function: PF00465 Fe-ADH

EC number: 1.1.-.- [C]

Molecular weight: Translated: 42733; Mature: 42733

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00913 ADH_IRON_1; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNFTYWNPTKLIFGRGEVERLPEELKPYGKNVLLVYGGGSIKRSGLYDQVIEQLNKAGA
CCCCCEECCCEEEECCCHHHHCHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCC
TVHELAGVEPNPRVSTVNKGVAICKEQNIDFLLAVGGGSVIDCTKAIAAGAKYDGDAWDI
HHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCCCEEE
VTKKHQPKDALPFGTVLTLAATGSEMNSGSVITNWETKEKYGWGSPLVFPKFSILDPVNT
ECCCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCCHHCCCCCCCCCCCCHHHCCCCCC
FTVPKNHTIYGMVDMMSHVFEQYFHHVSNTPYQDRMCESLLRTVIETAPKLINDLENYEL
EECCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
RETILYTGTIALNGMLSMGARGDWATHNIEHAVSAVYDIPHAGGLAILFPNWMRHTLSEN
HHHHEEECHHHEHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHCCC
PARMKQLAVRVFDVEEAGKTDEEIALEGIDKLSAFWTSLGAPNRLADYDINDEQLDTIAD
HHHHHHHHHHHEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
KAMANGTFGQFKSLNKEDVLSILKASL
HHHCCCCCHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQNFTYWNPTKLIFGRGEVERLPEELKPYGKNVLLVYGGGSIKRSGLYDQVIEQLNKAGA
CCCCCEECCCEEEECCCHHHHCHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCC
TVHELAGVEPNPRVSTVNKGVAICKEQNIDFLLAVGGGSVIDCTKAIAAGAKYDGDAWDI
HHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCCCEEE
VTKKHQPKDALPFGTVLTLAATGSEMNSGSVITNWETKEKYGWGSPLVFPKFSILDPVNT
ECCCCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCCHHCCCCCCCCCCCCHHHCCCCCC
FTVPKNHTIYGMVDMMSHVFEQYFHHVSNTPYQDRMCESLLRTVIETAPKLINDLENYEL
EECCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
RETILYTGTIALNGMLSMGARGDWATHNIEHAVSAVYDIPHAGGLAILFPNWMRHTLSEN
HHHHEEECHHHEHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHCCC
PARMKQLAVRVFDVEEAGKTDEEIALEGIDKLSAFWTSLGAPNRLADYDINDEQLDTIAD
HHHHHHHHHHHEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
KAMANGTFGQFKSLNKEDVLSILKASL
HHHCCCCCHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377