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The map label for this gene is yugI

Identifier: 16080191

GI number: 16080191

Start: 3225178

End: 3225570

Strand: Reverse

Name: yugI

Synonym: BSU31390

Alternate gene names: 16080191

Gene position: 3225570-3225178 (Counterclockwise)

Preceding gene: 50812284

Following gene: 16080189

Centisome position: 76.51

GC content: 39.69

Gene sequence:

>393_bases
ATGGCAGCAAAATTTGAAGTGGGCAGTGTTTACACTGGTAAAGTTACAGGATTACAAGCGTATGGTGCGTTTGTTGCATT
AGATGAAGAAACTCAAGGTTTAGTACACATTTCTGAAGTGACTCACGGTTTCGTAAAAGATATCAACGAGCACCTTTCAG
TTGGCGACGAAGTACAAGTAAAAGTACTTGCTGTTGATGAAGAAAAAGGCAAAATCAGCCTTTCAATTCGTGCGACACAA
GCTGCGCCTGAGAAAAAAGAAAGCAAACCAAGAAAACCTAAAGCTGCTCAAGTAAGCGAAGAAGCTTCTACACCACAAGG
TTTCAACACATTAAAAGATAAGCTTGAAGAGTGGATCGAAATGTCCAACCGCAAAGACCTTATCAAAAAATAA

Upstream 100 bases:

>100_bases
TTTTTTATAAAAAAGTTTTCTTTGCGGAAATAAGACATGTGTGATATAATTTTAAATTGCGTATAAACAGATATTTAATT
TAACTTAGGAGTTGTTATGA

Downstream 100 bases:

>100_bases
GCATGAAAAAAGCACCGGACAGGAATGTTCGGTGCTTTTGATTTCATGCAAAATCCTATTTCAGCGTGATTGATATCCTA
TCGCATTTCAGGATTTGCTG

Product: general stress protein 13

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: GSP13

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MAAKFEVGSVYTGKVTGLQAYGAFVALDEETQGLVHISEVTHGFVKDINEHLSVGDEVQVKVLAVDEEKGKISLSIRATQ
AAPEKKESKPRKPKAAQVSEEASTPQGFNTLKDKLEEWIEMSNRKDLIKK

Sequences:

>Translated_130_residues
MAAKFEVGSVYTGKVTGLQAYGAFVALDEETQGLVHISEVTHGFVKDINEHLSVGDEVQVKVLAVDEEKGKISLSIRATQ
AAPEKKESKPRKPKAAQVSEEASTPQGFNTLKDKLEEWIEMSNRKDLIKK
>Mature_129_residues
AAKFEVGSVYTGKVTGLQAYGAFVALDEETQGLVHISEVTHGFVKDINEHLSVGDEVQVKVLAVDEEKGKISLSIRATQA
APEKKESKPRKPKAAQVSEEASTPQGFNTLKDKLEEWIEMSNRKDLIKK

Specific function: Involved In Mrna Degradation. Hydrolyzes Single-Stranded Polyribonucleotides Processively In The 3' To 5' Direction. Involved In The RNA Degradosome, A Multi-Enzyme Complex Important In RNA Processing And Messenger RNA Degradation. [C]

COG id: COG1098

COG function: function code J; Predicted RNA binding protein (contains ribosomal protein S1 domain)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Escherichia coli, GI145693187, Length=77, Percent_Identity=49.3506493506493, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI87082262, Length=103, Percent_Identity=33.0097087378641, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1787140, Length=78, Percent_Identity=44.8717948717949, Blast_Score=59, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17535281, Length=110, Percent_Identity=34.5454545454545, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): GS13_BACSU (P80870)

Other databases:

- EMBL:   Z93934
- EMBL:   AL009126
- PIR:   H70010
- RefSeq:   NP_391017.1
- PDB:   2K4K
- PDBsum:   2K4K
- ProteinModelPortal:   P80870
- SMR:   P80870
- EnsemblBacteria:   EBBACT00000000199
- GeneID:   938849
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31390
- NMPDR:   fig|224308.1.peg.3142
- GenoList:   BSU31390
- GeneTree:   EBGT00050000001823
- HOGENOM:   HBG714909
- OMA:   DINEHLS
- ProtClustDB:   PRK08059
- BioCyc:   BSUB:BSU31390-MONOMER
- GO:   GO:0005840
- GO:   GO:0006412
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR000110
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- PRINTS:   PR00681
- SMART:   SM00316

Pfam domain/function: PF00575 S1; SSF50249 Nucleic_acid_OB

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 14283; Mature: 14152

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKFEVGSVYTGKVTGLQAYGAFVALDEETQGLVHISEVTHGFVKDINEHLSVGDEVQV
CCCCCCCCCEEECCCCCEEECCEEEEECCCCCCEEEHHHHHHHHHHHHHHHCCCCCCEEE
KVLAVDEEKGKISLSIRATQAAPEKKESKPRKPKAAQVSEEASTPQGFNTLKDKLEEWIE
EEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
MSNRKDLIKK
HCCCHHHHCC
>Mature Secondary Structure 
AAKFEVGSVYTGKVTGLQAYGAFVALDEETQGLVHISEVTHGFVKDINEHLSVGDEVQV
CCCCCCCCEEECCCCCEEECCEEEEECCCCCCEEEHHHHHHHHHHHHHHHCCCCCCEEE
KVLAVDEEKGKISLSIRATQAAPEKKESKPRKPKAAQVSEEASTPQGFNTLKDKLEEWIE
EEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
MSNRKDLIKK
HCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 9298659