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The map label for this gene is yugK

Identifier: 16080188

GI number: 16080188

Start: 3222195

End: 3223367

Strand: Reverse

Name: yugK

Synonym: BSU31360

Alternate gene names: 16080188

Gene position: 3223367-3222195 (Counterclockwise)

Preceding gene: 16080189

Following gene: 255767713

Centisome position: 76.46

GC content: 44.25

Gene sequence:

>1173_bases
ATGGAAAATTTCACTTATTATAATCCGACAAAGCTGATTTTTGGAAAAGGTCAGCTTGAACAATTAAGAAAAGAATTCAA
ACGATACGGCAAGAATGTACTGCTTGTTTACGGGGGCGGCAGCATTAAACGCAACGGCCTTTATGATCAAGTCACAGGCA
TTTTAAAAGAAGAGGGCGCTGTTGTTCATGAGCTGTCAGGTGTAGAGCCAAACCCGCGTCTTGCGACAGTGGAAAAAGGC
ATAGGACTTTGCAGAGAGCATGACATTGATTTTCTGCTTGCTGTCGGCGGAGGCAGTGTGATTGACTGTACAAAGGCAAT
CGCAGCTGGCGTCAAATATGACGGTGACGCTTGGGATATTTTCAGCAAAAAAGTAACAGCGGAGGATGCGCTGCCGTTTG
GCACTGTTTTAACTCTTGCTGCAACAGGGTCTGAAATGAACCCTGATTCCGTGATTACAAACTGGGAAACAAACGAGAAA
TTTGTATGGGGCAGCAATGTCACTCATCCGCGTTTCTCTATTTTAGACCCTGAAAATACGTTCACCGTTCCAGAAAATCA
AACAGTATACGGCATGGTTGACATGATGAGCCACGTATTCGAACAATACTTCCATAATGTTGAAAACACGCCGCTTCAGG
ATAGAATGTGCTTTGCTGTTTTGCAGACGGTCATCGAAACAGCTCCTAAGCTTCTTGAAGATCTGGAAAACTACGAACTT
CGTGAAACGATTTTGTATGCTGGTACTATTGCTTTAAACGGCACGCTCCAAATGGGATACTTCGGTGACTGGGCTTCTCA
TACAATGGAACACGCTGTTTCAGCTGTATATGATATTCCGCACGCGGGCGGTTTGGCAATACTGTTCCCAAATTGGATGA
GATACACGCTTGATACAAATGTAGGCCGTTTTAAAAACCTTATGCTCAACATGTTTGACATTGATACTGAAGGCAAAACA
GATAAAGAAATTGCGCTTGAAGGAATCGATAAACTGTCTGCGTTCTGGACAAGCCTCGGTGCACCTTCTCGTCTTGCTGA
TTACAATATTGGAGAAGAAAAGCTTGAGCTGATTGCTGATATCGCAGCCAAGGAAATGGAACACGGCGGCTTCGGCAACT
TCCAAAAACTGAACAAAGATGACGTGCTTGCCATCCTTCGCGCGTCTCTATAA

Upstream 100 bases:

>100_bases
AAGGGGAAATAGCCGTTTGGCTGCTTCCCTTTTTCTTTTTTGTCAACTTATGTTACAATGGAAAATCTGTTTTTTACCAG
CATCATTTGGAGGTTTTACT

Downstream 100 bases:

>100_bases
ACCCAAAGGGCGGAATCCAGTCATTCCGCCCTTTCTTCTTGACTTGATTTCACAGATAAGTTCATATAAAGTGAAAGATG
AAAACATTATACCGTTATGG

Product: NADH-dependent butanol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MENFTYYNPTKLIFGKGQLEQLRKEFKRYGKNVLLVYGGGSIKRNGLYDQVTGILKEEGAVVHELSGVEPNPRLATVEKG
IGLCREHDIDFLLAVGGGSVIDCTKAIAAGVKYDGDAWDIFSKKVTAEDALPFGTVLTLAATGSEMNPDSVITNWETNEK
FVWGSNVTHPRFSILDPENTFTVPENQTVYGMVDMMSHVFEQYFHNVENTPLQDRMCFAVLQTVIETAPKLLEDLENYEL
RETILYAGTIALNGTLQMGYFGDWASHTMEHAVSAVYDIPHAGGLAILFPNWMRYTLDTNVGRFKNLMLNMFDIDTEGKT
DKEIALEGIDKLSAFWTSLGAPSRLADYNIGEEKLELIADIAAKEMEHGGFGNFQKLNKDDVLAILRASL

Sequences:

>Translated_390_residues
MENFTYYNPTKLIFGKGQLEQLRKEFKRYGKNVLLVYGGGSIKRNGLYDQVTGILKEEGAVVHELSGVEPNPRLATVEKG
IGLCREHDIDFLLAVGGGSVIDCTKAIAAGVKYDGDAWDIFSKKVTAEDALPFGTVLTLAATGSEMNPDSVITNWETNEK
FVWGSNVTHPRFSILDPENTFTVPENQTVYGMVDMMSHVFEQYFHNVENTPLQDRMCFAVLQTVIETAPKLLEDLENYEL
RETILYAGTIALNGTLQMGYFGDWASHTMEHAVSAVYDIPHAGGLAILFPNWMRYTLDTNVGRFKNLMLNMFDIDTEGKT
DKEIALEGIDKLSAFWTSLGAPSRLADYNIGEEKLELIADIAAKEMEHGGFGNFQKLNKDDVLAILRASL
>Mature_390_residues
MENFTYYNPTKLIFGKGQLEQLRKEFKRYGKNVLLVYGGGSIKRNGLYDQVTGILKEEGAVVHELSGVEPNPRLATVEKG
IGLCREHDIDFLLAVGGGSVIDCTKAIAAGVKYDGDAWDIFSKKVTAEDALPFGTVLTLAATGSEMNPDSVITNWETNEK
FVWGSNVTHPRFSILDPENTFTVPENQTVYGMVDMMSHVFEQYFHNVENTPLQDRMCFAVLQTVIETAPKLLEDLENYEL
RETILYAGTIALNGTLQMGYFGDWASHTMEHAVSAVYDIPHAGGLAILFPNWMRYTLDTNVGRFKNLMLNMFDIDTEGKT
DKEIALEGIDKLSAFWTSLGAPSRLADYNIGEEKLELIADIAAKEMEHGGFGNFQKLNKDDVLAILRASL

Specific function: Unknown

COG id: COG1979

COG function: function code C; Uncharacterized oxidoreductases, Fe-dependent alcohol dehydrogenase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789386, Length=374, Percent_Identity=39.0374331550802, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI48994951, Length=393, Percent_Identity=26.2086513994911, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789163, Length=395, Percent_Identity=24.8101265822785, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87082107, Length=343, Percent_Identity=24.4897959183673, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787493, Length=322, Percent_Identity=26.3975155279503, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17537053, Length=200, Percent_Identity=26, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321181, Length=387, Percent_Identity=24.5478036175711, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YUGK_BACSU (O05240)

Other databases:

- EMBL:   Z93934
- EMBL:   AL009126
- PIR:   B70011
- RefSeq:   NP_391014.1
- ProteinModelPortal:   O05240
- SMR:   O05240
- EnsemblBacteria:   EBBACT00000000992
- GeneID:   938844
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31360
- NMPDR:   fig|224308.1.peg.3139
- GenoList:   BSU31360
- GeneTree:   EBGT00050000000021
- HOGENOM:   HBG742415
- OMA:   VHMIEHS
- PhylomeDB:   O05240
- ProtClustDB:   CLSK2460951
- BioCyc:   BSUB:BSU31360-MONOMER
- InterPro:   IPR001670
- InterPro:   IPR018211

Pfam domain/function: PF00465 Fe-ADH

EC number: 1.1.-.- [C]

Molecular weight: Translated: 43407; Mature: 43407

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00913 ADH_IRON_1; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENFTYYNPTKLIFGKGQLEQLRKEFKRYGKNVLLVYGGGSIKRNGLYDQVTGILKEEGA
CCCCCEECCCEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHCCC
VVHELSGVEPNPRLATVEKGIGLCREHDIDFLLAVGGGSVIDCTKAIAAGVKYDGDAWDI
EEEHHCCCCCCCCEEEHHHHCCHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHH
FSKKVTAEDALPFGTVLTLAATGSEMNPDSVITNWETNEKFVWGSNVTHPRFSILDPENT
HHHHCCHHHCCCCCCEEEEEECCCCCCCCCEEECCCCCCEEEECCCCCCCCEEEECCCCC
FTVPENQTVYGMVDMMSHVFEQYFHNVENTPLQDRMCFAVLQTVIETAPKLLEDLENYEL
EECCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
RETILYAGTIALNGTLQMGYFGDWASHTMEHAVSAVYDIPHAGGLAILFPNWMRYTLDTN
HHHHEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCC
VGRFKNLMLNMFDIDTEGKTDKEIALEGIDKLSAFWTSLGAPSRLADYNIGEEKLELIAD
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHH
IAAKEMEHGGFGNFQKLNKDDVLAILRASL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MENFTYYNPTKLIFGKGQLEQLRKEFKRYGKNVLLVYGGGSIKRNGLYDQVTGILKEEGA
CCCCCEECCCEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHCCC
VVHELSGVEPNPRLATVEKGIGLCREHDIDFLLAVGGGSVIDCTKAIAAGVKYDGDAWDI
EEEHHCCCCCCCCEEEHHHHCCHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHH
FSKKVTAEDALPFGTVLTLAATGSEMNPDSVITNWETNEKFVWGSNVTHPRFSILDPENT
HHHHCCHHHCCCCCCEEEEEECCCCCCCCCEEECCCCCCEEEECCCCCCCCEEEECCCCC
FTVPENQTVYGMVDMMSHVFEQYFHNVENTPLQDRMCFAVLQTVIETAPKLLEDLENYEL
EECCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
RETILYAGTIALNGTLQMGYFGDWASHTMEHAVSAVYDIPHAGGLAILFPNWMRYTLDTN
HHHHEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCC
VGRFKNLMLNMFDIDTEGKTDKEIALEGIDKLSAFWTSLGAPSRLADYNIGEEKLELIAD
HHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHH
IAAKEMEHGGFGNFQKLNKDDVLAILRASL
HHHHHHHCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377