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The map label for this gene is metK

Identifier: 16080107

GI number: 16080107

Start: 3127825

End: 3129027

Strand: Reverse

Name: metK

Synonym: BSU30550

Alternate gene names: 16080107

Gene position: 3129027-3127825 (Counterclockwise)

Preceding gene: 16080109

Following gene: 16080106

Centisome position: 74.22

GC content: 45.97

Gene sequence:

>1203_bases
ATGAGTAAAAATCGTCGTTTATTTACATCAGAATCTGTTACGGAGGGGCATCCGGATAAAATCTGTGACCAGATTTCTGA
CAGCATTTTAGATGAAATTTTAAAGAAAGACCCTAACGCGCGTGTTGCTTGTGAAACATCTGTGACAACAGGTTTGGTTC
TTGTAAGCGGAGAGATCACAACTTCTACGTATGTTGACATTCCGAAAACGGTTCGCCAAACCATTAAAGAAATCGGATAC
ACACGTGCAAAATACGGATTTGATGCGGAAACTTGTGCGGTTTTAACATCAATTGATGAGCAGTCTGCTGATATCGCGAT
GGGCGTAGACCAAGCGCTTGAAGCCCGTGAAGGCACAATGAGCGACGAAGAAATTGAAGCGATTGGTGCGGGTGACCAAG
GATTAATGTTCGGTTATGCGTGCAACGAAACGAAAGAGCTTATGCCTCTTCCAATTTCACTTGCCCATAAATTAGCCCGC
CGCCTAAGTGAAGTCCGTAAAGAAGATATTCTTCCGTACCTTCGCCCTGACGGCAAAACACAGGTAACGGTTGAGTACGA
TGAAAATAACAAACCAGTCCGCATTGACGCGATTGTTATTTCAACTCAGCATCACCCTGAAATTACACTTGAGCAAATTC
AGCGCAACATTAAAGAACATGTAATCAATCCGGTTGTTCCTGAAGAGCTGATTGATGAAGAAACAAAATATTTCATCAAC
CCTACAGGACGTTTCGTAATCGGAGGCCCTCAAGGGGATGCGGGACTTACAGGACGCAAAATCATCGTTGATACGTACGG
CGGCTATGCACGCCACGGCGGAGGCGCGTTCTCAGGTAAGGACGCGACGAAGGTAGACCGTTCTGCAGCTTATGCGGCAA
GATACGTTGCGAAAAACATCGTTGCGGCTGAGCTTGCTGATTCTTGCGAAGTACAGCTTGCTTACGCGATCGGTGTTGCA
CAGCCTGTGTCAATCTCAATCAACACATTCGGTTCAGGAAAAGCTTCTGAGGAAAAACTGATTGAAGTTGTTCGCAATAA
CTTTGATTTACGACCTGCCGGCATTATCAAAATGCTTGATTTGCGCCGTCCGATCTATAAACAAACTGCTGCGTACGGCC
ACTTTGGACGTCACGATGTTGACCTTCCATGGGAGCGCACAGACAAAGCGGAGCAGCTGCGTAAAGAAGCGTTAGGAGAA
TAA

Upstream 100 bases:

>100_bases
TAACATTCTCTAATATAACTGTACATTGTATAAGAGGGAGCGAGTTCCGTATCATATATACAAGGTCTTTCGGGAGGCCT
TGTGCAGGAGGAAGCAAATC

Downstream 100 bases:

>100_bases
TTTTATAGCCGCTTACTGGTTAAGCGGCTTTCCCTTTTTTATCGTTGTATTCATGTTTATTTTTTTACATAACTGTGAAA
CCAAATACTATTCACAGCGT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSKNRRLFTSESVTEGHPDKICDQISDSILDEILKKDPNARVACETSVTTGLVLVSGEITTSTYVDIPKTVRQTIKEIGY
TRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGTMSDEEIEAIGAGDQGLMFGYACNETKELMPLPISLAHKLAR
RLSEVRKEDILPYLRPDGKTQVTVEYDENNKPVRIDAIVISTQHHPEITLEQIQRNIKEHVINPVVPEELIDEETKYFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAELADSCEVQLAYAIGVA
QPVSISINTFGSGKASEEKLIEVVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRHDVDLPWERTDKAEQLRKEALGE

Sequences:

>Translated_400_residues
MSKNRRLFTSESVTEGHPDKICDQISDSILDEILKKDPNARVACETSVTTGLVLVSGEITTSTYVDIPKTVRQTIKEIGY
TRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGTMSDEEIEAIGAGDQGLMFGYACNETKELMPLPISLAHKLAR
RLSEVRKEDILPYLRPDGKTQVTVEYDENNKPVRIDAIVISTQHHPEITLEQIQRNIKEHVINPVVPEELIDEETKYFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAELADSCEVQLAYAIGVA
QPVSISINTFGSGKASEEKLIEVVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRHDVDLPWERTDKAEQLRKEALGE
>Mature_399_residues
SKNRRLFTSESVTEGHPDKICDQISDSILDEILKKDPNARVACETSVTTGLVLVSGEITTSTYVDIPKTVRQTIKEIGYT
RAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGTMSDEEIEAIGAGDQGLMFGYACNETKELMPLPISLAHKLARR
LSEVRKEDILPYLRPDGKTQVTVEYDENNKPVRIDAIVISTQHHPEITLEQIQRNIKEHVINPVVPEELIDEETKYFINP
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAELADSCEVQLAYAIGVAQ
PVSISINTFGSGKASEEKLIEVVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRHDVDLPWERTDKAEQLRKEALGE

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=388, Percent_Identity=59.020618556701, Blast_Score=444, Evalue=1e-125,
Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=58.1151832460733, Blast_Score=432, Evalue=1e-121,
Organism=Escherichia coli, GI1789311, Length=393, Percent_Identity=63.1043256997455, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI25145633, Length=392, Percent_Identity=55.1020408163265, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=54.7557840616967, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=53.9845758354756, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=52.8497409326425, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=49.3573264781491, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=53.2544378698225, Blast_Score=350, Evalue=9e-97,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=52.7859237536657, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI32565903, Length=311, Percent_Identity=52.7331189710611, Blast_Score=318, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=55.1724137931034, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=68.1818181818182, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=55.8746736292428, Blast_Score=428, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320710, Length=395, Percent_Identity=54.1772151898734, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45552159, Length=388, Percent_Identity=54.639175257732, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580529, Length=388, Percent_Identity=54.639175257732, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580537, Length=388, Percent_Identity=54.639175257732, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580533, Length=388, Percent_Identity=54.639175257732, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580535, Length=388, Percent_Identity=54.639175257732, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580531, Length=388, Percent_Identity=54.639175257732, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580527, Length=388, Percent_Identity=54.1237113402062, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580525, Length=388, Percent_Identity=54.1237113402062, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580541, Length=376, Percent_Identity=52.9255319148936, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=53.5433070866142, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44043; Mature: 43912

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNRRLFTSESVTEGHPDKICDQISDSILDEILKKDPNARVACETSVTTGLVLVSGEIT
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCC
TSTYVDIPKTVRQTIKEIGYTRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGTM
CCEEECCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCHHHHCHHHHHHHHCCCC
SDEEIEAIGAGDQGLMFGYACNETKELMPLPISLAHKLARRLSEVRKEDILPYLRPDGKT
CHHHHHEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
QVTVEYDENNKPVRIDAIVISTQHHPEITLEQIQRNIKEHVINPVVPEELIDEETKYFIN
EEEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEC
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAELADSCEVQLAYAIGVAQPVSISINTFGSGKASEEKLIEVVRNNFDLRPAGIIKMLD
HHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
LRRPIYKQTAAYGHFGRHDVDLPWERTDKAEQLRKEALGE
HCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SKNRRLFTSESVTEGHPDKICDQISDSILDEILKKDPNARVACETSVTTGLVLVSGEIT
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCC
TSTYVDIPKTVRQTIKEIGYTRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGTM
CCEEECCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCHHHHCHHHHHHHHCCCC
SDEEIEAIGAGDQGLMFGYACNETKELMPLPISLAHKLARRLSEVRKEDILPYLRPDGKT
CHHHHHEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
QVTVEYDENNKPVRIDAIVISTQHHPEITLEQIQRNIKEHVINPVVPEELIDEETKYFIN
EEEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEC
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAELADSCEVQLAYAIGVAQPVSISINTFGSGKASEEKLIEVVRNNFDLRPAGIIKMLD
HHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
LRRPIYKQTAAYGHFGRHDVDLPWERTDKAEQLRKEALGE
HCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA