Click here to switch to the map view.

The map label for this gene is asnB

Identifier: 16080106

GI number: 16080106

Start: 3125777

End: 3127675

Strand: Reverse

Name: asnB

Synonym: BSU30540

Alternate gene names: 16080106

Gene position: 3127675-3125777 (Counterclockwise)

Preceding gene: 16080107

Following gene: 16080105

Centisome position: 74.19

GC content: 43.92

Gene sequence:

>1899_bases
ATGTGTGGATTTGTCGGGGTTTTTAACAAGCATCCGTTAGCTCAAACCGCTGATCAAGAAGAACTAATCAAACAAATGAA
CCAAATGATCGTTCACCGCGGTCCTGACAGTGATGGATATTTCCATGATGAGCACGTCGGCTTCGGATTCAGACGGCTCA
GCATTATTGATGTAGAAAATGGTGGACAGCCTTTATCATATGAAGATGAAACATATTGGATTATCTTTAACGGAGAAATC
TATAACTATATCGAACTGAGAGAAGAACTTGAAGCAAAGGGGTACACATTCAACACGGATTCGGACACAGAGGTTCTTCT
TGCGACTTACCGTCACTATAAAGAAGAAGCGGCGTCCAAACTGCGCGGTATGTTTGCCTTTCTGATTTGGAATAAAAACG
ATCATGTGCTTTATGGGGCAAGAGATCCATTCGGCATTAAACCGTTGTACTACACAACGATCAATGATCAGGTTTATTTT
GCCTCAGAGAGAAAAAGTTTAATGGTTGCTCAGAATGATATTGAAATAGATAAAGAGGCATTGCAGCAGTACATGTCTTT
CCAATTTGTTCCTGAACCAAGCACGCTTGATGCCCATGTGAAAAAAGTAGAGCCGGGTTCACAATTCACAATCCGCCCGG
ACGGCGATATCACATTCAAAACGTATTTCAAAGCGAACTTCAAGCCGGTTCAGACTGAAGAAGACAAGCTTGTGAAAGAA
GTCCGAGACGCGATCTATGACTCTGTAAACGTTCATATGAGAAGTGACGTTCCTGTCGGTTCGTTCCTGTCTGGCGGGAT
TGATTCTTCCTTTATTGTGTCTGTTGCGAAGGAATTTCATCCAAGCTTAAAAACATTCTCTGTCGGCTTTGAACAGCAGG
GCTTCAGTGAGGTTGATGTAGCGAAAGAAACGGCAGCGGCGCTGGGCATTGAAAATATCAGCAAAGTCATTTCGCCTGAG
GAATACATGAATGAGCTTCCGAAGATCGTATGGCATTTTGATGACCCGCTTGCTGATCCGGCAGCGATTCCGTTGTACTT
TGTTGCAAAAGAAGCAAAAAAACATGTTACGGTTGCTTTATCAGGTGAAGGAGCGGATGAGCTCTTCGGCGGATATAACA
TTTACCGTGAACCGCTTTCGCTTAAACCGTTTGAACGGATTCCGTCCGGCTTGAAAAAGATGCTTCTGCACGTTGCTGCC
GTTATGCCTGAAGGTATGAGAGGGAAGAGCCTGCTTGAGCGCGGCTGCACTCCGCTTCAGGATCGTTATATCGGTAACGC
GAAAATCTTTGAAGAGTCTGTCAAAAAGCAGCTGTTGAAGCATTATAATCCGAACCTTTCATATCGCGATGTGACGAAGA
CTTACTTTACAGAAAGCAGCTCGTACAGCGATATCAACAAAATGCAGTATGTCGATATCCACACTTGGATGCGCGGCGAC
ATTTTGTTAAAGGCGGATAAAATGACGATGGCGAATTCTTTGGAACTGCGTGTGCCGTTCTTGGATAAAGTCGTATTCGA
CGTCGCTTCAAAAATTCCTGATGAGCTGAAAACGAAGAACGGCACGACGAAGTATCTTCTTCGCAAAGCCGCTGAAGGAA
TTGTGCCTGAGCACGTATTAAACCGCAAAAAGCTTGGTTTCCCTGTACCGATCCGTCATTGGCTGAAAAACGAAATGAAC
GAATGGGTGCGGAATATTATTCAAGAAAGCCAAACGGATGCTTATATCCATAAGGATTATGTCCTTCAGCTTCTTGAAGA
CCATTGTGCGGACAAGGCTGATAACAGCCGTAAAATTTGGACTGTGCTAATCTTTATGATCTGGCACAGCATCAATATTG
AAAAACGGTATATGCCTGAAGAGCTGAGCCATCAGCCTAAAGAAGTCATCTTTGTGTAA

Upstream 100 bases:

>100_bases
TTCATGTTTATTTTTTTACATAACTGTGAAACCAAATACTATTCACAGCGTCTATAAATAGGGGTTCAATGATGACAATT
TTAATTATGGAGGCAATACT

Downstream 100 bases:

>100_bases
CAGACAACAAGGCTTTCGAGTAGTCGAAAGCCTTGTTGTTATCCTTTTATTTCTCCATCATCATTGATTTACCCAATCCG
TACGGAATATTCAGAAAAAT

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MCGFVGVFNKHPLAQTADQEELIKQMNQMIVHRGPDSDGYFHDEHVGFGFRRLSIIDVENGGQPLSYEDETYWIIFNGEI
YNYIELREELEAKGYTFNTDSDTEVLLATYRHYKEEAASKLRGMFAFLIWNKNDHVLYGARDPFGIKPLYYTTINDQVYF
ASERKSLMVAQNDIEIDKEALQQYMSFQFVPEPSTLDAHVKKVEPGSQFTIRPDGDITFKTYFKANFKPVQTEEDKLVKE
VRDAIYDSVNVHMRSDVPVGSFLSGGIDSSFIVSVAKEFHPSLKTFSVGFEQQGFSEVDVAKETAAALGIENISKVISPE
EYMNELPKIVWHFDDPLADPAAIPLYFVAKEAKKHVTVALSGEGADELFGGYNIYREPLSLKPFERIPSGLKKMLLHVAA
VMPEGMRGKSLLERGCTPLQDRYIGNAKIFEESVKKQLLKHYNPNLSYRDVTKTYFTESSSYSDINKMQYVDIHTWMRGD
ILLKADKMTMANSLELRVPFLDKVVFDVASKIPDELKTKNGTTKYLLRKAAEGIVPEHVLNRKKLGFPVPIRHWLKNEMN
EWVRNIIQESQTDAYIHKDYVLQLLEDHCADKADNSRKIWTVLIFMIWHSINIEKRYMPEELSHQPKEVIFV

Sequences:

>Translated_632_residues
MCGFVGVFNKHPLAQTADQEELIKQMNQMIVHRGPDSDGYFHDEHVGFGFRRLSIIDVENGGQPLSYEDETYWIIFNGEI
YNYIELREELEAKGYTFNTDSDTEVLLATYRHYKEEAASKLRGMFAFLIWNKNDHVLYGARDPFGIKPLYYTTINDQVYF
ASERKSLMVAQNDIEIDKEALQQYMSFQFVPEPSTLDAHVKKVEPGSQFTIRPDGDITFKTYFKANFKPVQTEEDKLVKE
VRDAIYDSVNVHMRSDVPVGSFLSGGIDSSFIVSVAKEFHPSLKTFSVGFEQQGFSEVDVAKETAAALGIENISKVISPE
EYMNELPKIVWHFDDPLADPAAIPLYFVAKEAKKHVTVALSGEGADELFGGYNIYREPLSLKPFERIPSGLKKMLLHVAA
VMPEGMRGKSLLERGCTPLQDRYIGNAKIFEESVKKQLLKHYNPNLSYRDVTKTYFTESSSYSDINKMQYVDIHTWMRGD
ILLKADKMTMANSLELRVPFLDKVVFDVASKIPDELKTKNGTTKYLLRKAAEGIVPEHVLNRKKLGFPVPIRHWLKNEMN
EWVRNIIQESQTDAYIHKDYVLQLLEDHCADKADNSRKIWTVLIFMIWHSINIEKRYMPEELSHQPKEVIFV
>Mature_632_residues
MCGFVGVFNKHPLAQTADQEELIKQMNQMIVHRGPDSDGYFHDEHVGFGFRRLSIIDVENGGQPLSYEDETYWIIFNGEI
YNYIELREELEAKGYTFNTDSDTEVLLATYRHYKEEAASKLRGMFAFLIWNKNDHVLYGARDPFGIKPLYYTTINDQVYF
ASERKSLMVAQNDIEIDKEALQQYMSFQFVPEPSTLDAHVKKVEPGSQFTIRPDGDITFKTYFKANFKPVQTEEDKLVKE
VRDAIYDSVNVHMRSDVPVGSFLSGGIDSSFIVSVAKEFHPSLKTFSVGFEQQGFSEVDVAKETAAALGIENISKVISPE
EYMNELPKIVWHFDDPLADPAAIPLYFVAKEAKKHVTVALSGEGADELFGGYNIYREPLSLKPFERIPSGLKKMLLHVAA
VMPEGMRGKSLLERGCTPLQDRYIGNAKIFEESVKKQLLKHYNPNLSYRDVTKTYFTESSSYSDINKMQYVDIHTWMRGD
ILLKADKMTMANSLELRVPFLDKVVFDVASKIPDELKTKNGTTKYLLRKAAEGIVPEHVLNRKKLGFPVPIRHWLKNEMN
EWVRNIIQESQTDAYIHKDYVLQLLEDHCADKADNSRKIWTVLIFMIWHSINIEKRYMPEELSHQPKEVIFV

Specific function: Main asparagine synthetase in vegetative cells

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Homo sapiens, GI168229248, Length=398, Percent_Identity=26.1306532663317, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI168229252, Length=398, Percent_Identity=26.1306532663317, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI168229250, Length=398, Percent_Identity=26.1306532663317, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI296010848, Length=368, Percent_Identity=26.6304347826087, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI296010852, Length=296, Percent_Identity=24.3243243243243, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI296010850, Length=296, Percent_Identity=24.3243243243243, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1786889, Length=507, Percent_Identity=28.7968441814596, Blast_Score=179, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI25147557, Length=400, Percent_Identity=30, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71993933, Length=400, Percent_Identity=30, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17560178, Length=395, Percent_Identity=29.620253164557, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25147560, Length=321, Percent_Identity=28.9719626168224, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6325403, Length=429, Percent_Identity=27.2727272727273, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321563, Length=428, Percent_Identity=26.8691588785047, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45553209, Length=407, Percent_Identity=30.958230958231, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASNB_BACSU (P54420)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- EMBL:   U52812
- PIR:   H69590
- RefSeq:   NP_390932.1
- ProteinModelPortal:   P54420
- SMR:   P54420
- EnsemblBacteria:   EBBACT00000003325
- GeneID:   937236
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30540
- NMPDR:   fig|224308.1.peg.3057
- GenoList:   BSU30540
- GeneTree:   EBGT00050000000005
- HOGENOM:   HBG752912
- OMA:   RHWLKNE
- PhylomeDB:   P54420
- ProtClustDB:   CLSK872028
- BioCyc:   BSUB:BSU30540-MONOMER
- BRENDA:   6.3.5.4
- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR01536

Pfam domain/function: PF00733 Asn_synthase

EC number: =6.3.5.4

Molecular weight: Translated: 72667; Mature: 72667

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS51278 GATASE_TYPE_2

Important sites: ACT_SITE 2-2

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGFVGVFNKHPLAQTADQEELIKQMNQMIVHRGPDSDGYFHDEHVGFGFRRLSIIDVEN
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEEEEECC
GGQPLSYEDETYWIIFNGEIYNYIELREELEAKGYTFNTDSDTEVLLATYRHYKEEAASK
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHH
LRGMFAFLIWNKNDHVLYGARDPFGIKPLYYTTINDQVYFASERKSLMVAQNDIEIDKEA
HHHEEEEEEECCCCCEEEECCCCCCCCCEEEEEECCEEEEECCCCEEEEECCCCCCCHHH
LQQYMSFQFVPEPSTLDAHVKKVEPGSQFTIRPDGDITFKTYFKANFKPVQTEEDKLVKE
HHHHHCEEECCCCCCCCCHHEECCCCCEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHH
VRDAIYDSVNVHMRSDVPVGSFLSGGIDSSFIVSVAKEFHPSLKTFSVGFEQQGFSEVDV
HHHHHHCCCCEEEECCCCHHHHHHCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCHHHH
AKETAAALGIENISKVISPEEYMNELPKIVWHFDDPLADPAAIPLYFVAKEAKKHVTVAL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCCCCCCCCCHHHEEEHHCCCEEEEEE
SGEGADELFGGYNIYREPLSLKPFERIPSGLKKMLLHVAAVMPEGMRGKSLLERGCTPLQ
CCCCHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHH
DRYIGNAKIFEESVKKQLLKHYNPNLSYRDVTKTYFTESSSYSDINKMQYVDIHTWMRGD
HHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCC
ILLKADKMTMANSLELRVPFLDKVVFDVASKIPDELKTKNGTTKYLLRKAAEGIVPEHVL
EEEEECCEECCCCCEEECCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHCCCCHHHH
NRKKLGFPVPIRHWLKNEMNEWVRNIIQESQTDAYIHKDYVLQLLEDHCADKADNSRKIW
CHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
TVLIFMIWHSINIEKRYMPEELSHQPKEVIFV
HHHHHHHHHHCCCHHHCCCHHHCCCCCCEEEC
>Mature Secondary Structure
MCGFVGVFNKHPLAQTADQEELIKQMNQMIVHRGPDSDGYFHDEHVGFGFRRLSIIDVEN
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEEEEEEEECC
GGQPLSYEDETYWIIFNGEIYNYIELREELEAKGYTFNTDSDTEVLLATYRHYKEEAASK
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHH
LRGMFAFLIWNKNDHVLYGARDPFGIKPLYYTTINDQVYFASERKSLMVAQNDIEIDKEA
HHHEEEEEEECCCCCEEEECCCCCCCCCEEEEEECCEEEEECCCCEEEEECCCCCCCHHH
LQQYMSFQFVPEPSTLDAHVKKVEPGSQFTIRPDGDITFKTYFKANFKPVQTEEDKLVKE
HHHHHCEEECCCCCCCCCHHEECCCCCEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHH
VRDAIYDSVNVHMRSDVPVGSFLSGGIDSSFIVSVAKEFHPSLKTFSVGFEQQGFSEVDV
HHHHHHCCCCEEEECCCCHHHHHHCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCHHHH
AKETAAALGIENISKVISPEEYMNELPKIVWHFDDPLADPAAIPLYFVAKEAKKHVTVAL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCCCCCCCCCHHHEEEHHCCCEEEEEE
SGEGADELFGGYNIYREPLSLKPFERIPSGLKKMLLHVAAVMPEGMRGKSLLERGCTPLQ
CCCCHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHH
DRYIGNAKIFEESVKKQLLKHYNPNLSYRDVTKTYFTESSSYSDINKMQYVDIHTWMRGD
HHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCC
ILLKADKMTMANSLELRVPFLDKVVFDVASKIPDELKTKNGTTKYLLRKAAEGIVPEHVL
EEEEECCEECCCCCEEECCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHCCCCHHHH
NRKKLGFPVPIRHWLKNEMNEWVRNIIQESQTDAYIHKDYVLQLLEDHCADKADNSRKIW
CHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
TVLIFMIWHSINIEKRYMPEELSHQPKEVIFV
HHHHHHHHHHCCCHHHCCCHHHCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721