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The map label for this gene is bioB

Identifier: 16080072

GI number: 16080072

Start: 3090482

End: 3091489

Strand: Reverse

Name: bioB

Synonym: BSU30200

Alternate gene names: 16080072

Gene position: 3091489-3090482 (Counterclockwise)

Preceding gene: 16080073

Following gene: 16080071

Centisome position: 73.33

GC content: 45.93

Gene sequence:

>1008_bases
ATGAATCAATGGATGGAACTCGCAGACCGGGTGCTGGCTGGAGCAGAAGTGACTGACGAAGAGGCGCTTTCAATATTACA
TTGTCCTGATGAAGATATTTTGCTATTAATGCACGGGGCTTTTCACATCAGAAAACACTTTTACGGAAAAAAAGTAAAGC
TCAATATGATTATGAATGCGAAATCCGGGCTCTGCCCGGAAAACTGCGGCTATTGTTCACAGTCTGCGATTTCGAAAGCG
CCGATTGAGTCTTACCGGATGGTGAATAAGGAAACGCTGCTTGAAGGCGCGAAGCGGGCGCACGATCTGAATATCGGCAC
ATATTGTATCGTGGCAAGCGGCAGAGGTCCGTCTAACAGAGAAGTGGATCAGGTCGTAGATGCGGTTCAGGAAATTAAAG
AGACGTATGGACTGAAGATTTGTGCATGTCTTGGACTGTTGAAGCCAGAGCAGGCGAAGCGGCTCAAAGATGCAGGAGTA
GACCGCTATAATCATAATTTGAATACGTCACAGAGAAACCATTCAAACATCACAACCTCACATACATACGATGACAGAGT
CAATACGGTTGAAATCGCAAAAGAATCGGGGCTGTCTCCGTGTTCAGGCGCCATTATCGGGATGAAGGAGACGAAACAGG
ATGTCATTGACATCGCCAAAAGCTTGAAGGCTCTTGACGCGGATTCCATTCCTGTGAATTTTTTGCATGCAATTGATGGC
ACGCCGTTAGAAGGCGTCAACGAATTAAACCCGCTGTATTGTTTAAAAGTGCTGGCGCTGTTCCGTTTTATCAATCCATC
AAAAGAAATTCGCATTTCCGGAGGAAGAGAGGTCAATCTCCGCACATTGCAGCCATTAGGGCTTTACGCCGCAAACTCCA
TTTTTGTCGGAGACTACTTAACAACTGCCGGGCAAGAGGAGACGGAGGATCATAAAATGCTGAGTGATTTAGGCTTTGAA
GTTGAATCAGTCGAAGAAATGAAGGCTAGTTTAAGTGCGAAAAGCTGA

Upstream 100 bases:

>100_bases
TTACGCCAAAGCTTGCCAACGTGACGAAAGAAACGGTTCTACATATGGTAAAAGACCATATCAATCTATCATTACTGATG
AATCAAGTGGGGGTATGAGA

Downstream 100 bases:

>100_bases
AAGAATCAATAAAAGCAATCGGTATGATGTCGATTGTTTTTATTTTTGAACAGAAAGGAGAAAATCACGTGACAATTGCA
TCGTCAACTGCATCTTCTGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNQWMELADRVLAGAEVTDEEALSILHCPDEDILLLMHGAFHIRKHFYGKKVKLNMIMNAKSGLCPENCGYCSQSAISKA
PIESYRMVNKETLLEGAKRAHDLNIGTYCIVASGRGPSNREVDQVVDAVQEIKETYGLKICACLGLLKPEQAKRLKDAGV
DRYNHNLNTSQRNHSNITTSHTYDDRVNTVEIAKESGLSPCSGAIIGMKETKQDVIDIAKSLKALDADSIPVNFLHAIDG
TPLEGVNELNPLYCLKVLALFRFINPSKEIRISGGREVNLRTLQPLGLYAANSIFVGDYLTTAGQEETEDHKMLSDLGFE
VESVEEMKASLSAKS

Sequences:

>Translated_335_residues
MNQWMELADRVLAGAEVTDEEALSILHCPDEDILLLMHGAFHIRKHFYGKKVKLNMIMNAKSGLCPENCGYCSQSAISKA
PIESYRMVNKETLLEGAKRAHDLNIGTYCIVASGRGPSNREVDQVVDAVQEIKETYGLKICACLGLLKPEQAKRLKDAGV
DRYNHNLNTSQRNHSNITTSHTYDDRVNTVEIAKESGLSPCSGAIIGMKETKQDVIDIAKSLKALDADSIPVNFLHAIDG
TPLEGVNELNPLYCLKVLALFRFINPSKEIRISGGREVNLRTLQPLGLYAANSIFVGDYLTTAGQEETEDHKMLSDLGFE
VESVEEMKASLSAKS
>Mature_335_residues
MNQWMELADRVLAGAEVTDEEALSILHCPDEDILLLMHGAFHIRKHFYGKKVKLNMIMNAKSGLCPENCGYCSQSAISKA
PIESYRMVNKETLLEGAKRAHDLNIGTYCIVASGRGPSNREVDQVVDAVQEIKETYGLKICACLGLLKPEQAKRLKDAGV
DRYNHNLNTSQRNHSNITTSHTYDDRVNTVEIAKESGLSPCSGAIIGMKETKQDVIDIAKSLKALDADSIPVNFLHAIDG
TPLEGVNELNPLYCLKVLALFRFINPSKEIRISGGREVNLRTLQPLGLYAANSIFVGDYLTTAGQEETEDHKMLSDLGFE
VESVEEMKASLSAKS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=280, Percent_Identity=35.7142857142857, Blast_Score=190, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321725, Length=319, Percent_Identity=33.5423197492163, Blast_Score=166, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36928; Mature: 36928

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQWMELADRVLAGAEVTDEEALSILHCPDEDILLLMHGAFHIRKHFYGKKVKLNMIMNA
CCHHHHHHHHHHCCCCCCHHHHHEEEECCCCCEEEEHHHHHHHHHHHCCCEEEEEEEECC
KSGLCPENCGYCSQSAISKAPIESYRMVNKETLLEGAKRAHDLNIGTYCIVASGRGPSNR
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
EVDQVVDAVQEIKETYGLKICACLGLLKPEQAKRLKDAGVDRYNHNLNTSQRNHSNITTS
CHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCC
HTYDDRVNTVEIAKESGLSPCSGAIIGMKETKQDVIDIAKSLKALDADSIPVNFLHAIDG
CCCCCCCCEEEEHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
TPLEGVNELNPLYCLKVLALFRFINPSKEIRISGGREVNLRTLQPLGLYAANSIFVGDYL
CCCCCHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCHHHCCCEEECHHH
TTAGQEETEDHKMLSDLGFEVESVEEMKASLSAKS
HCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQWMELADRVLAGAEVTDEEALSILHCPDEDILLLMHGAFHIRKHFYGKKVKLNMIMNA
CCHHHHHHHHHHCCCCCCHHHHHEEEECCCCCEEEEHHHHHHHHHHHCCCEEEEEEEECC
KSGLCPENCGYCSQSAISKAPIESYRMVNKETLLEGAKRAHDLNIGTYCIVASGRGPSNR
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
EVDQVVDAVQEIKETYGLKICACLGLLKPEQAKRLKDAGVDRYNHNLNTSQRNHSNITTS
CHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCC
HTYDDRVNTVEIAKESGLSPCSGAIIGMKETKQDVIDIAKSLKALDADSIPVNFLHAIDG
CCCCCCCCEEEEHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
TPLEGVNELNPLYCLKVLALFRFINPSKEIRISGGREVNLRTLQPLGLYAANSIFVGDYL
CCCCCHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCHHHCCCEEECHHH
TTAGQEETEDHKMLSDLGFEVESVEEMKASLSAKS
HCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA