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The map label for this gene is bioI

Identifier: 16080071

GI number: 16080071

Start: 3089226

End: 3090413

Strand: Reverse

Name: bioI

Synonym: BSU30190

Alternate gene names: 16080071

Gene position: 3090413-3089226 (Counterclockwise)

Preceding gene: 16080072

Following gene: 255767693

Centisome position: 73.31

GC content: 45.96

Gene sequence:

>1188_bases
GTGACAATTGCATCGTCAACTGCATCTTCTGAGTTTTTGAAAAACCCATATTCTTTTTACGACACATTGCGAGCTGTTCA
TCCTATCTATAAAGGGAGTTTCTTAAAATACCCGGGCTGGTATGTCACAGGATATGAAGAAACGGCTGCTATTTTGAAAG
ATGCGAGATTCAAAGTCCGCACCCCGCTGCCTGAGAGCTCAACCAAATATCAGGACCTTTCACATGTGCAAAATCAAATG
ATGCTGTTTCAGAACCAGCCTGATCATAGACGATTGCGGACGCTTGCCAGCGGAGCGTTTACGCCGAGAACGACAGAGAG
TTATCAGCCGTATATCATTGAAACTGTCCATCATTTGCTTGATCAAGTGCAAGGTAAAAAAAAGATGGAGGTCATTTCGG
ACTTTGCTTTTCCTTTAGCAAGTTTTGTCATAGCTAACATTATAGGTGTACCGGAGGAAGATAGGGAGCAATTAAAGGAG
TGGGCTGCGAGTCTCATTCAAACGATTGATTTTACCCGCTCAAGAAAGGCATTAACAGAGGGCAATATTATGGCTGTGCA
GGCTATGGCATATTTCAAAGAGCTGATTCAAAAGAGAAAACGCCACCCTCAACAGGATATGATCAGCATGCTCTTGAAGG
GGAGAGAAAAGGATAAGCTGACGGAAGAGGAGGCGGCATCTACGTGCATATTGCTGGCGATCGCCGGACATGAGACAACG
GTCAATCTCATCAGCAATTCAGTCCTTTGTCTGCTGCAGCATCCAGAACAGCTTTTGAAACTGAGAGAAAATCCAGATCT
TATTGGTACCGCAGTCGAGGAATGTTTACGCTATGAAAGCCCCACGCAAATGACAGCCAGAGTTGCGTCAGAGGATATTG
ACATCTGCGGGGTGACGATCCGTCAAGGAGAACAAGTCTATCTTTTGTTAGGAGCGGCTAATCGAGACCCTAGCATATTC
ACGAACCCCGATGTCTTCGATATTACGAGAAGTCCTAATCCGCATCTTTCATTCGGGCATGGCCATCATGTTTGCTTAGG
GTCCTCGCTGGCACGATTAGAAGCGCAAATTGCGATTAACACTCTTCTGCAGCGAATGCCCAGCCTTAATCTTGCGGATT
TTGAATGGCGGTATCGGCCGCTTTTTGGATTTCGGGCGCTTGAGGAGCTGCCGGTGACTTTTGAATAA

Upstream 100 bases:

>100_bases
AAATGAAGGCTAGTTTAAGTGCGAAAAGCTGAAAGAATCAATAAAAGCAATCGGTATGATGTCGATTGTTTTTATTTTTG
AACAGAAAGGAGAAAATCAC

Downstream 100 bases:

>100_bases
GCCTAAGAATGTGAGTGCCAAAAAAGTGTCAGCCCCGCCGAAAATGGGCAATCTATAAAAAAGGGGAGTGAACATCGTGA
AAAAAGTGCTGATCGCCGGC

Product: cytochrome P450

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTIASSTASSEFLKNPYSFYDTLRAVHPIYKGSFLKYPGWYVTGYEETAAILKDARFKVRTPLPESSTKYQDLSHVQNQM
MLFQNQPDHRRLRTLASGAFTPRTTESYQPYIIETVHHLLDQVQGKKKMEVISDFAFPLASFVIANIIGVPEEDREQLKE
WAASLIQTIDFTRSRKALTEGNIMAVQAMAYFKELIQKRKRHPQQDMISMLLKGREKDKLTEEEAASTCILLAIAGHETT
VNLISNSVLCLLQHPEQLLKLRENPDLIGTAVEECLRYESPTQMTARVASEDIDICGVTIRQGEQVYLLLGAANRDPSIF
TNPDVFDITRSPNPHLSFGHGHHVCLGSSLARLEAQIAINTLLQRMPSLNLADFEWRYRPLFGFRALEELPVTFE

Sequences:

>Translated_395_residues
MTIASSTASSEFLKNPYSFYDTLRAVHPIYKGSFLKYPGWYVTGYEETAAILKDARFKVRTPLPESSTKYQDLSHVQNQM
MLFQNQPDHRRLRTLASGAFTPRTTESYQPYIIETVHHLLDQVQGKKKMEVISDFAFPLASFVIANIIGVPEEDREQLKE
WAASLIQTIDFTRSRKALTEGNIMAVQAMAYFKELIQKRKRHPQQDMISMLLKGREKDKLTEEEAASTCILLAIAGHETT
VNLISNSVLCLLQHPEQLLKLRENPDLIGTAVEECLRYESPTQMTARVASEDIDICGVTIRQGEQVYLLLGAANRDPSIF
TNPDVFDITRSPNPHLSFGHGHHVCLGSSLARLEAQIAINTLLQRMPSLNLADFEWRYRPLFGFRALEELPVTFE
>Mature_394_residues
TIASSTASSEFLKNPYSFYDTLRAVHPIYKGSFLKYPGWYVTGYEETAAILKDARFKVRTPLPESSTKYQDLSHVQNQMM
LFQNQPDHRRLRTLASGAFTPRTTESYQPYIIETVHHLLDQVQGKKKMEVISDFAFPLASFVIANIIGVPEEDREQLKEW
AASLIQTIDFTRSRKALTEGNIMAVQAMAYFKELIQKRKRHPQQDMISMLLKGREKDKLTEEEAASTCILLAIAGHETTV
NLISNSVLCLLQHPEQLLKLRENPDLIGTAVEECLRYESPTQMTARVASEDIDICGVTIRQGEQVYLLLGAANRDPSIFT
NPDVFDITRSPNPHLSFGHGHHVCLGSSLARLEAQIAINTLLQRMPSLNLADFEWRYRPLFGFRALEELPVTFE

Specific function: Catalyzes the C-C bond cleavage of fatty acid linked to acyl carrier protein (ACP) to generate pimelic acid for biotin biosynthesis. It has high affinity for long-chain fatty acids with the greatest affinity for myristic acid

COG id: COG2124

COG function: function code Q; Cytochrome P450

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome P450 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOI_BACSU (P53554)

Other databases:

- EMBL:   U51868
- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   G69594
- RefSeq:   NP_390897.1
- PDB:   3EJB
- PDB:   3EJD
- PDB:   3EJE
- PDBsum:   3EJB
- PDBsum:   3EJD
- PDBsum:   3EJE
- ProteinModelPortal:   P53554
- SMR:   P53554
- DIP:   DIP-46307N
- EnsemblBacteria:   EBBACT00000003521
- GeneID:   935928
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30190
- NMPDR:   fig|224308.1.peg.3022
- GenoList:   BSU30190
- GeneTree:   EBGT00050000001046
- HOGENOM:   HBG695262
- OMA:   MTIASST
- ProtClustDB:   CLSK887747
- BioCyc:   BSUB:BSU30190-MONOMER
- InterPro:   IPR001128
- InterPro:   IPR002397
- InterPro:   IPR017972
- Gene3D:   G3DSA:1.10.630.10
- PANTHER:   PTHR19383
- PRINTS:   PR00359
- PRINTS:   PR00385

Pfam domain/function: PF00067 p450; SSF48264 Cytochrome_P450

EC number: NA

Molecular weight: Translated: 44866; Mature: 44735

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00086 CYTOCHROME_P450

Important sites: BINDING 60-60 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIASSTASSEFLKNPYSFYDTLRAVHPIYKGSFLKYPGWYVTGYEETAAILKDARFKVR
CCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCEECCCEEEECHHHHHHHHHHCCEEEE
TPLPESSTKYQDLSHVQNQMMLFQNQPDHRRLRTLASGAFTPRTTESYQPYIIETVHHLL
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
DQVQGKKKMEVISDFAFPLASFVIANIIGVPEEDREQLKEWAASLIQTIDFTRSRKALTE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNIMAVQAMAYFKELIQKRKRHPQQDMISMLLKGREKDKLTEEEAASTCILLAIAGHETT
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHCCCHHHHCCEEEEEEEECCCHH
VNLISNSVLCLLQHPEQLLKLRENPDLIGTAVEECLRYESPTQMTARVASEDIDICGVTI
HHHHHCCHHHHHCCHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEE
RQGEQVYLLLGAANRDPSIFTNPDVFDITRSPNPHLSFGHGHHVCLGSSLARLEAQIAIN
ECCCEEEEEEEECCCCCCCCCCCCEEEECCCCCCCEECCCCCCEEHHHHHHHHHHHHHHH
TLLQRMPSLNLADFEWRYRPLFGFRALEELPVTFE
HHHHHCCCCCCCCCCEEECHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TIASSTASSEFLKNPYSFYDTLRAVHPIYKGSFLKYPGWYVTGYEETAAILKDARFKVR
CCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCEECCCEEEECHHHHHHHHHHCCEEEE
TPLPESSTKYQDLSHVQNQMMLFQNQPDHRRLRTLASGAFTPRTTESYQPYIIETVHHLL
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
DQVQGKKKMEVISDFAFPLASFVIANIIGVPEEDREQLKEWAASLIQTIDFTRSRKALTE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNIMAVQAMAYFKELIQKRKRHPQQDMISMLLKGREKDKLTEEEAASTCILLAIAGHETT
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHCCCHHHHCCEEEEEEEECCCHH
VNLISNSVLCLLQHPEQLLKLRENPDLIGTAVEECLRYESPTQMTARVASEDIDICGVTI
HHHHHCCHHHHHCCHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEE
RQGEQVYLLLGAANRDPSIFTNPDVFDITRSPNPHLSFGHGHHVCLGSSLARLEAQIAIN
ECCCEEEEEEEECCCCCCCCCCCCEEEECCCCCCCEECCCCCCEEHHHHHHHHHHHHHHH
TLLQRMPSLNLADFEWRYRPLFGFRALEELPVTFE
HHHHHCCCCCCCCCCEEECHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377