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The map label for this gene is bioD

Identifier: 16080073

GI number: 16080073

Start: 3091492

End: 3092187

Strand: Reverse

Name: bioD

Synonym: BSU30210

Alternate gene names: 16080073

Gene position: 3092187-3091492 (Counterclockwise)

Preceding gene: 16080074

Following gene: 16080072

Centisome position: 73.35

GC content: 44.25

Gene sequence:

>696_bases
TTGAGGGGTTTTTTTGTGACGGGAACTGATACAGAAGTAGGGAAAACGGTTATATCCAGCGGTCTTGCTGCCTTATTGAA
AGACAATAATAGACATGTCGGGGTGTATAAACCATTTTTAAGCGGGATATCGCGCCATCATCCAGATAGTGATACAAGTT
TGCTGAAAGATATGTCGCAGACCAGTCTTTCTCATGAAGACATTACGCCTTTTGCCTTCAAGGCGCCGCTTGCACCATAC
GTTGCAGGGAAACTTGAGGGAAAGACTGTCACCATGGAAGAGGTTTTAAGCCATTGGGGGCGGATTAGAGAAAAACATGA
ATGCTTCATCGTAGAAGGTGCAGGCGGTATTTCTGTGCCATTGGGAGAGGACTATTTGGTCAGTCATGTCATAAAAGCGT
TGCAGCTTCCCATGATTATTGTGGCGCGTCCTCGCCTTGGAACCATTAATCATACCTTTTTAACTGTCAAATATGCAGAA
AGCATGGGGCTTCCAATCGCCGGAATTATCATCAATGGAATCAGTGACTCTCCTGATGAAGATGAAAAAACCAATCCTGA
GATGATTGAGCGCTTATGCGGTGTGCCGATTTTAGGGGTTACGCCAAAGCTTGCCAACGTGACGAAAGAAACGGTTCTAC
ATATGGTAAAAGACCATATCAATCTATCATTACTGATGAATCAAGTGGGGGTATGA

Upstream 100 bases:

>100_bases
AAAGCCGGATTCGAATTACAATCACGTCTGACCACAGTATGGGTGATATTGATCATTTGCTGCAAACATTTCATTCAATC
GGAAAGGAGCTGCACATCAT

Downstream 100 bases:

>100_bases
GAATGAATCAATGGATGGAACTCGCAGACCGGGTGCTGGCTGGAGCAGAAGTGACTGACGAAGAGGCGCTTTCAATATTA
CATTGTCCTGATGAAGATAT

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRGFFVTGTDTEVGKTVISSGLAALLKDNNRHVGVYKPFLSGISRHHPDSDTSLLKDMSQTSLSHEDITPFAFKAPLAPY
VAGKLEGKTVTMEEVLSHWGRIREKHECFIVEGAGGISVPLGEDYLVSHVIKALQLPMIIVARPRLGTINHTFLTVKYAE
SMGLPIAGIIINGISDSPDEDEKTNPEMIERLCGVPILGVTPKLANVTKETVLHMVKDHINLSLLMNQVGV

Sequences:

>Translated_231_residues
MRGFFVTGTDTEVGKTVISSGLAALLKDNNRHVGVYKPFLSGISRHHPDSDTSLLKDMSQTSLSHEDITPFAFKAPLAPY
VAGKLEGKTVTMEEVLSHWGRIREKHECFIVEGAGGISVPLGEDYLVSHVIKALQLPMIIVARPRLGTINHTFLTVKYAE
SMGLPIAGIIINGISDSPDEDEKTNPEMIERLCGVPILGVTPKLANVTKETVLHMVKDHINLSLLMNQVGV
>Mature_231_residues
MRGFFVTGTDTEVGKTVISSGLAALLKDNNRHVGVYKPFLSGISRHHPDSDTSLLKDMSQTSLSHEDITPFAFKAPLAPY
VAGKLEGKTVTMEEVLSHWGRIREKHECFIVEGAGGISVPLGEDYLVSHVIKALQLPMIIVARPRLGTINHTFLTVKYAE
SMGLPIAGIIINGISDSPDEDEKTNPEMIERLCGVPILGVTPKLANVTKETVLHMVKDHINLSLLMNQVGV

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=228, Percent_Identity=31.5789473684211, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI87081944, Length=179, Percent_Identity=30.1675977653631, Blast_Score=78, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324385, Length=186, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 25161; Mature: 25161

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGFFVTGTDTEVGKTVISSGLAALLKDNNRHVGVYKPFLSGISRHHPDSDTSLLKDMSQ
CCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHH
TSLSHEDITPFAFKAPLAPYVAGKLEGKTVTMEEVLSHWGRIREKHECFIVEGAGGISVP
HHCCCCCCCCHHECCCCCHHHHCCCCCCEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCC
LGEDYLVSHVIKALQLPMIIVARPRLGTINHTFLTVKYAESMGLPIAGIIINGISDSPDE
CCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEHHHCCCCEEHEEEECCCCCCCC
DEKTNPEMIERLCGVPILGVTPKLANVTKETVLHMVKDHINLSLLMNQVGV
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRGFFVTGTDTEVGKTVISSGLAALLKDNNRHVGVYKPFLSGISRHHPDSDTSLLKDMSQ
CCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHH
TSLSHEDITPFAFKAPLAPYVAGKLEGKTVTMEEVLSHWGRIREKHECFIVEGAGGISVP
HHCCCCCCCCHHECCCCCHHHHCCCCCCEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCC
LGEDYLVSHVIKALQLPMIIVARPRLGTINHTFLTVKYAESMGLPIAGIIINGISDSPDE
CCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEHHHCCCCEEHEEEECCCCCCCC
DEKTNPEMIERLCGVPILGVTPKLANVTKETVLHMVKDHINLSLLMNQVGV
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA