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The map label for this gene is tcyL

Identifier: 16079988

GI number: 16079988

Start: 3005859

End: 3006578

Strand: Reverse

Name: tcyL

Synonym: BSU29360

Alternate gene names: 16079988

Gene position: 3006578-3005859 (Counterclockwise)

Preceding gene: 16079989

Following gene: 16079987

Centisome position: 71.32

GC content: 43.75

Gene sequence:

>720_bases
ATGGAAAAAGCGTTTGATATGAACATGATTGGAGATTTTGTGCCGACGTTAACCGCTTATTTACCGGTTACATTATACAT
TTTAACACTGTCCTTGCTTTTCGGTTTTGTATTAGGACTCTTTCTGGCACTGCCCCGCATCTATAATATTCCAATTGTAA
ATCAATTGGCAAAAGTGTATATCTCGTTTTTCAGGGGAACGCCTATCATGGTGCAGCTTTTCATCGTGTTTTACGGGATC
CCGGCGCTTACGGGGCTTATCGGGATTGATACATCAAAAATGGACCCGTTTTATGCTGCTGTCGCAACCTACGCGTTAAG
CAATGCAGCGGCAGCCGCAGAAATCATTCGAGCGGGGGTGGGCAGTGTAGATAAAGGCCAAACGGAAGCGGCGTACAGTA
TTGGCTTAAGCGGTAGTCAGGCCTTCAGGCGAATCGTTCTGCCCCAGGCACTTGTCCAGGCGTTTCCGAATATGGGAAAC
ATGGTGATCAGTTCTTTAAAAGATACGTCTCTAGCCTTTTCAATAGGTGTTATGGATATGTCCGGAAGGGGCCAAACACT
GATTACCTCAAGCAACCATTCTCTTGAAGTTTACATTGCGCTATCGATTGTTTATTATGCTGTAGCGGTTTTATTCGAAT
GGTTCTTTCGAGTGGCAGAAAAACGAATCAAGAAGAATCAAACAAGGATTGTTACTGTTTTTGACATGAACATACATTGA

Upstream 100 bases:

>100_bases
GGAAATCATTGATGACGGAACGTTGAAAAGGCTCAGTTTGAAATGGCTTGGTGACGACTATTCCAAAGAGCAGTATTAGG
TAAAAAGAGAGGGAGCAGTG

Downstream 100 bases:

>100_bases
TATATTTCTTCGGATGAGGGGGGAGAAGATTGCAATTTGACTTTCCATTTATCGTTTCCGCCATGAAAGAAATGGTAAAG
ACCATTCCTCTGACATTGAT

Product: sulfur-containing amino acid ABC transporter permease

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MEKAFDMNMIGDFVPTLTAYLPVTLYILTLSLLFGFVLGLFLALPRIYNIPIVNQLAKVYISFFRGTPIMVQLFIVFYGI
PALTGLIGIDTSKMDPFYAAVATYALSNAAAAAEIIRAGVGSVDKGQTEAAYSIGLSGSQAFRRIVLPQALVQAFPNMGN
MVISSLKDTSLAFSIGVMDMSGRGQTLITSSNHSLEVYIALSIVYYAVAVLFEWFFRVAEKRIKKNQTRIVTVFDMNIH

Sequences:

>Translated_239_residues
MEKAFDMNMIGDFVPTLTAYLPVTLYILTLSLLFGFVLGLFLALPRIYNIPIVNQLAKVYISFFRGTPIMVQLFIVFYGI
PALTGLIGIDTSKMDPFYAAVATYALSNAAAAAEIIRAGVGSVDKGQTEAAYSIGLSGSQAFRRIVLPQALVQAFPNMGN
MVISSLKDTSLAFSIGVMDMSGRGQTLITSSNHSLEVYIALSIVYYAVAVLFEWFFRVAEKRIKKNQTRIVTVFDMNIH
>Mature_239_residues
MEKAFDMNMIGDFVPTLTAYLPVTLYILTLSLLFGFVLGLFLALPRIYNIPIVNQLAKVYISFFRGTPIMVQLFIVFYGI
PALTGLIGIDTSKMDPFYAAVATYALSNAAAAAEIIRAGVGSVDKGQTEAAYSIGLSGSQAFRRIVLPQALVQAFPNMGN
MVISSLKDTSLAFSIGVMDMSGRGQTLITSSNHSLEVYIALSIVYYAVAVLFEWFFRVAEKRIKKNQTRIVTVFDMNIH

Specific function: Part of the ABC transporter complex tcyJKLMN involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable). Is also involved in cystathionine, djenkolate, and S-methylcysteine transport

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1787030, Length=220, Percent_Identity=35.4545454545455, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1788226, Length=205, Percent_Identity=39.5121951219512, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1786874, Length=208, Percent_Identity=26.9230769230769, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082239, Length=211, Percent_Identity=30.3317535545024, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788645, Length=205, Percent_Identity=31.219512195122, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787086, Length=221, Percent_Identity=25.7918552036199, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1786873, Length=207, Percent_Identity=27.536231884058, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787087, Length=237, Percent_Identity=26.5822784810127, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788646, Length=187, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48994930, Length=164, Percent_Identity=31.7073170731707, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TCYL_BACSU (O34315)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   F69996
- RefSeq:   NP_390814.1
- ProteinModelPortal:   O34315
- EnsemblBacteria:   EBBACT00000001562
- GeneID:   936115
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29360
- NMPDR:   fig|224308.1.peg.2939
- GenoList:   BSU29360
- GeneTree:   EBGT00070000031979
- HOGENOM:   HBG704821
- OMA:   FRGTPIM
- PhylomeDB:   O34315
- ProtClustDB:   CLSK887716
- BioCyc:   BSUB:BSU29360-MONOMER
- InterPro:   IPR010065
- InterPro:   IPR000515
- TIGRFAMs:   TIGR01726

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 26239; Mature: 26239

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x110ecb28)-; HASH(0x119ff7f4)-; HASH(0x10bb67a8)-; HASH(0x1168e634)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKAFDMNMIGDFVPTLTAYLPVTLYILTLSLLFGFVLGLFLALPRIYNIPIVNQLAKVY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ISFFRGTPIMVQLFIVFYGIPALTGLIGIDTSKMDPFYAAVATYALSNAAAAAEIIRAGV
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GSVDKGQTEAAYSIGLSGSQAFRRIVLPQALVQAFPNMGNMVISSLKDTSLAFSIGVMDM
CCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEEEE
SGRGQTLITSSNHSLEVYIALSIVYYAVAVLFEWFFRVAEKRIKKNQTRIVTVFDMNIH
CCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCH
>Mature Secondary Structure
MEKAFDMNMIGDFVPTLTAYLPVTLYILTLSLLFGFVLGLFLALPRIYNIPIVNQLAKVY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ISFFRGTPIMVQLFIVFYGIPALTGLIGIDTSKMDPFYAAVATYALSNAAAAAEIIRAGV
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GSVDKGQTEAAYSIGLSGSQAFRRIVLPQALVQAFPNMGNMVISSLKDTSLAFSIGVMDM
CCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEEEEE
SGRGQTLITSSNHSLEVYIALSIVYYAVAVLFEWFFRVAEKRIKKNQTRIVTVFDMNIH
CCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377