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The map label for this gene is tcyK

Identifier: 16079989

GI number: 16079989

Start: 3006600

End: 3007412

Strand: Reverse

Name: tcyK

Synonym: BSU29370

Alternate gene names: 16079989

Gene position: 3007412-3006600 (Counterclockwise)

Preceding gene: 16079990

Following gene: 16079988

Centisome position: 71.34

GC content: 41.45

Gene sequence:

>813_bases
ATGAAAACGAAAACAGCATTCATGGCAATATTATTTTCGCTTATAACAGTTCTGTCAGCTTGCGGTGCAGGCAGTCAAAC
GACGGGAGCCGGGCAAAAGAAGGTACAGACGATCACGGTCGGAACTGGAACCCAATTTCCGAATATTTGCTTTATCGATG
AAAAAGGCGATTTAACCGGTTATGATGTCGAACTGATCAAAGAGCTTGATAAGCGCCTGCCACATTATAAATTCACATTT
AAAACGATGGAATTTTCCAACCTGCTTGTCAGCCTCGGACAGCATAAAGTCGATATCGTGGCACATCAAATGGAGAAAAG
CAAAGAGCGTGAGAAAAAATTTCTGTTTAACAAGGTTGCTTACAACCATTTTCCTTTAAAAATTACTGTGCTTCAAAATA
ACGATACGATCCGTGGAATTGAAGACTTAAAAGGAAAACGGGTGATTACGAGTGCAACCTCCAACGGAGCGCTTGTACTG
AAAAAATGGAATGAGGATAATGGACGGCCGTTTGAGATTGCCTATGAGGGCCAAGGCGCAAATGAGACTGCGAATCAGCT
GAAATCGGGGCGTGCGGACGCAACGATCTCAACGCCTTTTGCAGTAGACTTTCAAAACAAGACGAGCACTATTAAAGAAA
AAACCGTCGGTAATGTGCTTTCAAATGCCAAAGTGTATTTTATGTTTAACAAAAATGAACAAACGTTAAGTGACGATATT
GATAAAGCCTTACAGGAAATCATTGATGACGGAACGTTGAAAAGGCTCAGTTTGAAATGGCTTGGTGACGACTATTCCAA
AGAGCAGTATTAG

Upstream 100 bases:

>100_bases
AAAAAGCTGGAGAAAAACGGAACGCTAAAAAAACTAAGCCGAAAATGGCTCGGGGCAGATTATTCAAAATCAAGTTTTGA
AAAATAAATGGGGCGATGAG

Downstream 100 bases:

>100_bases
GTAAAAAGAGAGGGAGCAGTGATGGAAAAAGCGTTTGATATGAACATGATTGGAGATTTTGTGCCGACGTTAACCGCTTA
TTTACCGGTTACATTATACA

Product: sulfur-containing amino acid ABC transporter binding lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKTKTAFMAILFSLITVLSACGAGSQTTGAGQKKVQTITVGTGTQFPNICFIDEKGDLTGYDVELIKELDKRLPHYKFTF
KTMEFSNLLVSLGQHKVDIVAHQMEKSKEREKKFLFNKVAYNHFPLKITVLQNNDTIRGIEDLKGKRVITSATSNGALVL
KKWNEDNGRPFEIAYEGQGANETANQLKSGRADATISTPFAVDFQNKTSTIKEKTVGNVLSNAKVYFMFNKNEQTLSDDI
DKALQEIIDDGTLKRLSLKWLGDDYSKEQY

Sequences:

>Translated_270_residues
MKTKTAFMAILFSLITVLSACGAGSQTTGAGQKKVQTITVGTGTQFPNICFIDEKGDLTGYDVELIKELDKRLPHYKFTF
KTMEFSNLLVSLGQHKVDIVAHQMEKSKEREKKFLFNKVAYNHFPLKITVLQNNDTIRGIEDLKGKRVITSATSNGALVL
KKWNEDNGRPFEIAYEGQGANETANQLKSGRADATISTPFAVDFQNKTSTIKEKTVGNVLSNAKVYFMFNKNEQTLSDDI
DKALQEIIDDGTLKRLSLKWLGDDYSKEQY
>Mature_270_residues
MKTKTAFMAILFSLITVLSACGAGSQTTGAGQKKVQTITVGTGTQFPNICFIDEKGDLTGYDVELIKELDKRLPHYKFTF
KTMEFSNLLVSLGQHKVDIVAHQMEKSKEREKKFLFNKVAYNHFPLKITVLQNNDTIRGIEDLKGKRVITSATSNGALVL
KKWNEDNGRPFEIAYEGQGANETANQLKSGRADATISTPFAVDFQNKTSTIKEKTVGNVLSNAKVYFMFNKNEQTLSDDI
DKALQEIIDDGTLKRLSLKWLGDDYSKEQY

Specific function: Part of the ABC transporter complex tcyJKLMN involved in L-cystine import. Is also involved in cystathionine, djenkolate, and S-methylcysteine transport

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family

Homologues:

Organism=Escherichia coli, GI1788228, Length=239, Percent_Identity=30.1255230125523, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788648, Length=239, Percent_Identity=25.1046025104602, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787031, Length=229, Percent_Identity=27.0742358078603, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788649, Length=244, Percent_Identity=23.7704918032787, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787085, Length=235, Percent_Identity=24.6808510638298, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TCYK_BACSU (O34852)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- PIR:   E69996
- RefSeq:   NP_390815.1
- PDB:   2O1M
- PDBsum:   2O1M
- ProteinModelPortal:   O34852
- SMR:   O34852
- EnsemblBacteria:   EBBACT00000000443
- GeneID:   937359
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU29370
- NMPDR:   fig|224308.1.peg.2940
- GenoList:   BSU29370
- GeneTree:   EBGT00070000032071
- HOGENOM:   HBG698334
- OMA:   FPLKITV
- ProtClustDB:   CLSK887717
- BioCyc:   BSUB:BSU29370-MONOMER
- InterPro:   IPR001638
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3

EC number: NA

Molecular weight: Translated: 30241; Mature: 30241

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS01039 SBP_BACTERIAL_3; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKTAFMAILFSLITVLSACGAGSQTTGAGQKKVQTITVGTGTQFPNICFIDEKGDLTG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCC
YDVELIKELDKRLPHYKFTFKTMEFSNLLVSLGQHKVDIVAHQMEKSKEREKKFLFNKVA
CHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YNHFPLKITVLQNNDTIRGIEDLKGKRVITSATSNGALVLKKWNEDNGRPFEIAYEGQGA
CCCCCEEEEEEECCCCCCCHHHCCCCEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCC
NETANQLKSGRADATISTPFAVDFQNKTSTIKEKTVGNVLSNAKVYFMFNKNEQTLSDDI
CHHHHHHHCCCCCCEEECCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
DKALQEIIDDGTLKRLSLKWLGDDYSKEQY
HHHHHHHHCCCCEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKTKTAFMAILFSLITVLSACGAGSQTTGAGQKKVQTITVGTGTQFPNICFIDEKGDLTG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCCCC
YDVELIKELDKRLPHYKFTFKTMEFSNLLVSLGQHKVDIVAHQMEKSKEREKKFLFNKVA
CHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YNHFPLKITVLQNNDTIRGIEDLKGKRVITSATSNGALVLKKWNEDNGRPFEIAYEGQGA
CCCCCEEEEEEECCCCCCCHHHCCCCEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCC
NETANQLKSGRADATISTPFAVDFQNKTSTIKEKTVGNVLSNAKVYFMFNKNEQTLSDDI
CHHHHHHHCCCCCCEEECCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
DKALQEIIDDGTLKRLSLKWLGDDYSKEQY
HHHHHHHHCCCCEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377