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The map label for this gene is glcF

Identifier: 16079921

GI number: 16079921

Start: 2934594

End: 2935928

Strand: Direct

Name: glcF

Synonym: BSU28690

Alternate gene names: 16079921

Gene position: 2934594-2935928 (Clockwise)

Preceding gene: 16079920

Following gene: 16079935

Centisome position: 69.61

GC content: 48.61

Gene sequence:

>1335_bases
ATGACAACAGAAAAAGAAATGAAACAGATCCAAAATGAATTTAAAGAGCGTATGGATGAAGGAGAGTTATTAAATTGTAT
GCGCTGCGGCTTCTGTCTTCCTTCCTGTCCGACCTATATCGAATCGGGATTTCAGGAAACACACTCTCCGCGTGGGCGCA
TCGCCTTAATGAAAGCCGTAGCCGACGGTATGATTGAACCGGATGAAGATGTCGAACGGTCGCTGTCACTTTGCCTCGGC
TGCCGCGCCTGTGAGCCTGTATGCCCGTCTGGCGTGAAATACGGGCAGCTGCTTGAGGAAGCCAGAGATATTATCCACCA
AAATAAAAAACACTCTCTTGGTGAACGTGTCATGCGAAAGACGGCTTTTCACGAGCTGTTTCCGCATCAAAACCGCATGC
GCTCAGCTGTAAGCCTTATCGGTTTGTATCAGCGTTCAGGACTGCAAACCGCTGTGCGAAAATCAGGCATGCTTCGGGTC
CTGCCTGAGCATTTGCGGACGATGGAAGCTGTTCTTCCTGATGTTCCAAAAAGCAAAGACATGAAACATCGGCCGCGTTT
CCTGCCTTCCATTGGCCCCATGAAAAAACGGGTCGCCTTTTTTTCGGGCTGCTTAATGGATACAATGTTTTTACCGACCA
ATAACGCCACATTGAAGCTTTTACAGCTGGCAGGCTGCGACATTGTCATTCCGCCTGAACAGGCTTGCTGCGGGGCGCTT
CATGGACACAGCGGTGAAAAAAACACAGGGAAAGAGCTCGCGAAACAAAATATCGCTGCTTTCGAAGCATTAGACGTGGA
TGCAGTCATTACAAACGCAGGAGGATGCGGAGCCTTTCTCACTGAATATGATCATTTGCTGAAGGATGACCCTGAGTGGT
CGGAACGGGCTGCCGCTTTTGTTCAAAAGCTGAAGGATTTTTCTTCTGTTTTGGTTGAACTCGATTTCCATCAAATGGAT
CTAGCACTCGAGACGCCTCAGGTCGTTACCTACCAAGATTCATGTCATCTAAGAAATGTGATGCATACGTCACTCGAGCC
CCGCCAGCTTCTTAAAAGCATCAAAGGGGCGGAATTCAAAGAAATGGAGAAAGCAGACAGCTGCTGCGGGTCAGCGGGAA
TTTACAACATCGTCGAAGTTGAAATGTCTATGAAAATACTGGACAGCAAAATGGCTGCCGTCAAAGCAACCGAAGCGATC
CTCATTGTAACAGCAAATCCTGGCTGCCTGCTGCAGATGAAGCTCGGCATTGAACGCGAAGGACTAAGCGGTAAAGTCAG
AGCCGTTCACCTGGCTGATTTGCTATTAGAAGCAGCCGGGCACAAAACATCATGA

Upstream 100 bases:

>100_bases
ATGCGATGAAAGCGTTGAAAGCGGCATTTGACCCCCGGAACATATTAAATCCCGGCAAAATGTTTGCGAAAGATGCCAGA
AAACGTGTGGTGGCGGAAAG

Downstream 100 bases:

>100_bases
GAAAGCCCAAAACAGACATTGTTTTGGGCTTTTGTGCGTTATTCCATCGGACTGCAAATTTCGATATAATGTCCATCCGG
ATCAGCGATATAGGCGACCG

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MTTEKEMKQIQNEFKERMDEGELLNCMRCGFCLPSCPTYIESGFQETHSPRGRIALMKAVADGMIEPDEDVERSLSLCLG
CRACEPVCPSGVKYGQLLEEARDIIHQNKKHSLGERVMRKTAFHELFPHQNRMRSAVSLIGLYQRSGLQTAVRKSGMLRV
LPEHLRTMEAVLPDVPKSKDMKHRPRFLPSIGPMKKRVAFFSGCLMDTMFLPTNNATLKLLQLAGCDIVIPPEQACCGAL
HGHSGEKNTGKELAKQNIAAFEALDVDAVITNAGGCGAFLTEYDHLLKDDPEWSERAAAFVQKLKDFSSVLVELDFHQMD
LALETPQVVTYQDSCHLRNVMHTSLEPRQLLKSIKGAEFKEMEKADSCCGSAGIYNIVEVEMSMKILDSKMAAVKATEAI
LIVTANPGCLLQMKLGIEREGLSGKVRAVHLADLLLEAAGHKTS

Sequences:

>Translated_444_residues
MTTEKEMKQIQNEFKERMDEGELLNCMRCGFCLPSCPTYIESGFQETHSPRGRIALMKAVADGMIEPDEDVERSLSLCLG
CRACEPVCPSGVKYGQLLEEARDIIHQNKKHSLGERVMRKTAFHELFPHQNRMRSAVSLIGLYQRSGLQTAVRKSGMLRV
LPEHLRTMEAVLPDVPKSKDMKHRPRFLPSIGPMKKRVAFFSGCLMDTMFLPTNNATLKLLQLAGCDIVIPPEQACCGAL
HGHSGEKNTGKELAKQNIAAFEALDVDAVITNAGGCGAFLTEYDHLLKDDPEWSERAAAFVQKLKDFSSVLVELDFHQMD
LALETPQVVTYQDSCHLRNVMHTSLEPRQLLKSIKGAEFKEMEKADSCCGSAGIYNIVEVEMSMKILDSKMAAVKATEAI
LIVTANPGCLLQMKLGIEREGLSGKVRAVHLADLLLEAAGHKTS
>Mature_443_residues
TTEKEMKQIQNEFKERMDEGELLNCMRCGFCLPSCPTYIESGFQETHSPRGRIALMKAVADGMIEPDEDVERSLSLCLGC
RACEPVCPSGVKYGQLLEEARDIIHQNKKHSLGERVMRKTAFHELFPHQNRMRSAVSLIGLYQRSGLQTAVRKSGMLRVL
PEHLRTMEAVLPDVPKSKDMKHRPRFLPSIGPMKKRVAFFSGCLMDTMFLPTNNATLKLLQLAGCDIVIPPEQACCGALH
GHSGEKNTGKELAKQNIAAFEALDVDAVITNAGGCGAFLTEYDHLLKDDPEWSERAAAFVQKLKDFSSVLVELDFHQMDL
ALETPQVVTYQDSCHLRNVMHTSLEPRQLLKSIKGAEFKEMEKADSCCGSAGIYNIVEVEMSMKILDSKMAAVKATEAIL
IVTANPGCLLQMKLGIEREGLSGKVRAVHLADLLLEAAGHKTS

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI48994913, Length=390, Percent_Identity=32.8205128205128, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1786497, Length=239, Percent_Identity=30.1255230125523, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLCF_BACSU (P94534)

Other databases:

- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   E69984
- RefSeq:   NP_390747.1
- ProteinModelPortal:   P94534
- SMR:   P94534
- EnsemblBacteria:   EBBACT00000001035
- GeneID:   937435
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28690
- NMPDR:   fig|224308.1.peg.2872
- GenoList:   BSU28690
- GeneTree:   EBGT00050000002055
- HOGENOM:   HBG646784
- OMA:   GRIYLMD
- PhylomeDB:   P94534
- ProtClustDB:   CLSK887696
- BioCyc:   BSUB:BSU28690-MONOMER
- GO:   GO:0006810
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257
- Gene3D:   G3DSA:1.10.1060.10
- PIRSF:   PIRSF000139

Pfam domain/function: PF02754 CCG

EC number: NA

Molecular weight: Translated: 49206; Mature: 49075

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEKEMKQIQNEFKERMDEGELLNCMRCGFCLPSCPTYIESGFQETHSPRGRIALMKAV
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
ADGMIEPDEDVERSLSLCLGCRACEPVCPSGVKYGQLLEEARDIIHQNKKHSLGERVMRK
HCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TAFHELFPHQNRMRSAVSLIGLYQRSGLQTAVRKSGMLRVLPEHLRTMEAVLPDVPKSKD
HHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
MKHRPRFLPSIGPMKKRVAFFSGCLMDTMFLPTNNATLKLLQLAGCDIVIPPEQACCGAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCEEECCCHHHHHHH
HGHSGEKNTGKELAKQNIAAFEALDVDAVITNAGGCGAFLTEYDHLLKDDPEWSERAAAF
CCCCCCCCHHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHH
VQKLKDFSSVLVELDFHQMDLALETPQVVTYQDSCHLRNVMHTSLEPRQLLKSIKGAEFK
HHHHHHHHHHHHHHCHHHHHHEECCCCEEEECCCHHHHHHHHCCCCHHHHHHHHCCCCHH
EMEKADSCCGSAGIYNIVEVEMSMKILDSKMAAVKATEAILIVTANPGCLLQMKLGIERE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCC
GLSGKVRAVHLADLLLEAAGHKTS
CCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTEKEMKQIQNEFKERMDEGELLNCMRCGFCLPSCPTYIESGFQETHSPRGRIALMKAV
CCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
ADGMIEPDEDVERSLSLCLGCRACEPVCPSGVKYGQLLEEARDIIHQNKKHSLGERVMRK
HCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TAFHELFPHQNRMRSAVSLIGLYQRSGLQTAVRKSGMLRVLPEHLRTMEAVLPDVPKSKD
HHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
MKHRPRFLPSIGPMKKRVAFFSGCLMDTMFLPTNNATLKLLQLAGCDIVIPPEQACCGAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCEEECCCHHHHHHH
HGHSGEKNTGKELAKQNIAAFEALDVDAVITNAGGCGAFLTEYDHLLKDDPEWSERAAAF
CCCCCCCCHHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHH
VQKLKDFSSVLVELDFHQMDLALETPQVVTYQDSCHLRNVMHTSLEPRQLLKSIKGAEFK
HHHHHHHHHHHHHHCHHHHHHEECCCCEEEECCCHHHHHHHHCCCCHHHHHHHHCCCCHH
EMEKADSCCGSAGIYNIVEVEMSMKILDSKMAAVKATEAILIVTANPGCLLQMKLGIERE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCC
GLSGKVRAVHLADLLLEAAGHKTS
CCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377