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The map label for this gene is glcD

Identifier: 16079920

GI number: 16079920

Start: 2933185

End: 2934597

Strand: Direct

Name: glcD

Synonym: BSU28680

Alternate gene names: 16079920

Gene position: 2933185-2934597 (Clockwise)

Preceding gene: 255767659

Following gene: 16079921

Centisome position: 69.58

GC content: 47.91

Gene sequence:

>1413_bases
ATGATCACCAAGGATGTAAAGGAGCAGCTGATTCAGGTCTCCGGACCAGAAAACTTTGATGACTCGAATGCCGGCCGTCT
CGTCTATTCATATGATGCGACGCCTCAGTATCAGTCTATGCCTGATGCCGTGATTGCTCCGAGGAATACTGATGAAATAT
CACGCATTTTAACAATTTGCAGCGAACATCGAGTGCCGATCGTTCCAAGAGGATCAGGGACAAACCTGTGCGGCGGCACG
TGCCCGACTGAAGGCGGACTTGTGCTTCTCTTTAAGCACATGAACCAGATTCTTGAAATTGATGAAGAAAATTTAACTGC
CACAGTTCAGCCGGGCGTCATTACGTTGGATATGATCCGGGCAGTTGAAAGCAAAGGTTTATTTTATCCGCCTGACCCGA
GTTCTATGAAAATATCAACTATCGGGGGCAACATCAATGAAAATTCCGGCGGGCTACGCGGATTAAAATACGGTGTCACC
CGCGACTATGTTATTGGACTGGAGGTTGTTCTCGCAAATGGTGACATCATCCGCACCGGCGGAAAACTCGCAAAGGATGT
GGCCGGATACGATCTCACACGTCTTTTTGTCGGCTCAGAAGGAACACTCGGCATTGTGACAGAGGCAATCGTCAAACTCG
TTCCAAAGCCGGAAACAAAGAAGACGCTGCTTGCCCTTTATGAAAACATCGATGCCGCTGCTCAAACGGTATCGGACATT
ATTGCCGCAAAAATTATACCCGCTACGCTTGAATTTCTCGATCAGCCTACACTATTGGTGATTGAGGATTATGCCAAAAT
CGGACTTCCGACAAGCGCAAAAGCAGTTCTGTTAATTGAACAAGACGGGCCATTTGAAACTGTTGAACGGGATATGGAAA
AGATTGAGGCGATCTGCAAAAAAGGCGACGCCGTATCCGTTCAGACCGCACAAACAGAGGAGGAAGCGTTTGCCCTGACT
GAAGCCCGGCGTTCTGCACTTTCTGCCCTAGCCCGCCTCAAACCGACGACGATTTTAGAGGATGCGACCGTTCCTCGCTC
AGAAATCGCCAATATGGTTAAAGCGATTAATGATATTGCTGCTAAATATGATATTTCCATCTGCACCTTTGGTCATGCGG
GAGACGGGAACCTTCACCCGACATGCACGACAGACATAAGAAATAAGGATGAAATGGAACGTGTCGAACAGGCTTTTGCC
GAGATTTTTGAGAAAGCGATTGAACTTGGCGGGACGATAACCGGAGAGCACGGAGTCGGGGAAATGAAAGCGCCTTACTT
GGAAATGAAGCTGAAAAAAGAAGGCATTGATGCGATGAAAGCGTTGAAAGCGGCATTTGACCCCCGGAACATATTAAATC
CCGGCAAAATGTTTGCGAAAGATGCCAGAAAACGTGTGGTGGCGGAAAGATGA

Upstream 100 bases:

>100_bases
AGAGTTTCAGTTTCATTTTTTGTATGTTTCTCCATGGAAGGCTTTGCTTTTTTTCTTTTACAATAGATTGAAAGCGTTTT
TTGACAGGGGGATGGGTGAA

Downstream 100 bases:

>100_bases
CAACAGAAAAAGAAATGAAACAGATCCAAAATGAATTTAAAGAGCGTATGGATGAAGGAGAGTTATTAAATTGTATGCGC
TGCGGCTTCTGTCTTCCTTC

Product: glycolate oxidase subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MITKDVKEQLIQVSGPENFDDSNAGRLVYSYDATPQYQSMPDAVIAPRNTDEISRILTICSEHRVPIVPRGSGTNLCGGT
CPTEGGLVLLFKHMNQILEIDEENLTATVQPGVITLDMIRAVESKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVT
RDYVIGLEVVLANGDIIRTGGKLAKDVAGYDLTRLFVGSEGTLGIVTEAIVKLVPKPETKKTLLALYENIDAAAQTVSDI
IAAKIIPATLEFLDQPTLLVIEDYAKIGLPTSAKAVLLIEQDGPFETVERDMEKIEAICKKGDAVSVQTAQTEEEAFALT
EARRSALSALARLKPTTILEDATVPRSEIANMVKAINDIAAKYDISICTFGHAGDGNLHPTCTTDIRNKDEMERVEQAFA
EIFEKAIELGGTITGEHGVGEMKAPYLEMKLKKEGIDAMKALKAAFDPRNILNPGKMFAKDARKRVVAER

Sequences:

>Translated_470_residues
MITKDVKEQLIQVSGPENFDDSNAGRLVYSYDATPQYQSMPDAVIAPRNTDEISRILTICSEHRVPIVPRGSGTNLCGGT
CPTEGGLVLLFKHMNQILEIDEENLTATVQPGVITLDMIRAVESKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVT
RDYVIGLEVVLANGDIIRTGGKLAKDVAGYDLTRLFVGSEGTLGIVTEAIVKLVPKPETKKTLLALYENIDAAAQTVSDI
IAAKIIPATLEFLDQPTLLVIEDYAKIGLPTSAKAVLLIEQDGPFETVERDMEKIEAICKKGDAVSVQTAQTEEEAFALT
EARRSALSALARLKPTTILEDATVPRSEIANMVKAINDIAAKYDISICTFGHAGDGNLHPTCTTDIRNKDEMERVEQAFA
EIFEKAIELGGTITGEHGVGEMKAPYLEMKLKKEGIDAMKALKAAFDPRNILNPGKMFAKDARKRVVAER
>Mature_470_residues
MITKDVKEQLIQVSGPENFDDSNAGRLVYSYDATPQYQSMPDAVIAPRNTDEISRILTICSEHRVPIVPRGSGTNLCGGT
CPTEGGLVLLFKHMNQILEIDEENLTATVQPGVITLDMIRAVESKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVT
RDYVIGLEVVLANGDIIRTGGKLAKDVAGYDLTRLFVGSEGTLGIVTEAIVKLVPKPETKKTLLALYENIDAAAQTVSDI
IAAKIIPATLEFLDQPTLLVIEDYAKIGLPTSAKAVLLIEQDGPFETVERDMEKIEAICKKGDAVSVQTAQTEEEAFALT
EARRSALSALARLKPTTILEDATVPRSEIANMVKAINDIAAKYDISICTFGHAGDGNLHPTCTTDIRNKDEMERVEQAFA
EIFEKAIELGGTITGEHGVGEMKAPYLEMKLKKEGIDAMKALKAAFDPRNILNPGKMFAKDARKRVVAER

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Homo sapiens, GI37595756, Length=463, Percent_Identity=32.1814254859611, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI37595754, Length=486, Percent_Identity=30.6584362139918, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI119964728, Length=425, Percent_Identity=28.7058823529412, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI4501993, Length=222, Percent_Identity=30.1801801801802, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1789351, Length=428, Percent_Identity=39.2523364485981, Blast_Score=324, Evalue=9e-90,
Organism=Escherichia coli, GI1787977, Length=513, Percent_Identity=26.3157894736842, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI48994907, Length=441, Percent_Identity=25.1700680272109, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI48994914, Length=161, Percent_Identity=33.5403726708075, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71992373, Length=455, Percent_Identity=31.6483516483516, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI71992381, Length=455, Percent_Identity=30.989010989011, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI71992392, Length=365, Percent_Identity=32.8767123287671, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17534361, Length=439, Percent_Identity=29.1571753986333, Blast_Score=185, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17556096, Length=466, Percent_Identity=24.2489270386266, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320027, Length=474, Percent_Identity=32.2784810126582, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6320023, Length=451, Percent_Identity=28.8248337028825, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6320764, Length=454, Percent_Identity=27.9735682819383, Blast_Score=180, Evalue=4e-46,
Organism=Drosophila melanogaster, GI18921117, Length=428, Percent_Identity=28.2710280373832, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24639277, Length=428, Percent_Identity=28.2710280373832, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24639275, Length=428, Percent_Identity=28.2710280373832, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24653753, Length=473, Percent_Identity=23.0443974630021, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLCD_BACSU (P94535)

Other databases:

- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   D69984
- RefSeq:   NP_390746.1
- ProteinModelPortal:   P94535
- SMR:   P94535
- EnsemblBacteria:   EBBACT00000001218
- GeneID:   937770
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28680
- NMPDR:   fig|224308.1.peg.2871
- GenoList:   BSU28680
- GeneTree:   EBGT00050000001203
- HOGENOM:   HBG553036
- OMA:   SICTFGH
- PhylomeDB:   P94535
- ProtClustDB:   CLSK873408
- BioCyc:   BSUB:BSU28680-MONOMER
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.30.465.20
- Gene3D:   G3DSA:1.10.45.10
- TIGRFAMs:   TIGR00387

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4; SSF55103 FAD-binding_2; SSF56176 FAD-binding_2

EC number: 1.1.3.15

Molecular weight: Translated: 50937; Mature: 50937

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS51387 FAD_PCMH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKDVKEQLIQVSGPENFDDSNAGRLVYSYDATPQYQSMPDAVIAPRNTDEISRILTIC
CCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHH
SEHRVPIVPRGSGTNLCGGTCPTEGGLVLLFKHMNQILEIDEENLTATVQPGVITLDMIR
HCCCCCEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEECCCEEHHHHHH
AVESKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVTRDYVIGLEVVLANGDIIRTG
HHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCCCEEEEEEEEEECCCEEECC
GKLAKDVAGYDLTRLFVGSEGTLGIVTEAIVKLVPKPETKKTLLALYENIDAAAQTVSDI
CHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IAAKIIPATLEFLDQPTLLVIEDYAKIGLPTSAKAVLLIEQDGPFETVERDMEKIEAICK
HHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
KGDAVSVQTAQTEEEAFALTEARRSALSALARLKPTTILEDATVPRSEIANMVKAINDIA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHH
AKYDISICTFGHAGDGNLHPTCTTDIRNKDEMERVEQAFAEIFEKAIELGGTITGEHGVG
HHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
EMKAPYLEMKLKKEGIDAMKALKAAFDPRNILNPGKMFAKDARKRVVAER
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITKDVKEQLIQVSGPENFDDSNAGRLVYSYDATPQYQSMPDAVIAPRNTDEISRILTIC
CCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHH
SEHRVPIVPRGSGTNLCGGTCPTEGGLVLLFKHMNQILEIDEENLTATVQPGVITLDMIR
HCCCCCEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEECCCEEHHHHHH
AVESKGLFYPPDPSSMKISTIGGNINENSGGLRGLKYGVTRDYVIGLEVVLANGDIIRTG
HHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCCCEEEEEEEEEECCCEEECC
GKLAKDVAGYDLTRLFVGSEGTLGIVTEAIVKLVPKPETKKTLLALYENIDAAAQTVSDI
CHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IAAKIIPATLEFLDQPTLLVIEDYAKIGLPTSAKAVLLIEQDGPFETVERDMEKIEAICK
HHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
KGDAVSVQTAQTEEEAFALTEARRSALSALARLKPTTILEDATVPRSEIANMVKAINDIA
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHH
AKYDISICTFGHAGDGNLHPTCTTDIRNKDEMERVEQAFAEIFEKAIELGGTITGEHGVG
HHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
EMKAPYLEMKLKKEGIDAMKALKAAFDPRNILNPGKMFAKDARKRVVAER
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377