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The map label for this gene is leuC

Identifier: 16079878

GI number: 16079878

Start: 2889552

End: 2890970

Strand: Reverse

Name: leuC

Synonym: BSU28260

Alternate gene names: 16079878

Gene position: 2890970-2889552 (Counterclockwise)

Preceding gene: 255767650

Following gene: 16079877

Centisome position: 68.58

GC content: 47.22

Gene sequence:

>1419_bases
ATGATGCCTCGAACAATCATCGAAAAGATTTGGGATCAGCATATTGTAAAACATGGTGAGGGAAAGCCGGATCTTCTCTA
TATTGATTTGCACCTCATTCATGAGGTGACGTCTCCTCAGGCATTTGAAGGCTTGAGACAAAAGGGAAGAAAGGTCAGAA
GACCCCAAAACACATTTGCGACAATGGACCACAACATCCCGACTGTCAATCGTTTTGAGATAAAGGATGAAGTTGCGAAA
CGCCAGGTAACGGCGCTTGAAAGAAACTGTGAGGAATTTGGCGTGCGCCTTGCCGATCTTCACAGTGTGGATCAAGGGAT
TGTCCATGTCGTCGGACCTGAACTAGGCTTAACGCTTCCAGGGAAAACGATTGTGTGCGGTGACAGTCATACATCAACAC
ATGGCGCTTTCGGCGCTCTTGCATTTGGAATCGGGACGAGTGAAGTTGAACATGTCCTTTCCACACAGACACTTTGGCAG
CAGCGTCCAAAAACACTTGAAGTGCGCGTAGATGGAACGCTTCAAAAAGGGGTAACGGCAAAGGATGTCATCCTTGCTGT
CATCGGCAAATACGGTGTGAAATTCGGCACAGGCTACGTCATTGAATACACTGGGGAAGTATTCAGAAATATGACGATGG
ATGAACGAATGACTGTTTGTAACATGTCAATTGAAGCAGGAGCAAGAGCAGGTTTGATTGCACCTGACGAGGTGACGTTT
GAATATTGCAAAAATCGCAAGTACACGCCAAAAGGCGAAGAATTTGACAAGGCCGTAGAGGAATGGAAGGCGCTGCGCAC
AGACCCGGGCGCTGTTTACGATAAATCTATCGTCCTTGACGGCAACAAAATTTCCCCTATGGTGACATGGGGCATTAACC
CGGGAATGGTTCTTCCTGTCGATTCTGAAGTTCCTGCGCCGGAAAGCTTTTCTGCAGAAGACGATAAAAAAGAAGCGATT
CGTGCTTATGAATATATGGGATTGACTCCTCACCAAAAAATTGAAGACATTAAAGTGGAGCACGTATTTATCGGTTCCTG
CACAAATTCCCGCATGACTGACCTTCGACAGGCTGCTGACATGATCAAAGGGAAGAAGGTAGCTGACAGCGTAAGGGCCA
TCGTCGTGCCCGGATCCCAAAGCGTGAAGCTTCAGGCTGAAAAAGAAGGGCTTGACCAGATTTTCTTGGAAGCTGGATTT
GAATGGAGAGAGTCAGGCTGCAGCATGTGTTTGAGTATGAATAATGATGTTGTTCCTGAGGGAGAGCGCTGTGCATCAAC
CTCTAACCGCAACTTCGAGGGCAGACAAGGAAAAGGTGCAAGAACACATCTCGTCAGCCCGGCAATGGCTGCGATGGCTG
CCATTCACGGACACTTCGTTGATGTCAGAAAGTTTTATCAGGAAAAAACAGTTGTGTAA

Upstream 100 bases:

>100_bases
GAGGAAGTTAAGGCAGCAATCATGAGTGAAAATACAATTTCTAATGTGTGACAGCTTACGTTAAGCGGTCTTAGCTCTAG
GTAGAGGGAGGAAATAAAAG

Downstream 100 bases:

>100_bases
GGAGTGCGCGAGATGGAACCTTTGAAATCACATACGGGGAAAGCAGCCGTATTAAATCGGATCAATGTGGATACAGACCA
GATTATTCCTAAGCAATTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MMPRTIIEKIWDQHIVKHGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFATMDHNIPTVNRFEIKDEVAK
RQVTALERNCEEFGVRLADLHSVDQGIVHVVGPELGLTLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQ
QRPKTLEVRVDGTLQKGVTAKDVILAVIGKYGVKFGTGYVIEYTGEVFRNMTMDERMTVCNMSIEAGARAGLIAPDEVTF
EYCKNRKYTPKGEEFDKAVEEWKALRTDPGAVYDKSIVLDGNKISPMVTWGINPGMVLPVDSEVPAPESFSAEDDKKEAI
RAYEYMGLTPHQKIEDIKVEHVFIGSCTNSRMTDLRQAADMIKGKKVADSVRAIVVPGSQSVKLQAEKEGLDQIFLEAGF
EWRESGCSMCLSMNNDVVPEGERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGHFVDVRKFYQEKTVV

Sequences:

>Translated_472_residues
MMPRTIIEKIWDQHIVKHGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFATMDHNIPTVNRFEIKDEVAK
RQVTALERNCEEFGVRLADLHSVDQGIVHVVGPELGLTLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQ
QRPKTLEVRVDGTLQKGVTAKDVILAVIGKYGVKFGTGYVIEYTGEVFRNMTMDERMTVCNMSIEAGARAGLIAPDEVTF
EYCKNRKYTPKGEEFDKAVEEWKALRTDPGAVYDKSIVLDGNKISPMVTWGINPGMVLPVDSEVPAPESFSAEDDKKEAI
RAYEYMGLTPHQKIEDIKVEHVFIGSCTNSRMTDLRQAADMIKGKKVADSVRAIVVPGSQSVKLQAEKEGLDQIFLEAGF
EWRESGCSMCLSMNNDVVPEGERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGHFVDVRKFYQEKTVV
>Mature_472_residues
MMPRTIIEKIWDQHIVKHGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFATMDHNIPTVNRFEIKDEVAK
RQVTALERNCEEFGVRLADLHSVDQGIVHVVGPELGLTLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQ
QRPKTLEVRVDGTLQKGVTAKDVILAVIGKYGVKFGTGYVIEYTGEVFRNMTMDERMTVCNMSIEAGARAGLIAPDEVTF
EYCKNRKYTPKGEEFDKAVEEWKALRTDPGAVYDKSIVLDGNKISPMVTWGINPGMVLPVDSEVPAPESFSAEDDKKEAI
RAYEYMGLTPHQKIEDIKVEHVFIGSCTNSRMTDLRQAADMIKGKKVADSVRAIVVPGSQSVKLQAEKEGLDQIFLEAGF
EWRESGCSMCLSMNNDVVPEGERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGHFVDVRKFYQEKTVV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=365, Percent_Identity=28.2191780821918, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI41352693, Length=462, Percent_Identity=24.4588744588745, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=60.2150537634409, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1787531, Length=452, Percent_Identity=26.5486725663717, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87081781, Length=342, Percent_Identity=26.0233918128655, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI2367097, Length=349, Percent_Identity=23.7822349570201, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=363, Percent_Identity=28.6501377410468, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32564738, Length=363, Percent_Identity=28.6501377410468, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=310, Percent_Identity=28.7096774193548, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=454, Percent_Identity=25.1101321585903, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=56.2632696390658, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6320440, Length=467, Percent_Identity=28.051391862955, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322261, Length=383, Percent_Identity=29.7650130548303, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=365, Percent_Identity=27.3972602739726, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=26.7015706806283, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281365315, Length=382, Percent_Identity=26.7015706806283, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17864292, Length=382, Percent_Identity=26.7015706806283, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28571643, Length=363, Percent_Identity=25.8953168044077, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 52393; Mature: 52393

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00450 ACONITASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPRTIIEKIWDQHIVKHGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFA
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHE
TMDHNIPTVNRFEIKDEVAKRQVTALERNCEEFGVRLADLHSVDQGIVHVVGPELGLTLP
EECCCCCCCEEEEHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHCCCCEEEECCCCCEECC
GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQQRPKTLEVRVDGTLQKGVTA
CCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCH
KDVILAVIGKYGVKFGTGYVIEYTGEVFRNMTMDERMTVCNMSIEAGARAGLIAPDEVTF
HHHHHHHHHHHCCEECCCEEEEECHHHHHCCCHHHHHEEEECCHHCCCCCCCCCCCHHHH
EYCKNRKYTPKGEEFDKAVEEWKALRTDPGAVYDKSIVLDGNKISPMVTWGINPGMVLPV
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCEEEEECCCCCCEEEECCCCCEEEEE
DSEVPAPESFSAEDDKKEAIRAYEYMGLTPHQKIEDIKVEHVFIGSCTNSRMTDLRQAAD
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHEEEEEEEEECCCCHHHHHHHHHHH
MIKGKKVADSVRAIVVPGSQSVKLQAEKEGLDQIFLEAGFEWRESGCSMCLSMNNDVVPE
HHCCHHHHCCCEEEEECCCCCEEEEEHHCCHHHHHHHCCCHHHHCCCCEEEECCCCCCCC
GERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGHFVDVRKFYQEKTVV
CHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMPRTIIEKIWDQHIVKHGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKGRKVRRPQNTFA
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHE
TMDHNIPTVNRFEIKDEVAKRQVTALERNCEEFGVRLADLHSVDQGIVHVVGPELGLTLP
EECCCCCCCEEEEHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHCCCCEEEECCCCCEECC
GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQQRPKTLEVRVDGTLQKGVTA
CCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCH
KDVILAVIGKYGVKFGTGYVIEYTGEVFRNMTMDERMTVCNMSIEAGARAGLIAPDEVTF
HHHHHHHHHHHCCEECCCEEEEECHHHHHCCCHHHHHEEEECCHHCCCCCCCCCCCHHHH
EYCKNRKYTPKGEEFDKAVEEWKALRTDPGAVYDKSIVLDGNKISPMVTWGINPGMVLPV
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCEEEEECCCCCCEEEECCCCCEEEEE
DSEVPAPESFSAEDDKKEAIRAYEYMGLTPHQKIEDIKVEHVFIGSCTNSRMTDLRQAAD
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHEEEEEEEEECCCCHHHHHHHHHHH
MIKGKKVADSVRAIVVPGSQSVKLQAEKEGLDQIFLEAGFEWRESGCSMCLSMNNDVVPE
HHCCHHHHCCCEEEEECCCCCEEEEEHHCCHHHHHHHCCCHHHHCCCCEEEECCCCCCCC
GERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGHFVDVRKFYQEKTVV
CHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA