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The map label for this gene is leuD

Identifier: 16079877

GI number: 16079877

Start: 2888940

End: 2889539

Strand: Reverse

Name: leuD

Synonym: BSU28250

Alternate gene names: 16079877

Gene position: 2889539-2888940 (Counterclockwise)

Preceding gene: 16079878

Following gene: 255767649

Centisome position: 68.54

GC content: 41.67

Gene sequence:

>600_bases
ATGGAACCTTTGAAATCACATACGGGGAAAGCAGCCGTATTAAATCGGATCAATGTGGATACAGACCAGATTATTCCTAA
GCAATTTTTGAAGAGGATTGAAAGAACAGGCTACGGACGTTTTGCATTCTTTGACTGGAGATATGATGCGAATGGTGAAC
CGAACCCTGAATTTGAATTAAACCAGCCTGTTTATCAAGGAGCTTCCATTTTAATAGCAGGAGAAAACTTCGGCTGCGGG
TCATCGCGTGAACACGCTCCGTGGGCACTTGATGATTATGGGTTTAAAATTATCATTGCGCCGTCATTCGCTGATATTTT
CCATCAGAACTGCTTTAAAAACGGCATGCTTCCGATCCGCATGCCATATGACAATTGGAAACAGCTTGTCGGCCAGTATG
AAAACCAGTCATTGCAAATGACTGTTGACCTTGAAAATCAGCTGATTCATGACAGTGAAGGCAATCAAATTTCATTTGAA
GTTGATCCGCATTGGAAAGAGATGCTGATCAACGGATATGATGAAATTTCATTAACGCTGCTGCTGGAAGATGAAATCAA
GCAATTTGAATCACAAAGAAGCTCTTGGCTTCAAGCCTGA

Upstream 100 bases:

>100_bases
CGTCAGCCCGGCAATGGCTGCGATGGCTGCCATTCACGGACACTTCGTTGATGTCAGAAAGTTTTATCAGGAAAAAACAG
TTGTGTAAGGAGTGCGCGAG

Downstream 100 bases:

>100_bases
AAAAGCGGCCGGTATTTGTCCGGCGGCTTTTTTTGCCTGGTGAAACCTTTCATTTCTTGTTTTTGTTCACAGGTGTTGAT
ACACTTGAACAAAATAAATG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MEPLKSHTGKAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYDANGEPNPEFELNQPVYQGASILIAGENFGCG
SSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIRMPYDNWKQLVGQYENQSLQMTVDLENQLIHDSEGNQISFE
VDPHWKEMLINGYDEISLTLLLEDEIKQFESQRSSWLQA

Sequences:

>Translated_199_residues
MEPLKSHTGKAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYDANGEPNPEFELNQPVYQGASILIAGENFGCG
SSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIRMPYDNWKQLVGQYENQSLQMTVDLENQLIHDSEGNQISFE
VDPHWKEMLINGYDEISLTLLLEDEIKQFESQRSSWLQA
>Mature_199_residues
MEPLKSHTGKAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYDANGEPNPEFELNQPVYQGASILIAGENFGCG
SSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIRMPYDNWKQLVGQYENQSLQMTVDLENQLIHDSEGNQISFE
VDPHWKEMLINGYDEISLTLLLEDEIKQFESQRSSWLQA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=199, Percent_Identity=48.7437185929648, Blast_Score=189, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=47.2361809045226, Blast_Score=177, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23022; Mature: 23022

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPLKSHTGKAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYDANGEPNPEFEL
CCCCHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEE
NQPVYQGASILIAGENFGCGSSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIR
CCCHHCCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCEEEE
MPYDNWKQLVGQYENQSLQMTVDLENQLIHDSEGNQISFEVDPHWKEMLINGYDEISLTL
ECHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCCEEEEEECCCHHHHHHCCCCCEEEEE
LLEDEIKQFESQRSSWLQA
EEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEPLKSHTGKAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYDANGEPNPEFEL
CCCCHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEE
NQPVYQGASILIAGENFGCGSSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIR
CCCHHCCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCEEEE
MPYDNWKQLVGQYENQSLQMTVDLENQLIHDSEGNQISFEVDPHWKEMLINGYDEISLTL
ECHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCCEEEEEECCCHHHHHHCCCCCEEEEE
LLEDEIKQFESQRSSWLQA
EEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA