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The map label for this gene is leuB

Identifier: 255767650

GI number: 255767650

Start: 2891020

End: 2892117

Strand: Reverse

Name: leuB

Synonym: BSU28270

Alternate gene names: 255767650

Gene position: 2892117-2891020 (Counterclockwise)

Preceding gene: 16079880

Following gene: 16079878

Centisome position: 68.61

GC content: 46.63

Gene sequence:

>1098_bases
TTGAAGAAACGTATTGCTCTATTGCCCGGAGACGGGATCGGCCCTGAAGTATTAGAATCAGCGACAGACGTACTGAAAAG
TGTTGCCGAACGCTTTAACCATGAATTTGAATTTGAATATGGCCTGATTGGAGGGGCGGCTATTGATGAACATCATAACC
CCCTCCCGGAGGAAACCGTTGCTGCTTGTAAAAATGCAGACGCGATATTGCTTGGTGCTGTCGGCGGACCGAAATGGGAT
CAAAATCCTTCGGAACTGAGACCGGAAAAAGGGCTGCTCAGCATCAGAAAACAGCTTGATTTGTTTGCGAATTTGCGGCC
TGTGAAGGTATTTGAAAGCCTTTCTGACGCTTCGCCTTTGAAAAAAGAATATATAGATAATGTTGATTTCGTTATCGTTC
GTGAGCTCACAGGCGGCTTGTATTTCGGCCAGCCGAGCAAACGTTATGTAAACACTGAAGGTGAGCAGGAAGCAGTAGAT
ACACTGTTTTATAAGCGAACGGAAATTGAACGAGTGATCAGAGAGGGCTTCAAAATGGCGGCAGCCAGAAAAGGCAAAGT
GACCTCTGTAGATAAAGCGAATGTTCTTGAATCAAGCCGGCTGTGGCGTGAAGTGGCTGAGGACGTTGCACAAGAATTTC
CTGATGTGAAGCTTGAGCACATGCTTGTGGATAACGCGGCCATGCAGCTAATTTATGCACCGAATCAATTTGATGTCGTG
GTGACTGAAAATATGTTCGGTGATATTTTAAGCGATGAAGCGTCCATGCTTACAGGCTCGCTCGGAATGCTCCCGTCAGC
CAGCCTATCAAGCTCTGGCCTTCATCTGTTTGAACCTGTTCATGGCTCCGCGCCTGATATTGCCGGTAAAGGGATGGCAA
ATCCGTTCGCAGCCATATTGTCAGCGGCAATGCTTTTGAGAACATCTTTCGGGCTTGAAGAGGAAGCGAAAGCTGTAGAA
GATGCGGTAAACAAAGTCTTGGCTTCTGGAAAAAGAACAAGAGACTTGGCACGGAGTGAAGAGTTCAGCAGCACTCAGGC
CATTACAGAGGAAGTTAAGGCAGCAATCATGAGTGAAAATACAATTTCTAATGTGTGA

Upstream 100 bases:

>100_bases
ATTTGAACGCAGTAAACCGTCAATTGGTTTTCCAGTCGAATATGAGCGGATTGAAAAACCACACAGCTGTCGGATCATAA
AAGAAAGGAGAACGGTTAAC

Downstream 100 bases:

>100_bases
CAGCTTACGTTAAGCGGTCTTAGCTCTAGGTAGAGGGAGGAAATAAAAGATGATGCCTCGAACAATCATCGAAAAGATTT
GGGATCAGCATATTGTAAAA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKKRIALLPGDGIGPEVLESATDVLKSVAERFNHEFEFEYGLIGGAAIDEHHNPLPEETVAACKNADAILLGAVGGPKWD
QNPSELRPEKGLLSIRKQLDLFANLRPVKVFESLSDASPLKKEYIDNVDFVIVRELTGGLYFGQPSKRYVNTEGEQEAVD
TLFYKRTEIERVIREGFKMAAARKGKVTSVDKANVLESSRLWREVAEDVAQEFPDVKLEHMLVDNAAMQLIYAPNQFDVV
VTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAILSAAMLLRTSFGLEEEAKAVE
DAVNKVLASGKRTRDLARSEEFSSTQAITEEVKAAIMSENTISNV

Sequences:

>Translated_365_residues
MKKRIALLPGDGIGPEVLESATDVLKSVAERFNHEFEFEYGLIGGAAIDEHHNPLPEETVAACKNADAILLGAVGGPKWD
QNPSELRPEKGLLSIRKQLDLFANLRPVKVFESLSDASPLKKEYIDNVDFVIVRELTGGLYFGQPSKRYVNTEGEQEAVD
TLFYKRTEIERVIREGFKMAAARKGKVTSVDKANVLESSRLWREVAEDVAQEFPDVKLEHMLVDNAAMQLIYAPNQFDVV
VTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAILSAAMLLRTSFGLEEEAKAVE
DAVNKVLASGKRTRDLARSEEFSSTQAITEEVKAAIMSENTISNV
>Mature_365_residues
MKKRIALLPGDGIGPEVLESATDVLKSVAERFNHEFEFEYGLIGGAAIDEHHNPLPEETVAACKNADAILLGAVGGPKWD
QNPSELRPEKGLLSIRKQLDLFANLRPVKVFESLSDASPLKKEYIDNVDFVIVRELTGGLYFGQPSKRYVNTEGEQEAVD
TLFYKRTEIERVIREGFKMAAARKGKVTSVDKANVLESSRLWREVAEDVAQEFPDVKLEHMLVDNAAMQLIYAPNQFDVV
VTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAILSAAMLLRTSFGLEEEAKAVE
DAVNKVLASGKRTRDLARSEEFSSTQAITEEVKAAIMSENTISNV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=355, Percent_Identity=33.2394366197183, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI28178816, Length=360, Percent_Identity=30.2777777777778, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI28178821, Length=360, Percent_Identity=30.5555555555556, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4758582, Length=326, Percent_Identity=29.1411042944785, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178838, Length=326, Percent_Identity=28.8343558282209, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI28178819, Length=218, Percent_Identity=33.0275229357798, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI87081683, Length=344, Percent_Identity=54.0697674418605, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1788101, Length=342, Percent_Identity=36.5497076023392, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1787381, Length=276, Percent_Identity=27.1739130434783, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=368, Percent_Identity=31.25, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17550882, Length=343, Percent_Identity=32.0699708454811, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI25144293, Length=329, Percent_Identity=30.3951367781155, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=371, Percent_Identity=28.8409703504043, Blast_Score=124, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=49.5867768595041, Blast_Score=321, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6322097, Length=363, Percent_Identity=34.9862258953168, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6324709, Length=359, Percent_Identity=33.7047353760446, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324291, Length=356, Percent_Identity=33.1460674157303, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24643270, Length=353, Percent_Identity=32.8611898016997, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24643268, Length=353, Percent_Identity=32.8611898016997, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI281362242, Length=357, Percent_Identity=32.2128851540616, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24648872, Length=357, Percent_Identity=32.2128851540616, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24661184, Length=361, Percent_Identity=30.4709141274238, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078633, Length=358, Percent_Identity=26.536312849162, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24650122, Length=358, Percent_Identity=26.536312849162, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI161078635, Length=358, Percent_Identity=26.536312849162, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161078637, Length=358, Percent_Identity=26.536312849162, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI161078639, Length=353, Percent_Identity=26.628895184136, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20130355, Length=352, Percent_Identity=23.5795454545455, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_BACSU (P05645)

Other databases:

- EMBL:   Y00353
- EMBL:   Z75208
- EMBL:   AL009126
- PIR:   A26522
- RefSeq:   NP_390705.2
- ProteinModelPortal:   P05645
- SMR:   P05645
- EnsemblBacteria:   EBBACT00000001889
- GeneID:   936221
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28270
- NMPDR:   fig|224308.1.peg.2830
- GenoList:   BSU28270
- GeneTree:   EBGT00050000000661
- HOGENOM:   HBG518924
- PhylomeDB:   P05645
- ProtClustDB:   PRK00772
- BioCyc:   BSUB:BSU28270-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39845; Mature: 39845

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 96-96 BINDING 106-106 BINDING 134-134 BINDING 224-224

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRIALLPGDGIGPEVLESATDVLKSVAERFNHEFEFEYGLIGGAAIDEHHNPLPEETV
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECEECCCEECCCCCCCCHHHH
AACKNADAILLGAVGGPKWDQNPSELRPEKGLLSIRKQLDLFANLRPVKVFESLSDASPL
HHHCCCCEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
KKEYIDNVDFVIVRELTGGLYFGQPSKRYVNTEGEQEAVDTLFYKRTEIERVIREGFKMA
HHHHHCCCCEEEEEEHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AARKGKVTSVDKANVLESSRLWREVAEDVAQEFPDVKLEHMLVDNAAMQLIYAPNQFDVV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCEEEE
VTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAIL
EECHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHH
SAAMLLRTSFGLEEEAKAVEDAVNKVLASGKRTRDLARSEEFSSTQAITEEVKAAIMSEN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TISNV
HHCCC
>Mature Secondary Structure
MKKRIALLPGDGIGPEVLESATDVLKSVAERFNHEFEFEYGLIGGAAIDEHHNPLPEETV
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECEECCCEECCCCCCCCHHHH
AACKNADAILLGAVGGPKWDQNPSELRPEKGLLSIRKQLDLFANLRPVKVFESLSDASPL
HHHCCCCEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
KKEYIDNVDFVIVRELTGGLYFGQPSKRYVNTEGEQEAVDTLFYKRTEIERVIREGFKMA
HHHHHCCCCEEEEEEHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AARKGKVTSVDKANVLESSRLWREVAEDVAQEFPDVKLEHMLVDNAAMQLIYAPNQFDVV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCEEEE
VTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAIL
EECHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHH
SAAMLLRTSFGLEEEAKAVEDAVNKVLASGKRTRDLARSEEFSSTQAITEEVKAAIMSEN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TISNV
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3110742; 8969504; 9384377