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The map label for this gene is hemC

Identifier: 16079867

GI number: 16079867

Start: 2875951

End: 2876895

Strand: Reverse

Name: hemC

Synonym: BSU28150

Alternate gene names: 16079867

Gene position: 2876895-2875951 (Counterclockwise)

Preceding gene: 255767646

Following gene: 16079866

Centisome position: 68.24

GC content: 45.71

Gene sequence:

>945_bases
ATGATGAGAACGATTAAAGTAGGTTCCAGACGGAGCAAACTCGCTATGACTCAAACAAAATGGGTTATTCAAAAACTGAA
GGAAATCAATCCTTCGTTTGCTTTTGAAATTAAAGAGATCGTGACAAAGGGCGACCGGATTGTCGATGTTACACTCTCAA
AAGTGGGTGGAAAAGGGCTTTTTGTCAAAGAAATTGAACAGGCGCTTTTAAACGAAGAGATTGATATGGCAGTGCACAGC
ATGAAGGACATGCCTGCTGTTTTGCCTGAAGGCCTTGTGATCGGCTGTATTCCTGAACGGGAGGACCCGCGTGATGCCCT
TATTTCAAAGAATCGCGTAAAGCTTTCAGAAATGAAGAAAGGTGCTGTCATTGGCACAAGCAGTTTAAGAAGAAGCGCGC
AGCTTTTGATTGAGCGCCCTGACCTTACAATTAAATGGATTAGAGGTAATATAGATACAAGACTTCAAAAGCTGGAAACA
GAGGATTATGACGCAATTATTTTAGCGGCTGCCGGCCTTTCCAGAATGGGTTGGAAGCAAGATGTCGTAACCGAATTCCT
TGAGCCTGAGCGCTGTTTGCCTGCTGTGGGGCAGGGAGCCCTGGCGATTGAGTGCCGAGAATCGGATGAAGAGCTGTTGG
CGTTGTTTTCTCAGTTTACAGATGAATATACAAAACGGACTGTCTTAGCGGAACGTGCTTTTTTAAACGCGATGGAGGGC
GGCTGCCAGGTTCCGATCGCGGGCTACTCCGTGTTAAATGGACAGGATGAAATTGAAATGACAGGTCTTGTCGCTTCACC
TGACGGCAAAATCATTTTTAAAGAAACCGTCACCGGAAACGATCCGGAGGAAGTAGGAAAGCGCTGTGCCGCTCTTATGG
CTGACAAAGGAGCAAAAGATTTAATTGATCGTGTAAAACGGGAGCTTGACGAGGATGGAAAATGA

Upstream 100 bases:

>100_bases
GTTTTCTGGTGCTGATGATCAATTATTTCCTGCTTGGAAGCCTGTCGCAATTCCATTGGTTCAGTTAAACGATGTCCCAA
GCAGATTCGGGAGGAAAGAA

Downstream 100 bases:

>100_bases
TTTTCCGTTGAAAGGAAAAACAGTGCTTGTCACCCGGAATAAGGCACAGGCAGCATCATTTCAGCAAAAAGTGGAGGCGC
TTGGCGGTAAAGCGGTTTTA

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MMRTIKVGSRRSKLAMTQTKWVIQKLKEINPSFAFEIKEIVTKGDRIVDVTLSKVGGKGLFVKEIEQALLNEEIDMAVHS
MKDMPAVLPEGLVIGCIPEREDPRDALISKNRVKLSEMKKGAVIGTSSLRRSAQLLIERPDLTIKWIRGNIDTRLQKLET
EDYDAIILAAAGLSRMGWKQDVVTEFLEPERCLPAVGQGALAIECRESDEELLALFSQFTDEYTKRTVLAERAFLNAMEG
GCQVPIAGYSVLNGQDEIEMTGLVASPDGKIIFKETVTGNDPEEVGKRCAALMADKGAKDLIDRVKRELDEDGK

Sequences:

>Translated_314_residues
MMRTIKVGSRRSKLAMTQTKWVIQKLKEINPSFAFEIKEIVTKGDRIVDVTLSKVGGKGLFVKEIEQALLNEEIDMAVHS
MKDMPAVLPEGLVIGCIPEREDPRDALISKNRVKLSEMKKGAVIGTSSLRRSAQLLIERPDLTIKWIRGNIDTRLQKLET
EDYDAIILAAAGLSRMGWKQDVVTEFLEPERCLPAVGQGALAIECRESDEELLALFSQFTDEYTKRTVLAERAFLNAMEG
GCQVPIAGYSVLNGQDEIEMTGLVASPDGKIIFKETVTGNDPEEVGKRCAALMADKGAKDLIDRVKRELDEDGK
>Mature_314_residues
MMRTIKVGSRRSKLAMTQTKWVIQKLKEINPSFAFEIKEIVTKGDRIVDVTLSKVGGKGLFVKEIEQALLNEEIDMAVHS
MKDMPAVLPEGLVIGCIPEREDPRDALISKNRVKLSEMKKGAVIGTSSLRRSAQLLIERPDLTIKWIRGNIDTRLQKLET
EDYDAIILAAAGLSRMGWKQDVVTEFLEPERCLPAVGQGALAIECRESDEELLALFSQFTDEYTKRTVLAERAFLNAMEG
GCQVPIAGYSVLNGQDEIEMTGLVASPDGKIIFKETVTGNDPEEVGKRCAALMADKGAKDLIDRVKRELDEDGK

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=346, Percent_Identity=42.485549132948, Blast_Score=251, Evalue=9e-67,
Organism=Homo sapiens, GI66933009, Length=346, Percent_Identity=42.485549132948, Blast_Score=250, Evalue=1e-66,
Organism=Escherichia coli, GI48994974, Length=305, Percent_Identity=45.9016393442623, Blast_Score=254, Evalue=6e-69,
Organism=Saccharomyces cerevisiae, GI6319996, Length=319, Percent_Identity=36.9905956112853, Blast_Score=189, Evalue=6e-49,
Organism=Drosophila melanogaster, GI20130425, Length=282, Percent_Identity=46.8085106382979, Blast_Score=244, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_BACSU (P16616)

Other databases:

- EMBL:   M57676
- EMBL:   AL009126
- PIR:   C35252
- RefSeq:   NP_390693.1
- ProteinModelPortal:   P16616
- SMR:   P16616
- EnsemblBacteria:   EBBACT00000002797
- GeneID:   937488
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU28150
- NMPDR:   fig|224308.1.peg.2818
- GenoList:   BSU28150
- GeneTree:   EBGT00050000001150
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   BSUB:BSU28150-MONOMER
- BRENDA:   2.5.1.61
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34839; Mature: 34839

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRTIKVGSRRSKLAMTQTKWVIQKLKEINPSFAFEIKEIVTKGDRIVDVTLSKVGGKGL
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCC
FVKEIEQALLNEEIDMAVHSMKDMPAVLPEGLVIGCIPEREDPRDALISKNRVKLSEMKK
CHHHHHHHHHCHHHHHHHHHHHCCHHHCCCCCEEEECCCCCCCHHHHHCCCCCCHHHHCC
GAVIGTSSLRRSAQLLIERPDLTIKWIRGNIDTRLQKLETEDYDAIILAAAGLSRMGWKQ
CCEEEHHHHHHHHHHEEECCCCEEEEEECCHHHHHHHHCCCCCCEEEEEECCHHHCCCHH
DVVTEFLEPERCLPAVGQGALAIECRESDEELLALFSQFTDEYTKRTVLAERAFLNAMEG
HHHHHHHCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GCQVPIAGYSVLNGQDEIEMTGLVASPDGKIIFKETVTGNDPEEVGKRCAALMADKGAKD
CCCCCCCCEEEECCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHH
LIDRVKRELDEDGK
HHHHHHHHHCCCCC
>Mature Secondary Structure
MMRTIKVGSRRSKLAMTQTKWVIQKLKEINPSFAFEIKEIVTKGDRIVDVTLSKVGGKGL
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCC
FVKEIEQALLNEEIDMAVHSMKDMPAVLPEGLVIGCIPEREDPRDALISKNRVKLSEMKK
CHHHHHHHHHCHHHHHHHHHHHCCHHHCCCCCEEEECCCCCCCHHHHHCCCCCCHHHHCC
GAVIGTSSLRRSAQLLIERPDLTIKWIRGNIDTRLQKLETEDYDAIILAAAGLSRMGWKQ
CCEEEHHHHHHHHHHEEECCCCEEEEEECCHHHHHHHHCCCCCCEEEEEECCHHHCCCHH
DVVTEFLEPERCLPAVGQGALAIECRESDEELLALFSQFTDEYTKRTVLAERAFLNAMEG
HHHHHHHCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GCQVPIAGYSVLNGQDEIEMTGLVASPDGKIIFKETVTGNDPEEVGKRCAALMADKGAKD
CCCCCCCCEEEECCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHH
LIDRVKRELDEDGK
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2110138; 9384377