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The map label for this gene is levG

Identifier: 16079758

GI number: 16079758

Start: 2760233

End: 2761060

Strand: Reverse

Name: levG

Synonym: BSU27040

Alternate gene names: 16079758

Gene position: 2761060-2760233 (Counterclockwise)

Preceding gene: 16079759

Following gene: 16079757

Centisome position: 65.5

GC content: 44.32

Gene sequence:

>828_bases
ATGGAGAAAGAAAAACGATTAACGAAGAAAGAAATTTTCAGCATGTTTATTCGTTCAAATTTTTTACTCGGTTCCTTTAA
CTTCGAACGTGTGCAGGCAATGGGATATTGTTATGTCATGATACCGGCGATCAAAAAATTGTACGGTCCCGGAGCGAAAA
GAAACGAAGCCTTACAGCGGCATTTGGAATGGTTTAATACACATCCGTGGCTGACAGCGCCTATATTTGGCGTGACGGCA
GCCATGGAAGAAGAAATGGCGAACAATAAAGGAATTGACGGAAAAGCGATAAGCGGAATGAAAATCGGTTTGATGGGACC
AATAGCGGGCGTAGGCGATCCAATTTTTTGGGGAACGATTCGTCCTGTCTTAGCTGCGCTAGGAGCCTCCCTTGCTTTAG
GAGGAAACATTGCCGGTCCTTTGCTATTCTTTTTCTTGCTGAATGCCATAAGATTAAGCACAAAATATTACGGATTAAAG
TATGGCTATGTGAAAGGAATGGAGATTCTTCAGGATTTAGCGGGGAATCGCATTCAAAAGCTTACAGAGGGCGCTTCGAT
TCTCGGGTTATTTGTTATGGGGGCTCTCGTGTCCAAATGGACCACCATCAACATTCCAATCGTTGTATCCAGGATTAAGG
ATGAAAGCGGAAAAGTAGATGTTCAAACGGTACAAAACGTGCTAGATAGCATTATGCCGGGGGCGCTGCCTCTCGGATTA
ACGTTATTGGTGGCATGGATGCTTCGCAAAGGGGTGAATCCACTTCTCATTATTTGCGGCATCTTTGTCATCGGGATTCT
CGGGTATTGGGCTGGATTCTTAGCATAA

Upstream 100 bases:

>100_bases
CTCACGGAGCAGTCGCGGCCGCATCAGACAGTGGCAGTGTTGCTGTTTATGATGACGATGATGATGATCTTGATGCCTAA
ACGATGAGGGGGAAGAAAAA

Downstream 100 bases:

>100_bases
GGTGTGTGAAGGAAAGAGAGAGAAACTGCAGCAGTCTTTTCATATATAAATCATTCGGATAACAGTGAAACAGAGGAGAA
TTGCCAGAAACGGACCAGCT

Product: phosphotransferase system (PTS) fructose-specific enzyme IID component

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIID-Fru; PTS system fructose-specific EIID component; p30

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MEKEKRLTKKEIFSMFIRSNFLLGSFNFERVQAMGYCYVMIPAIKKLYGPGAKRNEALQRHLEWFNTHPWLTAPIFGVTA
AMEEEMANNKGIDGKAISGMKIGLMGPIAGVGDPIFWGTIRPVLAALGASLALGGNIAGPLLFFFLLNAIRLSTKYYGLK
YGYVKGMEILQDLAGNRIQKLTEGASILGLFVMGALVSKWTTINIPIVVSRIKDESGKVDVQTVQNVLDSIMPGALPLGL
TLLVAWMLRKGVNPLLIICGIFVIGILGYWAGFLA

Sequences:

>Translated_275_residues
MEKEKRLTKKEIFSMFIRSNFLLGSFNFERVQAMGYCYVMIPAIKKLYGPGAKRNEALQRHLEWFNTHPWLTAPIFGVTA
AMEEEMANNKGIDGKAISGMKIGLMGPIAGVGDPIFWGTIRPVLAALGASLALGGNIAGPLLFFFLLNAIRLSTKYYGLK
YGYVKGMEILQDLAGNRIQKLTEGASILGLFVMGALVSKWTTINIPIVVSRIKDESGKVDVQTVQNVLDSIMPGALPLGL
TLLVAWMLRKGVNPLLIICGIFVIGILGYWAGFLA
>Mature_275_residues
MEKEKRLTKKEIFSMFIRSNFLLGSFNFERVQAMGYCYVMIPAIKKLYGPGAKRNEALQRHLEWFNTHPWLTAPIFGVTA
AMEEEMANNKGIDGKAISGMKIGLMGPIAGVGDPIFWGTIRPVLAALGASLALGGNIAGPLLFFFLLNAIRLSTKYYGLK
YGYVKGMEILQDLAGNRIQKLTEGASILGLFVMGALVSKWTTINIPIVVSRIKDESGKVDVQTVQNVLDSIMPGALPLGL
TLLVAWMLRKGVNPLLIICGIFVIGILGYWAGFLA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain

Homologues:

Organism=Escherichia coli, GI1788122, Length=272, Percent_Identity=61.0294117647059, Blast_Score=350, Evalue=4e-98,
Organism=Escherichia coli, GI1789529, Length=277, Percent_Identity=36.4620938628159, Blast_Score=158, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTFD_BACSU (P26382)

Other databases:

- EMBL:   X56098
- EMBL:   X92868
- EMBL:   AL009126
- PIR:   S11401
- RefSeq:   NP_390582.1
- ProteinModelPortal:   P26382
- EnsemblBacteria:   EBBACT00000001538
- GeneID:   937595
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27040
- NMPDR:   fig|224308.1.peg.2707
- GenoList:   BSU27040
- GeneTree:   EBGT00050000006024
- HOGENOM:   HBG421686
- OMA:   VTMAMEE
- PhylomeDB:   P26382
- ProtClustDB:   CLSK806696
- BioCyc:   BSUB:BSU27040-MONOMER
- InterPro:   IPR004704
- InterPro:   IPR018405
- TIGRFAMs:   TIGR00828

Pfam domain/function: PF03613 EIID-AGA

EC number: NA

Molecular weight: Translated: 30087; Mature: 30087

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11286a44)-; HASH(0x1059ae44)-; HASH(0x10d8c558)-; HASH(0xfcc18b4)-; HASH(0x114387a4)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEKRLTKKEIFSMFIRSNFLLGSFNFERVQAMGYCYVMIPAIKKLYGPGAKRNEALQR
CCHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
HLEWFNTHPWLTAPIFGVTAAMEEEMANNKGIDGKAISGMKIGLMGPIAGVGDPIFWGTI
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHH
RPVLAALGASLALGGNIAGPLLFFFLLNAIRLSTKYYGLKYGYVKGMEILQDLAGNRIQK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LTEGASILGLFVMGALVSKWTTINIPIVVSRIKDESGKVDVQTVQNVLDSIMPGALPLGL
HHHHHHHHHHHHHHHHHHHCEEECHHEEEEEECCCCCCEEHHHHHHHHHHHCCCHHHHHH
TLLVAWMLRKGVNPLLIICGIFVIGILGYWAGFLA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MEKEKRLTKKEIFSMFIRSNFLLGSFNFERVQAMGYCYVMIPAIKKLYGPGAKRNEALQR
CCHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
HLEWFNTHPWLTAPIFGVTAAMEEEMANNKGIDGKAISGMKIGLMGPIAGVGDPIFWGTI
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHH
RPVLAALGASLALGGNIAGPLLFFFLLNAIRLSTKYYGLKYGYVKGMEILQDLAGNRIQK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LTEGASILGLFVMGALVSKWTTINIPIVVSRIKDESGKVDVQTVQNVLDSIMPGALPLGL
HHHHHHHHHHHHHHHHHHHCEEECHHEEEEEECCCCCCEEHHHHHHHHHHHCCCHHHHHH
TLLVAWMLRKGVNPLLIICGIFVIGILGYWAGFLA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377