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The map label for this gene is levF

Identifier: 16079759

GI number: 16079759

Start: 2761081

End: 2761890

Strand: Reverse

Name: levF

Synonym: BSU27050

Alternate gene names: 16079759

Gene position: 2761890-2761081 (Counterclockwise)

Preceding gene: 16079760

Following gene: 16079758

Centisome position: 65.52

GC content: 45.8

Gene sequence:

>810_bases
ATGTCTTCATTACAAATTATTTTGTTGTTAATCATTGCAGCAATCACCGGAATCGCAAGTGTATTGGATGAAGGGCAGAC
ACACCGGCCGCTAGTGGCCTGTACGTTGGTTGGCTTGGTGCTCGGGGACTTAAAAACGGGAATTATTCTTGGAGGCACAC
TGGAGCTGATGGCGCTCGGCTGGATGAACGTCGGGCTTGCCATGGCACCTGATACAGCGATCGCCTCTGTCATCTCAACC
ATTTTAGTTATTACCGCTGATCAAGGAATTGGCGAAGGGATTGCTGTCGCTGTTGCTTTAGCAGCTGCGGGTCAGGCGCT
GACGATTTTTGTTCGGACGATCACTGTCTTTTTCATCCACCGCGCTGATCAATATGCAAAAGCGGGAAATATAAAAGGAA
TTGAAATTATGCATATTACCGCGATGGTGTTTCAAGCTTTGCGCGTCATGATTCCTACATTGATTGTTGCTTTAATTAGT
GTCAGCGCTGTTCAGGCGTTCCTGGGAAATATTCCTGATGTCATTACAAAAGGACTGCAAATAGGCGGAGGCATTATTGT
GGTTGTCGGGTATGCCATGGTGATCAATATGATGAATATTCCTTATTTAAAGCCATTTTTCTATATTGGTTTCTTATTAG
CGGCGTTTACCGATTTTAACTTAGTGGGATTTGGAGCCCTTGGTCTCTGTCTGGCGCTTTTATACCAGCAGGTGATGCAA
AAACAAAGCGCTCACGGAGCAGTCGCGGCCGCATCAGACAGTGGCAGTGTTGCTGTTTATGATGACGATGATGATGATCT
TGATGCCTAA

Upstream 100 bases:

>100_bases
AAGGGCGTGAAACTGGAATTGAGGCAGCTGCCTTCAGATGCCAGTGAGGATTTTGTACAGATTTTACGGAACGTAACAAA
ATAATCAAGGGGATGAGAAT

Downstream 100 bases:

>100_bases
ACGATGAGGGGGAAGAAAAAATGGAGAAAGAAAAACGATTAACGAAGAAAGAAATTTTCAGCATGTTTATTCGTTCAAAT
TTTTTACTCGGTTCCTTTAA

Product: phosphotransferase system (PTS) fructose-specific enzyme IIC component

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Fru; PTS system fructose-specific EIIC component; p28

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSSLQIILLLIIAAITGIASVLDEGQTHRPLVACTLVGLVLGDLKTGIILGGTLELMALGWMNVGLAMAPDTAIASVIST
ILVITADQGIGEGIAVAVALAAAGQALTIFVRTITVFFIHRADQYAKAGNIKGIEIMHITAMVFQALRVMIPTLIVALIS
VSAVQAFLGNIPDVITKGLQIGGGIIVVVGYAMVINMMNIPYLKPFFYIGFLLAAFTDFNLVGFGALGLCLALLYQQVMQ
KQSAHGAVAAASDSGSVAVYDDDDDDLDA

Sequences:

>Translated_269_residues
MSSLQIILLLIIAAITGIASVLDEGQTHRPLVACTLVGLVLGDLKTGIILGGTLELMALGWMNVGLAMAPDTAIASVIST
ILVITADQGIGEGIAVAVALAAAGQALTIFVRTITVFFIHRADQYAKAGNIKGIEIMHITAMVFQALRVMIPTLIVALIS
VSAVQAFLGNIPDVITKGLQIGGGIIVVVGYAMVINMMNIPYLKPFFYIGFLLAAFTDFNLVGFGALGLCLALLYQQVMQ
KQSAHGAVAAASDSGSVAVYDDDDDDLDA
>Mature_268_residues
SSLQIILLLIIAAITGIASVLDEGQTHRPLVACTLVGLVLGDLKTGIILGGTLELMALGWMNVGLAMAPDTAIASVISTI
LVITADQGIGEGIAVAVALAAAGQALTIFVRTITVFFIHRADQYAKAGNIKGIEIMHITAMVFQALRVMIPTLIVALISV
SAVQAFLGNIPDVITKGLQIGGGIIVVVGYAMVINMMNIPYLKPFFYIGFLLAAFTDFNLVGFGALGLCLALLYQQVMQK
QSAHGAVAAASDSGSVAVYDDDDDDLDA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3715

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-4 domain

Homologues:

Organism=Escherichia coli, GI1788121, Length=270, Percent_Identity=57.7777777777778, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI1789528, Length=258, Percent_Identity=25.1937984496124, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTFC_BACSU (P26381)

Other databases:

- EMBL:   X56098
- EMBL:   X92868
- EMBL:   AL009126
- PIR:   S11400
- RefSeq:   NP_390583.1
- ProteinModelPortal:   P26381
- EnsemblBacteria:   EBBACT00000003046
- GeneID:   937602
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27050
- NMPDR:   fig|224308.1.peg.2708
- GenoList:   BSU27050
- GeneTree:   EBGT00050000006019
- HOGENOM:   HBG417063
- OMA:   MIVRTAT
- PhylomeDB:   P26381
- ProtClustDB:   PRK15065
- BioCyc:   BSUB:BSU27050-MONOMER
- InterPro:   IPR004700
- InterPro:   IPR018404
- TIGRFAMs:   TIGR00822

Pfam domain/function: PF03609 EII-Sor

EC number: NA

Molecular weight: Translated: 28020; Mature: 27888

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS51106 PTS_EIIC_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x110819f8)-; HASH(0xf096254)-; HASH(0x11323438)-; HASH(0x10ea111c)-; HASH(0x11383124)-; HASH(0x10dfcaf4)-; HASH(0x11455e64)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLQIILLLIIAAITGIASVLDEGQTHRPLVACTLVGLVLGDLKTGIILGGTLELMALG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHH
WMNVGLAMAPDTAIASVISTILVITADQGIGEGIAVAVALAAAGQALTIFVRTITVFFIH
HHHCCCEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RADQYAKAGNIKGIEIMHITAMVFQALRVMIPTLIVALISVSAVQAFLGNIPDVITKGLQ
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IGGGIIVVVGYAMVINMMNIPYLKPFFYIGFLLAAFTDFNLVGFGALGLCLALLYQQVMQ
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KQSAHGAVAAASDSGSVAVYDDDDDDLDA
HHCCCCCEEEECCCCCEEEECCCCCCCCH
>Mature Secondary Structure 
SSLQIILLLIIAAITGIASVLDEGQTHRPLVACTLVGLVLGDLKTGIILGGTLELMALG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHH
WMNVGLAMAPDTAIASVISTILVITADQGIGEGIAVAVALAAAGQALTIFVRTITVFFIH
HHHCCCEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RADQYAKAGNIKGIEIMHITAMVFQALRVMIPTLIVALISVSAVQAFLGNIPDVITKGLQ
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IGGGIIVVVGYAMVINMMNIPYLKPFFYIGFLLAAFTDFNLVGFGALGLCLALLYQQVMQ
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KQSAHGAVAAASDSGSVAVYDDDDDDLDA
HHCCCCCEEEECCCCCEEEECCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377