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The map label for this gene is sacC

Identifier: 16079757

GI number: 16079757

Start: 2758043

End: 2760076

Strand: Reverse

Name: sacC

Synonym: BSU27030

Alternate gene names: 16079757

Gene position: 2760076-2758043 (Counterclockwise)

Preceding gene: 16079758

Following gene: 255767622

Centisome position: 65.47

GC content: 45.48

Gene sequence:

>2034_bases
ATGAAAAAGAGACTGATTCAAGTCATGATCATGTTCACCCTGCTGTTGACTATGGCATTTTCGGCAGATGCAGCCGATTC
AAGCTACTATGATGAGGATTACCGTCCTCAATATCACTTCACACCGGAGGCAAACTGGATGAATGACCCAAACGGAATGG
TATATTATGCTGGGGAATATCACTTGTTCTATCAATACCATCCATACGGGCTTCAGTGGGGGCCCATGCATTGGGGGCAT
GCCGTCAGCAAAGATTTGGTTACATGGGAACACCTTCCTGTTGCGCTGTATCCGGATGAAAAAGGCACGATCTTTTCTGG
AAGCGCAGTTGTAGATAAAAATAACACAAGCGGTTTTCAAACAGGCAAAGAGAAGCCGCTTGTGGCCATTTATACACAGG
ATCGGGAAGGCCATCAAGTGCAAAGTATTGCCTATAGCAACGACAAAGGAAGAACATGGACGAAGTACGCTGGCAACCCT
GTCATTCCAAACCCGGGTAAAAAAGATTTTCGCGATCCAAAAGTCTTTTGGTATGAGAAAGAAAAAAAGTGGGTGATGGT
GCTTGCGGCCGGTGACCGAATCCTCATTTATACATCAAAAAATCTGAAGCAGTGGACGTATGCAAGTGAATTTGGACAGG
ATCAAGGAAGCCACGGGGGAGTATGGGAATGCCCGGATTTATTTGAGCTTCCGGTAGACGGCAATCCGAATCAAAAGAAA
TGGGTCATGCAGGTCAGTGTCGGAAACGGAGCGGTCTCGGGAGGATCAGGCATGCAATATTTTGTAGGGGACTTTGATGG
AACTCATTTTAAAAATGAAAACCCGCCAAACAAAGTTCTCTGGACGGATTACGGCAGAGACTTTTATGCGGCTGTATCCT
GGTCTGATATTCCATCCACAGACAGCCGCCGGCTATGGTTAGGGTGGATGAGCAATTGGCAATATGCGAATGATGTTCCG
ACATCCCCATGGAGAAGTGCAACGTCCATTCCAAGAGAGTTAAAATTGAAAGCGTTTACCGAAGGGGTTAGAGTGGTCCA
AACACCGGTGAAAGAGCTGGAAACCATTCGCGGAACCTCTAAGAAGTGGAAGAATCTGACCATATCGCCTGCAAGTCATA
ATGTGTTAGCGGGGCAATCTGGAGATGCCTATGAAATAAATGCAGAATTTAAAGTAAGCCCTGGTTCAGCTGCTGAATTT
GGTTTTAAGGTCCGAACAGGTGAAAATCAATTTACGAAGGTCGGCTATGACCGAAGGAACGCCAAATTGTTCGTTGACCG
GAGCGAGTCAGGCAACGACACCTTTAATCCGGCCTTTAACACCGGAAAAGAAACAGCCCCGTTGAAGCCGGTAAATGGGA
AGGTTAAGTTGCGCATTTTTGTTGACCGCTCCTCGGTTGAAGTATTTGGGAATGACGGAAAGCAGGTCATAACGGATATT
ATTCTCCCAGACCGATCAAGCAAAGGGCTTGAATTATATGCTGCAAATGGCGGTGTAAAGGTAAAATCTTTAACGATACA
CCCTTTAAAAAAGGTATGGGGAACGACACCTTTTATGTCCAATATGACTGGCTGGACGACTGTAAATGGCACGTGGGCAG
ACACAATTGAGGGGAAACAAGGGAGGTCGGACGGCGATTCCTTTATCTTGTCTTCAGCATCCGGGTCAGACTTCACTTAT
GAATCTGATATCACCATTAAGGATGGAAACGGAAGAGGGGCAGGAGCACTAATGTTTCGCTCTGACAAAGATGCCAAAAA
CGGTTACCTTGCCAATGTGGATGCGAAGCATGACCTAGTGAAATTCTTTAAATTTGAGAACGGTGCTGCTTCTGTCATTG
CTGAATACAAAACACCGATAGACGTTAATAAAAAGTATCATCTGAAAACAGAGGCCGAGGGCGATCGCTTTAAAATCTAT
TTAGATGATCGTCTTGTGATTGATGCACATGATTCCGTATTTTCAGAAGGCCAATTTGGCTTGAATGTGTGGGACGCGAC
TGCTGTCTTTCAGAATGTAACGAAGGAGTCTTAA

Upstream 100 bases:

>100_bases
GGATAACAGTGAAACAGAGGAGAATTGCCAGAAACGGACCAGCTTGCGCAGCAGTCTCTTTTGAAAGTGTTCAAACATGA
AGAGAGGAGCGAAGGAACAA

Downstream 100 bases:

>100_bases
TTTTAAAAAAGGCACAGACCCGAACAGTCTGTGCCTTCTTGTTATTTTAACAAGTTTAAAGATTCGCGGTTAAATGCTTC
CAAATCATCAGGTGTTCTGC

Product: levanase

Products: NA

Alternate protein names: Beta-D-fructofuranosidase; Exo-beta-D-fructosidase; Exo-levanase

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MKKRLIQVMIMFTLLLTMAFSADAADSSYYDEDYRPQYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLQWGPMHWGH
AVSKDLVTWEHLPVALYPDEKGTIFSGSAVVDKNNTSGFQTGKEKPLVAIYTQDREGHQVQSIAYSNDKGRTWTKYAGNP
VIPNPGKKDFRDPKVFWYEKEKKWVMVLAAGDRILIYTSKNLKQWTYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKK
WVMQVSVGNGAVSGGSGMQYFVGDFDGTHFKNENPPNKVLWTDYGRDFYAAVSWSDIPSTDSRRLWLGWMSNWQYANDVP
TSPWRSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTSKKWKNLTISPASHNVLAGQSGDAYEINAEFKVSPGSAAEF
GFKVRTGENQFTKVGYDRRNAKLFVDRSESGNDTFNPAFNTGKETAPLKPVNGKVKLRIFVDRSSVEVFGNDGKQVITDI
ILPDRSSKGLELYAANGGVKVKSLTIHPLKKVWGTTPFMSNMTGWTTVNGTWADTIEGKQGRSDGDSFILSSASGSDFTY
ESDITIKDGNGRGAGALMFRSDKDAKNGYLANVDAKHDLVKFFKFENGAASVIAEYKTPIDVNKKYHLKTEAEGDRFKIY
LDDRLVIDAHDSVFSEGQFGLNVWDATAVFQNVTKES

Sequences:

>Translated_677_residues
MKKRLIQVMIMFTLLLTMAFSADAADSSYYDEDYRPQYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLQWGPMHWGH
AVSKDLVTWEHLPVALYPDEKGTIFSGSAVVDKNNTSGFQTGKEKPLVAIYTQDREGHQVQSIAYSNDKGRTWTKYAGNP
VIPNPGKKDFRDPKVFWYEKEKKWVMVLAAGDRILIYTSKNLKQWTYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKK
WVMQVSVGNGAVSGGSGMQYFVGDFDGTHFKNENPPNKVLWTDYGRDFYAAVSWSDIPSTDSRRLWLGWMSNWQYANDVP
TSPWRSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTSKKWKNLTISPASHNVLAGQSGDAYEINAEFKVSPGSAAEF
GFKVRTGENQFTKVGYDRRNAKLFVDRSESGNDTFNPAFNTGKETAPLKPVNGKVKLRIFVDRSSVEVFGNDGKQVITDI
ILPDRSSKGLELYAANGGVKVKSLTIHPLKKVWGTTPFMSNMTGWTTVNGTWADTIEGKQGRSDGDSFILSSASGSDFTY
ESDITIKDGNGRGAGALMFRSDKDAKNGYLANVDAKHDLVKFFKFENGAASVIAEYKTPIDVNKKYHLKTEAEGDRFKIY
LDDRLVIDAHDSVFSEGQFGLNVWDATAVFQNVTKES
>Mature_677_residues
MKKRLIQVMIMFTLLLTMAFSADAADSSYYDEDYRPQYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLQWGPMHWGH
AVSKDLVTWEHLPVALYPDEKGTIFSGSAVVDKNNTSGFQTGKEKPLVAIYTQDREGHQVQSIAYSNDKGRTWTKYAGNP
VIPNPGKKDFRDPKVFWYEKEKKWVMVLAAGDRILIYTSKNLKQWTYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKK
WVMQVSVGNGAVSGGSGMQYFVGDFDGTHFKNENPPNKVLWTDYGRDFYAAVSWSDIPSTDSRRLWLGWMSNWQYANDVP
TSPWRSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTSKKWKNLTISPASHNVLAGQSGDAYEINAEFKVSPGSAAEF
GFKVRTGENQFTKVGYDRRNAKLFVDRSESGNDTFNPAFNTGKETAPLKPVNGKVKLRIFVDRSSVEVFGNDGKQVITDI
ILPDRSSKGLELYAANGGVKVKSLTIHPLKKVWGTTPFMSNMTGWTTVNGTWADTIEGKQGRSDGDSFILSSASGSDFTY
ESDITIKDGNGRGAGALMFRSDKDAKNGYLANVDAKHDLVKFFKFENGAASVIAEYKTPIDVNKKYHLKTEAEGDRFKIY
LDDRLVIDAHDSVFSEGQFGLNVWDATAVFQNVTKES

Specific function: Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Secreted

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=490, Percent_Identity=36.1224489795918, Blast_Score=274, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SACC_BACSU (P05656)

Other databases:

- EMBL:   X05649
- EMBL:   Y00485
- EMBL:   X92868
- EMBL:   AL009126
- EMBL:   X56098
- PIR:   A27286
- RefSeq:   NP_390581.1
- ProteinModelPortal:   P05656
- SMR:   P05656
- EnsemblBacteria:   EBBACT00000000922
- GeneID:   938092
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27030
- NMPDR:   fig|224308.1.peg.2706
- GenoList:   BSU27030
- GeneTree:   EBGT00050000001979
- HOGENOM:   HBG693902
- OMA:   SGAMYFV
- PhylomeDB:   P05656
- ProtClustDB:   CLSK376321
- BioCyc:   BSUB:BSU27030-MONOMER
- BRENDA:   3.2.1.65
- InterPro:   IPR008985
- InterPro:   IPR010496
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR011040
- Gene3D:   G3DSA:2.120.10.10
- SMART:   SM00640

Pfam domain/function: PF06439 DUF1080; PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N; SSF49899 ConA_like_lec_gl

EC number: =3.2.1.80

Molecular weight: Translated: 75952; Mature: 75952

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: ACT_SITE 49-49 BINDING 65-65 BINDING 73-73 BINDING 223-223 BINDING 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRLIQVMIMFTLLLTMAFSADAADSSYYDEDYRPQYHFTPEANWMNDPNGMVYYAGEY
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECEE
HLFYQYHPYGLQWGPMHWGHAVSKDLVTWEHLPVALYPDEKGTIFSGSAVVDKNNTSGFQ
EEEEEECCCCCEECCCCCCCHHHHCCEEEECCEEEEECCCCCCEEECCEEEECCCCCCCC
TGKEKPLVAIYTQDREGHQVQSIAYSNDKGRTWTKYAGNPVIPNPGKKDFRDPKVFWYEK
CCCCCCEEEEEECCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEC
EKKWVMVLAAGDRILIYTSKNLKQWTYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKK
CCCEEEEEEECCEEEEEECCCCCCEEEHHHHCCCCCCCCCEECCCCCEECCCCCCCCCCE
WVMQVSVGNGAVSGGSGMQYFVGDFDGTHFKNENPPNKVLWTDYGRDFYAAVSWSDIPST
EEEEEEECCCEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCC
DSRRLWLGWMSNWQYANDVPTSPWRSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTS
CCCEEEEEECCCCCCCCCCCCCCCCCHHCCCHHEEHHHHHCCHHEEHHHHHHHHHHCCCC
KKWKNLTISPASHNVLAGQSGDAYEINAEFKVSPGSAAEFGFKVRTGENQFTKVGYDRRN
CHHCEEEECCCCCCEEECCCCCEEEEEEEEEECCCCCHHCCEEEECCCCCEEECCCCCCC
AKLFVDRSESGNDTFNPAFNTGKETAPLKPVNGKVKLRIFVDRSSVEVFGNDGKQVITDI
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCCCCEEEEEE
ILPDRSSKGLELYAANGGVKVKSLTIHPLKKVWGTTPFMSNMTGWTTVNGTWADTIEGKQ
ECCCCCCCCEEEEECCCCEEEEEEEECHHHHHHCCCCCCCCCCCCEEECCEEEECCCCCC
GRSDGDSFILSSASGSDFTYESDITIKDGNGRGAGALMFRSDKDAKNGYLANVDAKHDLV
CCCCCCEEEEECCCCCCEEEECEEEEECCCCCCCEEEEEECCCCCCCCEEEECCCHHHHE
KFFKFENGAASVIAEYKTPIDVNKKYHLKTEAEGDRFKIYLDDRLVIDAHDSVFSEGQFG
EHEEECCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCEEEEECCCCHHCCCCCC
LNVWDATAVFQNVTKES
EEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MKKRLIQVMIMFTLLLTMAFSADAADSSYYDEDYRPQYHFTPEANWMNDPNGMVYYAGEY
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEECEE
HLFYQYHPYGLQWGPMHWGHAVSKDLVTWEHLPVALYPDEKGTIFSGSAVVDKNNTSGFQ
EEEEEECCCCCEECCCCCCCHHHHCCEEEECCEEEEECCCCCCEEECCEEEECCCCCCCC
TGKEKPLVAIYTQDREGHQVQSIAYSNDKGRTWTKYAGNPVIPNPGKKDFRDPKVFWYEK
CCCCCCEEEEEECCCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEC
EKKWVMVLAAGDRILIYTSKNLKQWTYASEFGQDQGSHGGVWECPDLFELPVDGNPNQKK
CCCEEEEEEECCEEEEEECCCCCCEEEHHHHCCCCCCCCCEECCCCCEECCCCCCCCCCE
WVMQVSVGNGAVSGGSGMQYFVGDFDGTHFKNENPPNKVLWTDYGRDFYAAVSWSDIPST
EEEEEEECCCEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCC
DSRRLWLGWMSNWQYANDVPTSPWRSATSIPRELKLKAFTEGVRVVQTPVKELETIRGTS
CCCEEEEEECCCCCCCCCCCCCCCCCHHCCCHHEEHHHHHCCHHEEHHHHHHHHHHCCCC
KKWKNLTISPASHNVLAGQSGDAYEINAEFKVSPGSAAEFGFKVRTGENQFTKVGYDRRN
CHHCEEEECCCCCCEEECCCCCEEEEEEEEEECCCCCHHCCEEEECCCCCEEECCCCCCC
AKLFVDRSESGNDTFNPAFNTGKETAPLKPVNGKVKLRIFVDRSSVEVFGNDGKQVITDI
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCCCCEEEEEE
ILPDRSSKGLELYAANGGVKVKSLTIHPLKKVWGTTPFMSNMTGWTTVNGTWADTIEGKQ
ECCCCCCCCEEEEECCCCEEEEEEEECHHHHHHCCCCCCCCCCCCEEECCEEEECCCCCC
GRSDGDSFILSSASGSDFTYESDITIKDGNGRGAGALMFRSDKDAKNGYLANVDAKHDLV
CCCCCCEEEEECCCCCCEEEECEEEEECCCCCCCEEEEEECCCCCCCCEEEECCCHHHHE
KFFKFENGAASVIAEYKTPIDVNKKYHLKTEAEGDRFKIYLDDRLVIDAHDSVFSEGQFG
EHEEECCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCEEEEECCCCHHCCCCCC
LNVWDATAVFQNVTKES
EEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3112519; 3120151; 9141695; 9384377; 2117666