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The map label for this gene is mleN

Identifier: 16079413

GI number: 16079413

Start: 2452800

End: 2454206

Strand: Reverse

Name: mleN

Synonym: BSU23560

Alternate gene names: 16079413

Gene position: 2454206-2452800 (Counterclockwise)

Preceding gene: 255767512

Following gene: 16079412

Centisome position: 58.22

GC content: 41.01

Gene sequence:

>1407_bases
TTGAAGGATGTAAGATTGCCAACACTATTTGAAATTATTATTGTACTAGGAGTATTTTTAGCACTTGTTCTATCGTTTAC
AGTCTTTCTCGACTTGCCGATACAGCTGGCGCTTTTTGTTTCATGGTTTATTGCAATGCTTTTAGGTATTAGACTCGGTT
ATTCTTATAAAGATTTACAAAATGCGATTGTACATGGGATATCAAATGGCCTTGAAGCCGTACTGATTCTCGTTTCAGTT
GGGGCGCTCATCGGAACATGGATTGCCGGAGGCGTTGTGCCGACTTTGATTTATTACGGACTGGAATTTATTCACCCAAG
TATCTTTTTACTGGCAACGTTAATTATTTGTTCCATTATGTCTGTGGCAACAGGAACGTCATGGGGAACAGTCGGAACTG
CCGGCATTGCCATGATCGCAATTGGAGAGGGGTTAGGCATTCCGCTTCCTCTAGTAGCGGGAGCTATTCTTTCAGGTGCT
TATTTTGGGGACAAGCTGTCTCCGCTGTCTGACAGTACAGTTCTAGCTTCTTCACTATCAAAAGTCGATGTATTGGCTCA
CGTGAGAGCAATGCTTTATTTATCAATACCTGCTTATGTCATTACAGCTATACTCTTTACAGTTGTTGGATTTATGTACG
GCGGGAAAAATATTGATCTTGATAAAGTTGAATTCTTAAAATCATCTTTGCAAAACACGTTTGACATTCATATTTGGATG
CTGATCCCGGCAGTTCTCGTCATAGTCTTATTGGCGATGAAAAAGCCATCTATGCCAGTTATCGTAATTGGCGCTTTACT
CGGTGCAATCTGGGCTGTTGTTTTCCAAGGAATGGATATTGCCCATGCGATTGCAACTGCGTACAACGGCTTCTCTATTA
AAACGGATGTTGAATTTTTGAACGGCTTGTTAAACCGCGGTGGAATTGTCGGTATGCTTGATTCTCTCGTCGTCATCATC
TTTGGCCTTGGTTTTGGCGGTCTGCTTGAGAAACTCGGCGTGTTAAAAGTCATTGTTTCAACGTTTGAAAAGAAATTAAC
TTCAGCCGGCAATGTCACATTGTCTACACTGATCGTAGCGTTTTTAGCAAACATCTTCGGCTGTGCGATGTATGTATCTC
TTATTTTGACTCCGAAAATTATGGAAGACAGCTATGATCGCTTACATCTGGACAGAAGAGTGTTATCACGTAACTCAGAG
GTCGGGGGAACATTGACTTCGGGGATGGTTCCTTGGTCTGATAATGGGATTTACATGGCTGGTATTTTAGGTGTTTCTAC
CTTCTCTTATCTGCCGTTTATGTGGCTCAGTTTTGTAGCAATAGGTCTTGCAATTATCTATGGTTATACAGGAAAATTTA
TATGGTATACGAAAAACAATACAGTTAAAGCCGAAAAACTAGGCTAA

Upstream 100 bases:

>100_bases
GATGAATAAGCTGTTTACAACATAGGGATGAGGAAGTATAGTATACGTGTTGCACTATATATTGAAAGCGTTTTAGCTTT
TACATGAAGGGGGACTTCTT

Downstream 100 bases:

>100_bases
TAGGGGAGGATCACTGAATGATAGCGAAACACATGATTCGGACACTGATGATCGAAACGCCTTCGGTTCCCGGAAATCTT
GGAAGGGTGGCTACGGCCAT

Product: malate-H+/Na+-lactate antiporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKDVRLPTLFEIIIVLGVFLALVLSFTVFLDLPIQLALFVSWFIAMLLGIRLGYSYKDLQNAIVHGISNGLEAVLILVSV
GALIGTWIAGGVVPTLIYYGLEFIHPSIFLLATLIICSIMSVATGTSWGTVGTAGIAMIAIGEGLGIPLPLVAGAILSGA
YFGDKLSPLSDSTVLASSLSKVDVLAHVRAMLYLSIPAYVITAILFTVVGFMYGGKNIDLDKVEFLKSSLQNTFDIHIWM
LIPAVLVIVLLAMKKPSMPVIVIGALLGAIWAVVFQGMDIAHAIATAYNGFSIKTDVEFLNGLLNRGGIVGMLDSLVVII
FGLGFGGLLEKLGVLKVIVSTFEKKLTSAGNVTLSTLIVAFLANIFGCAMYVSLILTPKIMEDSYDRLHLDRRVLSRNSE
VGGTLTSGMVPWSDNGIYMAGILGVSTFSYLPFMWLSFVAIGLAIIYGYTGKFIWYTKNNTVKAEKLG

Sequences:

>Translated_468_residues
MKDVRLPTLFEIIIVLGVFLALVLSFTVFLDLPIQLALFVSWFIAMLLGIRLGYSYKDLQNAIVHGISNGLEAVLILVSV
GALIGTWIAGGVVPTLIYYGLEFIHPSIFLLATLIICSIMSVATGTSWGTVGTAGIAMIAIGEGLGIPLPLVAGAILSGA
YFGDKLSPLSDSTVLASSLSKVDVLAHVRAMLYLSIPAYVITAILFTVVGFMYGGKNIDLDKVEFLKSSLQNTFDIHIWM
LIPAVLVIVLLAMKKPSMPVIVIGALLGAIWAVVFQGMDIAHAIATAYNGFSIKTDVEFLNGLLNRGGIVGMLDSLVVII
FGLGFGGLLEKLGVLKVIVSTFEKKLTSAGNVTLSTLIVAFLANIFGCAMYVSLILTPKIMEDSYDRLHLDRRVLSRNSE
VGGTLTSGMVPWSDNGIYMAGILGVSTFSYLPFMWLSFVAIGLAIIYGYTGKFIWYTKNNTVKAEKLG
>Mature_468_residues
MKDVRLPTLFEIIIVLGVFLALVLSFTVFLDLPIQLALFVSWFIAMLLGIRLGYSYKDLQNAIVHGISNGLEAVLILVSV
GALIGTWIAGGVVPTLIYYGLEFIHPSIFLLATLIICSIMSVATGTSWGTVGTAGIAMIAIGEGLGIPLPLVAGAILSGA
YFGDKLSPLSDSTVLASSLSKVDVLAHVRAMLYLSIPAYVITAILFTVVGFMYGGKNIDLDKVEFLKSSLQNTFDIHIWM
LIPAVLVIVLLAMKKPSMPVIVIGALLGAIWAVVFQGMDIAHAIATAYNGFSIKTDVEFLNGLLNRGGIVGMLDSLVVII
FGLGFGGLLEKLGVLKVIVSTFEKKLTSAGNVTLSTLIVAFLANIFGCAMYVSLILTPKIMEDSYDRLHLDRRVLSRNSE
VGGTLTSGMVPWSDNGIYMAGILGVSTFSYLPFMWLSFVAIGLAIIYGYTGKFIWYTKNNTVKAEKLG

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLEN_BACSU (P54571)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   B69967
- RefSeq:   NP_390237.1
- ProteinModelPortal:   P54571
- EnsemblBacteria:   EBBACT00000004018
- GeneID:   938720
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23560
- NMPDR:   fig|224308.1.peg.2361
- GenoList:   BSU23560
- GeneTree:   EBGT00050000000766
- HOGENOM:   HBG307651
- OMA:   IWATLFQ
- PhylomeDB:   P54571
- ProtClustDB:   CLSK874646
- BioCyc:   BSUB:BSU23560-MONOMER
- InterPro:   IPR004770
- InterPro:   IPR018461
- TIGRFAMs:   TIGR00931

Pfam domain/function: PF03553 Na_H_antiporter

EC number: NA

Molecular weight: Translated: 50213; Mature: 50213

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xf22d944)-; HASH(0x1002d4b4)-; HASH(0x10013278)-; HASH(0x107df7fc)-; HASH(0x10aafc0c)-; HASH(0x109bf518)-; HASH(0x10227288)-; HASH(0x10a309e4)-; HASH(0x1031496c)-; HASH(0x10204f24)-; HASH(0x101178b0)-; HASH(0x10179f8c)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCH
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309