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The map label for this gene is mleA

Identifier: 16079412

GI number: 16079412

Start: 2451463

End: 2452782

Strand: Reverse

Name: mleA

Synonym: BSU23550

Alternate gene names: 16079412

Gene position: 2452782-2451463 (Counterclockwise)

Preceding gene: 16079413

Following gene: 16079411

Centisome position: 58.18

GC content: 49.77

Gene sequence:

>1320_bases
ATGATAGCGAAACACATGATTCGGACACTGATGATCGAAACGCCTTCGGTTCCCGGAAATCTTGGAAGGGTGGCTACGGC
CATCGGCCTTCTGGGCGGGGATATTGGTGAAGTGGAGACGGTAAAAGTCGGACCCAATTACACAATGCGGAATATTACCG
TTCAAGTGGAGAATGAAGAACAGCTTCAGGAAGTGATCGCGGCTGTACAGGCCCTCGGAGAAGGGATCAGGCTTCACACC
GTATCTGATGAAGTCCTGTCCGCACATGAAGGCGGAAAAATCCAAATGAAAAGCAAAATGCCGATCCGTTCACTGGCAGA
GCTGGGACGTGTCTACACACCAGGTGTTGCGGACGTGTGCCGACTGATCGAAAAAGAACCTGAAAAAGCTTCTATTTATA
CAACGATCAGCAATTCTGTTGCAATTGTAACGGATGGAACCGCAATTTTAGGCCTTGGGAATATCGGCTCTGTGGCTGGC
ATGCCTGTCATGGAAGGAAAGGCTGCTTTATTTGATCAGCTGGCGGGTATTAGCGGCATTCCGATACTGCTTGATACGAG
TGATCCTGAAGAAATCATCAAAACAGTAAAACATATTTCTCCGGGCTTTAGCGGCATCCTTCTTGAGGATATCGGTTCGC
CGCATTGTTTTGAAATCGAGGATCGCCTAAAAGAGGAGCTGAACATCCCTGTTATGCACGATGACCAGCATGGTACAGCT
GTAGTGACGCTGGCAGCTGCCATTTCGGCATGCAGGAGTGCGGGGGTTGACCTGAAGGAAGCGAAAGTGGGGCAAATCGG
CCTTGGCGCAGCAGGCGTGGCCATCTGCCGGATGTTCATGGCTTATGGTGTGAATGCTGTATACGGAACAGACAAATCAG
AGTCAGCAATGAACCGTCTCGAACAATACGGCGGACAGGCAGTTTCCAGTATTGAGGAGCTTATGGAAACATGTGATATT
GTCATTGCTACAACGGGCGTTCCGGGTTTAATAAAACCGGCATTTGTACGTTCTGGTCAAGTGATTCTGGCTTTATCGAA
TCCGAAGCCGGAAATTGAGCCGGAGGCCGCACTGCAGGCTGGCGCCGCATATGCAGCTGACGGGCGTTCTGTTAATAATG
TACTCGGTTTTCCAGGGATTTTTAGAGGAGCGCTTAACGCAAAAAGCACGGAGATTAATCATGATATGCTTGTCGCAGCA
GCAGAGGCCATCGCAGCTTGCACTAAACAAGGTGATGTTGTCCCTCAGCCGCTTGATTCAAAAGTCCATCATGCGGTAGC
AGCAGCTGTCGAGCATGCAGCTCTTACAGCAGTGAAATAA

Upstream 100 bases:

>100_bases
GTCTTGCAATTATCTATGGTTATACAGGAAAATTTATATGGTATACGAAAAACAATACAGTTAAAGCCGAAAAACTAGGC
TAATAGGGGAGGATCACTGA

Downstream 100 bases:

>100_bases
ATGTAGCTAAACAGCACCTGTCGTAGCAGGTGCTGTTTTTTTCTTATCATTGTTCAATTTTGTTATCATTACTATGAAAA
TAAAAAAGGGAGTGATCACA

Product: NAD-dependent malic enzyme

Products: NA

Alternate protein names: NAD-ME 1

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MIAKHMIRTLMIETPSVPGNLGRVATAIGLLGGDIGEVETVKVGPNYTMRNITVQVENEEQLQEVIAAVQALGEGIRLHT
VSDEVLSAHEGGKIQMKSKMPIRSLAELGRVYTPGVADVCRLIEKEPEKASIYTTISNSVAIVTDGTAILGLGNIGSVAG
MPVMEGKAALFDQLAGISGIPILLDTSDPEEIIKTVKHISPGFSGILLEDIGSPHCFEIEDRLKEELNIPVMHDDQHGTA
VVTLAAAISACRSAGVDLKEAKVGQIGLGAAGVAICRMFMAYGVNAVYGTDKSESAMNRLEQYGGQAVSSIEELMETCDI
VIATTGVPGLIKPAFVRSGQVILALSNPKPEIEPEAALQAGAAYAADGRSVNNVLGFPGIFRGALNAKSTEINHDMLVAA
AEAIAACTKQGDVVPQPLDSKVHHAVAAAVEHAALTAVK

Sequences:

>Translated_439_residues
MIAKHMIRTLMIETPSVPGNLGRVATAIGLLGGDIGEVETVKVGPNYTMRNITVQVENEEQLQEVIAAVQALGEGIRLHT
VSDEVLSAHEGGKIQMKSKMPIRSLAELGRVYTPGVADVCRLIEKEPEKASIYTTISNSVAIVTDGTAILGLGNIGSVAG
MPVMEGKAALFDQLAGISGIPILLDTSDPEEIIKTVKHISPGFSGILLEDIGSPHCFEIEDRLKEELNIPVMHDDQHGTA
VVTLAAAISACRSAGVDLKEAKVGQIGLGAAGVAICRMFMAYGVNAVYGTDKSESAMNRLEQYGGQAVSSIEELMETCDI
VIATTGVPGLIKPAFVRSGQVILALSNPKPEIEPEAALQAGAAYAADGRSVNNVLGFPGIFRGALNAKSTEINHDMLVAA
AEAIAACTKQGDVVPQPLDSKVHHAVAAAVEHAALTAVK
>Mature_439_residues
MIAKHMIRTLMIETPSVPGNLGRVATAIGLLGGDIGEVETVKVGPNYTMRNITVQVENEEQLQEVIAAVQALGEGIRLHT
VSDEVLSAHEGGKIQMKSKMPIRSLAELGRVYTPGVADVCRLIEKEPEKASIYTTISNSVAIVTDGTAILGLGNIGSVAG
MPVMEGKAALFDQLAGISGIPILLDTSDPEEIIKTVKHISPGFSGILLEDIGSPHCFEIEDRLKEELNIPVMHDDQHGTA
VVTLAAAISACRSAGVDLKEAKVGQIGLGAAGVAICRMFMAYGVNAVYGTDKSESAMNRLEQYGGQAVSSIEELMETCDI
VIATTGVPGLIKPAFVRSGQVILALSNPKPEIEPEAALQAGAAYAADGRSVNNVLGFPGIFRGALNAKSTEINHDMLVAA
AEAIAACTKQGDVVPQPLDSKVHHAVAAAVEHAALTAVK

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family

Homologues:

Organism=Escherichia coli, GI1788806, Length=393, Percent_Identity=39.1857506361323, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI87081919, Length=428, Percent_Identity=25.7009345794392, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MAO1_BACSU (P54572)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- EMBL:   L06664
- PIR:   C69967
- RefSeq:   NP_390236.1
- ProteinModelPortal:   P54572
- SMR:   P54572
- EnsemblBacteria:   EBBACT00000001692
- GeneID:   938725
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23550
- NMPDR:   fig|224308.1.peg.2360
- GenoList:   BSU23550
- GeneTree:   EBGT00050000001535
- HOGENOM:   HBG285702
- OMA:   EDIGSPH
- ProtClustDB:   CLSK887521
- BioCyc:   BSUB:BSU23550-MONOMER
- BRENDA:   1.1.1.38
- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000106

Pfam domain/function: PF00390 malic; PF03949 Malic_M

EC number: =1.1.1.38

Molecular weight: Translated: 45890; Mature: 45890

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: ACT_SITE 112-112 ACT_SITE 167-167 BINDING 235-235 BINDING 347-347

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAKHMIRTLMIETPSVPGNLGRVATAIGLLGGDIGEVETVKVGPNYTMRNITVQVENEE
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEEECCHH
QLQEVIAAVQALGEGIRLHTVSDEVLSAHEGGKIQMKSKMPIRSLAELGRVYTPGVADVC
HHHHHHHHHHHHCCCEEEEEECHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHH
RLIEKEPEKASIYTTISNSVAIVTDGTAILGLGNIGSVAGMPVMEGKAALFDQLAGISGI
HHHHCCCCCCEEEEEECCCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCC
PILLDTSDPEEIIKTVKHISPGFSGILLEDIGSPHCFEIEDRLKEELNIPVMHDDQHGTA
EEEEECCCHHHHHHHHHHCCCCCCEEEHHHCCCCCEECHHHHHHHHCCCCEEECCCCCHH
VVTLAAAISACRSAGVDLKEAKVGQIGLGAAGVAICRMFMAYGVNAVYGTDKSESAMNRL
HHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHH
EQYGGQAVSSIEELMETCDIVIATTGVPGLIKPAFVRSGQVILALSNPKPEIEPEAALQA
HHHCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHCCCCCEEEEECCCCCCCCHHHHHHC
GAAYAADGRSVNNVLGFPGIFRGALNAKSTEINHDMLVAAAEAIAACTKQGDVVPQPLDS
CCEECCCCCCCHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCH
KVHHAVAAAVEHAALTAVK
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIAKHMIRTLMIETPSVPGNLGRVATAIGLLGGDIGEVETVKVGPNYTMRNITVQVENEE
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEEECCHH
QLQEVIAAVQALGEGIRLHTVSDEVLSAHEGGKIQMKSKMPIRSLAELGRVYTPGVADVC
HHHHHHHHHHHHCCCEEEEEECHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHH
RLIEKEPEKASIYTTISNSVAIVTDGTAILGLGNIGSVAGMPVMEGKAALFDQLAGISGI
HHHHCCCCCCEEEEEECCCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCC
PILLDTSDPEEIIKTVKHISPGFSGILLEDIGSPHCFEIEDRLKEELNIPVMHDDQHGTA
EEEEECCCHHHHHHHHHHCCCCCCEEEHHHCCCCCEECHHHHHHHHCCCCEEECCCCCHH
VVTLAAAISACRSAGVDLKEAKVGQIGLGAAGVAICRMFMAYGVNAVYGTDKSESAMNRL
HHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHH
EQYGGQAVSSIEELMETCDIVIATTGVPGLIKPAFVRSGQVILALSNPKPEIEPEAALQA
HHHCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHCCCCCEEEEECCCCCCCCHHHHHHC
GAAYAADGRSVNNVLGFPGIFRGALNAKSTEINHDMLVAAAEAIAACTKQGDVVPQPLDS
CCEECCCCCCCHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCH
KVHHAVAAAVEHAALTAVK
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8501064; 10903309