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The map label for this gene is aspA

Identifier: 255767512

GI number: 255767512

Start: 2454347

End: 2455774

Strand: Reverse

Name: aspA

Synonym: BSU23570

Alternate gene names: 255767512

Gene position: 2455774-2454347 (Counterclockwise)

Preceding gene: 16079415

Following gene: 16079413

Centisome position: 58.25

GC content: 45.87

Gene sequence:

>1428_bases
ATGTTAAACGGCCAAAAAGAATATCGCGTGGAAAAAGACTTCCTTGGGGAAAAACAAATTGAAGCAGATGTTTATTACGG
AATTCAGACGCTCCGTGCTTCTGAAAATTTTCCGATCACAGGATACAAAATCCATGAGGAAATGATTAACGCACTGGCGA
TTGTGAAAAAAGCTGCGGCTCTTGCCAACATGGACGTGAAACGGCTGTATGAAGGAATTGGCCAAGCTATCGTACAAGCC
GCTGACGAGATTCTGGAAGGCAAGTGGCACGATCAGTTTATCGTCGATCCGATTCAGGGCGGTGCCGGAACTTCTATGAA
CATGAACGCGAATGAGGTTATCGGAAACCGGGCGCTTGAAATCATGGGACATAAAAAGGGAGATTATATCCATTTAAGTC
CAAACACACATGTGAACATGTCACAGTCTACGAACGATGTGTTCCCGACTGCTATCCATATTTCCACATTGAAGCTCTTA
GAAAAACTGCTGAAAACAATGGAAGATATGCATAGTGTGTTTAAGCAAAAAGCACAGGAGTTTGACTCTGTTATTAAAAT
GGGCCGGACACACCTTCAAGATGCGGTTCCGATCCGTCTTGGCCAGGAATTCGAAGCTTACAGCCGTGTTCTCGAGCGTG
ATATCAAACGAATCAAGCAATCGCGCCAGCACCTGTATGAAGTCAACATGGGCGCAACTGCTGTTGGTACAGGGCTGAAC
GCTGATCCTGAATATATCAAACAGGTAGTAAAGCACCTTGCTGATATTAGCGGGCTTCCTCTTGTCGGCGCTGATCATCT
TGTTGATGCGACACAAAATACAGATGCCTATACAGAGGTATCAGCTTCATTAAAAGTCTGCATGATGAACATGTCGAAGA
TCGCAAACGACCTGCGCTTAATGGCGTCGGGACCGCGCGCCGGACTTGCGGAAATTTCTCTGCCTGCACGTCAGCCGGGT
TCATCTATTATGCCGGGGAAAGTCAATCCGGTTATGGCGGAGCTGATCAACCAAATTGCGTTCCAGGTTATCGGAAATGA
CAATACAATCTGCCTTGCTTCAGAAGCCGGCCAGCTTGAGTTGAACGTCATGGAGCCCGTGCTTGTCTTTAATTTGCTTC
AATCCATCAGCATCATGAACAACGGCTTCCGTTCGTTCACTGACAACTGCTTAAAAGGCATTGAAGCCAACGAAAAGCGT
ATGAAGCAATACGTAGAAAAAAGCGCAGGCGTGATCACAGCTGTCAATCCGCATCTTGGGTATGAAGCGGCAGCTAGAAT
TGCCAGGGAAGCAATTATGACAGGGCAATCTGTCCGGGATCTTTGTCTGCAGCATGATGTGCTGACTGAAGAAGAATTGG
ATATTATTTTAAACCCATATGAGATGACCAAACCAGGTATCGCAGGGAAAGAACTATTAGAAAAATAA

Upstream 100 bases:

>100_bases
TTCCTGTCGTCAAGAGAATTATGGAAACGCCGATAGCTGATGACGTTGTCCTGTAAAATGTAAAAACGATAACCAGCAAA
GAGAAAGAAGGTTAAAGATT

Downstream 100 bases:

>100_bases
AATTTGTATAAAATACAGCTTATTCATCTCCGAATAGTCGGATGAATAAGCTGTTTACAACATAGGGATGAGGAAGTATA
GTATACGTGTTGCACTATAT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MLNGQKEYRVEKDFLGEKQIEADVYYGIQTLRASENFPITGYKIHEEMINALAIVKKAAALANMDVKRLYEGIGQAIVQA
ADEILEGKWHDQFIVDPIQGGAGTSMNMNANEVIGNRALEIMGHKKGDYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLL
EKLLKTMEDMHSVFKQKAQEFDSVIKMGRTHLQDAVPIRLGQEFEAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLN
ADPEYIKQVVKHLADISGLPLVGADHLVDATQNTDAYTEVSASLKVCMMNMSKIANDLRLMASGPRAGLAEISLPARQPG
SSIMPGKVNPVMAELINQIAFQVIGNDNTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRSFTDNCLKGIEANEKR
MKQYVEKSAGVITAVNPHLGYEAAARIAREAIMTGQSVRDLCLQHDVLTEEELDIILNPYEMTKPGIAGKELLEK

Sequences:

>Translated_475_residues
MLNGQKEYRVEKDFLGEKQIEADVYYGIQTLRASENFPITGYKIHEEMINALAIVKKAAALANMDVKRLYEGIGQAIVQA
ADEILEGKWHDQFIVDPIQGGAGTSMNMNANEVIGNRALEIMGHKKGDYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLL
EKLLKTMEDMHSVFKQKAQEFDSVIKMGRTHLQDAVPIRLGQEFEAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLN
ADPEYIKQVVKHLADISGLPLVGADHLVDATQNTDAYTEVSASLKVCMMNMSKIANDLRLMASGPRAGLAEISLPARQPG
SSIMPGKVNPVMAELINQIAFQVIGNDNTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRSFTDNCLKGIEANEKR
MKQYVEKSAGVITAVNPHLGYEAAARIAREAIMTGQSVRDLCLQHDVLTEEELDIILNPYEMTKPGIAGKELLEK
>Mature_475_residues
MLNGQKEYRVEKDFLGEKQIEADVYYGIQTLRASENFPITGYKIHEEMINALAIVKKAAALANMDVKRLYEGIGQAIVQA
ADEILEGKWHDQFIVDPIQGGAGTSMNMNANEVIGNRALEIMGHKKGDYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLL
EKLLKTMEDMHSVFKQKAQEFDSVIKMGRTHLQDAVPIRLGQEFEAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLN
ADPEYIKQVVKHLADISGLPLVGADHLVDATQNTDAYTEVSASLKVCMMNMSKIANDLRLMASGPRAGLAEISLPARQPG
SSIMPGKVNPVMAELINQIAFQVIGNDNTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRSFTDNCLKGIEANEKR
MKQYVEKSAGVITAVNPHLGYEAAARIAREAIMTGQSVRDLCLQHDVLTEEELDIILNPYEMTKPGIAGKELLEK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily

Homologues:

Organism=Homo sapiens, GI19743875, Length=464, Percent_Identity=42.2413793103448, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI68303547, Length=221, Percent_Identity=29.8642533936652, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI31541964, Length=219, Percent_Identity=30.1369863013699, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI68303542, Length=219, Percent_Identity=30.1369863013699, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI87082375, Length=465, Percent_Identity=48.1720430107527, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=42.548596112311, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1790398, Length=209, Percent_Identity=28.2296650717703, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=43.0434782608696, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=41.8960244648318, Blast_Score=280, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=41.8655097613883, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640177, Length=464, Percent_Identity=42.2413793103448, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24640179, Length=464, Percent_Identity=42.2413793103448, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=41.9006479481641, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=40.948275862069, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=37.9310344827586, Blast_Score=332, Evalue=4e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASPA_BACSU (P26899)

Other databases:

- EMBL:   M63264
- EMBL:   D84432
- EMBL:   AL009126
- PIR:   B39440
- RefSeq:   NP_390238.2
- ProteinModelPortal:   P26899
- SMR:   P26899
- EnsemblBacteria:   EBBACT00000000486
- GeneID:   938721
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23570
- NMPDR:   fig|224308.1.peg.2362
- GenoList:   BSU23570
- GeneTree:   EBGT00070000031773
- HOGENOM:   HBG284369
- PhylomeDB:   P26899
- ProtClustDB:   PRK12273
- BioCyc:   BSUB:BSU23570-MONOMER
- BRENDA:   4.3.1.1
- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00839

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.1.1

Molecular weight: Translated: 52505; Mature: 52505

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: BINDING 104-104

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNGQKEYRVEKDFLGEKQIEADVYYGIQTLRASENFPITGYKIHEEMINALAIVKKAAA
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LANMDVKRLYEGIGQAIVQAADEILEGKWHDQFIVDPIQGGAGTSMNMNANEVIGNRALE
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
IMGHKKGDYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLLEKLLKTMEDMHSVFKQKAQE
HHCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSVIKMGRTHLQDAVPIRLGQEFEAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLN
HHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
ADPEYIKQVVKHLADISGLPLVGADHLVDATQNTDAYTEVSASLKVCMMNMSKIANDLRL
CCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MASGPRAGLAEISLPARQPGSSIMPGKVNPVMAELINQIAFQVIGNDNTICLASEAGQLE
HCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEE
LNVMEPVLVFNLLQSISIMNNGFRSFTDNCLKGIEANEKRMKQYVEKSAGVITAVNPHLG
EHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCC
YEAAARIAREAIMTGQSVRDLCLQHDVLTEEELDIILNPYEMTKPGIAGKELLEK
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCCCHHHHHCC
>Mature Secondary Structure
MLNGQKEYRVEKDFLGEKQIEADVYYGIQTLRASENFPITGYKIHEEMINALAIVKKAAA
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LANMDVKRLYEGIGQAIVQAADEILEGKWHDQFIVDPIQGGAGTSMNMNANEVIGNRALE
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
IMGHKKGDYIHLSPNTHVNMSQSTNDVFPTAIHISTLKLLEKLLKTMEDMHSVFKQKAQE
HHCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDSVIKMGRTHLQDAVPIRLGQEFEAYSRVLERDIKRIKQSRQHLYEVNMGATAVGTGLN
HHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
ADPEYIKQVVKHLADISGLPLVGADHLVDATQNTDAYTEVSASLKVCMMNMSKIANDLRL
CCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MASGPRAGLAEISLPARQPGSSIMPGKVNPVMAELINQIAFQVIGNDNTICLASEAGQLE
HCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEE
LNVMEPVLVFNLLQSISIMNNGFRSFTDNCLKGIEANEKRMKQYVEKSAGVITAVNPHLG
EHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCC
YEAAARIAREAIMTGQSVRDLCLQHDVLTEEELDIILNPYEMTKPGIAGKELLEK
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377