BacMap

Definition	Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession	NC_000964
Length	4,215,606

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The map label for this gene is ppiB

Identifier: 16079393

GI number: 16079393

Start: 2435360

End: 2435791

Strand: Reverse

Name: ppiB

Synonym: BSU23360

Alternate gene names: 16079393

Gene position: 2435791-2435360 (Counterclockwise)

Preceding gene: 16079395

Following gene: 16079391

Centisome position: 57.78

GC content: 46.3

Gene sequence:

>432_bases
TTGAAAACAGGTTACTTTTTATTAGAAGACGGGAACAAAATTGAATTTGAACTTTATCCTGAAGCTGCACCTGGAACTGT
TGCAAACTTTGAAAAGCTTGCGAACGAAGGCTTTTATGACGGGCTTACATTCCACCGAGTGATCCCTGGCTTCGTCAGCC
AAGGCGGATGCCCGCACGGAACTGGTACAGGCGGTCCGGGATACACAATCAAATGTGAAACAGAAGGCAACCCGCATACA
CATGAGGCGGGCGCACTTTCTATGGCACATGCCGGAAAAGACACTGGCGGCAGCCAATTTTTCATCGTTCATGAGCCACA
GCCTCATTTGAACGGTGTTCACACTGTATTTGGTAAAGTAACAAGCGGATTAGAATTCGCGAAAAACATGTCTAATGGCG
ATGTCATGAAAGAAGTTCGTGTAGAAGGCTAA

Upstream 100 bases:

>100_bases
CCCCCGAAAGGTGTGGTATAATAGCCGCGACAAGGAGAGACAGGCTATAACCTATACATAATCTCCTTTGAAAATTAAAA
TAAAGAAAGTAGGCATACAA

Downstream 100 bases:

>100_bases
TCGGCAGGCAAAAGGCTTCCTCTTAGAGAGGAAGCTTTTTTTATTGGCCAAAAATGTTCAATAAAAAACCGCCGAATGCT
GATGGCGGTTGGCAAAACCA

Product: peptidyl-prolyl isomerase

Products: NA

Alternate protein names: PPIase B; Rotamase B

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MKTGYFLLEDGNKIEFELYPEAAPGTVANFEKLANEGFYDGLTFHRVIPGFVSQGGCPHGTGTGGPGYTIKCETEGNPHT
HEAGALSMAHAGKDTGGSQFFIVHEPQPHLNGVHTVFGKVTSGLEFAKNMSNGDVMKEVRVEG

Sequences:

>Translated_143_residues
MKTGYFLLEDGNKIEFELYPEAAPGTVANFEKLANEGFYDGLTFHRVIPGFVSQGGCPHGTGTGGPGYTIKCETEGNPHT
HEAGALSMAHAGKDTGGSQFFIVHEPQPHLNGVHTVFGKVTSGLEFAKNMSNGDVMKEVRVEG
>Mature_143_residues
MKTGYFLLEDGNKIEFELYPEAAPGTVANFEKLANEGFYDGLTFHRVIPGFVSQGGCPHGTGTGGPGYTIKCETEGNPHT
HEAGALSMAHAGKDTGGSQFFIVHEPQPHLNGVHTVFGKVTSGLEFAKNMSNGDVMKEVRVEG

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain

Homologues:

Organism=Homo sapiens, GI7706339, Length=114, Percent_Identity=48.2456140350877, Blast_Score=99, Evalue=8e-22,
Organism=Homo sapiens, GI24308049, Length=125, Percent_Identity=44, Blast_Score=98, Evalue=3e-21,
Organism=Homo sapiens, GI64276486, Length=111, Percent_Identity=44.1441441441441, Blast_Score=92, Evalue=1e-19,
Organism=Homo sapiens, GI19557636, Length=122, Percent_Identity=44.2622950819672, Blast_Score=90, Evalue=6e-19,
Organism=Homo sapiens, GI10863927, Length=140, Percent_Identity=41.4285714285714, Blast_Score=85, Evalue=2e-17,
Organism=Homo sapiens, GI6631100, Length=134, Percent_Identity=41.7910447761194, Blast_Score=84, Evalue=4e-17,
Organism=Homo sapiens, GI4826932, Length=140, Percent_Identity=40, Blast_Score=84, Evalue=4e-17,
Organism=Homo sapiens, GI310127646, Length=140, Percent_Identity=39.2857142857143, Blast_Score=82, Evalue=1e-16,
Organism=Homo sapiens, GI310114371, Length=140, Percent_Identity=39.2857142857143, Blast_Score=82, Evalue=1e-16,
Organism=Homo sapiens, GI310119221, Length=140, Percent_Identity=39.2857142857143, Blast_Score=82, Evalue=1e-16,
Organism=Homo sapiens, GI4758950, Length=130, Percent_Identity=43.0769230769231, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI42560244, Length=139, Percent_Identity=38.8489208633094, Blast_Score=81, Evalue=3e-16,
Organism=Homo sapiens, GI256017253, Length=115, Percent_Identity=43.4782608695652, Blast_Score=77, Evalue=5e-15,
Organism=Homo sapiens, GI221139811, Length=115, Percent_Identity=43.4782608695652, Blast_Score=77, Evalue=5e-15,
Organism=Homo sapiens, GI256017251, Length=115, Percent_Identity=43.4782608695652, Blast_Score=77, Evalue=5e-15,
Organism=Homo sapiens, GI5174637, Length=149, Percent_Identity=37.5838926174497, Blast_Score=75, Evalue=1e-14,
Organism=Homo sapiens, GI178057341, Length=124, Percent_Identity=40.3225806451613, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI303305009, Length=147, Percent_Identity=38.0952380952381, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI45439316, Length=136, Percent_Identity=38.9705882352941, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI310129267, Length=137, Percent_Identity=37.956204379562, Blast_Score=74, Evalue=5e-14,
Organism=Homo sapiens, GI310120620, Length=137, Percent_Identity=37.956204379562, Blast_Score=74, Evalue=5e-14,
Organism=Homo sapiens, GI5031987, Length=124, Percent_Identity=41.1290322580645, Blast_Score=74, Evalue=5e-14,
Organism=Homo sapiens, GI4505991, Length=129, Percent_Identity=41.8604651162791, Blast_Score=72, Evalue=1e-13,
Organism=Homo sapiens, GI209417936, Length=124, Percent_Identity=39.5161290322581, Blast_Score=72, Evalue=1e-13,
Organism=Homo sapiens, GI30102944, Length=124, Percent_Identity=39.5161290322581, Blast_Score=72, Evalue=1e-13,
Organism=Homo sapiens, GI219842199, Length=124, Percent_Identity=39.5161290322581, Blast_Score=72, Evalue=1e-13,
Organism=Homo sapiens, GI169164185, Length=139, Percent_Identity=37.410071942446, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI169163623, Length=139, Percent_Identity=37.410071942446, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI169163387, Length=139, Percent_Identity=37.410071942446, Blast_Score=71, Evalue=3e-13,
Organism=Homo sapiens, GI150418007, Length=126, Percent_Identity=39.6825396825397, Blast_Score=71, Evalue=3e-13,
Organism=Homo sapiens, GI5454154, Length=131, Percent_Identity=37.4045801526718, Blast_Score=70, Evalue=6e-13,
Organism=Homo sapiens, GI310132444, Length=139, Percent_Identity=37.410071942446, Blast_Score=70, Evalue=6e-13,
Organism=Homo sapiens, GI310113152, Length=139, Percent_Identity=37.410071942446, Blast_Score=70, Evalue=6e-13,
Organism=Homo sapiens, GI310110104, Length=117, Percent_Identity=41.025641025641, Blast_Score=70, Evalue=6e-13,
Organism=Homo sapiens, GI20911035, Length=133, Percent_Identity=36.0902255639098, Blast_Score=70, Evalue=7e-13,
Organism=Homo sapiens, GI169214050, Length=139, Percent_Identity=35.9712230215827, Blast_Score=68, Evalue=2e-12,
Organism=Homo sapiens, GI310127745, Length=137, Percent_Identity=37.956204379562, Blast_Score=68, Evalue=3e-12,
Organism=Homo sapiens, GI310119290, Length=137, Percent_Identity=37.956204379562, Blast_Score=68, Evalue=3e-12,
Organism=Homo sapiens, GI239751853, Length=139, Percent_Identity=35.9712230215827, Blast_Score=68, Evalue=3e-12,
Organism=Homo sapiens, GI113428306, Length=139, Percent_Identity=35.9712230215827, Blast_Score=68, Evalue=3e-12,
Organism=Homo sapiens, GI310114540, Length=137, Percent_Identity=37.956204379562, Blast_Score=68, Evalue=3e-12,
Organism=Homo sapiens, GI22547212, Length=135, Percent_Identity=40, Blast_Score=67, Evalue=5e-12,
Organism=Homo sapiens, GI7657473, Length=135, Percent_Identity=40, Blast_Score=67, Evalue=5e-12,
Organism=Homo sapiens, GI22547215, Length=135, Percent_Identity=40, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI1789763, Length=134, Percent_Identity=38.8059701492537, Blast_Score=73, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17539496, Length=125, Percent_Identity=47.2, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71997782, Length=149, Percent_Identity=40.2684563758389, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17551282, Length=137, Percent_Identity=42.3357664233577, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17551284, Length=137, Percent_Identity=42.3357664233577, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17506311, Length=122, Percent_Identity=40.9836065573771, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17506307, Length=139, Percent_Identity=43.1654676258993, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17552780, Length=125, Percent_Identity=44.8, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17532641, Length=113, Percent_Identity=38.9380530973451, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17539498, Length=126, Percent_Identity=40.4761904761905, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71980590, Length=136, Percent_Identity=34.5588235294118, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71980594, Length=136, Percent_Identity=34.5588235294118, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17559074, Length=135, Percent_Identity=40.7407407407407, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565860, Length=135, Percent_Identity=38.5185185185185, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17552782, Length=137, Percent_Identity=36.4963503649635, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17566266, Length=131, Percent_Identity=38.9312977099237, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532643, Length=131, Percent_Identity=37.4045801526718, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323562, Length=126, Percent_Identity=46.031746031746, Blast_Score=92, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320359, Length=142, Percent_Identity=43.6619718309859, Blast_Score=85, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320510, Length=141, Percent_Identity=41.1347517730496, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323246, Length=141, Percent_Identity=40.4255319148936, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321848, Length=123, Percent_Identity=45.5284552845528, Blast_Score=78, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17986117, Length=121, Percent_Identity=45.4545454545455, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922376, Length=130, Percent_Identity=41.5384615384615, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24650807, Length=142, Percent_Identity=40.1408450704225, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357249, Length=111, Percent_Identity=41.4414414414414, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI20130249, Length=131, Percent_Identity=45.0381679389313, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45549139, Length=124, Percent_Identity=42.741935483871, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17647301, Length=127, Percent_Identity=40.9448818897638, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24664125, Length=141, Percent_Identity=39.7163120567376, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21357783, Length=141, Percent_Identity=39.7163120567376, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19922912, Length=122, Percent_Identity=45.0819672131148, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652460, Length=119, Percent_Identity=34.453781512605, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24586038, Length=133, Percent_Identity=36.8421052631579, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17136360, Length=126, Percent_Identity=38.0952380952381, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPIB_BACSU (P35137)

Other databases:

- EMBL:   L09228
- EMBL:   X73898
- EMBL:   AL009126
- PIR:   S45537
- RefSeq:   NP_390217.1
- ProteinModelPortal:   P35137
- SMR:   P35137
- EnsemblBacteria:   EBBACT00000000434
- GeneID:   938934
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23360
- NMPDR:   fig|224308.1.peg.2341
- GenoList:   BSU23360
- GeneTree:   EBGT00050000002333
- HOGENOM:   HBG610621
- OMA:   FGYKGCK
- ProtClustDB:   CLSK2752438
- BioCyc:   BSUB:BSU23360-MONOMER
- BRENDA:   5.2.1.8
- GO:   GO:0005737
- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130
- Gene3D:   G3DSA:2.40.100.10
- PRINTS:   PR00153

Pfam domain/function: PF00160 Pro_isomerase; SSF50891 CSA_PPIase

EC number: =5.2.1.8

Molecular weight: Translated: 15256; Mature: 15256

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00170 CSA_PPIASE_1; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTGYFLLEDGNKIEFELYPEAAPGTVANFEKLANEGFYDGLTFHRVIPGFVSQGGCPHG
CCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
TGTGGPGYTIKCETEGNPHTHEAGALSMAHAGKDTGGSQFFIVHEPQPHLNGVHTVFGKV
CCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
TSGLEFAKNMSNGDVMKEVRVEG
HHHHHHHHCCCCCCEEEEEECCC
>Mature Secondary Structure
MKTGYFLLEDGNKIEFELYPEAAPGTVANFEKLANEGFYDGLTFHRVIPGFVSQGGCPHG
CCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
TGTGGPGYTIKCETEGNPHTHEAGALSMAHAGKDTGGSQFFIVHEPQPHLNGVHTVFGKV
CCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
TSGLEFAKNMSNGDVMKEVRVEG
HHHHHHHHCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 8022278; 9384377; 8755892