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The map label for this gene is recQ

Identifier: 16079359

GI number: 16079359

Start: 2406922

End: 2408412

Strand: Reverse

Name: recQ

Synonym: BSU23020

Alternate gene names: 16079359

Gene position: 2408412-2406922 (Counterclockwise)

Preceding gene: 16079360

Following gene: 16079358

Centisome position: 57.13

GC content: 45.74

Gene sequence:

>1491_bases
ATGACTAAATTACAGCAAACGTTATATCAGTTTTTTGGTTTTACTTCCTTTAAAAAAGGGCAGCAGGACATTATTGAAAG
CATACTCAGCGGGAAGGATACCATTGCAATGCTCCCGACTGGGGGAGGGAAATCGCTTTGCTACCAGCTGCCTGGTTATA
TGCTTGATGGCATGGTATTAATCGTTTCTCCACTGCTTTCTTTAATGGAGGATCAGGTGCAGCAGCTCAAAGCCCGGGGG
GAAAAGCGTGCTGCAGCTTTGAACAGCATGCTTAACAGGCAGGAAAGACAATTTGTTTTAGAACATATTCATCGATATAA
ATTTTTGTACTTGTCTCCTGAAGCTTTACAGTCTCCATATGTATTGGAAAAATTGAAAAGCGTTCCGATTAGCCTATTTG
TTATTGACGAAGCGCATTGTATTTCTGAATGGGGACACGACTTCAGGCCTGATTATTCAAAGCTCGGACAGCTGAGAAAA
AAACTTGGACACCCACCCGTTCTGGCATTGACCGCCACGGCTACAAAGGAAACGCTGCAAGATGTCATGAACCTGCTGGA
GCTGCAGCATGCTGTACGCCATCTCAACTCAGTCAACCGCCCAAACATCGCACTGAGGGTTGAAAATGCGGCGGATACTG
CCGAAAAAATAGATAGGGTCATTCAGCTGGTTGAAAACCTGCAAGGTCCGGGAATTGTTTATTGTCCAACACGGAAATGG
GCTAAGGAATTAGCGGGTGAAATTAAAAGCAAAACGAGCAGCAGAGCCGACTTTTACCATGGCGGTTTGGAGTCAGGGGA
CAGGATTTTAATTCAGCAGCAGTTTATTCATAACCAGCTTGATGTGATATGTTGTACGAATGCGTTTGGAATGGGTGTCG
ATAAACCTGATATCAGATATGTGATTCATTTTCATCTCCCGCAGACGGCAGAAGCTTTTATGCAGGAAATCGGCAGAGCG
GGACGCGACGGAAAGCCGAGTGTCAGCATTTTGCTGCGGGCGCCAGGGGATTTTGAATTGCAGGAACAAATTATCCAGAT
GGAGAGTGTCACAGCTGAAGAAATCGCCGACGTAATCCGTGTGCTAGAGAAGACGGAGGAGCGGGATGAGAGAAGGCTGC
GTGACGTATTACTTCAGTATGGCGTCGGAGAGACACAGGCCCGTATGATGATTCATCTCTTTATGCAGGGAAAAACATCA
GTTGAACTGATGAAGAAAGAAATTTCATATCGCATGGAGCTGAAGCTGGAAAAAATGCACCGTGTATCATTTTTGCTTCA
GCGTGACGGCTGTTTAAGGCAGGCACTGTTAACCTACTTTGATGAATCATACGAGCCTGATGACGGAAACCTCCCGTGCT
GTTCACATTGCGGTTTTGATTTATCCTTGTATGAACAAAAAGGGGAACGGAGTAAAATGGCGCCATTAGACAGCTGGAGT
TCGGAATTGCACCGGATATTCAGCTTGCAGACTGTAGGTGAGCTGAACTGA

Upstream 100 bases:

>100_bases
CGGATGCGGACGAATAAAATCAAACAAATTCGCGACGGGCTGGAACAGCGGTTCAGCTACTTTCAAATCAGACTGGCATT
GACAAAGCAGGTGCAGCAAT

Downstream 100 bases:

>100_bases
AACAGCAAAAAGATTATATTCAGCATATGACTGACCATGATATGCTGAAGCAGTTATATTTGACGCAGCTACTCATCATC
TTTGCCGCTGCTGCAGCAGG

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: Recombination protein S

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTKLQQTLYQFFGFTSFKKGQQDIIESILSGKDTIAMLPTGGGKSLCYQLPGYMLDGMVLIVSPLLSLMEDQVQQLKARG
EKRAAALNSMLNRQERQFVLEHIHRYKFLYLSPEALQSPYVLEKLKSVPISLFVIDEAHCISEWGHDFRPDYSKLGQLRK
KLGHPPVLALTATATKETLQDVMNLLELQHAVRHLNSVNRPNIALRVENAADTAEKIDRVIQLVENLQGPGIVYCPTRKW
AKELAGEIKSKTSSRADFYHGGLESGDRILIQQQFIHNQLDVICCTNAFGMGVDKPDIRYVIHFHLPQTAEAFMQEIGRA
GRDGKPSVSILLRAPGDFELQEQIIQMESVTAEEIADVIRVLEKTEERDERRLRDVLLQYGVGETQARMMIHLFMQGKTS
VELMKKEISYRMELKLEKMHRVSFLLQRDGCLRQALLTYFDESYEPDDGNLPCCSHCGFDLSLYEQKGERSKMAPLDSWS
SELHRIFSLQTVGELN

Sequences:

>Translated_496_residues
MTKLQQTLYQFFGFTSFKKGQQDIIESILSGKDTIAMLPTGGGKSLCYQLPGYMLDGMVLIVSPLLSLMEDQVQQLKARG
EKRAAALNSMLNRQERQFVLEHIHRYKFLYLSPEALQSPYVLEKLKSVPISLFVIDEAHCISEWGHDFRPDYSKLGQLRK
KLGHPPVLALTATATKETLQDVMNLLELQHAVRHLNSVNRPNIALRVENAADTAEKIDRVIQLVENLQGPGIVYCPTRKW
AKELAGEIKSKTSSRADFYHGGLESGDRILIQQQFIHNQLDVICCTNAFGMGVDKPDIRYVIHFHLPQTAEAFMQEIGRA
GRDGKPSVSILLRAPGDFELQEQIIQMESVTAEEIADVIRVLEKTEERDERRLRDVLLQYGVGETQARMMIHLFMQGKTS
VELMKKEISYRMELKLEKMHRVSFLLQRDGCLRQALLTYFDESYEPDDGNLPCCSHCGFDLSLYEQKGERSKMAPLDSWS
SELHRIFSLQTVGELN
>Mature_495_residues
TKLQQTLYQFFGFTSFKKGQQDIIESILSGKDTIAMLPTGGGKSLCYQLPGYMLDGMVLIVSPLLSLMEDQVQQLKARGE
KRAAALNSMLNRQERQFVLEHIHRYKFLYLSPEALQSPYVLEKLKSVPISLFVIDEAHCISEWGHDFRPDYSKLGQLRKK
LGHPPVLALTATATKETLQDVMNLLELQHAVRHLNSVNRPNIALRVENAADTAEKIDRVIQLVENLQGPGIVYCPTRKWA
KELAGEIKSKTSSRADFYHGGLESGDRILIQQQFIHNQLDVICCTNAFGMGVDKPDIRYVIHFHLPQTAEAFMQEIGRAG
RDGKPSVSILLRAPGDFELQEQIIQMESVTAEEIADVIRVLEKTEERDERRLRDVLLQYGVGETQARMMIHLFMQGKTSV
ELMKKEISYRMELKLEKMHRVSFLLQRDGCLRQALLTYFDESYEPDDGNLPCCSHCGFDLSLYEQKGERSKMAPLDSWSS
ELHRIFSLQTVGELN

Specific function: Probable DNA helicase. Required in synaptic and/or post- synaptic stages of recombination. Probably has overlapping function with recQ (AC O34748). It probably acts to help generate ss-DNA from ds-DNA breaks

COG id: COG0514

COG function: function code L; Superfamily II DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

Organism=Homo sapiens, GI51242947, Length=372, Percent_Identity=38.1720430107527, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI51242943, Length=372, Percent_Identity=38.1720430107527, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI148612816, Length=385, Percent_Identity=36.6233766233766, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI110735439, Length=353, Percent_Identity=37.6770538243626, Blast_Score=235, Evalue=8e-62,
Organism=Homo sapiens, GI169218196, Length=353, Percent_Identity=37.6770538243626, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI14591902, Length=369, Percent_Identity=33.8753387533875, Blast_Score=229, Evalue=6e-60,
Organism=Homo sapiens, GI14591904, Length=369, Percent_Identity=33.8753387533875, Blast_Score=229, Evalue=6e-60,
Organism=Homo sapiens, GI4557365, Length=367, Percent_Identity=36.2397820163488, Blast_Score=219, Evalue=6e-57,
Organism=Homo sapiens, GI284005309, Length=361, Percent_Identity=37.1191135734072, Blast_Score=206, Evalue=5e-53,
Organism=Homo sapiens, GI4758138, Length=340, Percent_Identity=24.1176470588235, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI48994977, Length=337, Percent_Identity=42.7299703264095, Blast_Score=290, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI32564293, Length=341, Percent_Identity=39.8826979472141, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI25145561, Length=346, Percent_Identity=41.907514450867, Blast_Score=257, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI71987997, Length=369, Percent_Identity=37.940379403794, Blast_Score=251, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI71987993, Length=369, Percent_Identity=37.940379403794, Blast_Score=250, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI17552866, Length=333, Percent_Identity=36.6366366366366, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71995724, Length=370, Percent_Identity=25.6756756756757, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507121, Length=370, Percent_Identity=25.4054054054054, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323844, Length=353, Percent_Identity=35.6940509915014, Blast_Score=232, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6320224, Length=355, Percent_Identity=22.5352112676056, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324217, Length=322, Percent_Identity=24.5341614906832, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320400, Length=366, Percent_Identity=21.8579234972678, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646066, Length=345, Percent_Identity=41.1594202898551, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI24664226, Length=349, Percent_Identity=36.9627507163324, Blast_Score=223, Evalue=3e-58,
Organism=Drosophila melanogaster, GI62472181, Length=403, Percent_Identity=34.9875930521092, Blast_Score=222, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24664230, Length=403, Percent_Identity=34.9875930521092, Blast_Score=222, Evalue=5e-58,
Organism=Drosophila melanogaster, GI21358123, Length=350, Percent_Identity=36.8571428571429, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI21355075, Length=374, Percent_Identity=22.192513368984, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECS_BACSU (P50729)

Other databases:

- EMBL:   L47648
- EMBL:   AL009126
- PIR:   A69691
- RefSeq:   NP_390183.1
- ProteinModelPortal:   P50729
- SMR:   P50729
- EnsemblBacteria:   EBBACT00000000423
- GeneID:   938969
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23020
- NMPDR:   fig|224308.1.peg.2306
- GenoList:   BSU23020
- GeneTree:   EBGT00070000031730
- HOGENOM:   HBG651799
- OMA:   DEIMNRL
- PhylomeDB:   P50729
- ProtClustDB:   CLSK887507
- BioCyc:   BSUB:BSU23020-MONOMER
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR002464
- InterPro:   IPR004589
- InterPro:   IPR001650
- InterPro:   IPR014021
- PANTHER:   PTHR13710
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00614

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C

EC number: =3.6.4.12

Molecular weight: Translated: 56509; Mature: 56378

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00690 DEAH_ATP_HELICASE; PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLQQTLYQFFGFTSFKKGQQDIIESILSGKDTIAMLPTGGGKSLCYQLPGYMLDGMVL
CCHHHHHHHHHHCCCHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCHHHHHHHH
IVSPLLSLMEDQVQQLKARGEKRAAALNSMLNRQERQFVLEHIHRYKFLYLSPEALQSPY
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHCCCH
VLEKLKSVPISLFVIDEAHCISEWGHDFRPDYSKLGQLRKKLGHPPVLALTATATKETLQ
HHHHHHCCCEEEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHH
DVMNLLELQHAVRHLNSVNRPNIALRVENAADTAEKIDRVIQLVENLQGPGIVYCPTRKW
HHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHH
AKELAGEIKSKTSSRADFYHGGLESGDRILIQQQFIHNQLDVICCTNAFGMGVDKPDIRY
HHHHHHHHHHCCCCCCHHHCCCCCCCCEEEHHHHHHHCCCCEEEECCCCCCCCCCCCCEE
VIHFHLPQTAEAFMQEIGRAGRDGKPSVSILLRAPGDFELQEQIIQMESVTAEEIADVIR
EEEEECCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHH
VLEKTEERDERRLRDVLLQYGVGETQARMMIHLFMQGKTSVELMKKEISYRMELKLEKMH
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RVSFLLQRDGCLRQALLTYFDESYEPDDGNLPCCSHCGFDLSLYEQKGERSKMAPLDSWS
HHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHCCCCCCCCCCHHHH
SELHRIFSLQTVGELN
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKLQQTLYQFFGFTSFKKGQQDIIESILSGKDTIAMLPTGGGKSLCYQLPGYMLDGMVL
CHHHHHHHHHHCCCHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCHHHHHHHH
IVSPLLSLMEDQVQQLKARGEKRAAALNSMLNRQERQFVLEHIHRYKFLYLSPEALQSPY
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHCCCH
VLEKLKSVPISLFVIDEAHCISEWGHDFRPDYSKLGQLRKKLGHPPVLALTATATKETLQ
HHHHHHCCCEEEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHH
DVMNLLELQHAVRHLNSVNRPNIALRVENAADTAEKIDRVIQLVENLQGPGIVYCPTRKW
HHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHH
AKELAGEIKSKTSSRADFYHGGLESGDRILIQQQFIHNQLDVICCTNAFGMGVDKPDIRY
HHHHHHHHHHCCCCCCHHHCCCCCCCCEEEHHHHHHHCCCCEEEECCCCCCCCCCCCCEE
VIHFHLPQTAEAFMQEIGRAGRDGKPSVSILLRAPGDFELQEQIIQMESVTAEEIADVIR
EEEEECCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHH
VLEKTEERDERRLRDVLLQYGVGETQARMMIHLFMQGKTSVELMKKEISYRMELKLEKMH
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RVSFLLQRDGCLRQALLTYFDESYEPDDGNLPCCSHCGFDLSLYEQKGERSKMAPLDSWS
HHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHCCCCCCCCCCHHHH
SELHRIFSLQTVGELN
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 9642195