Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is hepT

Identifier: 16079331

GI number: 16079331

Start: 2381919

End: 2382965

Strand: Reverse

Name: hepT

Synonym: BSU22740

Alternate gene names: 16079331

Gene position: 2382965-2381919 (Counterclockwise)

Preceding gene: 16079332

Following gene: 16079330

Centisome position: 56.53

GC content: 41.93

Gene sequence:

>1047_bases
ATGTTAAATATCATTCGTTTACTGGCGGAGTCGCTGCCACGCATATCGGATGGAAATGAAAACACAGATGTTTGGGTGAA
TGATATGAAATTTAAAATGGCCTACTCTTTTTTAAATGACGATATTGATGTAATCGAAAGAGAACTTGAACAAACCGTAC
GTTCCGATTACCCGCTTTTAAGCGAGGCAGGTCTTCACCTGCTGCAGGCCGGAGGGAAACGTATTCGGCCTGTTTTCGTG
CTGCTTTCTGGCATGTTTGGCGATTATGATATTAATAAGATTAAATATGTCGCCGTCACTCTGGAAATGATTCACATGGC
ATCTTTGGTTCATGATGATGTCATTGATGATGCAGAGCTTCGCCGAGGAAAACCGACAATCAAAGCAAAGTGGGACAATC
GTATTGCGATGTACACAGGCGATTATATGCTTGCGGGATCTCTTGAAATGATGACGAGAATTAACGAACCGAAAGCCCAT
AGGATTTTGTCACAGACGATCGTTGAAGTTTGTCTAGGGGAAATTGAGCAGATCAAAGACAAATACAACATGGAACAAAA
TCTCAGAACGTATCTCCGCCGTATCAAAAGAAAAACAGCTCTCTTGATCGCGGTCAGCTGCCAGCTTGGTGCCATTGCGT
CTGGAGCTGATGAGAAGATTCATAAGGCATTGTACTGGTTTGGGTATTACGTCGGCATGTCTTATCAGATTATTGATGAT
ATTCTTGATTTTACTTCAACTGAGGAAGAGCTGGGTAAACCCGTAGGAGGAGATTTGCTTCAAGGAAACGTCACATTGCC
AGTGCTGTATGCCCTGAAAAATCCTGCATTAAAAAACCAGCTTAAATTGATTAACAGTGAGACAACGCAGGAACAGCTTG
AACCAATCATTGAAGAAATCAAAAAAACAGATGCAATTGAAGCATCTATGGCAGTAAGCGAAATGTATCTGCAGAAAGCT
TTTCAGAAATTAAACACGCTTCCTCGAGGGCGCGCACGCTCGTCTCTTGCAGCCATCGCAAAATATATCGGTAAAAGAAA
ATTTTAA

Upstream 100 bases:

>100_bases
GCGAAGAGCTATAAAGAATATTCTTGGCTTCAAGAATCAGCCAGAGATTTCCCTGGAATGAAGGAACTGGCAGGCCTGTT
TGAAGAGGCGGGCCTGAAAA

Downstream 100 bases:

>100_bases
TTTTTGTAGATATTAAGAAATTTCTCCGCCCCGGATTGATTAATTATCAGAAAGATGTTACGATTTCGGTGTGCAGTGTG
AAAGCCTATACATACATAAT

Product: heptaprenyl diphosphate synthase component II

Products: NA

Alternate protein names: HEPPP synthase subunit 2; Spore germination protein C3

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MLNIIRLLAESLPRISDGNENTDVWVNDMKFKMAYSFLNDDIDVIERELEQTVRSDYPLLSEAGLHLLQAGGKRIRPVFV
LLSGMFGDYDINKIKYVAVTLEMIHMASLVHDDVIDDAELRRGKPTIKAKWDNRIAMYTGDYMLAGSLEMMTRINEPKAH
RILSQTIVEVCLGEIEQIKDKYNMEQNLRTYLRRIKRKTALLIAVSCQLGAIASGADEKIHKALYWFGYYVGMSYQIIDD
ILDFTSTEEELGKPVGGDLLQGNVTLPVLYALKNPALKNQLKLINSETTQEQLEPIIEEIKKTDAIEASMAVSEMYLQKA
FQKLNTLPRGRARSSLAAIAKYIGKRKF

Sequences:

>Translated_348_residues
MLNIIRLLAESLPRISDGNENTDVWVNDMKFKMAYSFLNDDIDVIERELEQTVRSDYPLLSEAGLHLLQAGGKRIRPVFV
LLSGMFGDYDINKIKYVAVTLEMIHMASLVHDDVIDDAELRRGKPTIKAKWDNRIAMYTGDYMLAGSLEMMTRINEPKAH
RILSQTIVEVCLGEIEQIKDKYNMEQNLRTYLRRIKRKTALLIAVSCQLGAIASGADEKIHKALYWFGYYVGMSYQIIDD
ILDFTSTEEELGKPVGGDLLQGNVTLPVLYALKNPALKNQLKLINSETTQEQLEPIIEEIKKTDAIEASMAVSEMYLQKA
FQKLNTLPRGRARSSLAAIAKYIGKRKF
>Mature_348_residues
MLNIIRLLAESLPRISDGNENTDVWVNDMKFKMAYSFLNDDIDVIERELEQTVRSDYPLLSEAGLHLLQAGGKRIRPVFV
LLSGMFGDYDINKIKYVAVTLEMIHMASLVHDDVIDDAELRRGKPTIKAKWDNRIAMYTGDYMLAGSLEMMTRINEPKAH
RILSQTIVEVCLGEIEQIKDKYNMEQNLRTYLRRIKRKTALLIAVSCQLGAIASGADEKIHKALYWFGYYVGMSYQIIDD
ILDFTSTEEELGKPVGGDLLQGNVTLPVLYALKNPALKNQLKLINSETTQEQLEPIIEEIKKTDAIEASMAVSEMYLQKA
FQKLNTLPRGRARSSLAAIAKYIGKRKF

Specific function: Supplies heptaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinone menaquinone-7 (MQ-7)

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family

Homologues:

Organism=Homo sapiens, GI50659086, Length=323, Percent_Identity=30.3405572755418, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI4758430, Length=247, Percent_Identity=25.1012145748988, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI83700220, Length=247, Percent_Identity=25.1012145748988, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI169808399, Length=333, Percent_Identity=22.5225225225225, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789578, Length=302, Percent_Identity=32.4503311258278, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1786623, Length=254, Percent_Identity=31.1023622047244, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=316, Percent_Identity=31.9620253164557, Blast_Score=160, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6319475, Length=301, Percent_Identity=31.2292358803987, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6325188, Length=230, Percent_Identity=24.3478260869565, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24651612, Length=323, Percent_Identity=29.4117647058824, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI281365769, Length=210, Percent_Identity=24.2857142857143, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24660002, Length=210, Percent_Identity=24.2857142857143, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEPS2_BACSU (P31114)

Other databases:

- EMBL:   M80245
- EMBL:   AL009126
- PIR:   E69630
- RefSeq:   NP_390155.1
- ProteinModelPortal:   P31114
- SMR:   P31114
- EnsemblBacteria:   EBBACT00000001536
- GeneID:   939002
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22740
- NMPDR:   fig|224308.1.peg.2278
- GenoList:   BSU22740
- GeneTree:   EBGT00050000001433
- HOGENOM:   HBG732667
- OMA:   TRRGVDT
- PhylomeDB:   P31114
- ProtClustDB:   CLSK873092
- BioCyc:   BSUB:BSU22740-MONOMER
- BioCyc:   MetaCyc:MONOMER-13770
- BRENDA:   2.5.1.30
- InterPro:   IPR014119
- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949
- Gene3D:   G3DSA:1.10.600.10
- PANTHER:   PTHR12001
- TIGRFAMs:   TIGR02748

Pfam domain/function: PF00348 polyprenyl_synt; SSF48576 Terpenoid_synth

EC number: =2.5.1.30

Molecular weight: Translated: 39516; Mature: 39516

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00723 POLYPRENYL_SYNTHASE_1; PS00444 POLYPRENYL_SYNTHASE_2

Important sites: BINDING 73-73 BINDING 76-76 BINDING 105-105 BINDING 121-121 BINDING 122-122 BINDING 198-198 BINDING 199-199 BINDING 236-236

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MLNIIRLLAESLPRISDGNENTDVWVNDMKFKMAYSFLNDDIDVIERELEQTVRSDYPLL
CHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCH
SEAGLHLLQAGGKRIRPVFVLLSGMFGDYDINKIKYVAVTLEMIHMASLVHDDVIDDAEL
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
RRGKPTIKAKWDNRIAMYTGDYMLAGSLEMMTRINEPKAHRILSQTIVEVCLGEIEQIKD
HCCCCCEEEECCCEEEEEECCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
KYNMEQNLRTYLRRIKRKTALLIAVSCQLGAIASGADEKIHKALYWFGYYVGMSYQIIDD
HHCHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
ILDFTSTEEELGKPVGGDLLQGNVTLPVLYALKNPALKNQLKLINSETTQEQLEPIIEEI
HHHCCCCHHHHCCCCCCCCEECCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHH
KKTDAIEASMAVSEMYLQKAFQKLNTLPRGRARSSLAAIAKYIGKRKF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNIIRLLAESLPRISDGNENTDVWVNDMKFKMAYSFLNDDIDVIERELEQTVRSDYPLL
CHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCH
SEAGLHLLQAGGKRIRPVFVLLSGMFGDYDINKIKYVAVTLEMIHMASLVHDDVIDDAEL
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
RRGKPTIKAKWDNRIAMYTGDYMLAGSLEMMTRINEPKAHRILSQTIVEVCLGEIEQIKD
HCCCCCEEEECCCEEEEEECCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
KYNMEQNLRTYLRRIKRKTALLIAVSCQLGAIASGADEKIHKALYWFGYYVGMSYQIIDD
HHCHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
ILDFTSTEEELGKPVGGDLLQGNVTLPVLYALKNPALKNQLKLINSETTQEQLEPIIEEI
HHHCCCCHHHHCCCCCCCCEECCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHH
KKTDAIEASMAVSEMYLQKAFQKLNTLPRGRARSSLAAIAKYIGKRKF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 2121900; 9720033