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The map label for this gene is ndk

Identifier: 16079330

GI number: 16079330

Start: 2381354

End: 2381803

Strand: Reverse

Name: ndk

Synonym: BSU22730

Alternate gene names: 16079330

Gene position: 2381803-2381354 (Counterclockwise)

Preceding gene: 16079331

Following gene: 50812262

Centisome position: 56.5

GC content: 42.89

Gene sequence:

>450_bases
ATGATGGAAAAGACTTTTATCATGGTGAAACCAGACGGTGTCCAACGTCAGCTCATTGGGGACATTTTATCTAGATTCGA
ACGTAAGGGCTTACAATTAGCTGGCGCCAAGTTAATGAGAGTGACTGAACAAATGGCTGAGAAACACTACGCCGAACATC
AAGGTAAGCCTTTCTTCGGAGAGCTCGTTGAGTTTATTACTTCAGGACCTGTATTCGCAATGGTGTGGGAAGGCGAAAAT
GTCATTGAAGTGACGAGACAGCTGATCGGGAAAACAAACCCTAAAGAAGCTTTACCTGGTACGATTCGTGGGGATTATGG
CATGTTTGTCGGAAAAAACATCATCCACGGCTCTGATTCTCTCGAAAGTGCAGAACGCGAGATTAACATTTTCTTTAAGA
ATGAAGAATTAGTATCATATCAGCAGCTTATGGCAGGCTGGATCTATTAA

Upstream 100 bases:

>100_bases
AGAAATTTCTCCGCCCCGGATTGATTAATTATCAGAAAGATGTTACGATTTCGGTGTGCAGTGTGAAAGCCTATACATAC
ATAATGATGGGGTGGAGAAT

Downstream 100 bases:

>100_bases
TCATTGTGAAAATAAGCAACAGAGCCGGCAACATATAACGCCGCCGGTTTTTGTTCTGTTTTGTAAAGGTTTACATTAAA
GTGAATAAAGGGTTGAATGG

Product: nucleoside diphosphate kinase

Products: NA

Alternate protein names: NDK; NDP kinase; Nucleoside-2-P kinase

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MMEKTFIMVKPDGVQRQLIGDILSRFERKGLQLAGAKLMRVTEQMAEKHYAEHQGKPFFGELVEFITSGPVFAMVWEGEN
VIEVTRQLIGKTNPKEALPGTIRGDYGMFVGKNIIHGSDSLESAEREINIFFKNEELVSYQQLMAGWIY

Sequences:

>Translated_149_residues
MMEKTFIMVKPDGVQRQLIGDILSRFERKGLQLAGAKLMRVTEQMAEKHYAEHQGKPFFGELVEFITSGPVFAMVWEGEN
VIEVTRQLIGKTNPKEALPGTIRGDYGMFVGKNIIHGSDSLESAEREINIFFKNEELVSYQQLMAGWIY
>Mature_149_residues
MMEKTFIMVKPDGVQRQLIGDILSRFERKGLQLAGAKLMRVTEQMAEKHYAEHQGKPFFGELVEFITSGPVFAMVWEGEN
VIEVTRQLIGKTNPKEALPGTIRGDYGMFVGKNIIHGSDSLESAEREINIFFKNEELVSYQQLMAGWIY

Specific function: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate

COG id: COG0105

COG function: function code F; Nucleoside diphosphate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NDK family

Homologues:

Organism=Homo sapiens, GI37693993, Length=147, Percent_Identity=53.7414965986395, Blast_Score=184, Evalue=2e-47,
Organism=Homo sapiens, GI38045913, Length=148, Percent_Identity=54.0540540540541, Blast_Score=181, Evalue=2e-46,
Organism=Homo sapiens, GI4557797, Length=148, Percent_Identity=54.0540540540541, Blast_Score=181, Evalue=3e-46,
Organism=Homo sapiens, GI66392227, Length=148, Percent_Identity=52.7027027027027, Blast_Score=179, Evalue=5e-46,
Organism=Homo sapiens, GI66392205, Length=148, Percent_Identity=52.7027027027027, Blast_Score=179, Evalue=5e-46,
Organism=Homo sapiens, GI4505409, Length=148, Percent_Identity=52.7027027027027, Blast_Score=179, Evalue=5e-46,
Organism=Homo sapiens, GI66392192, Length=148, Percent_Identity=52.7027027027027, Blast_Score=179, Evalue=5e-46,
Organism=Homo sapiens, GI66392203, Length=148, Percent_Identity=52.7027027027027, Blast_Score=179, Evalue=6e-46,
Organism=Homo sapiens, GI4826862, Length=138, Percent_Identity=50, Blast_Score=166, Evalue=5e-42,
Organism=Homo sapiens, GI7019465, Length=133, Percent_Identity=33.8345864661654, Blast_Score=85, Evalue=2e-17,
Organism=Homo sapiens, GI37574614, Length=133, Percent_Identity=33.8345864661654, Blast_Score=85, Evalue=2e-17,
Organism=Homo sapiens, GI5031951, Length=138, Percent_Identity=34.0579710144928, Blast_Score=82, Evalue=1e-16,
Organism=Homo sapiens, GI4505413, Length=135, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=4e-15,
Organism=Homo sapiens, GI148839372, Length=138, Percent_Identity=31.1594202898551, Blast_Score=73, Evalue=9e-14,
Organism=Escherichia coli, GI1788866, Length=137, Percent_Identity=48.9051094890511, Blast_Score=145, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17506807, Length=148, Percent_Identity=47.2972972972973, Blast_Score=156, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71995094, Length=132, Percent_Identity=31.0606060606061, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322783, Length=147, Percent_Identity=56.4625850340136, Blast_Score=189, Evalue=2e-49,
Organism=Drosophila melanogaster, GI45549037, Length=147, Percent_Identity=54.421768707483, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI18860097, Length=134, Percent_Identity=34.3283582089552, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDK_BACSU (P31103)

Other databases:

- EMBL:   M80245
- EMBL:   AL009126
- PIR:   D69666
- RefSeq:   NP_390154.1
- ProteinModelPortal:   P31103
- SMR:   P31103
- PhosSite:   P31103
- EnsemblBacteria:   EBBACT00000001285
- GeneID:   938997
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22730
- NMPDR:   fig|224308.1.peg.2277
- GenoList:   BSU22730
- GeneTree:   EBGT00050000001953
- HOGENOM:   HBG445152
- OMA:   ERPFFGA
- ProtClustDB:   PRK00668
- BioCyc:   BSUB:BSU22730-MONOMER
- BRENDA:   2.7.4.6
- GO:   GO:0005737
- HAMAP:   MF_00451
- InterPro:   IPR001564
- InterPro:   IPR023005
- Gene3D:   G3DSA:3.30.70.141
- PANTHER:   PTHR11349
- PRINTS:   PR01243
- SMART:   SM00562

Pfam domain/function: PF00334 NDK; SSF54919 NDK

EC number: =2.7.4.6

Molecular weight: Translated: 16959; Mature: 16959

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00469 NDP_KINASES

Important sites: ACT_SITE 116-116 BINDING 10-10 BINDING 58-58 BINDING 86-86 BINDING 92-92 BINDING 103-103 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEKTFIMVKPDGVQRQLIGDILSRFERKGLQLAGAKLMRVTEQMAEKHYAEHQGKPFFG
CCCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ELVEFITSGPVFAMVWEGENVIEVTRQLIGKTNPKEALPGTIRGDYGMFVGKNIIHGSDS
HHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHCCCCCCCCCH
LESAEREINIFFKNEELVSYQQLMAGWIY
HHHHHHEEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MMEKTFIMVKPDGVQRQLIGDILSRFERKGLQLAGAKLMRVTEQMAEKHYAEHQGKPFFG
CCCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ELVEFITSGPVFAMVWEGENVIEVTRQLIGKTNPKEALPGTIRGDYGMFVGKNIIHGSDS
HHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHCCCCCCCCCH
LESAEREINIFFKNEELVSYQQLMAGWIY
HHHHHHEEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377