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The map label for this gene is yodR

Identifier: 16079030

GI number: 16079030

Start: 2143022

End: 2143675

Strand: Reverse

Name: yodR

Synonym: BSU19720

Alternate gene names: 16079030

Gene position: 2143675-2143022 (Counterclockwise)

Preceding gene: 16079031

Following gene: 16079029

Centisome position: 50.85

GC content: 45.57

Gene sequence:

>654_bases
ATGGGCTTGGGAGTAGCAGAAAGAGAACAGATTGCAAAACGCGCTGCTACTGAAATTAAGCAGGGCATGATTGTGAATCT
CGGTATCGGTATCCCTTCCTTGGTACCGAACTTTTTGAAGCCTGACATGCAGGTCATGTTTCAAGCGGAAAACGGTGTCC
TTGGCATTGGAGAAAGTCCCGAAAAGGGAGAAGAGGATGCGCATTTATGCAACGCCGCGGGATATCCTGTCCGCGCTGTA
AAAGGGGCTTCTTATTTTGATACAACCATGTCTTTTGCGATGATCAGAAAAGGCAAAATTGACATTACGATTTTAGGCGC
CCTGCAGGTGAGCCAATCAGGAGATTTGGCAAATTGGCTTGTTCCGGGAAAAAAGGTGCCTGGTATGGGCGGGGCGATGG
AGCTTGCCCAAAAAGCGAAAAAAGTGGTTGTCGTCATGAGTCATACAGATCAAAAGGGAAGGCCTAAATTAACAGAAAGA
TGTACGCTGCCATTAACTGCTGCAGGCTGTGTAGATTTGATTATTACCGAAAAAGCGGTTCTTGAGGTCGATAGCCATCA
CTTCATTTTAAAAGAGCTGATGAATGGCTCGACAATCGATGAGGTGACGAGGCTGACAGAAGCTGAAATCAAAATAGATA
TGCCTTTTTCTTAA

Upstream 100 bases:

>100_bases
CTGAGCGTATCGTTCCGATGGGGGAGATTTCTGAAGAAATGATTGTCACACCGGGGGTTTTTGTTGAGGGGGTTGTACGA
AGCGAGGGAGTGAAGTGGAA

Downstream 100 bases:

>100_bases
GAGGGGGAATGAGGATGCAAGACATTGAAAGAAAAGTAATCGAGTGGCTTGATCAGCATTCGGCCAAAGCCATTCGTCTT
CTCAAAAGGCTGATTGGGGA

Product: acyloate-acetoacetate CoA-transferase

Products: Acetate; Butanoyl-CoA [C]

Alternate protein names: Probable CoA-transferase subunit beta

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MGLGVAEREQIAKRAATEIKQGMIVNLGIGIPSLVPNFLKPDMQVMFQAENGVLGIGESPEKGEEDAHLCNAAGYPVRAV
KGASYFDTTMSFAMIRKGKIDITILGALQVSQSGDLANWLVPGKKVPGMGGAMELAQKAKKVVVVMSHTDQKGRPKLTER
CTLPLTAAGCVDLIITEKAVLEVDSHHFILKELMNGSTIDEVTRLTEAEIKIDMPFS

Sequences:

>Translated_217_residues
MGLGVAEREQIAKRAATEIKQGMIVNLGIGIPSLVPNFLKPDMQVMFQAENGVLGIGESPEKGEEDAHLCNAAGYPVRAV
KGASYFDTTMSFAMIRKGKIDITILGALQVSQSGDLANWLVPGKKVPGMGGAMELAQKAKKVVVVMSHTDQKGRPKLTER
CTLPLTAAGCVDLIITEKAVLEVDSHHFILKELMNGSTIDEVTRLTEAEIKIDMPFS
>Mature_216_residues
GLGVAEREQIAKRAATEIKQGMIVNLGIGIPSLVPNFLKPDMQVMFQAENGVLGIGESPEKGEEDAHLCNAAGYPVRAVK
GASYFDTTMSFAMIRKGKIDITILGALQVSQSGDLANWLVPGKKVPGMGGAMELAQKAKKVVVVMSHTDQKGRPKLTERC
TLPLTAAGCVDLIITEKAVLEVDSHHFILKELMNGSTIDEVTRLTEAEIKIDMPFS

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family

Homologues:

Organism=Homo sapiens, GI4557817, Length=207, Percent_Identity=52.6570048309179, Blast_Score=211, Evalue=4e-55,
Organism=Homo sapiens, GI11545841, Length=201, Percent_Identity=50.7462686567164, Blast_Score=202, Evalue=3e-52,
Organism=Escherichia coli, GI1788552, Length=205, Percent_Identity=43.4146341463415, Blast_Score=181, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17531517, Length=208, Percent_Identity=49.5192307692308, Blast_Score=202, Evalue=1e-52,
Organism=Drosophila melanogaster, GI21358325, Length=207, Percent_Identity=49.7584541062802, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24655794, Length=207, Percent_Identity=49.7584541062802, Blast_Score=201, Evalue=2e-52,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): YODR_BACSU (O34466)

Other databases:

- EMBL:   AF015775
- EMBL:   AF006665
- EMBL:   AL009126
- PIR:   D69904
- RefSeq:   NP_389853.1
- HSSP:   Q29551
- ProteinModelPortal:   O34466
- SMR:   O34466
- EnsemblBacteria:   EBBACT00000001614
- GeneID:   940048
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU19720
- NMPDR:   fig|224308.1.peg.1976
- GenoList:   BSU19720
- GeneTree:   EBGT00050000001287
- HOGENOM:   HBG353770
- OMA:   MGGAMEL
- PhylomeDB:   O34466
- ProtClustDB:   CLSK2460926
- BioCyc:   BSUB:BSU19720-MONOMER
- InterPro:   IPR012791
- InterPro:   IPR004165
- PANTHER:   PTHR13707
- SMART:   SM00882
- TIGRFAMs:   TIGR02428

Pfam domain/function: PF01144 CoA_trans

EC number: 2.8.3.8 [C]

Molecular weight: Translated: 23334; Mature: 23202

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01274 COA_TRANSF_2

Important sites: ACT_SITE 50-50

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLGVAEREQIAKRAATEIKQGMIVNLGIGIPSLVPNFLKPDMQVMFQAENGVLGIGESP
CCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCCEEEEEEECCCEEECCCCC
EKGEEDAHLCNAAGYPVRAVKGASYFDTTMSFAMIRKGKIDITILGALQVSQSGDLANWL
CCCCCCHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEC
VPGKKVPGMGGAMELAQKAKKVVVVMSHTDQKGRPKLTERCTLPLTAAGCVDLIITEKAV
CCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCHHHCCCCEEHHHHHHHEEECHHH
LEVDSHHFILKELMNGSTIDEVTRLTEAEIKIDMPFS
EEECCCHHHHHHHHCCCCHHHHHHHHHCEEEEECCCC
>Mature Secondary Structure 
GLGVAEREQIAKRAATEIKQGMIVNLGIGIPSLVPNFLKPDMQVMFQAENGVLGIGESP
CCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCCEEEEEEECCCEEECCCCC
EKGEEDAHLCNAAGYPVRAVKGASYFDTTMSFAMIRKGKIDITILGALQVSQSGDLANWL
CCCCCCHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEC
VPGKKVPGMGGAMELAQKAKKVVVVMSHTDQKGRPKLTERCTLPLTAAGCVDLIITEKAV
CCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCHHHCCCCEEHHHHHHHEEECHHH
LEVDSHHFILKELMNGSTIDEVTRLTEAEIKIDMPFS
EEECCCHHHHHHHHCCCCHHHHHHHHHCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acetyl-CoA; Butyrate [C]

Specific reaction: Acetyl-CoA + Butyrate (n-C4:0) --> Acetate + Butanoyl-CoA [C]

General reaction: Coenzyme A transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9734814; 9384377