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The map label for this gene is rseP

Identifier: 16078719

GI number: 16078719

Start: 1724029

End: 1725297

Strand: Direct

Name: rseP

Synonym: BSU16560

Alternate gene names: 16078719

Gene position: 1724029-1725297 (Clockwise)

Preceding gene: 255767375

Following gene: 16078720

Centisome position: 40.9

GC content: 43.74

Gene sequence:

>1269_bases
ATGTTCGTGAATACAGTTATAGCGTTTATCATTATTTTCGGAACGCTCGTTTTCTTCCATGAACTGGGCCATTTATTGCT
AGCCCAAAGAGCGGGAATTCTCTGCCGTGAATTTGCGATCGGCTTCGGTCCAAAGATTTTTTCTTTCAAAAAAAATGAAA
CAGTTTATACGATCAGGCTGCTTCCGGTCGGCGGATTTGTTCGTATGGCCGGCGAAGATCCGGAAATGATTGAGGTGAAA
CCCGGATACACGGTCGGGCTTCTGTTTAATAAGGAAGATCAAGTTGAGAAAGTCATCATCAATCAAAAGGAAAAATATCC
GGATGCTTTAGTCATTGAAGTGGAAACAGCGGATCTAGAGCATGACATGAAGATCACCGGTTATGAACAGGGGAAAGAGG
ACGAACTTTCCAGCTTTACTGTCAGCGAAACATCCTTTTTTATTGTAGACGGAGAAGAAGTGCAGATTGCGCCGTATAAT
CGCCAATTTGGTTCCAAACCTGTATGGCAGCGGATTAAAGCAATTGCTGCAGGGCCGATTATGAACTTTATTTTAGCTTA
CGTCATTTTAGTGATGCTTGGGCTGATTCAAGGCGTACCGTCAAATGAACCTATGCTCGGGCAGCTGACAGACAATGGAC
GGGCGGCTGAAGCAGGGCTAAAAGAAGGGGATTATATCCAAAGCATTAACGGAGAGAAAATGAGGTCTTGGACTGACATT
GTCTCCGCTGTAAAAGAAAACCCGGAGAAAGAAATGGACGTTGCAGTAAAAAGAGATAACAAAACGCTTCATATTTCGGT
GACTCCGGAAGCTGTTAAAGATGAGAACAAAAAAACAATCGGACGTTTCGGTTCCTATGCGCCGACTGAAAAAGGCGTAC
TCTCAGCGGTTGCTTACGGCGCGACATCAACAGTTGATGTCACCAAAGCCATTTTAACCAATCTGAGCAAATTAGTAACA
GGCCAATTTAAACTCGATATGCTGTCAGGTCCTGTCGGCATATATGACATGACAGACCAAGTGGCGAAAACAGGGATCGT
GAACTTATTTCAGTTTGCGGCGTTTTTAAGCATTAACCTTGGGATTGTCAACCTGCTTCCGATTCCGGCACTTGACGGAG
GAAGACTGTTGTTTCTATTTATTGAAGCGATTCGGGGCAAACCGATTAACAGGGAAAAAGAAGCATTTGTTGTGTTTATC
GGAGTAGCTTTCTTAATGCTTCTTATGCTGGTTGTCACATGGAACGATATCCAGCGGCTGTTTTTGTAA

Upstream 100 bases:

>100_bases
AACCCGCCATCAATTACTGAAAAAACCGAGCCTGGCGGACATTCAAGAAGTGGACAAAGATACCCGGGGATACGTCAATT
CAATACTCACATAAGGTGGT

Downstream 100 bases:

>100_bases
ACGAAAAGTAAATCAATCAGAGGTGCGAAGAAATGAGACAAAGCTTGACGCTTATTCCTACGCTCCGTGAAGTTCCAGCT
GATGCCGAAGCAAAAAGTCA

Product: inner membrane zinc metalloprotease

Products: NA

Alternate protein names: Regulating alternative sigma factor protease; Regulating anti-sigma-W factor activity protease

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MFVNTVIAFIIIFGTLVFFHELGHLLLAQRAGILCREFAIGFGPKIFSFKKNETVYTIRLLPVGGFVRMAGEDPEMIEVK
PGYTVGLLFNKEDQVEKVIINQKEKYPDALVIEVETADLEHDMKITGYEQGKEDELSSFTVSETSFFIVDGEEVQIAPYN
RQFGSKPVWQRIKAIAAGPIMNFILAYVILVMLGLIQGVPSNEPMLGQLTDNGRAAEAGLKEGDYIQSINGEKMRSWTDI
VSAVKENPEKEMDVAVKRDNKTLHISVTPEAVKDENKKTIGRFGSYAPTEKGVLSAVAYGATSTVDVTKAILTNLSKLVT
GQFKLDMLSGPVGIYDMTDQVAKTGIVNLFQFAAFLSINLGIVNLLPIPALDGGRLLFLFIEAIRGKPINREKEAFVVFI
GVAFLMLLMLVVTWNDIQRLFL

Sequences:

>Translated_422_residues
MFVNTVIAFIIIFGTLVFFHELGHLLLAQRAGILCREFAIGFGPKIFSFKKNETVYTIRLLPVGGFVRMAGEDPEMIEVK
PGYTVGLLFNKEDQVEKVIINQKEKYPDALVIEVETADLEHDMKITGYEQGKEDELSSFTVSETSFFIVDGEEVQIAPYN
RQFGSKPVWQRIKAIAAGPIMNFILAYVILVMLGLIQGVPSNEPMLGQLTDNGRAAEAGLKEGDYIQSINGEKMRSWTDI
VSAVKENPEKEMDVAVKRDNKTLHISVTPEAVKDENKKTIGRFGSYAPTEKGVLSAVAYGATSTVDVTKAILTNLSKLVT
GQFKLDMLSGPVGIYDMTDQVAKTGIVNLFQFAAFLSINLGIVNLLPIPALDGGRLLFLFIEAIRGKPINREKEAFVVFI
GVAFLMLLMLVVTWNDIQRLFL
>Mature_422_residues
MFVNTVIAFIIIFGTLVFFHELGHLLLAQRAGILCREFAIGFGPKIFSFKKNETVYTIRLLPVGGFVRMAGEDPEMIEVK
PGYTVGLLFNKEDQVEKVIINQKEKYPDALVIEVETADLEHDMKITGYEQGKEDELSSFTVSETSFFIVDGEEVQIAPYN
RQFGSKPVWQRIKAIAAGPIMNFILAYVILVMLGLIQGVPSNEPMLGQLTDNGRAAEAGLKEGDYIQSINGEKMRSWTDI
VSAVKENPEKEMDVAVKRDNKTLHISVTPEAVKDENKKTIGRFGSYAPTEKGVLSAVAYGATSTVDVTKAILTNLSKLVT
GQFKLDMLSGPVGIYDMTDQVAKTGIVNLFQFAAFLSINLGIVNLLPIPALDGGRLLFLFIEAIRGKPINREKEAFVVFI
GVAFLMLLMLVVTWNDIQRLFL

Specific function: Is responsible for Site-2 cleavage of the RsiW anti- sigma factor. This results, after a third proteolytic step catalyzed by the ClpXP protease, in the release of SigW and the transcription activation of the genes under the control of the sigma-W factor

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain

Homologues:

Organism=Escherichia coli, GI1786373, Length=239, Percent_Identity=36.4016736401674, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RASP_BACSU (O31754)

Other databases:

- EMBL:   AL009126
- PIR:   C69881
- RefSeq:   NP_389538.1
- ProteinModelPortal:   O31754
- MEROPS:   M50.004
- EnsemblBacteria:   EBBACT00000000544
- GeneID:   939612
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16560
- NMPDR:   fig|224308.1.peg.1659
- GenoList:   BSU16560
- GeneTree:   EBGT00050000001607
- HOGENOM:   HBG664087
- OMA:   HGTEFVV
- PhylomeDB:   O31754
- ProtClustDB:   CLSK2460922
- BioCyc:   BSUB:BSU16560-MONOMER
- GO:   GO:0006508
- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915
- SMART:   SM00228
- TIGRFAMs:   TIGR00054

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50; SSF50156 PDZ

EC number: NA

Molecular weight: Translated: 46744; Mature: 46744

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50106 PDZ; PS00142 ZINC_PROTEASE

Important sites: ACT_SITE 21-21

Signals:

None

Transmembrane regions:

HASH(0xf3b1cf0)-; HASH(0xe75c9c8)-; HASH(0xf404158)-; HASH(0xe71ed94)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVNTVIAFIIIFGTLVFFHELGHLLLAQRAGILCREFAIGFGPKIFSFKKNETVYTIRL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCEEEEEEE
LPVGGFVRMAGEDPEMIEVKPGYTVGLLFNKEDQVEKVIINQKEKYPDALVIEVETADLE
EECCCEEEECCCCCCEEEECCCEEEEEEECCCHHHHHHHHCCCCCCCCEEEEEEEECCCC
HDMKITGYEQGKEDELSSFTVSETSFFIVDGEEVQIAPYNRQFGSKPVWQRIKAIAAGPI
CCCEEECCCCCCCCCCCEEEEECCEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHH
MNFILAYVILVMLGLIQGVPSNEPMLGQLTDNGRAAEAGLKEGDYIQSINGEKMRSWTDI
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCCCEEECCCCHHHHHHHHH
VSAVKENPEKEMDVAVKRDNKTLHISVTPEAVKDENKKTIGRFGSYAPTEKGVLSAVAYG
HHHHHCCCCHHHCEEEEECCCEEEEEECHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHCC
ATSTVDVTKAILTNLSKLVTGQFKLDMLSGPVGIYDMTDQVAKTGIVNLFQFAAFLSINL
CCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHCC
GIVNLLPIPALDGGRLLFLFIEAIRGKPINREKEAFVVFIGVAFLMLLMLVVTWNDIQRL
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHH
FL
CH
>Mature Secondary Structure
MFVNTVIAFIIIFGTLVFFHELGHLLLAQRAGILCREFAIGFGPKIFSFKKNETVYTIRL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCEEEEEEE
LPVGGFVRMAGEDPEMIEVKPGYTVGLLFNKEDQVEKVIINQKEKYPDALVIEVETADLE
EECCCEEEECCCCCCEEEECCCEEEEEEECCCHHHHHHHHCCCCCCCCEEEEEEEECCCC
HDMKITGYEQGKEDELSSFTVSETSFFIVDGEEVQIAPYNRQFGSKPVWQRIKAIAAGPI
CCCEEECCCCCCCCCCCEEEEECCEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHH
MNFILAYVILVMLGLIQGVPSNEPMLGQLTDNGRAAEAGLKEGDYIQSINGEKMRSWTDI
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHCCCCCCCEEECCCCHHHHHHHHH
VSAVKENPEKEMDVAVKRDNKTLHISVTPEAVKDENKKTIGRFGSYAPTEKGVLSAVAYG
HHHHHCCCCHHHCEEEEECCCEEEEEECHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHCC
ATSTVDVTKAILTNLSKLVTGQFKLDMLSGPVGIYDMTDQVAKTGIVNLFQFAAFLSINL
CCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHCC
GIVNLLPIPALDGGRLLFLFIEAIRGKPINREKEAFVVFIGVAFLMLLMLVVTWNDIQRL
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHH
FL
CH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377