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The map label for this gene is proS

Identifier: 16078720

GI number: 16078720

Start: 1725330

End: 1727024

Strand: Direct

Name: proS

Synonym: BSU16570

Alternate gene names: 16078720

Gene position: 1725330-1727024 (Clockwise)

Preceding gene: 16078719

Following gene: 16078721

Centisome position: 40.93

GC content: 44.66

Gene sequence:

>1695_bases
ATGAGACAAAGCTTGACGCTTATTCCTACGCTCCGTGAAGTTCCAGCTGATGCCGAAGCAAAAAGTCATCAGCTTCTTCT
GAGAGCAGGATTTATCAGACAGAATACGAGCGGGGTATACAGCTATATGCCTCTTGCGTATAAGGTGATTCAAAACATTC
AGCAGATTGTTCGAGAGGAAATGGAGAAAATTGATGCCGTAGAAATGCTCATGCCCGCATTGCAGCAGGCAGAGACATGG
CAGGAATCAGGCAGATGGTATACGTATGGTCCCGAACTGATGAGACTAAAAGACCGTCATGGCCGTGAATTTGCTTTAGG
GGCAACGCATGAAGAGGTTATCACTTCGCTTGTTCGCGATGAGGTTAAATCTTATAAGCGTCTCCCTCTGACTCTTTATC
AAATTCAGTCTAAGTTCAGAGATGAAAAACGTCCTCGCTTCGGTTTGTTAAGAGGCCGCGAATTTATTATGAAGGATGCG
TACTCTTTCCATGCATCTGCAGAGAGCCTGGATGAAACGTATCAAAAAATGTACGAGGCCTATTCTAATATTTTTGCCCG
CTGCGGCATTAATGTAAGACCTGTTATCGCTGATTCAGGCGCAATGGGAGGAAAGGATACGCACGAATTTATGGCACTTT
CTGCGATCGGAGAGGATACGATTGCGTATTCTGATGAATCACAGTACGCGGCTAATATCGAGATGGCTGAAGTTCTTCAC
CAGGAAGTTCCTTCAGATGAAGAGCCTAAAGCTCTAGAGAAGGTTCATACGCCTAACGTGAAAACAATCGAAGAACTGAC
TGCGTTCTTACAGGTTTCGGCTGAAGCGTGCATTAAGTCAGTATTGTTTAAAGCTGATGACCGTTTTGTCTTAGTGCTTG
TAAGAGGGGATCATGAAGTTAACGATATTAAAGTGAAAAACTTGCTTCATGCAGAAGTTGTAGAGCTTGCTACACATGAA
GAGGTCATTCAGCAGCTCGGAACAGAGCCAGGCTTTGTAGGCCCTGTCGGTATTCATCAGGATGTGGAAGTATATGCCGA
TCAAGCTGTGAAAGCAATGGTTAATGCTGTTGCCGGGGCAAATGAAGGAGATCATCATTATAAAAATGTCAATGTGAATC
GTGACGCGCAAATTAAAGAATTTGCTGATCTTCGTTTTATTAAAGAAGGTGATCCTTCACCAGACGGCAAGGGAACGATC
CGTTTTGCTGAGGGAATCGAAGTCGGACAAGTCTTTAAGCTCGGAACACGCTATTCAGAAGCGATGAATGCGACATATTT
AGATGAAAACGGACGCGCGCAGCCAATGCTGATGGGCTGTTACGGAATCGGTGTGTCAAGAACGCTTTCTGCTATTGCTG
AACAGCATCACGACGAAAAAGGCTTAATATGGCCAAAAAGCGTTGCGCCGTACGATCTTCATATTCTTGCTTTGAACATG
AAAAACGATGGGCAAAGAGAGCTTGCTGAAAAGCTGTATGCCGATTTAAAAGCGGAAGGCTATGAAGTGCTCTATGATGA
CCGTGCTGAGCGTGCCGGCGTAAAATTCGCTGATTCAGATTTAATCGGCCTTCCAATCCGCATCACTGTCGGAAAACGAG
CTGACGAAGGAATCGTCGAAGTGAAAATTCGTCAAACTGGTGAGTCAACTGAGATTTCAGTAGACGAATTATCTGCGTTT
ATCAGCAAGCAGTAA

Upstream 100 bases:

>100_bases
GAGTAGCTTTCTTAATGCTTCTTATGCTGGTTGTCACATGGAACGATATCCAGCGGCTGTTTTTGTAAACGAAAAGTAAA
TCAATCAGAGGTGCGAAGAA

Downstream 100 bases:

>100_bases
TCACCATAACGATTTATCAAAAAACATGTTAAAATAGAAATAATAGAAGGTACCTCATTGCCTGAGGTACCTTCACTTAT
GATGTTTTTAGGGAGGGATA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MRQSLTLIPTLREVPADAEAKSHQLLLRAGFIRQNTSGVYSYMPLAYKVIQNIQQIVREEMEKIDAVEMLMPALQQAETW
QESGRWYTYGPELMRLKDRHGREFALGATHEEVITSLVRDEVKSYKRLPLTLYQIQSKFRDEKRPRFGLLRGREFIMKDA
YSFHASAESLDETYQKMYEAYSNIFARCGINVRPVIADSGAMGGKDTHEFMALSAIGEDTIAYSDESQYAANIEMAEVLH
QEVPSDEEPKALEKVHTPNVKTIEELTAFLQVSAEACIKSVLFKADDRFVLVLVRGDHEVNDIKVKNLLHAEVVELATHE
EVIQQLGTEPGFVGPVGIHQDVEVYADQAVKAMVNAVAGANEGDHHYKNVNVNRDAQIKEFADLRFIKEGDPSPDGKGTI
RFAEGIEVGQVFKLGTRYSEAMNATYLDENGRAQPMLMGCYGIGVSRTLSAIAEQHHDEKGLIWPKSVAPYDLHILALNM
KNDGQRELAEKLYADLKAEGYEVLYDDRAERAGVKFADSDLIGLPIRITVGKRADEGIVEVKIRQTGESTEISVDELSAF
ISKQ

Sequences:

>Translated_564_residues
MRQSLTLIPTLREVPADAEAKSHQLLLRAGFIRQNTSGVYSYMPLAYKVIQNIQQIVREEMEKIDAVEMLMPALQQAETW
QESGRWYTYGPELMRLKDRHGREFALGATHEEVITSLVRDEVKSYKRLPLTLYQIQSKFRDEKRPRFGLLRGREFIMKDA
YSFHASAESLDETYQKMYEAYSNIFARCGINVRPVIADSGAMGGKDTHEFMALSAIGEDTIAYSDESQYAANIEMAEVLH
QEVPSDEEPKALEKVHTPNVKTIEELTAFLQVSAEACIKSVLFKADDRFVLVLVRGDHEVNDIKVKNLLHAEVVELATHE
EVIQQLGTEPGFVGPVGIHQDVEVYADQAVKAMVNAVAGANEGDHHYKNVNVNRDAQIKEFADLRFIKEGDPSPDGKGTI
RFAEGIEVGQVFKLGTRYSEAMNATYLDENGRAQPMLMGCYGIGVSRTLSAIAEQHHDEKGLIWPKSVAPYDLHILALNM
KNDGQRELAEKLYADLKAEGYEVLYDDRAERAGVKFADSDLIGLPIRITVGKRADEGIVEVKIRQTGESTEISVDELSAF
ISKQ
>Mature_564_residues
MRQSLTLIPTLREVPADAEAKSHQLLLRAGFIRQNTSGVYSYMPLAYKVIQNIQQIVREEMEKIDAVEMLMPALQQAETW
QESGRWYTYGPELMRLKDRHGREFALGATHEEVITSLVRDEVKSYKRLPLTLYQIQSKFRDEKRPRFGLLRGREFIMKDA
YSFHASAESLDETYQKMYEAYSNIFARCGINVRPVIADSGAMGGKDTHEFMALSAIGEDTIAYSDESQYAANIEMAEVLH
QEVPSDEEPKALEKVHTPNVKTIEELTAFLQVSAEACIKSVLFKADDRFVLVLVRGDHEVNDIKVKNLLHAEVVELATHE
EVIQQLGTEPGFVGPVGIHQDVEVYADQAVKAMVNAVAGANEGDHHYKNVNVNRDAQIKEFADLRFIKEGDPSPDGKGTI
RFAEGIEVGQVFKLGTRYSEAMNATYLDENGRAQPMLMGCYGIGVSRTLSAIAEQHHDEKGLIWPKSVAPYDLHILALNM
KNDGQRELAEKLYADLKAEGYEVLYDDRAERAGVKFADSDLIGLPIRITVGKRADEGIVEVKIRQTGESTEISVDELSAF
ISKQ

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=253, Percent_Identity=41.1067193675889, Blast_Score=216, Evalue=4e-56,
Organism=Escherichia coli, GI1786392, Length=571, Percent_Identity=49.9124343257443, Blast_Score=566, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI115532348, Length=248, Percent_Identity=34.2741935483871, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=31.4606741573034, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=536, Percent_Identity=28.544776119403, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24656200, Length=219, Percent_Identity=34.2465753424658, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_BACSU (O31755)

Other databases:

- EMBL:   AL009126
- PIR:   G69682
- RefSeq:   NP_389539.1
- ProteinModelPortal:   O31755
- SMR:   O31755
- EnsemblBacteria:   EBBACT00000000387
- GeneID:   939617
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16570
- NMPDR:   fig|224308.1.peg.1660
- GenoList:   BSU16570
- GeneTree:   EBGT00050000001167
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   O31755
- ProtClustDB:   PRK09194
- BioCyc:   BSUB:BSU16570-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63306; Mature: 63306

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQSLTLIPTLREVPADAEAKSHQLLLRAGFIRQNTSGVYSYMPLAYKVIQNIQQIVREE
CCCCCEECCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MEKIDAVEMLMPALQQAETWQESGRWYTYGPELMRLKDRHGREFALGATHEEVITSLVRD
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCEEECCCCHHHHHHHHHHH
EVKSYKRLPLTLYQIQSKFRDEKRPRFGLLRGREFIMKDAYSFHASAESLDETYQKMYEA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YSNIFARCGINVRPVIADSGAMGGKDTHEFMALSAIGEDTIAYSDESQYAANIEMAEVLH
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEECCCCCHHHCHHHHHHHH
QEVPSDEEPKALEKVHTPNVKTIEELTAFLQVSAEACIKSVLFKADDRFVLVLVRGDHEV
HHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
NDIKVKNLLHAEVVELATHEEVIQQLGTEPGFVGPVGIHQDVEVYADQAVKAMVNAVAGA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
NEGDHHYKNVNVNRDAQIKEFADLRFIKEGDPSPDGKGTIRFAEGIEVGQVFKLGTRYSE
CCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
AMNATYLDENGRAQPMLMGCYGIGVSRTLSAIAEQHHDEKGLIWPKSVAPYDLHILALNM
HCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEC
KNDGQRELAEKLYADLKAEGYEVLYDDRAERAGVKFADSDLIGLPIRITVGKRADEGIVE
CCCCHHHHHHHHHHHHCCCCCEEEECCCHHHCCCEECCCCEEECEEEEEECCCCCCCEEE
VKIRQTGESTEISVDELSAFISKQ
EEEEECCCCCEEEHHHHHHHHCCC
>Mature Secondary Structure
MRQSLTLIPTLREVPADAEAKSHQLLLRAGFIRQNTSGVYSYMPLAYKVIQNIQQIVREE
CCCCCEECCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MEKIDAVEMLMPALQQAETWQESGRWYTYGPELMRLKDRHGREFALGATHEEVITSLVRD
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCEEECCCCHHHHHHHHHHH
EVKSYKRLPLTLYQIQSKFRDEKRPRFGLLRGREFIMKDAYSFHASAESLDETYQKMYEA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YSNIFARCGINVRPVIADSGAMGGKDTHEFMALSAIGEDTIAYSDESQYAANIEMAEVLH
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEECCCCCHHHCHHHHHHHH
QEVPSDEEPKALEKVHTPNVKTIEELTAFLQVSAEACIKSVLFKADDRFVLVLVRGDHEV
HHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
NDIKVKNLLHAEVVELATHEEVIQQLGTEPGFVGPVGIHQDVEVYADQAVKAMVNAVAGA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
NEGDHHYKNVNVNRDAQIKEFADLRFIKEGDPSPDGKGTIRFAEGIEVGQVFKLGTRYSE
CCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
AMNATYLDENGRAQPMLMGCYGIGVSRTLSAIAEQHHDEKGLIWPKSVAPYDLHILALNM
HCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEC
KNDGQRELAEKLYADLKAEGYEVLYDDRAERAGVKFADSDLIGLPIRITVGKRADEGIVE
CCCCHHHHHHHHHHHHCCCCCEEEECCCHHHCCCEECCCCEEECEEEEEECCCCCCCEEE
VKIRQTGESTEISVDELSAFISKQ
EEEEECCCCCEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377