Click here to switch to the map view.

The map label for this gene is fmt

Identifier: 16078636

GI number: 16078636

Start: 1646999

End: 1647952

Strand: Direct

Name: fmt

Synonym: BSU15730

Alternate gene names: 16078636

Gene position: 1646999-1647952 (Clockwise)

Preceding gene: 16078635

Following gene: 16078637

Centisome position: 39.07

GC content: 47.59

Gene sequence:

>954_bases
ATGACGAGAATCGTATTTATGGGAACGCCGGATTTTTCAGTTCCTGTTTTAAGAACCCTGATAGAGGACGGATATGAAGT
AGTGGGGGTCGTCACACAGCCGGACCGTCCGAAGGGCAGAAAAAAAGTACTGACACCTCCTCCGGTAAAGGAAGAGGCAT
TGCGCCACGGCATCCCGGTTCTTCAGCCGGAAAAAGTCAGATTGACAGAAGAAATTGAAAAAGTGCTGGCATTAAAGCCT
GACCTGATTGTAACGGCGGCATTCGGACAAATTTTGCCTAAAGAGCTTCTCGACAGTCCAAAATACGGCTGCATTAACGT
TCACGCATCCCTTTTGCCGGAACTGCGCGGCGGTGCTCCGATCCATTATTCCATTCTGCAGGGAAAGAAGAAAACCGGGA
TTACCATTATGTATATGGTAGAAAAATTGGATGCGGGCGATATGATCTCAAAAGTGGAAGTGGACATTGAAGAAACCGAC
AATGTCGGAACACTGCACGATAAATTAAGCGTTGCTGGCGCGAAGCTATTGTCTGAAACGGTTCCAAACGTTATTGCCGG
CAGCATATCACCTGAAAAGCAGGATGAAGAGAAAGCGACGTACGCGCCTAACATTAAAAGAGAGCAAGAGCTGCTCGACT
GGTCCAGAACGGGCGAAGAGCTTTACAACCAGATCCGCGGCTTAAATCCATGGCCTGTTGCTTATACGACTCTAAACGGA
CAAAACTTGAAAATATGGGCGTCGAAAAAAATAGCGGCACCAACAACAGCTGAGCCCGGCACAGTCGTTGCAGTTGAAAA
GGAAGGAATTATCGTTGCCACAGGTAACGAGACGGCTCTGTTATTAACCGAACTCCAGCCGGCAGGCAAAAAACGAATGA
AAGGCGAAGACTTTGTGCGAGGCGCTCATGTTGAAGCCGGTGATGTGTTAGGAGTAAACAATGAAAAAAACTAG

Upstream 100 bases:

>100_bases
GTGCAGCATGAAATGGACCACTTAGACGGTGTGCTGTTTACATCTAAAATAAGTAAATACTATACAGAAGATGAACTAGC
GGATATGGAAGGATGATTTG

Downstream 100 bases:

>100_bases
TGTTCGTGACATCGCCCTTGAGGCGCTGATCAAATTAGAACAAAACCAGGCATACAGCAACCTGCTGCTGAAATCGGTCA
TTAAATCAAATGAACTGAGT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTRIVFMGTPDFSVPVLRTLIEDGYEVVGVVTQPDRPKGRKKVLTPPPVKEEALRHGIPVLQPEKVRLTEEIEKVLALKP
DLIVTAAFGQILPKELLDSPKYGCINVHASLLPELRGGAPIHYSILQGKKKTGITIMYMVEKLDAGDMISKVEVDIEETD
NVGTLHDKLSVAGAKLLSETVPNVIAGSISPEKQDEEKATYAPNIKREQELLDWSRTGEELYNQIRGLNPWPVAYTTLNG
QNLKIWASKKIAAPTTAEPGTVVAVEKEGIIVATGNETALLLTELQPAGKKRMKGEDFVRGAHVEAGDVLGVNNEKN

Sequences:

>Translated_317_residues
MTRIVFMGTPDFSVPVLRTLIEDGYEVVGVVTQPDRPKGRKKVLTPPPVKEEALRHGIPVLQPEKVRLTEEIEKVLALKP
DLIVTAAFGQILPKELLDSPKYGCINVHASLLPELRGGAPIHYSILQGKKKTGITIMYMVEKLDAGDMISKVEVDIEETD
NVGTLHDKLSVAGAKLLSETVPNVIAGSISPEKQDEEKATYAPNIKREQELLDWSRTGEELYNQIRGLNPWPVAYTTLNG
QNLKIWASKKIAAPTTAEPGTVVAVEKEGIIVATGNETALLLTELQPAGKKRMKGEDFVRGAHVEAGDVLGVNNEKN
>Mature_316_residues
TRIVFMGTPDFSVPVLRTLIEDGYEVVGVVTQPDRPKGRKKVLTPPPVKEEALRHGIPVLQPEKVRLTEEIEKVLALKPD
LIVTAAFGQILPKELLDSPKYGCINVHASLLPELRGGAPIHYSILQGKKKTGITIMYMVEKLDAGDMISKVEVDIEETDN
VGTLHDKLSVAGAKLLSETVPNVIAGSISPEKQDEEKATYAPNIKREQELLDWSRTGEELYNQIRGLNPWPVAYTTLNGQ
NLKIWASKKIAAPTTAEPGTVVAVEKEGIIVATGNETALLLTELQPAGKKRMKGEDFVRGAHVEAGDVLGVNNEKN

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=317, Percent_Identity=29.6529968454259, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI238814322, Length=280, Percent_Identity=30, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI164663775, Length=236, Percent_Identity=30.5084745762712, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1789683, Length=297, Percent_Identity=46.1279461279461, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1788589, Length=277, Percent_Identity=30.6859205776173, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI133930964, Length=324, Percent_Identity=29.320987654321, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319458, Length=277, Percent_Identity=26.3537906137184, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550868, Length=320, Percent_Identity=28.75, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28571984, Length=228, Percent_Identity=31.5789473684211, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585660, Length=244, Percent_Identity=29.0983606557377, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_BACSU (P94463)

Other databases:

- EMBL:   Y10304
- EMBL:   Y13937
- EMBL:   AL009126
- PIR:   A69626
- RefSeq:   NP_389455.1
- ProteinModelPortal:   P94463
- SMR:   P94463
- EnsemblBacteria:   EBBACT00000000164
- GeneID:   936571
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15730
- NMPDR:   fig|224308.1.peg.1575
- GenoList:   BSU15730
- GeneTree:   EBGT00050000001190
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   P94463
- ProtClustDB:   CLSK887288
- BioCyc:   BSUB:BSU15730-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34634; Mature: 34503

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIVFMGTPDFSVPVLRTLIEDGYEVVGVVTQPDRPKGRKKVLTPPPVKEEALRHGIPV
CCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCC
LQPEKVRLTEEIEKVLALKPDLIVTAAFGQILPKELLDSPKYGCINVHASLLPELRGGAP
CCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCC
IHYSILQGKKKTGITIMYMVEKLDAGDMISKVEVDIEETDNVGTLHDKLSVAGAKLLSET
EEEHHHCCCCCCCEEEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
VPNVIAGSISPEKQDEEKATYAPNIKREQELLDWSRTGEELYNQIRGLNPWPVAYTTLNG
HHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
QNLKIWASKKIAAPTTAEPGTVVAVEKEGIIVATGNETALLLTELQPAGKKRMKGEDFVR
CEEEEEECCCCCCCCCCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCHHHCCCCHHHC
GAHVEAGDVLGVNNEKN
CCCCCCCCEEECCCCCC
>Mature Secondary Structure 
TRIVFMGTPDFSVPVLRTLIEDGYEVVGVVTQPDRPKGRKKVLTPPPVKEEALRHGIPV
CEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCC
LQPEKVRLTEEIEKVLALKPDLIVTAAFGQILPKELLDSPKYGCINVHASLLPELRGGAP
CCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCC
IHYSILQGKKKTGITIMYMVEKLDAGDMISKVEVDIEETDNVGTLHDKLSVAGAKLLSET
EEEHHHCCCCCCCEEEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
VPNVIAGSISPEKQDEEKATYAPNIKREQELLDWSRTGEELYNQIRGLNPWPVAYTTLNG
HHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
QNLKIWASKKIAAPTTAEPGTVVAVEKEGIIVATGNETALLLTELQPAGKKRMKGEDFVR
CEEEEEECCCCCCCCCCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCHHHCCCCHHHC
GAHVEAGDVLGVNNEKN
CCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9086272; 9534248; 9384377