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Identifier: 16078635

GI number: 16078635

Start: 1646512

End: 1646994

Strand: Direct

Name: def

Synonym: BSU15720

Alternate gene names: 16078635

Gene position: 1646512-1646994 (Clockwise)

Preceding gene: 16078634

Following gene: 16078636

Centisome position: 39.06

GC content: 44.72

Gene sequence:

>483_bases
TTGGCAGTAAAAAAGGTCGTCACACATCCTGCGGAGGTTTTGGAAACACCTGCGGAAACCGTGACTGTTTTTGATAAAAA
GCTAAAAAAACTGCTTGATGATATGTACGACACCATGCTTGAAATGGATGGTGTCGGACTGGCAGCGCCGCAAATCGGCA
TTTTAAAAAGAGCGGCCGTCGTAGAAATCGGGGATGACAGAGGGAGAATTGATCTCGTTAATCCTGAAATTTTAGAAAAA
AGCGGCGAGCAAACCGGAATTGAAGGATGCTTGAGCTTTCCTAACGTCTATGGTGATGTCACACGTGCCGATTATGTCAA
AGTGCGTGCGTTTAACCGTCAGGGAAAACCGTTCATTCTGGAAGCGCGAGGCTTTTTAGCAAGAGCCGTGCAGCATGAAA
TGGACCACTTAGACGGTGTGCTGTTTACATCTAAAATAAGTAAATACTATACAGAAGATGAACTAGCGGATATGGAAGGA
TGA

Upstream 100 bases:

>100_bases
AACATTATAAACGCGAAATTGAACAAAAACATGTAATGATTTCAATTGATATGAATCCTTATATGATGATGTAAGATCTC
ACGTCTTGGAGGGTAACAAA

Downstream 100 bases:

>100_bases
TTTGATGACGAGAATCGTATTTATGGGAACGCCGGATTTTTCAGTTCCTGTTTTAAGAACCCTGATAGAGGACGGATATG
AAGTAGTGGGGGTCGTCACA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MAVKKVVTHPAEVLETPAETVTVFDKKLKKLLDDMYDTMLEMDGVGLAAPQIGILKRAAVVEIGDDRGRIDLVNPEILEK
SGEQTGIEGCLSFPNVYGDVTRADYVKVRAFNRQGKPFILEARGFLARAVQHEMDHLDGVLFTSKISKYYTEDELADMEG

Sequences:

>Translated_160_residues
MAVKKVVTHPAEVLETPAETVTVFDKKLKKLLDDMYDTMLEMDGVGLAAPQIGILKRAAVVEIGDDRGRIDLVNPEILEK
SGEQTGIEGCLSFPNVYGDVTRADYVKVRAFNRQGKPFILEARGFLARAVQHEMDHLDGVLFTSKISKYYTEDELADMEG
>Mature_159_residues
AVKKVVTHPAEVLETPAETVTVFDKKLKKLLDDMYDTMLEMDGVGLAAPQIGILKRAAVVEIGDDRGRIDLVNPEILEKS
GEQTGIEGCLSFPNVYGDVTRADYVKVRAFNRQGKPFILEARGFLARAVQHEMDHLDGVLFTSKISKYYTEDELADMEG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=48.3221476510067, Blast_Score=132, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24645728, Length=152, Percent_Identity=29.6052631578947, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF1_BACSU (P94462)

Other databases:

- EMBL:   Y10304
- EMBL:   Y13937
- EMBL:   AL009126
- PIR:   F69613
- RefSeq:   NP_389454.1
- ProteinModelPortal:   P94462
- SMR:   P94462
- EnsemblBacteria:   EBBACT00000000395
- GeneID:   938819
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15720
- NMPDR:   fig|224308.1.peg.1574
- GenoList:   BSU15720
- GeneTree:   EBGT00050000001175
- HOGENOM:   HBG665227
- OMA:   EINSHIL
- PhylomeDB:   P94462
- ProtClustDB:   PRK12846
- BioCyc:   BSUB:BSU15720-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 17776; Mature: 17644

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKKVVTHPAEVLETPAETVTVFDKKLKKLLDDMYDTMLEMDGVGLAAPQIGILKRAAV
CCCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHE
VEIGDDRGRIDLVNPEILEKSGEQTGIEGCLSFPNVYGDVTRADYVKVRAFNRQGKPFIL
EEECCCCCEEEECCHHHHHHCCCCCCHHHHHHCCHHHCCCCHHHEEEEEEECCCCCEEEE
EARGFLARAVQHEMDHLDGVLFTSKISKYYTEDELADMEG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCC
>Mature Secondary Structure 
AVKKVVTHPAEVLETPAETVTVFDKKLKKLLDDMYDTMLEMDGVGLAAPQIGILKRAAV
CCHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHE
VEIGDDRGRIDLVNPEILEKSGEQTGIEGCLSFPNVYGDVTRADYVKVRAFNRQGKPFIL
EEECCCCCEEEECCHHHHHHCCCCCCHHHHHHCCHHHCCCCHHHEEEEEEECCCCCEEEE
EARGFLARAVQHEMDHLDGVLFTSKISKYYTEDELADMEG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9086272; 9534248; 9384377