Click here to switch to the map view.

The map label for this gene is rsmB

Identifier: 16078637

GI number: 16078637

Start: 1647939

End: 1649282

Strand: Direct

Name: rsmB

Synonym: BSU15740

Alternate gene names: 16078637

Gene position: 1647939-1649282 (Clockwise)

Preceding gene: 16078636

Following gene: 16078638

Centisome position: 39.09

GC content: 46.5

Gene sequence:

>1344_bases
ATGAAAAAAACTAGTGTTCGTGACATCGCCCTTGAGGCGCTGATCAAATTAGAACAAAACCAGGCATACAGCAACCTGCT
GCTGAAATCGGTCATTAAATCAAATGAACTGAGTGATCAGAACAGAGGGCTTTTGACTGAACTTGTCTATGGTACATTAC
AAAATAAGATCGCGCTTGATTATATGCTTAAACCGTTTATCAATAAGCCTCAAAAGGTAAAGCCGTGGGTGATTCAGCTT
CTTCGCCTATCCTTATATCAAATGGAGTATTTGGAGAAGATTCCAGACAGGGCAGCCATTCATGAAGCGGTTGAGATTGC
TAAAATCCGCGGACACAAAGGCATTGCTTCATTTGTCAACGGCGTGCTCCGTTCTATTCAGCGCGAAGGTGTTCCATCCT
TTGACGCGATCGAAGATCCTGTCCGCCGGCTGGCGACAGAAACAAGCCATCCGGAATGGCTTGTGAAAGAGTGGGCGGAT
GCGTATGGATTTGAAGCTGCGGAAAAGATTTGCCGCATCCATCTCATTCCGCCGAAACAGACGCTGCGTGTCAATCAAAT
GAAAGCAGACAGAGCTGAGCTGCTTGACCAAATGGCTGCTGAGGGAATCGAAGTTGAAAAAGGCGACCTCGCTGAAGATG
CTGTGAAGCTCTTAAAGGGAACAATTGCCGGAACTCATTTCTTCCAAAACGGCGAAGTTTCTATCCAGGATGAGAGCTCC
ATGCTCGTCGCCCGCGCCCTTGATCCTAAGTCAGATGAAACAGTGCTTGACGCGTGTGCGGCGCCTGGCGGAAAGTCGGC
TCATATCGCAGAATTAATGAAGAACAAGGGGAGCGTTACGTCGCTTGATCTTCACAAACATAAAGTGAAGCTGATCAAAG
AAGCGGCAGATCGGCTTGGCCTGACAATCATTCATGCGGAAACAATGGATGCTAGAAAAGCGGGGGAAACGTTTGAAAAC
GAACAGTTTGACCGAATATTGGTCGATGCCCCGTGCTCAGGTTTCGGTGTTATCAGAAGAAAGCCGGACATGAAATACAC
GAAAAAACCTGACGACAGCGCCCGTCTAGCTGAAATCCAGCTGTCGATCTTGAGGGAAATCGCACCATTAGTAAAAAAAG
GTGGCACTCTTGTCTACAGTACGTGTACAATGGACCGGACAGAAAATGATGAAGTAATACATGCGTTTATACAAGAACAT
CCTGATTTTGAACCCGATTTGTCTCTTGAAAAGCGGCTGCCTGAAAAGGTGAGACCCTTTGTTCGGGATGGAAGGCTTCA
AATCCTTCCTCATTATTTCGGAACAGATGGTTTCTTTATTTGCAGCATGAGAAAGAAGGGATAA

Upstream 100 bases:

>100_bases
TTATTAACCGAACTCCAGCCGGCAGGCAAAAAACGAATGAAAGGCGAAGACTTTGTGCGAGGCGCTCATGTTGAAGCCGG
TGATGTGTTAGGAGTAAACA

Downstream 100 bases:

>100_bases
GCAATGGCAGAACTTAATAAAACAAAAGTAAGAAAAGAATTGCGGACAGAGCGTCCGTCTATTTATTCTTTTGAATTAGA
TGAAATCAAACAATGGCTGA

Product: RNA-binding Sun protein

Products: NA

Alternate protein names: rRNA (cytosine-C(5)-)-methyltransferase rsmB

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKKTSVRDIALEALIKLEQNQAYSNLLLKSVIKSNELSDQNRGLLTELVYGTLQNKIALDYMLKPFINKPQKVKPWVIQL
LRLSLYQMEYLEKIPDRAAIHEAVEIAKIRGHKGIASFVNGVLRSIQREGVPSFDAIEDPVRRLATETSHPEWLVKEWAD
AYGFEAAEKICRIHLIPPKQTLRVNQMKADRAELLDQMAAEGIEVEKGDLAEDAVKLLKGTIAGTHFFQNGEVSIQDESS
MLVARALDPKSDETVLDACAAPGGKSAHIAELMKNKGSVTSLDLHKHKVKLIKEAADRLGLTIIHAETMDARKAGETFEN
EQFDRILVDAPCSGFGVIRRKPDMKYTKKPDDSARLAEIQLSILREIAPLVKKGGTLVYSTCTMDRTENDEVIHAFIQEH
PDFEPDLSLEKRLPEKVRPFVRDGRLQILPHYFGTDGFFICSMRKKG

Sequences:

>Translated_447_residues
MKKTSVRDIALEALIKLEQNQAYSNLLLKSVIKSNELSDQNRGLLTELVYGTLQNKIALDYMLKPFINKPQKVKPWVIQL
LRLSLYQMEYLEKIPDRAAIHEAVEIAKIRGHKGIASFVNGVLRSIQREGVPSFDAIEDPVRRLATETSHPEWLVKEWAD
AYGFEAAEKICRIHLIPPKQTLRVNQMKADRAELLDQMAAEGIEVEKGDLAEDAVKLLKGTIAGTHFFQNGEVSIQDESS
MLVARALDPKSDETVLDACAAPGGKSAHIAELMKNKGSVTSLDLHKHKVKLIKEAADRLGLTIIHAETMDARKAGETFEN
EQFDRILVDAPCSGFGVIRRKPDMKYTKKPDDSARLAEIQLSILREIAPLVKKGGTLVYSTCTMDRTENDEVIHAFIQEH
PDFEPDLSLEKRLPEKVRPFVRDGRLQILPHYFGTDGFFICSMRKKG
>Mature_447_residues
MKKTSVRDIALEALIKLEQNQAYSNLLLKSVIKSNELSDQNRGLLTELVYGTLQNKIALDYMLKPFINKPQKVKPWVIQL
LRLSLYQMEYLEKIPDRAAIHEAVEIAKIRGHKGIASFVNGVLRSIQREGVPSFDAIEDPVRRLATETSHPEWLVKEWAD
AYGFEAAEKICRIHLIPPKQTLRVNQMKADRAELLDQMAAEGIEVEKGDLAEDAVKLLKGTIAGTHFFQNGEVSIQDESS
MLVARALDPKSDETVLDACAAPGGKSAHIAELMKNKGSVTSLDLHKHKVKLIKEAADRLGLTIIHAETMDARKAGETFEN
EQFDRILVDAPCSGFGVIRRKPDMKYTKKPDDSARLAEIQLSILREIAPLVKKGGTLVYSTCTMDRTENDEVIHAFIQEH
PDFEPDLSLEKRLPEKVRPFVRDGRLQILPHYFGTDGFFICSMRKKG

Specific function: Unknown

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family

Homologues:

Organism=Homo sapiens, GI76150625, Length=353, Percent_Identity=31.4447592067989, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI76150623, Length=353, Percent_Identity=31.4447592067989, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI32698918, Length=178, Percent_Identity=41.0112359550562, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI11545785, Length=178, Percent_Identity=29.7752808988764, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI23199998, Length=249, Percent_Identity=27.3092369477912, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI270288818, Length=249, Percent_Identity=27.3092369477912, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI8922322, Length=249, Percent_Identity=27.3092369477912, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI270288816, Length=248, Percent_Identity=27.0161290322581, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI39995082, Length=278, Percent_Identity=25.1798561151079, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI301336155, Length=278, Percent_Identity=25.1798561151079, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI40316918, Length=180, Percent_Identity=28.3333333333333, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI2367212, Length=453, Percent_Identity=34.2163355408389, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI87081985, Length=283, Percent_Identity=32.1554770318021, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17536757, Length=338, Percent_Identity=29.585798816568, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI25143471, Length=179, Percent_Identity=32.4022346368715, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115534021, Length=165, Percent_Identity=31.5151515151515, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71998419, Length=217, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324268, Length=304, Percent_Identity=31.25, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6319447, Length=202, Percent_Identity=29.7029702970297, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI22024126, Length=295, Percent_Identity=29.1525423728814, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24668781, Length=172, Percent_Identity=36.6279069767442, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21356513, Length=172, Percent_Identity=36.6279069767442, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356579, Length=223, Percent_Identity=30.4932735426009, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355201, Length=277, Percent_Identity=25.2707581227437, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19920494, Length=193, Percent_Identity=32.1243523316062, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMB_BACSU (P94464)

Other databases:

- EMBL:   Y13937
- EMBL:   AL009126
- EMBL:   Y10304
- PIR:   E69878
- RefSeq:   NP_389456.1
- ProteinModelPortal:   P94464
- SMR:   P94464
- EnsemblBacteria:   EBBACT00000000603
- GeneID:   938106
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15740
- NMPDR:   fig|224308.1.peg.1576
- GenoList:   BSU15740
- GeneTree:   EBGT00070000032123
- HOGENOM:   HBG726909
- OMA:   SEYGLET
- PhylomeDB:   P94464
- ProtClustDB:   PRK14902
- BioCyc:   BSUB:BSU15740-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR00563

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: NA

Molecular weight: Translated: 50275; Mature: 50275

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: ACT_SITE 382-382 BINDING 283-283 BINDING 310-310 BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTSVRDIALEALIKLEQNQAYSNLLLKSVIKSNELSDQNRGLLTELVYGTLQNKIALD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
YMLKPFINKPQKVKPWVIQLLRLSLYQMEYLEKIPDRAAIHEAVEIAKIRGHKGIASFVN
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHH
GVLRSIQREGVPSFDAIEDPVRRLATETSHPEWLVKEWADAYGFEAAEKICRIHLIPPKQ
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHEEECCCCHH
TLRVNQMKADRAELLDQMAAEGIEVEKGDLAEDAVKLLKGTIAGTHFFQNGEVSIQDESS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCEEEEECCCC
MLVARALDPKSDETVLDACAAPGGKSAHIAELMKNKGSVTSLDLHKHKVKLIKEAADRLG
EEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHC
LTIIHAETMDARKAGETFENEQFDRILVDAPCSGFGVIRRKPDMKYTKKPDDSARLAEIQ
EEEEEECCHHHHHHCCCCCCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCHHHHHHH
LSILREIAPLVKKGGTLVYSTCTMDRTENDEVIHAFIQEHPDFEPDLSLEKRLPEKVRPF
HHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHH
VRDGRLQILPHYFGTDGFFICSMRKKG
HHCCCEEEECCEECCCCEEEEEECCCC
>Mature Secondary Structure
MKKTSVRDIALEALIKLEQNQAYSNLLLKSVIKSNELSDQNRGLLTELVYGTLQNKIALD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
YMLKPFINKPQKVKPWVIQLLRLSLYQMEYLEKIPDRAAIHEAVEIAKIRGHKGIASFVN
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHH
GVLRSIQREGVPSFDAIEDPVRRLATETSHPEWLVKEWADAYGFEAAEKICRIHLIPPKQ
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHEEECCCCHH
TLRVNQMKADRAELLDQMAAEGIEVEKGDLAEDAVKLLKGTIAGTHFFQNGEVSIQDESS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCEEEEECCCC
MLVARALDPKSDETVLDACAAPGGKSAHIAELMKNKGSVTSLDLHKHKVKLIKEAADRLG
EEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHC
LTIIHAETMDARKAGETFENEQFDRILVDAPCSGFGVIRRKPDMKYTKKPDDSARLAEIQ
EEEEEECCHHHHHHCCCCCCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCHHHHHHH
LSILREIAPLVKKGGTLVYSTCTMDRTENDEVIHAFIQEHPDFEPDLSLEKRLPEKVRPF
HHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHH
VRDGRLQILPHYFGTDGFFICSMRKKG
HHCCCEEEECCEECCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272