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The map label for this gene is priA

Identifier: 16078634

GI number: 16078634

Start: 1644068

End: 1646485

Strand: Direct

Name: priA

Synonym: BSU15710

Alternate gene names: 16078634

Gene position: 1644068-1646485 (Clockwise)

Preceding gene: 16078633

Following gene: 16078635

Centisome position: 39.0

GC content: 46.4

Gene sequence:

>2418_bases
ATGAATTTTGCAGAAGTCATCGTTGATGTCAGCACCAAAAATATAGACAGGCCTTTTGATTATAAAATCCCAGACCATCT
GAAGGGCATGATCAAAACGGGGATGCGGGTCATTGTTCCGTTTGGCCCCCGTAAGATTCAAGGGTTTGTGACAGCAGTCA
AAGAAGCATCTGACCTATCGGGAAAATCTGTCAAGGAAGTAGAGGATTTATTAGATCTTACACCTGTTCTGACAGAGGAG
CTTATGATTCTGTCATCATGGCTGTCAGACAAAACGCTGTCTTTTAAAATAACGGCGCTTCAAGCAATGCTGCCTGCCGC
CTTAAAGGCGAAATATGAAAAAGAATTAAAAATTGCACACGGAGCCGATCTTCCGCCGCAAGTGGAACGGCTGTTTTCTG
AAACAAAGACGCTGCTGTATTCAGATATACCCGATCATGAGACGCTGAAACTGATTCAAAGGCATGTTCAAAAAGGGGAC
ATTGACGTGACGTATAAAGTCGCGCAAAAAACCAATAAAAAAATGGTCAGGCATATTCAGGCAAATGCGAGTAAAGAAGA
GCTTGCTAAGCAGGCTGAGGGATTGTCCCGCCAAGCAGCAAAACAGCAGGCGATTCTTCACTTTCTCATCTCTGAGCCGG
AAGGTGTGAAAATTCCTGCGGCGGAATTGTGCAAAAAAACGGACACGAGCTCTGCAACCATCAAAACCCTTATCCAAAAA
GGGCTTTTGAAGGAAAGCTATGAAGAGGTTTACAGAGATCCCTATCAGGACAAAATGTTCAAAAAAACAGAGCCCCTGCC
GCTGACAGACGAACAGAGGGCTGCCTTCGAGCCCATACGCGAGACATTGGACAGCGATGAGCATAAAGTGTTCCTCCTTC
ACGGCGTGACAGGAAGCGGAAAAACCGAAATTTATCTGCAATCAATTGAAAAAGTACTGGCGAAAGGAAAAGAAGCCATC
GTCCTCGTACCGGAAATATCGCTGACGCCGCAAATGGTCAACCGCTTCAAGGGCCGCTTCGGCTCCCAGGTGGCAGTCAT
GCACAGCGGTTTATCCACAGGAGAAAAATATGACGAGTGGCGTAAAATCCACCGGAAGGAAGTACGGCTTGTCGTCGGTG
CCAGATCCGCCATTTTTGCTCCGTTTGAAAATCTCGGAATGATAATCATCGATGAAGAGCACGAGTCTTCCTATAAACAG
GAAGAAATGCCGCGCTATCATGCGAAAGAGGTGGCAATCAAGCGGGCTGAGCATCACAGCTGCCCGGTTGTATTAGGGAG
TGCTACGCCGACATTAGAATCATATGCTAGAGCGCAAAAGGGCGTATACGAGCTTCTTTCACTGAAGCACCGTGTTAACC
ATCGGGTGATGCCCGAGGTTTCGCTTGTCGATATGAGAGAGGAGCTTAGAAACGGCAATCGCTCGATGTTTTCAGTTGAA
TTGATGGAAAAGCTTGAAGAAACGATTGCCAAGGGTGAGCAGGCGGTGCTGTTTTTAAATAAGAGGGGCTACTCTTCCTT
TGTGATGTGCAGGGATTGCGGCTATGTCCCGCAATGCCCGCACTGCGACATTTCCATGACGTATCATCGTTACGGCCAAA
GGCTGAAATGCCATTATTGCGGACATGAAGAGCCTGTTCCGCACACTTGTCCTGAATGCGCAAGCGAGCATATCCGCTTT
TTCGGAACGGGAACACAGCGAGTGGAGGAAGAGCTGACAAAAGTGCTGCCAAGTGCGAGAGTGATTCGGATGGATGTTGA
CACGACATCACGGAAAGGCGCCCATGAAAAATTACTGTCAGCTTTCGGAGAAGGAAAAGCGGATATTCTTCTCGGAACGC
AAATGATCGCGAAAGGGCTTGATTTTCCGAATGTCACGCTTGTCGGAGTGTTAAGTGCTGATACAACACTTCATATTCCT
GATTTCAGATCGGCTGAAAAAACATTTCAGCTGTTAACGCAAGTCAGCGGCCGGGCAGGCAGGCATGAAAAGCCCGGCCA
TGTCATCATTCAAACGTATACACCGTCTCATTACAGTATCCAGCTGACGAAAACACACGATTATGAAACGTTCTATCAGC
ATGAAATGGCGCACAGGAGAGAGCAATCCTATCCGCCATACTACTATTTGGCTCTTGTGACTGTTTCTCATGAGGAAGTG
GCGAAAGCAGCGGTTACAGCTGAAAAAATCGCTCATTTTTTGAAAGCAAACTGCGGAGCTGACACGAAAATTCTCGGACC
AAGCGCGTCGCCGATAGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAAACAGGAAACCCAGTTAT
CCGCTTTATTGAAGAAGATACTGGAACATTATAAACGCGAAATTGAACAAAAACATGTAATGATTTCAATTGATATGAAT
CCTTATATGATGATGTAA

Upstream 100 bases:

>100_bases
ACGGGCATAAACGCGAACTCCCGATTATGTCTAAGCTCGATGTTTCTTTTGAAATCCTTCAGGAGATCGCAGCCCTCTCA
AAACAAACCGGAGAGCGCTC

Downstream 100 bases:

>100_bases
GATCTCACGTCTTGGAGGGTAACAAATTGGCAGTAAAAAAGGTCGTCACACATCCTGCGGAGGTTTTGGAAACACCTGCG
GAAACCGTGACTGTTTTTGA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y

Number of amino acids: Translated: 805; Mature: 805

Protein sequence:

>805_residues
MNFAEVIVDVSTKNIDRPFDYKIPDHLKGMIKTGMRVIVPFGPRKIQGFVTAVKEASDLSGKSVKEVEDLLDLTPVLTEE
LMILSSWLSDKTLSFKITALQAMLPAALKAKYEKELKIAHGADLPPQVERLFSETKTLLYSDIPDHETLKLIQRHVQKGD
IDVTYKVAQKTNKKMVRHIQANASKEELAKQAEGLSRQAAKQQAILHFLISEPEGVKIPAAELCKKTDTSSATIKTLIQK
GLLKESYEEVYRDPYQDKMFKKTEPLPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQ
EEMPRYHAKEVAIKRAEHHSCPVVLGSATPTLESYARAQKGVYELLSLKHRVNHRVMPEVSLVDMREELRNGNRSMFSVE
LMEKLEETIAKGEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPHTCPECASEHIRF
FGTGTQRVEEELTKVLPSARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIP
DFRSAEKTFQLLTQVSGRAGRHEKPGHVIIQTYTPSHYSIQLTKTHDYETFYQHEMAHRREQSYPPYYYLALVTVSHEEV
AKAAVTAEKIAHFLKANCGADTKILGPSASPIARIKDRYRYQCVIKYKQETQLSALLKKILEHYKREIEQKHVMISIDMN
PYMMM

Sequences:

>Translated_805_residues
MNFAEVIVDVSTKNIDRPFDYKIPDHLKGMIKTGMRVIVPFGPRKIQGFVTAVKEASDLSGKSVKEVEDLLDLTPVLTEE
LMILSSWLSDKTLSFKITALQAMLPAALKAKYEKELKIAHGADLPPQVERLFSETKTLLYSDIPDHETLKLIQRHVQKGD
IDVTYKVAQKTNKKMVRHIQANASKEELAKQAEGLSRQAAKQQAILHFLISEPEGVKIPAAELCKKTDTSSATIKTLIQK
GLLKESYEEVYRDPYQDKMFKKTEPLPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQ
EEMPRYHAKEVAIKRAEHHSCPVVLGSATPTLESYARAQKGVYELLSLKHRVNHRVMPEVSLVDMREELRNGNRSMFSVE
LMEKLEETIAKGEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPHTCPECASEHIRF
FGTGTQRVEEELTKVLPSARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIP
DFRSAEKTFQLLTQVSGRAGRHEKPGHVIIQTYTPSHYSIQLTKTHDYETFYQHEMAHRREQSYPPYYYLALVTVSHEEV
AKAAVTAEKIAHFLKANCGADTKILGPSASPIARIKDRYRYQCVIKYKQETQLSALLKKILEHYKREIEQKHVMISIDMN
PYMMM
>Mature_805_residues
MNFAEVIVDVSTKNIDRPFDYKIPDHLKGMIKTGMRVIVPFGPRKIQGFVTAVKEASDLSGKSVKEVEDLLDLTPVLTEE
LMILSSWLSDKTLSFKITALQAMLPAALKAKYEKELKIAHGADLPPQVERLFSETKTLLYSDIPDHETLKLIQRHVQKGD
IDVTYKVAQKTNKKMVRHIQANASKEELAKQAEGLSRQAAKQQAILHFLISEPEGVKIPAAELCKKTDTSSATIKTLIQK
GLLKESYEEVYRDPYQDKMFKKTEPLPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAI
VLVPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQ
EEMPRYHAKEVAIKRAEHHSCPVVLGSATPTLESYARAQKGVYELLSLKHRVNHRVMPEVSLVDMREELRNGNRSMFSVE
LMEKLEETIAKGEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPHTCPECASEHIRF
FGTGTQRVEEELTKVLPSARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIP
DFRSAEKTFQLLTQVSGRAGRHEKPGHVIIQTYTPSHYSIQLTKTHDYETFYQHEMAHRREQSYPPYYYLALVTVSHEEV
AKAAVTAEKIAHFLKANCGADTKILGPSASPIARIKDRYRYQCVIKYKQETQLSALLKKILEHYKREIEQKHVMISIDMN
PYMMM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI1790370, Length=575, Percent_Identity=38.0869565217391, Blast_Score=423, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIA_BACSU (P94461)

Other databases:

- EMBL:   Y13937
- EMBL:   AL009126
- EMBL:   Y10304
- PIR:   A69682
- RefSeq:   NP_389453.1
- ProteinModelPortal:   P94461
- EnsemblBacteria:   EBBACT00000001568
- GeneID:   938122
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15710
- NMPDR:   fig|224308.1.peg.1573
- GenoList:   BSU15710
- GeneTree:   EBGT00070000032146
- HOGENOM:   HBG725563
- OMA:   TEVYLHV
- PhylomeDB:   P94461
- ProtClustDB:   PRK05580
- BioCyc:   BSUB:BSU15710-MONOMER
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00595

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C

EC number: NA

Molecular weight: Translated: 91355; Mature: 91355

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFAEVIVDVSTKNIDRPFDYKIPDHLKGMIKTGMRVIVPFGPRKIQGFVTAVKEASDLS
CCHHHEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCC
GKSVKEVEDLLDLTPVLTEELMILSSWLSDKTLSFKITALQAMLPAALKAKYEKELKIAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
GADLPPQVERLFSETKTLLYSDIPDHETLKLIQRHVQKGDIDVTYKVAQKTNKKMVRHIQ
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
ANASKEELAKQAEGLSRQAAKQQAILHFLISEPEGVKIPAAELCKKTDTSSATIKTLIQK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHH
GLLKESYEEVYRDPYQDKMFKKTEPLPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSG
HHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
KTEIYLQSIEKVLAKGKEAIVLVPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEW
CHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEHHCCCCCCHHHHHH
RKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQEEMPRYHAKEVAIKRAEHHS
HHHHHHHHEEEEECCHHHHCCHHHCCEEEEECCHHHHHHHHHCCHHHHHHHHHHHHCCCC
CPVVLGSATPTLESYARAQKGVYELLSLKHRVNHRVMPEVSLVDMREELRNGNRSMFSVE
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEHHH
LMEKLEETIAKGEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYC
HHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCEEHHHHCCCCEEEEEC
GHEEPVPHTCPECASEHIRFFGTGTQRVEEELTKVLPSARVIRMDVDTTSRKGAHEKLLS
CCCCCCCCCCHHHHHHHEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
AFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDFRSAEKTFQLLTQVSGRAG
HHCCCCCEEEECHHHHHCCCCCCCEEEEEEEECCCEEECCCCCCHHHHHHHHHHHHCCCC
RHEKPGHVIIQTYTPSHYSIQLTKTHDYETFYQHEMAHRREQSYPPYYYLALVTVSHEEV
CCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHH
AKAAVTAEKIAHFLKANCGADTKILGPSASPIARIKDRYRYQCVIKYKQETQLSALLKKI
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHCCEEEEEEEECHHHHHHHHHHHH
LEHYKREIEQKHVMISIDMNPYMMM
HHHHHHHHHHCEEEEEECCCCEECC
>Mature Secondary Structure
MNFAEVIVDVSTKNIDRPFDYKIPDHLKGMIKTGMRVIVPFGPRKIQGFVTAVKEASDLS
CCHHHEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCC
GKSVKEVEDLLDLTPVLTEELMILSSWLSDKTLSFKITALQAMLPAALKAKYEKELKIAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
GADLPPQVERLFSETKTLLYSDIPDHETLKLIQRHVQKGDIDVTYKVAQKTNKKMVRHIQ
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
ANASKEELAKQAEGLSRQAAKQQAILHFLISEPEGVKIPAAELCKKTDTSSATIKTLIQK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHH
GLLKESYEEVYRDPYQDKMFKKTEPLPLTDEQRAAFEPIRETLDSDEHKVFLLHGVTGSG
HHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
KTEIYLQSIEKVLAKGKEAIVLVPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEW
CHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEHHCCCCCCHHHHHH
RKIHRKEVRLVVGARSAIFAPFENLGMIIIDEEHESSYKQEEMPRYHAKEVAIKRAEHHS
HHHHHHHHEEEEECCHHHHCCHHHCCEEEEECCHHHHHHHHHCCHHHHHHHHHHHHCCCC
CPVVLGSATPTLESYARAQKGVYELLSLKHRVNHRVMPEVSLVDMREELRNGNRSMFSVE
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEHHH
LMEKLEETIAKGEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYC
HHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCEEHHHHCCCCEEEEEC
GHEEPVPHTCPECASEHIRFFGTGTQRVEEELTKVLPSARVIRMDVDTTSRKGAHEKLLS
CCCCCCCCCCHHHHHHHEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
AFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDFRSAEKTFQLLTQVSGRAG
HHCCCCCEEEECHHHHHCCCCCCCEEEEEEEECCCEEECCCCCCHHHHHHHHHHHHCCCC
RHEKPGHVIIQTYTPSHYSIQLTKTHDYETFYQHEMAHRREQSYPPYYYLALVTVSHEEV
CCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHH
AKAAVTAEKIAHFLKANCGADTKILGPSASPIARIKDRYRYQCVIKYKQETQLSALLKKI
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHCCEEEEEEEECHHHHHHHHHHHH
LEHYKREIEQKHVMISIDMNPYMMM
HHHHHHHHHHCEEEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272