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The map label for this gene is coaBC

Identifier: 16078633

GI number: 16078633

Start: 1642851

End: 1644071

Strand: Direct

Name: coaBC

Synonym: BSU15700

Alternate gene names: 16078633

Gene position: 1642851-1644071 (Clockwise)

Preceding gene: 16078632

Following gene: 16078634

Centisome position: 38.97

GC content: 46.36

Gene sequence:

>1221_bases
TTGCTTAACAATCGAAATGTGTTACTTTGCGTGAGTGGAGGCATCGCTGTTTATAAAGCCTGTGCGTTAACGAGCAAGCT
GGTTCAGGCAGGAGCAAATGTCAAAGTGATTATGACTGAATCCGCTTGCCGATTCGTTTCACCGCTGACATTTCAGGCAT
TGAGCCGCCACGAAGTCTATACAGATACATTTAAAGAACAAAATCCAAGCGTCATTTCTCATATTGATGCCGCAGACTGG
GCCGACTTGATTATCGTAGCGCCGGCTACGGCTAATGTGATTGGAAAACTGGCAAACGGCATCGCTGATGATATGCTGAC
GACAACATTGTTGGCAGCAACGGCTCCCGTTTGGATCGCGCCGGCAATGAACGTTCATATGTATGACCACCCGGCAGTCA
AACGAAACATTTCTGTTCTTTATCAGGACGGTTATTGTTTTATTGAGCCAAGTGAGGGCTATTTAGCATGCGGTTACGTA
GGAAAAGGCAGATTGGAAGAGCCGGAAAACATCGTAAAGCTGGCCGAAAAACATTTTGCTGAAGAAACATCCGCTCCTTT
AGAAGGCAAGCATGTGGTCATTACAGCCGGCCCAACGCGGGAAGCGATTGATCCTGTCCGGTTTTTTACCAATAAATCGA
CGGGCAAAATGGGCTACGCACTGGCGGAGGCTGCTGTTCAGCTCGGCGCACGGGTAATCTTAATTTCTGGGCCTGTTTCA
CTTGATCAGCCTAAAGGCCTTGCTGAGTTTATCCCTGTTCAATCGGCTGCGGATATGCGCGAAGCTGTGCTCTCTGTGTA
TGACGCAAGCGATATAGTCATTAAAACAGCTGCGGTAGCCGATTTTACGCCGAAAACAGTATTTGACCATAAAATGAAAA
AACAAGACGGCGGCATGACCTTAGAACTGAAAAGAACAGTCGATATTTTGAAAGAATTGGGCGAGAAAAAGAAAGAGCAG
ATTTTGGTCGGATTTGCCGCCGAAACACAAGACATTGAACACTATGCACGTAAAAAGCTCGCCGCTAAAAACCTTGATTT
GATTGTGGCAAATGATGTGAAAGCAAATGGGGCCGGTTTTGGTGCTGATACGAATATCGTGACAATCTTTTTCAAAGACG
GGCATAAACGCGAACTCCCGATTATGTCTAAGCTCGATGTTTCTTTTGAAATCCTTCAGGAGATCGCAGCCCTCTCAAAA
CAAACCGGAGAGCGCTCATGA

Upstream 100 bases:

>100_bases
AAAAGGAAGACCGCGAATAGTAGCACAAGTAGCAACCTATATCATGTAGGTTGTTATTTTTTTCCGTGCGGTTTTGTAAA
GTGCAGAGGGGGAGAATTCA

Downstream 100 bases:

>100_bases
ATTTTGCAGAAGTCATCGTTGATGTCAGCACCAAAAATATAGACAGGCCTTTTGATTATAAAATCCCAGACCATCTGAAG
GGCATGATCAAAACGGGGAT

Product: Coenzyme A biosynthesis bifunctional phosphopantothenoylcysteine synthetase/decarboxylase

Products: NA

Alternate protein names: DNA/pantothenate metabolism flavoprotein; Phosphopantothenoylcysteine decarboxylase; PPCDC; CoaC; Phosphopantothenate--cysteine ligase; CoaB; PPC synthetase; PPCS; Phosphopantothenoylcysteine synthase

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MLNNRNVLLCVSGGIAVYKACALTSKLVQAGANVKVIMTESACRFVSPLTFQALSRHEVYTDTFKEQNPSVISHIDAADW
ADLIIVAPATANVIGKLANGIADDMLTTTLLAATAPVWIAPAMNVHMYDHPAVKRNISVLYQDGYCFIEPSEGYLACGYV
GKGRLEEPENIVKLAEKHFAEETSAPLEGKHVVITAGPTREAIDPVRFFTNKSTGKMGYALAEAAVQLGARVILISGPVS
LDQPKGLAEFIPVQSAADMREAVLSVYDASDIVIKTAAVADFTPKTVFDHKMKKQDGGMTLELKRTVDILKELGEKKKEQ
ILVGFAAETQDIEHYARKKLAAKNLDLIVANDVKANGAGFGADTNIVTIFFKDGHKRELPIMSKLDVSFEILQEIAALSK
QTGERS

Sequences:

>Translated_406_residues
MLNNRNVLLCVSGGIAVYKACALTSKLVQAGANVKVIMTESACRFVSPLTFQALSRHEVYTDTFKEQNPSVISHIDAADW
ADLIIVAPATANVIGKLANGIADDMLTTTLLAATAPVWIAPAMNVHMYDHPAVKRNISVLYQDGYCFIEPSEGYLACGYV
GKGRLEEPENIVKLAEKHFAEETSAPLEGKHVVITAGPTREAIDPVRFFTNKSTGKMGYALAEAAVQLGARVILISGPVS
LDQPKGLAEFIPVQSAADMREAVLSVYDASDIVIKTAAVADFTPKTVFDHKMKKQDGGMTLELKRTVDILKELGEKKKEQ
ILVGFAAETQDIEHYARKKLAAKNLDLIVANDVKANGAGFGADTNIVTIFFKDGHKRELPIMSKLDVSFEILQEIAALSK
QTGERS
>Mature_406_residues
MLNNRNVLLCVSGGIAVYKACALTSKLVQAGANVKVIMTESACRFVSPLTFQALSRHEVYTDTFKEQNPSVISHIDAADW
ADLIIVAPATANVIGKLANGIADDMLTTTLLAATAPVWIAPAMNVHMYDHPAVKRNISVLYQDGYCFIEPSEGYLACGYV
GKGRLEEPENIVKLAEKHFAEETSAPLEGKHVVITAGPTREAIDPVRFFTNKSTGKMGYALAEAAVQLGARVILISGPVS
LDQPKGLAEFIPVQSAADMREAVLSVYDASDIVIKTAAVADFTPKTVFDHKMKKQDGGMTLELKRTVDILKELGEKKKEQ
ILVGFAAETQDIEHYARKKLAAKNLDLIVANDVKANGAGFGADTNIVTIFFKDGHKRELPIMSKLDVSFEILQEIAALSK
QTGERS

Specific function: Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine

COG id: COG0452

COG function: function code H; Phosphopantothenoylcysteine synthetase/decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PPC synthetase family

Homologues:

Organism=Homo sapiens, GI71725351, Length=180, Percent_Identity=36.1111111111111, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI87082301, Length=408, Percent_Identity=41.1764705882353, Blast_Score=281, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI17560194, Length=183, Percent_Identity=34.4262295081967, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6322762, Length=194, Percent_Identity=32.4742268041237, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24657262, Length=183, Percent_Identity=32.2404371584699, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COABC_BACSU (O35033)

Other databases:

- EMBL:   Y13937
- EMBL:   AL009126
- PIR:   D69878
- RefSeq:   NP_389452.1
- HSSP:   Q96CD2
- ProteinModelPortal:   O35033
- EnsemblBacteria:   EBBACT00000003347
- GeneID:   938487
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15700
- NMPDR:   fig|224308.1.peg.1572
- GenoList:   BSU15700
- GeneTree:   EBGT00050000001176
- HOGENOM:   HBG523890
- OMA:   VTSGPTH
- PhylomeDB:   O35033
- ProtClustDB:   CLSK887287
- BioCyc:   BSUB:BSU15700-MONOMER
- InterPro:   IPR005252
- InterPro:   IPR007085
- InterPro:   IPR003382
- Gene3D:   G3DSA:3.40.50.10300
- Gene3D:   G3DSA:3.40.50.1950
- TIGRFAMs:   TIGR00521

Pfam domain/function: PF04127 DFP; PF02441 Flavoprotein; SSF102645 DNA/pantothenate-metab_flavo_C; SSF52507 Flavoprotein

EC number: =4.1.1.36; =6.3.2.5

Molecular weight: Translated: 43978; Mature: 43978

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: ACT_SITE 157-157 BINDING 124-124 BINDING 281-281 BINDING 291-291 BINDING 339-339 BINDING 343-343

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNNRNVLLCVSGGIAVYKACALTSKLVQAGANVKVIMTESACRFVSPLTFQALSRHEVY
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHHCCHHH
TDTFKEQNPSVISHIDAADWADLIIVAPATANVIGKLANGIADDMLTTTLLAATAPVWIA
HHHHHCCCCCHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
PAMNVHMYDHPAVKRNISVLYQDGYCFIEPSEGYLACGYVGKGRLEEPENIVKLAEKHFA
CCCEEEEECCCHHHCCEEEEEECCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
EETSAPLEGKHVVITAGPTREAIDPVRFFTNKSTGKMGYALAEAAVQLGARVILISGPVS
HHCCCCCCCCEEEEECCCCHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEECCCC
LDQPKGLAEFIPVQSAADMREAVLSVYDASDIVIKTAAVADFTPKTVFDHKMKKQDGGMT
CCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEE
LELKRTVDILKELGEKKKEQILVGFAAETQDIEHYARKKLAAKNLDLIVANDVKANGAGF
EEHHHHHHHHHHHCCHHHHEEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GADTNIVTIFFKDGHKRELPIMSKLDVSFEILQEIAALSKQTGERS
CCCCCEEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNNRNVLLCVSGGIAVYKACALTSKLVQAGANVKVIMTESACRFVSPLTFQALSRHEVY
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHHCCHHH
TDTFKEQNPSVISHIDAADWADLIIVAPATANVIGKLANGIADDMLTTTLLAATAPVWIA
HHHHHCCCCCHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
PAMNVHMYDHPAVKRNISVLYQDGYCFIEPSEGYLACGYVGKGRLEEPENIVKLAEKHFA
CCCEEEEECCCHHHCCEEEEEECCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
EETSAPLEGKHVVITAGPTREAIDPVRFFTNKSTGKMGYALAEAAVQLGARVILISGPVS
HHCCCCCCCCEEEEECCCCHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEECCCC
LDQPKGLAEFIPVQSAADMREAVLSVYDASDIVIKTAAVADFTPKTVFDHKMKKQDGGMT
CCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEE
LELKRTVDILKELGEKKKEQILVGFAAETQDIEHYARKKLAAKNLDLIVANDVKANGAGF
EEHHHHHHHHHHHCCHHHHEEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GADTNIVTIFFKDGHKRELPIMSKLDVSFEILQEIAALSKQTGERS
CCCCCEEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377