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The map label for this gene is fruA

Identifier: 16078504

GI number: 16078504

Start: 1509256

End: 1511163

Strand: Direct

Name: fruA

Synonym: BSU14400

Alternate gene names: 16078504

Gene position: 1509256-1511163 (Clockwise)

Preceding gene: 16078503

Following gene: 16078505

Centisome position: 35.8

GC content: 46.59

Gene sequence:

>1908_bases
GTGAAAATAACTGAGCTTTTAACGAAGCATACGATAAAGCTTAATATTGAGAGCAAAGAAAAAGAAAATGTTATTGACGA
AATGGTCACTGTTCTTGATAAGGCTGGAAAGTTAAATGACCGACAAGCCTATAAAGAAGCCATTTTAAATCGCGAAAGCC
AAAGCTCCACGGGTATCGGTGAAGGAATCGCTATCCCCCATGCGAAAACGGCAAGCGTTATCAACCCGGCGATCGCGTTC
GGACGTTCAAAAGACGGCGTCGATTATGAATCATTAGACGGCCAGCCGGCTCACTTAGTGTTCATGATCGCGGCGACTGA
AGGCGCAAACAACACTCACCTTGAAGCATTGTCAAGACTTTCAACGCTGTTAATGCGTGAAGAAATCCGTAAGCAGCTCC
TTGAAGCTGAGTCTGAAGATGCCATCATCGACATTATTAATCAGCACGATAAAGATGATGACGAAGAGGAAGAGGAAGAA
GAAGCGGCACCAGCACCTGCCGGAAAAGGGAAAATTTTAGCGGTTACTGCATGCCCGACAGGTATTGCCCATACATTCAT
GGCTGCGGATGCCCTTAAAGAAAAAGCGAAAGAGCTTGGTGTGGAAATTAAGGTCGAAACAAATGGGTCAAGCGGCATTA
AGCACAAGCTCACTGCCCAAGAAATCGAAGATGCACCTGCAATCATTGTCGCAGCGGACAAGCAGGTTGAAATGGAACGT
TTTAAAGGCAAGCGCGTACTTCAAGTTCCTGTAACGGCGGGTATCAGACGTCCGCAAGAGCTTATCGAAAAAGCGATGAA
TCAAGATGCGCCGATTTATCAAGGCAGCGGCGGCGGTTCAGCAGCTTCAAATGATGATGAAGAAGCGAAAGGCAAGTCTG
GAAGCGGTATCGGAAACACGTTTTATAAGCACCTGATGAGCGGTGTCAGCAACATGCTTCCGTTCGTAGTCGGCGGCGGT
ATTCTCGTTGCGATTTCATTCTTCTGGGGAATTCACTCTGCTGATCCGAATGATCCTAGCTACAACACGTTTGCAGCAGC
ATTAAACTTTATCGGCGGCGACAATGCATTAAAACTCATTGTTGCAGTTCTGGCCGGTTTCATTGCGATGAGTATTGCAG
ATCGTCCTGGTTTTGCGCCTGGTATGGTCGGCGGATTTATGGCAACTCAAGCGAATGCTGGATTCTTAGGCGGCTTAATT
GCCGGATTCCTTGCCGGTTATGTCGTGATTTTACTGAAAAAAGTATTTACGTTTATCCCTCAGTCACTTGATGGATTAAA
ACCTGTTTTGATTTACCCGCTGTTCGGTATTTTTATCACTGGCGTATTAATGCAATTTGTCGTCAATACACCTGTAGCGG
CATTTATGAATTTCTTAACAAACTGGCTTGAAAGCCTTGGTACTGGAAACCTTGTGTTAATGGGTATTATCTTGGGCGGC
ATGATGGCGATCGATATGGGCGGTCCGCTTAATAAAGCAGCCTTTACGTTCGGTATCGCCATGATCGATGCAGGCAACTA
TGCGCCTCATGCAGCAATCATGGCCGGCGGTATGGTTCCTCCGCTTGGTATCGCACTTGCAACAACCATTTTCAGAAACA
AATTCACTCAGCGTGACCGTGAAGCTGGTATTACATGCTATTTCATGGGAGCTGCTTTCGTAACAGAGGGAGCGATCCCA
TTTGCTGCGGCTGATCCGCTTCGCGTCATACCAGCTGCTGTAGTCGGTGCAGCTGTTGCCGGCGGATTAACTGAATTCTT
CCGAGTAACGCTTCCGGCGCCTCATGGAGGAGTATTCGTAGCTTTCATTACAAACCATCCGATGCTTTACCTTTTAAGTA
TCGTGATCGGTGCTGTCGTGATGGCAATTATCCTCGGTATCGTCAAAAAACCTGTTACAGAAAAATAA

Upstream 100 bases:

>100_bases
GCAGTGCTACCGCGTTCTCTGAAGAACTTGGAACAGAAGAATTTGTCCAGCAGCTTCTTCCTGAAGTTAAGGTCACACGT
CTATAGAGGAGGAAACACAA

Downstream 100 bases:

>100_bases
GAAAAAAAGCGTCCTTGGTTCGAAAGGACGCTTTTTCTTATCCGGCCGGCAGGGAGCGCGGTTAAGTTATGTATTTTATT
CTTTTTTATGATAAAGTAAA

Product: phosphotransferase system (PTS) fructose-specific enzyme IIABC component

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MKITELLTKHTIKLNIESKEKENVIDEMVTVLDKAGKLNDRQAYKEAILNRESQSSTGIGEGIAIPHAKTASVINPAIAF
GRSKDGVDYESLDGQPAHLVFMIAATEGANNTHLEALSRLSTLLMREEIRKQLLEAESEDAIIDIINQHDKDDDEEEEEE
EAAPAPAGKGKILAVTACPTGIAHTFMAADALKEKAKELGVEIKVETNGSSGIKHKLTAQEIEDAPAIIVAADKQVEMER
FKGKRVLQVPVTAGIRRPQELIEKAMNQDAPIYQGSGGGSAASNDDEEAKGKSGSGIGNTFYKHLMSGVSNMLPFVVGGG
ILVAISFFWGIHSADPNDPSYNTFAAALNFIGGDNALKLIVAVLAGFIAMSIADRPGFAPGMVGGFMATQANAGFLGGLI
AGFLAGYVVILLKKVFTFIPQSLDGLKPVLIYPLFGIFITGVLMQFVVNTPVAAFMNFLTNWLESLGTGNLVLMGIILGG
MMAIDMGGPLNKAAFTFGIAMIDAGNYAPHAAIMAGGMVPPLGIALATTIFRNKFTQRDREAGITCYFMGAAFVTEGAIP
FAAADPLRVIPAAVVGAAVAGGLTEFFRVTLPAPHGGVFVAFITNHPMLYLLSIVIGAVVMAIILGIVKKPVTEK

Sequences:

>Translated_635_residues
MKITELLTKHTIKLNIESKEKENVIDEMVTVLDKAGKLNDRQAYKEAILNRESQSSTGIGEGIAIPHAKTASVINPAIAF
GRSKDGVDYESLDGQPAHLVFMIAATEGANNTHLEALSRLSTLLMREEIRKQLLEAESEDAIIDIINQHDKDDDEEEEEE
EAAPAPAGKGKILAVTACPTGIAHTFMAADALKEKAKELGVEIKVETNGSSGIKHKLTAQEIEDAPAIIVAADKQVEMER
FKGKRVLQVPVTAGIRRPQELIEKAMNQDAPIYQGSGGGSAASNDDEEAKGKSGSGIGNTFYKHLMSGVSNMLPFVVGGG
ILVAISFFWGIHSADPNDPSYNTFAAALNFIGGDNALKLIVAVLAGFIAMSIADRPGFAPGMVGGFMATQANAGFLGGLI
AGFLAGYVVILLKKVFTFIPQSLDGLKPVLIYPLFGIFITGVLMQFVVNTPVAAFMNFLTNWLESLGTGNLVLMGIILGG
MMAIDMGGPLNKAAFTFGIAMIDAGNYAPHAAIMAGGMVPPLGIALATTIFRNKFTQRDREAGITCYFMGAAFVTEGAIP
FAAADPLRVIPAAVVGAAVAGGLTEFFRVTLPAPHGGVFVAFITNHPMLYLLSIVIGAVVMAIILGIVKKPVTEK
>Mature_635_residues
MKITELLTKHTIKLNIESKEKENVIDEMVTVLDKAGKLNDRQAYKEAILNRESQSSTGIGEGIAIPHAKTASVINPAIAF
GRSKDGVDYESLDGQPAHLVFMIAATEGANNTHLEALSRLSTLLMREEIRKQLLEAESEDAIIDIINQHDKDDDEEEEEE
EAAPAPAGKGKILAVTACPTGIAHTFMAADALKEKAKELGVEIKVETNGSSGIKHKLTAQEIEDAPAIIVAADKQVEMER
FKGKRVLQVPVTAGIRRPQELIEKAMNQDAPIYQGSGGGSAASNDDEEAKGKSGSGIGNTFYKHLMSGVSNMLPFVVGGG
ILVAISFFWGIHSADPNDPSYNTFAAALNFIGGDNALKLIVAVLAGFIAMSIADRPGFAPGMVGGFMATQANAGFLGGLI
AGFLAGYVVILLKKVFTFIPQSLDGLKPVLIYPLFGIFITGVLMQFVVNTPVAAFMNFLTNWLESLGTGNLVLMGIILGG
MMAIDMGGPLNKAAFTFGIAMIDAGNYAPHAAIMAGGMVPPLGIALATTIFRNKFTQRDREAGITCYFMGAAFVTEGAIP
FAAADPLRVIPAAVVGAAVAGGLTEFFRVTLPAPHGGVFVAFITNHPMLYLLSIVIGAVVMAIILGIVKKPVTEK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain

Homologues:

Organism=Escherichia coli, GI1788492, Length=468, Percent_Identity=44.6581196581197, Blast_Score=397, Evalue=1e-111,
Organism=Escherichia coli, GI1786951, Length=610, Percent_Identity=33.6065573770492, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI87082348, Length=472, Percent_Identity=31.5677966101695, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1790386, Length=285, Percent_Identity=43.1578947368421, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788729, Length=409, Percent_Identity=25.9168704156479, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790387, Length=95, Percent_Identity=44.2105263157895, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788730, Length=88, Percent_Identity=46.5909090909091, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI2367327, Length=142, Percent_Identity=26.056338028169, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789597, Length=156, Percent_Identity=27.5641025641026, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI48994992, Length=145, Percent_Identity=29.6551724137931, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTF3A_BACSU (P71012)

Other databases:

- EMBL:   AF012285
- EMBL:   AL009126
- PIR:   H69626
- RefSeq:   NP_389323.1
- PDB:   2R4Q
- PDBsum:   2R4Q
- ProteinModelPortal:   P71012
- EnsemblBacteria:   EBBACT00000001398
- GeneID:   938757
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14400
- NMPDR:   fig|224308.1.peg.1442
- GenoList:   BSU14400
- GeneTree:   EBGT00050000000031
- HOGENOM:   HBG704192
- OMA:   ADKQVEM
- PhylomeDB:   P71012
- ProtClustDB:   CLSK887234
- BioCyc:   BSUB:BSU14400-MONOMER
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327
- Gene3D:   G3DSA:3.40.930.10
- TIGRFAMs:   TIGR00829
- TIGRFAMs:   TIGR00848
- TIGRFAMs:   TIGR01427

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB; SSF55804 PTrfase/Anion_transptr

EC number: =2.7.1.69

Molecular weight: Translated: 67185; Mature: 67185

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS51094 PTS_EIIA_TYPE_2; PS00372 PTS_EIIA_TYPE_2_HIS; PS51099 PTS_EIIB_TYPE_2; PS51104 PTS_EIIC_TYPE_2

Important sites: ACT_SITE 67-67 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

HASH(0xdd76f0c)-; HASH(0xd08d4c0)-; HASH(0xe9622bc)-; HASH(0xedd4d40)-; HASH(0xe1bb930)-; HASH(0xee7efbc)-; HASH(0xe929a8c)-; HASH(0xec9c138)-; HASH(0xe703c2c)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITELLTKHTIKLNIESKEKENVIDEMVTVLDKAGKLNDRQAYKEAILNRESQSSTGIG
CCHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
EGIAIPHAKTASVINPAIAFGRSKDGVDYESLDGQPAHLVFMIAATEGANNTHLEALSRL
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHH
STLLMREEIRKQLLEAESEDAIIDIINQHDKDDDEEEEEEEAAPAPAGKGKILAVTACPT
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEEEECCC
GIAHTFMAADALKEKAKELGVEIKVETNGSSGIKHKLTAQEIEDAPAIIVAADKQVEMER
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCCCEEEEECCCHHHHHH
FKGKRVLQVPVTAGIRRPQELIEKAMNQDAPIYQGSGGGSAASNDDEEAKGKSGSGIGNT
HCCCEEEEECHHHCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCHHH
FYKHLMSGVSNMLPFVVGGGILVAISFFWGIHSADPNDPSYNTFAAALNFIGGDNALKLI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
VAVLAGFIAMSIADRPGFAPGMVGGFMATQANAGFLGGLIAGFLAGYVVILLKKVFTFIP
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
QSLDGLKPVLIYPLFGIFITGVLMQFVVNTPVAAFMNFLTNWLESLGTGNLVLMGIILGG
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
MMAIDMGGPLNKAAFTFGIAMIDAGNYAPHAAIMAGGMVPPLGIALATTIFRNKFTQRDR
HHEEECCCCCCHHHHHHHHHEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
EAGITCYFMGAAFVTEGAIPFAAADPLRVIPAAVVGAAVAGGLTEFFRVTLPAPHGGVFV
HCCEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEE
AFITNHPMLYLLSIVIGAVVMAIILGIVKKPVTEK
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MKITELLTKHTIKLNIESKEKENVIDEMVTVLDKAGKLNDRQAYKEAILNRESQSSTGIG
CCHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
EGIAIPHAKTASVINPAIAFGRSKDGVDYESLDGQPAHLVFMIAATEGANNTHLEALSRL
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHH
STLLMREEIRKQLLEAESEDAIIDIINQHDKDDDEEEEEEEAAPAPAGKGKILAVTACPT
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEEEECCC
GIAHTFMAADALKEKAKELGVEIKVETNGSSGIKHKLTAQEIEDAPAIIVAADKQVEMER
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCCCEEEEECCCHHHHHH
FKGKRVLQVPVTAGIRRPQELIEKAMNQDAPIYQGSGGGSAASNDDEEAKGKSGSGIGNT
HCCCEEEEECHHHCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCCCCCCCHHH
FYKHLMSGVSNMLPFVVGGGILVAISFFWGIHSADPNDPSYNTFAAALNFIGGDNALKLI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
VAVLAGFIAMSIADRPGFAPGMVGGFMATQANAGFLGGLIAGFLAGYVVILLKKVFTFIP
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
QSLDGLKPVLIYPLFGIFITGVLMQFVVNTPVAAFMNFLTNWLESLGTGNLVLMGIILGG
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
MMAIDMGGPLNKAAFTFGIAMIDAGNYAPHAAIMAGGMVPPLGIALATTIFRNKFTQRDR
HHEEECCCCCCHHHHHHHHHEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
EAGITCYFMGAAFVTEGAIPFAAADPLRVIPAAVVGAAVAGGLTEFFRVTLPAPHGGVFV
HCCEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEE
AFITNHPMLYLLSIVIGAVVMAIILGIVKKPVTEK
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377