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The map label for this gene is yitF

Identifier: 16078161

GI number: 16078161

Start: 1174861

End: 1175976

Strand: Reverse

Name: yitF

Synonym: BSU10970

Alternate gene names: 16078161

Gene position: 1175976-1174861 (Counterclockwise)

Preceding gene: 16078162

Following gene: 16078160

Centisome position: 27.9

GC content: 50.27

Gene sequence:

>1116_bases
GTGAAAATTGTACGGATTGAAACCTTTCCGCTATTTCATCGATTAGAAAAGCCATACGGAGATGCCAATGGGTTTAAACG
ATATCGGACTTGTTATCTCATCAGGATTATCACCGAAAGCGGGATAGACGGCTGGGGAGAATGCGTTGATTGGCTCCCTG
CTCTCCATGTCGGTTTTACGAAGCGGATCATCCCATTTCTATTAGGAAAACAGGCTGGCAGCCGCCTGTCATTAGTGCGC
ACGATTCAAAAATGGCACCAGCGGGCGGCCTCCGCTGTAAGCATGGCGCTGACAGAGATTGCAGCCAAAGCTGCGGATTG
TTCGGTTTGCGAATTATGGGGCGGGCGATACAGAGAGGAGATTCCTGTGTACGCGTCTTTTCAATCGTACTCAGACTCCC
CGCAATGGATCAGCCGCTCAGTCTCCAATGTTGAAGCCCAGTTGAAAAAAGGTTTTGAGCAAATCAAAGTCAAAATTGGC
GGGACTTCATTCAAGGAGGATGTCCGGCACATCAATGCGCTGCAGCATACAGCAGGCAGCTCCATTACGATGATTCTGGA
CGCAAACCAAAGCTACGATGCCGCTGCGGCTTTTAAATGGGAGCGCTATTTCTCCGAATGGACGAACATTGGCTGGCTTG
AGGAGCCTTTGCCCTTTGATCAGCCGCAGGATTACGCTATGCTTCGAAGCCGTTTGTCTGTTCCTGTTGCCGGCGGAGAA
AACATGAAAGGCCCGGCGCAATATGTGCCTCTCCTTTCACAGCGCTGTTTGGATATCATTCAGCCCGATGTCATGCATGT
CAACGGAATTGATGAGTTTCGGGACTGCCTCCAGCTCGCGCGTTATTTCGGCGTCAGAGCATCCGCCCATGCATATGACG
GATCGCTCTCACGGCTCTATGCTTTATTTGCACAAGCCTGTCTGCCCCCATGGTCTAAAATGAAGAACGATCACATTGAA
CCGATCGAGTGGGACGTCATGGAGAATCCTTTTACAGATCTCGTCAGCCTGCAGCCTTCAAAAGGAATGGTCCACATCCC
GAAAGGAAAAGGCATTGGCACAGAGATCAATATGGAGATCGTCAATCGCTACAAGTGGGATGGCTCAGCCTATTAA

Upstream 100 bases:

>100_bases
TCATCCATGTGAAGCAAAACAATGAAGAAACAAAAGAAAAAGAAGACCCTAAAATGGCTGGCAATCGGCTCCAGCCCGCT
GAGGAAAGGTAGCACCCAGT

Downstream 100 bases:

>100_bases
GCTGGGCCATTTCTTTATTTTGAGACATAAATAATGGACAGCCTCATAAAATGAAAACAGCGGACAAGTCTTTTCATAAA
GGAGCAAATGACTTAATGGC

Product: enolase superfamily enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKIVRIETFPLFHRLEKPYGDANGFKRYRTCYLIRIITESGIDGWGECVDWLPALHVGFTKRIIPFLLGKQAGSRLSLVR
TIQKWHQRAASAVSMALTEIAAKAADCSVCELWGGRYREEIPVYASFQSYSDSPQWISRSVSNVEAQLKKGFEQIKVKIG
GTSFKEDVRHINALQHTAGSSITMILDANQSYDAAAAFKWERYFSEWTNIGWLEEPLPFDQPQDYAMLRSRLSVPVAGGE
NMKGPAQYVPLLSQRCLDIIQPDVMHVNGIDEFRDCLQLARYFGVRASAHAYDGSLSRLYALFAQACLPPWSKMKNDHIE
PIEWDVMENPFTDLVSLQPSKGMVHIPKGKGIGTEINMEIVNRYKWDGSAY

Sequences:

>Translated_371_residues
MKIVRIETFPLFHRLEKPYGDANGFKRYRTCYLIRIITESGIDGWGECVDWLPALHVGFTKRIIPFLLGKQAGSRLSLVR
TIQKWHQRAASAVSMALTEIAAKAADCSVCELWGGRYREEIPVYASFQSYSDSPQWISRSVSNVEAQLKKGFEQIKVKIG
GTSFKEDVRHINALQHTAGSSITMILDANQSYDAAAAFKWERYFSEWTNIGWLEEPLPFDQPQDYAMLRSRLSVPVAGGE
NMKGPAQYVPLLSQRCLDIIQPDVMHVNGIDEFRDCLQLARYFGVRASAHAYDGSLSRLYALFAQACLPPWSKMKNDHIE
PIEWDVMENPFTDLVSLQPSKGMVHIPKGKGIGTEINMEIVNRYKWDGSAY
>Mature_371_residues
MKIVRIETFPLFHRLEKPYGDANGFKRYRTCYLIRIITESGIDGWGECVDWLPALHVGFTKRIIPFLLGKQAGSRLSLVR
TIQKWHQRAASAVSMALTEIAAKAADCSVCELWGGRYREEIPVYASFQSYSDSPQWISRSVSNVEAQLKKGFEQIKVKIG
GTSFKEDVRHINALQHTAGSSITMILDANQSYDAAAAFKWERYFSEWTNIGWLEEPLPFDQPQDYAMLRSRLSVPVAGGE
NMKGPAQYVPLLSQRCLDIIQPDVMHVNGIDEFRDCLQLARYFGVRASAHAYDGSLSRLYALFAQACLPPWSKMKNDHIE
PIEWDVMENPFTDLVSLQPSKGMVHIPKGKGIGTEINMEIVNRYKWDGSAY

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family

Homologues:

Organism=Homo sapiens, GI42544119, Length=267, Percent_Identity=25.0936329588015, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI186972148, Length=267, Percent_Identity=25.0936329588015, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YITF_BACSU (O06741)

Other databases:

- EMBL:   Y09476
- EMBL:   AL009126
- PIR:   G69839
- RefSeq:   NP_388978.1
- PDB:   2GDQ
- PDB:   2GGE
- PDBsum:   2GDQ
- PDBsum:   2GGE
- ProteinModelPortal:   O06741
- SMR:   O06741
- EnsemblBacteria:   EBBACT00000001515
- GeneID:   939345
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10970
- NMPDR:   fig|224308.1.peg.1097
- GenoList:   BSU10970
- GeneTree:   EBGT00070000032382
- HOGENOM:   HBG348922
- OMA:   EWDVMEN
- ProtClustDB:   CLSK237674
- BioCyc:   BSUB:BSU10970-MONOMER
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- PANTHER:   PTHR13794:SF2
- PANTHER:   PTHR13794
- SMART:   SM00922

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N

EC number: NA

Molecular weight: Translated: 42053; Mature: 42053

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00908 MR_MLE_1; PS00909 MR_MLE_2

Important sites: ACT_SITE 158-158 ACT_SITE 290-290

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVRIETFPLFHRLEKPYGDANGFKRYRTCYLIRIITESGIDGWGECVDWLPALHVGFT
CEEEEEECCHHHHHHHCCCCCCCHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
KRIIPFLLGKQAGSRLSLVRTIQKWHQRAASAVSMALTEIAAKAADCSVCELWGGRYREE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHC
IPVYASFQSYSDSPQWISRSVSNVEAQLKKGFEQIKVKIGGTSFKEDVRHINALQHTAGS
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHEEEEECCCCHHHHHHHHHHHHHCCCC
SITMILDANQSYDAAAAFKWERYFSEWTNIGWLEEPLPFDQPQDYAMLRSRLSVPVAGGE
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
NMKGPAQYVPLLSQRCLDIIQPDVMHVNGIDEFRDCLQLARYFGVRASAHAYDGSLSRLY
CCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
ALFAQACLPPWSKMKNDHIEPIEWDVMENPFTDLVSLQPSKGMVHIPKGKGIGTEINMEI
HHHHHHHCCCHHHHCCCCCCCCEECCCCCCHHHHHCCCCCCCEEECCCCCCCCCEEEHHH
VNRYKWDGSAY
HHHCCCCCCCC
>Mature Secondary Structure
MKIVRIETFPLFHRLEKPYGDANGFKRYRTCYLIRIITESGIDGWGECVDWLPALHVGFT
CEEEEEECCHHHHHHHCCCCCCCHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
KRIIPFLLGKQAGSRLSLVRTIQKWHQRAASAVSMALTEIAAKAADCSVCELWGGRYREE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHC
IPVYASFQSYSDSPQWISRSVSNVEAQLKKGFEQIKVKIGGTSFKEDVRHINALQHTAGS
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHEEEEECCCCHHHHHHHHHHHHHCCCC
SITMILDANQSYDAAAAFKWERYFSEWTNIGWLEEPLPFDQPQDYAMLRSRLSVPVAGGE
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
NMKGPAQYVPLLSQRCLDIIQPDVMHVNGIDEFRDCLQLARYFGVRASAHAYDGSLSRLY
CCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
ALFAQACLPPWSKMKNDHIEPIEWDVMENPFTDLVSLQPSKGMVHIPKGKGIGTEINMEI
HHHHHHHCCCHHHHCCCCCCCCEECCCCCCHHHHHCCCCCCCEEECCCCCCCCCEEEHHH
VNRYKWDGSAY
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377