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The map label for this gene is acoB

Identifier: 16077874

GI number: 16077874

Start: 880007

End: 881035

Strand: Direct

Name: acoB

Synonym: BSU08070

Alternate gene names: 16077874

Gene position: 880007-881035 (Clockwise)

Preceding gene: 16077873

Following gene: 16077875

Centisome position: 20.87

GC content: 50.44

Gene sequence:

>1029_bases
ATGGCGAGAGTCATAAGCATGTCAGACGCGATCAATGAAGCAATGAAGCTTGCGATGAGAAAAGACGAAAATGTGCTTTT
GATCGGTGAGGATGTCGCCGGGGGAGCGGCGGTCGATCATTTGCAGGATGATGAAGCATGGGGCGGTGTATTAGGGGTCA
CAAAGGGACTCGTACAGGAATTCGGGCGTACAAGAGTGCTGGACACTCCGATTTCTGAGGCAGGCTATATGGGAGCGGCT
ATGGCTGCGGCATCAACCGGTTTGAGACCGATTGCCGAGCTGATGTTTAACGATTTTATCGGCACGTGCTTTGATCAGGT
GATCAACCAAGGGGCGAAATTCCGTTATATGTTCGGCGGAAAAGCGCAAGTGCCGATTACCGTCCGCACCACATACGGAG
CAGGGTTCCGGGCCGCTGCCCAGCATTCACAATCGCTGTATGGCCTTTTCACGAGCATCCCTGGACTGAAGACAGTTGTT
CCATCCAATCCGTATGATGCCAAAGGTCTTTTGCTTGCAGCAATAGAAGATAATGATCCGGTGTTTTTCTTTGAAGACAA
AACGTCCTACAACATGAAGGGCGAGGTGCCGGAAGATTATTATACAATTCCCCTCGGAAAAGCGGATATCAAACGCGAAG
GCAATGATGTTACGCTCTTTGCAGTCGGCAAGCAGGTCAATACTGCGCTTGAAGCGGCTGCACAGCTTTCAGAGAGGGGC
ATCGAAGCCGAGGTCCTTGATCCCCGCAGTCTGTCTCCTCTGGATGAGGATGCGATTTTCACATCGTTAGAAAAAACAAA
CCGGCTGATCATCATTGATGAAGCCAATCCGCGATGCAGCATTGCCACGGATATTGCTGCGCTTGTCGCTGACAAGGGCT
TTGATTTGCTTGATGCGCCGATTAAACGGATTACAGCGCCGCATACACCGGTTCCGTTTTCACCAGTGCTTGAAGATCAA
TATTTGCCGACACCAGATAAAATTGTCAGCGTCACGCTTGAATTGCTTGGCGAGCCGGCATTGAATTAA

Upstream 100 bases:

>100_bases
TGAAAAAGCCGTCTCGTTCAGCGAAGACAGCCCATATCCAAAAGATTCGGAGCTGCTGACAGATGTGTATGTGTCATATG
AAAAAGGAGGAATGTAAGAG

Downstream 100 bases:

>100_bases
AGGGGGGACACAAATGGCGGTAAAAGTAGTGATGCCAAAATTGGGAATGGCCATGAAACAAGGGGAAGTATCGATATGGA
ATAAAAAAGTAGGCGACCCG

Product: acetoin dehydrogenase E1 component (TPP-dependent beta subunit)

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MARVISMSDAINEAMKLAMRKDENVLLIGEDVAGGAAVDHLQDDEAWGGVLGVTKGLVQEFGRTRVLDTPISEAGYMGAA
MAAASTGLRPIAELMFNDFIGTCFDQVINQGAKFRYMFGGKAQVPITVRTTYGAGFRAAAQHSQSLYGLFTSIPGLKTVV
PSNPYDAKGLLLAAIEDNDPVFFFEDKTSYNMKGEVPEDYYTIPLGKADIKREGNDVTLFAVGKQVNTALEAAAQLSERG
IEAEVLDPRSLSPLDEDAIFTSLEKTNRLIIIDEANPRCSIATDIAALVADKGFDLLDAPIKRITAPHTPVPFSPVLEDQ
YLPTPDKIVSVTLELLGEPALN

Sequences:

>Translated_342_residues
MARVISMSDAINEAMKLAMRKDENVLLIGEDVAGGAAVDHLQDDEAWGGVLGVTKGLVQEFGRTRVLDTPISEAGYMGAA
MAAASTGLRPIAELMFNDFIGTCFDQVINQGAKFRYMFGGKAQVPITVRTTYGAGFRAAAQHSQSLYGLFTSIPGLKTVV
PSNPYDAKGLLLAAIEDNDPVFFFEDKTSYNMKGEVPEDYYTIPLGKADIKREGNDVTLFAVGKQVNTALEAAAQLSERG
IEAEVLDPRSLSPLDEDAIFTSLEKTNRLIIIDEANPRCSIATDIAALVADKGFDLLDAPIKRITAPHTPVPFSPVLEDQ
YLPTPDKIVSVTLELLGEPALN
>Mature_341_residues
ARVISMSDAINEAMKLAMRKDENVLLIGEDVAGGAAVDHLQDDEAWGGVLGVTKGLVQEFGRTRVLDTPISEAGYMGAAM
AAASTGLRPIAELMFNDFIGTCFDQVINQGAKFRYMFGGKAQVPITVRTTYGAGFRAAAQHSQSLYGLFTSIPGLKTVVP
SNPYDAKGLLLAAIEDNDPVFFFEDKTSYNMKGEVPEDYYTIPLGKADIKREGNDVTLFAVGKQVNTALEAAAQLSERGI
EAEVLDPRSLSPLDEDAIFTSLEKTNRLIIIDEANPRCSIATDIAALVADKGFDLLDAPIKRITAPHTPVPFSPVLEDQY
LPTPDKIVSVTLELLGEPALN

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=330, Percent_Identity=39.3939393939394, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI291084858, Length=330, Percent_Identity=37.2727272727273, Blast_Score=224, Evalue=6e-59,
Organism=Homo sapiens, GI4557353, Length=322, Percent_Identity=38.1987577639752, Blast_Score=218, Evalue=9e-57,
Organism=Homo sapiens, GI34101272, Length=322, Percent_Identity=38.1987577639752, Blast_Score=218, Evalue=9e-57,
Organism=Caenorhabditis elegans, GI17538422, Length=333, Percent_Identity=40.5405405405405, Blast_Score=243, Evalue=9e-65,
Organism=Caenorhabditis elegans, GI17506935, Length=328, Percent_Identity=42.0731707317073, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6319698, Length=340, Percent_Identity=39.1176470588235, Blast_Score=233, Evalue=3e-62,
Organism=Drosophila melanogaster, GI21358145, Length=341, Percent_Identity=38.41642228739, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24650940, Length=341, Percent_Identity=38.41642228739, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI160714832, Length=329, Percent_Identity=41.0334346504559, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI160714828, Length=329, Percent_Identity=41.0334346504559, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24650943, Length=105, Percent_Identity=34.2857142857143, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24650945, Length=105, Percent_Identity=34.2857142857143, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACOB_BACSU (O34591)

Other databases:

- EMBL:   AF006075
- EMBL:   D78509
- EMBL:   AL009126
- PIR:   E69581
- RefSeq:   NP_388688.1
- ProteinModelPortal:   O34591
- SMR:   O34591
- EnsemblBacteria:   EBBACT00000001780
- GeneID:   939697
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08070
- NMPDR:   fig|224308.1.peg.807
- GenoList:   BSU08070
- GeneTree:   EBGT00050000000572
- HOGENOM:   HBG753264
- OMA:   NDFIGTC
- PhylomeDB:   O34591
- ProtClustDB:   CLSK873141
- BioCyc:   BSUB:BSU08070-MONOMER
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476
- Gene3D:   G3DSA:3.40.50.920
- SMART:   SM00861

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C; SSF52922 Transketo_C_like

EC number: 1.2.4.1

Molecular weight: Translated: 36845; Mature: 36714

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARVISMSDAINEAMKLAMRKDENVLLIGEDVAGGAAVDHLQDDEAWGGVLGVTKGLVQE
CCEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHH
FGRTRVLDTPISEAGYMGAAMAAASTGLRPIAELMFNDFIGTCFDQVINQGAKFRYMFGG
HCCCEEECCCCHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
KAQVPITVRTTYGAGFRAAAQHSQSLYGLFTSIPGLKTVVPSNPYDAKGLLLAAIEDNDP
CEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEEECCCC
VFFFEDKTSYNMKGEVPEDYYTIPLGKADIKREGNDVTLFAVGKQVNTALEAAAQLSERG
EEEEECCCCCCCCCCCCCCEEEECCCCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCC
IEAEVLDPRSLSPLDEDAIFTSLEKTNRLIIIDEANPRCSIATDIAALVADKGFDLLDAP
CCEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHH
IKRITAPHTPVPFSPVLEDQYLPTPDKIVSVTLELLGEPALN
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARVISMSDAINEAMKLAMRKDENVLLIGEDVAGGAAVDHLQDDEAWGGVLGVTKGLVQE
CEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHH
FGRTRVLDTPISEAGYMGAAMAAASTGLRPIAELMFNDFIGTCFDQVINQGAKFRYMFGG
HCCCEEECCCCHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
KAQVPITVRTTYGAGFRAAAQHSQSLYGLFTSIPGLKTVVPSNPYDAKGLLLAAIEDNDP
CEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEEECCCC
VFFFEDKTSYNMKGEVPEDYYTIPLGKADIKREGNDVTLFAVGKQVNTALEAAAQLSERG
EEEEECCCCCCCCCCCCCCEEEECCCCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCC
IEAEVLDPRSLSPLDEDAIFTSLEKTNRLIIIDEANPRCSIATDIAALVADKGFDLLDAP
CCEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHH
IKRITAPHTPVPFSPVLEDQYLPTPDKIVSVTLELLGEPALN
HHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10368162; 8969503; 9384377