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The map label for this gene is acoA

Identifier: 16077873

GI number: 16077873

Start: 879002

End: 880003

Strand: Direct

Name: acoA

Synonym: BSU08060

Alternate gene names: 16077873

Gene position: 879002-880003 (Clockwise)

Preceding gene: 16077872

Following gene: 16077874

Centisome position: 20.85

GC content: 47.6

Gene sequence:

>1002_bases
ATGAAATTGTTAAAACGAGAAGGCTTGTCATTAACTGAGGAAAAAGCGCTGTGGATGTACCAAAAGATGCTGGAGATCAG
GGGCTTTGAAGACAAAGTGCATGAACTGTTCGCCCAGGGAGTGCTTCCCGGATTCGTTCATTTATATGCCGGTGAGGAAG
CCGTGGCTGTAGGGGTGTGCGCTCATTTACATGATGGCGACAGCATTACAAGCACCCACAGGGGACATGGACATTGTATC
GCCAAAGGCTGTGACCTGGACGGCATGATGGCGGAAATTTTCGGGAAAGCGACCGGATTGTGCAAAGGCAAGGGCGGTTC
TATGCACATTGCGGATCTTGATAAAGGCATGTTAGGCGCAAATGGAATCGTCGGGGGCGGCTTTACGCTCGCATGCGGAT
CAGCGCTCACGGCTAAATATAAACAGACTAAAAATGTAAGCGTTTGCTTTTTCGGGGACGGGGCAAATAACCAAGGTACC
TTCCACGAAGGGCTGAATTTAGCGGCTGTATGGAACCTTCCTGTCGTATTTGTTGCTGAAAACAACGGCTATGGCGAAGC
TACCCCATTTGAGTACGCATCAGCCTGTGATTCAATCGCCGATCGGGCGGCTGCTTATAACATGCCGGGGGTTACAGTTG
ACGGCAAAGATATTTTAGCAGTTTACCAGGCAGCCGAGGAAGCGATAGAAAGAGCAAGAAACGGCGGCGGCCCGTCTTTG
ATTGAATGTATGACCTACAGAAACTACGGCCATTTCGAAGGAGATGCCCAAACCTATAAAACGAAGGATGAAAGAGTTGA
GCACCTTGAAGAAAAAGATGCCATTCAAGGTTTTAAAAACTACCTTTTAAAAGAAACAGATGCTAATAAGCTGTCAGACA
TTGAACAGCGTGTCAGCGAATCGATTGAAAAAGCCGTCTCGTTCAGCGAAGACAGCCCATATCCAAAAGATTCGGAGCTG
CTGACAGATGTGTATGTGTCATATGAAAAAGGAGGAATGTAA

Upstream 100 bases:

>100_bases
CTCCAAAGTGAAGATGAGAAGACTGATTTTACGGGCTCAAAAGACTGGCACACTTCTTGCATTTATAATGGTGAACCCTA
AATAGAAGGAGGCGCACAAA

Downstream 100 bases:

>100_bases
GAGATGGCGAGAGTCATAAGCATGTCAGACGCGATCAATGAAGCAATGAAGCTTGCGATGAGAAAAGACGAAAATGTGCT
TTTGATCGGTGAGGATGTCG

Product: acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKLLKREGLSLTEEKALWMYQKMLEIRGFEDKVHELFAQGVLPGFVHLYAGEEAVAVGVCAHLHDGDSITSTHRGHGHCI
AKGCDLDGMMAEIFGKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFTLACGSALTAKYKQTKNVSVCFFGDGANNQGT
FHEGLNLAAVWNLPVVFVAENNGYGEATPFEYASACDSIADRAAAYNMPGVTVDGKDILAVYQAAEEAIERARNGGGPSL
IECMTYRNYGHFEGDAQTYKTKDERVEHLEEKDAIQGFKNYLLKETDANKLSDIEQRVSESIEKAVSFSEDSPYPKDSEL
LTDVYVSYEKGGM

Sequences:

>Translated_333_residues
MKLLKREGLSLTEEKALWMYQKMLEIRGFEDKVHELFAQGVLPGFVHLYAGEEAVAVGVCAHLHDGDSITSTHRGHGHCI
AKGCDLDGMMAEIFGKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFTLACGSALTAKYKQTKNVSVCFFGDGANNQGT
FHEGLNLAAVWNLPVVFVAENNGYGEATPFEYASACDSIADRAAAYNMPGVTVDGKDILAVYQAAEEAIERARNGGGPSL
IECMTYRNYGHFEGDAQTYKTKDERVEHLEEKDAIQGFKNYLLKETDANKLSDIEQRVSESIEKAVSFSEDSPYPKDSEL
LTDVYVSYEKGGM
>Mature_333_residues
MKLLKREGLSLTEEKALWMYQKMLEIRGFEDKVHELFAQGVLPGFVHLYAGEEAVAVGVCAHLHDGDSITSTHRGHGHCI
AKGCDLDGMMAEIFGKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFTLACGSALTAKYKQTKNVSVCFFGDGANNQGT
FHEGLNLAAVWNLPVVFVAENNGYGEATPFEYASACDSIADRAAAYNMPGVTVDGKDILAVYQAAEEAIERARNGGGPSL
IECMTYRNYGHFEGDAQTYKTKDERVEHLEEKDAIQGFKNYLLKETDANKLSDIEQRVSESIEKAVSFSEDSPYPKDSEL
LTDVYVSYEKGGM

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=320, Percent_Identity=37.5, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI291084742, Length=320, Percent_Identity=37.8125, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI4505685, Length=320, Percent_Identity=37.8125, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI291084744, Length=327, Percent_Identity=37.0030581039755, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI291084757, Length=320, Percent_Identity=34.0625, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI11386135, Length=324, Percent_Identity=30.8641975308642, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI258645172, Length=324, Percent_Identity=30.8641975308642, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564172, Length=321, Percent_Identity=38.9408099688474, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17536047, Length=321, Percent_Identity=38.9408099688474, Blast_Score=213, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI86563357, Length=326, Percent_Identity=30.3680981595092, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI86563355, Length=326, Percent_Identity=30.3680981595092, Blast_Score=137, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6321026, Length=332, Percent_Identity=37.9518072289157, Blast_Score=199, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24639744, Length=320, Percent_Identity=38.4375, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI28571106, Length=320, Percent_Identity=38.4375, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24639740, Length=320, Percent_Identity=38.4375, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24639748, Length=323, Percent_Identity=38.0804953560372, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=38.562091503268, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI21355903, Length=316, Percent_Identity=26.8987341772152, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACOA_BACSU (O31404)

Other databases:

- EMBL:   D78509
- EMBL:   AF006075
- EMBL:   AL009126
- PIR:   D69581
- RefSeq:   NP_388687.1
- HSSP:   P21873
- ProteinModelPortal:   O31404
- SMR:   O31404
- EnsemblBacteria:   EBBACT00000003637
- GeneID:   936152
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08060
- NMPDR:   fig|224308.1.peg.806
- GenoList:   BSU08060
- GeneTree:   EBGT00050000001071
- HOGENOM:   HBG753263
- OMA:   NGYGEAT
- PhylomeDB:   O31404
- ProtClustDB:   CLSK873140
- BioCyc:   BSUB:BSU08060-MONOMER
- InterPro:   IPR001017

Pfam domain/function: PF00676 E1_dh

EC number: 1.2.4.1

Molecular weight: Translated: 36069; Mature: 36069

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLKREGLSLTEEKALWMYQKMLEIRGFEDKVHELFAQGVLPGFVHLYAGEEAVAVGVC
CCCCHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHEEECCCCCEEEEEE
AHLHDGDSITSTHRGHGHCIAKGCDLDGMMAEIFGKATGLCKGKGGSMHIADLDKGMLGA
EEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEEECCCCCEEEEECCCCCCCC
NGIVGGGFTLACGSALTAKYKQTKNVSVCFFGDGANNQGTFHEGLNLAAVWNLPVVFVAE
CCCCCCCHHHHCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCCEEEEECCCEEEEEC
NNGYGEATPFEYASACDSIADRAAAYNMPGVTVDGKDILAVYQAAEEAIERARNGGGPSL
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCHHH
IECMTYRNYGHFEGDAQTYKTKDERVEHLEEKDAIQGFKNYLLKETDANKLSDIEQRVSE
HHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SIEKAVSFSEDSPYPKDSELLTDVYVSYEKGGM
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLLKREGLSLTEEKALWMYQKMLEIRGFEDKVHELFAQGVLPGFVHLYAGEEAVAVGVC
CCCCHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHEEECCCCCEEEEEE
AHLHDGDSITSTHRGHGHCIAKGCDLDGMMAEIFGKATGLCKGKGGSMHIADLDKGMLGA
EEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEEECCCCCEEEEECCCCCCCC
NGIVGGGFTLACGSALTAKYKQTKNVSVCFFGDGANNQGTFHEGLNLAAVWNLPVVFVAE
CCCCCCCHHHHCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCCEEEEECCCEEEEEC
NNGYGEATPFEYASACDSIADRAAAYNMPGVTVDGKDILAVYQAAEEAIERARNGGGPSL
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCHHH
IECMTYRNYGHFEGDAQTYKTKDERVEHLEEKDAIQGFKNYLLKETDANKLSDIEQRVSE
HHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SIEKAVSFSEDSPYPKDSELLTDVYVSYEKGGM
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377