Click here to switch to the map view.

The map label for this gene is acoC

Identifier: 16077875

GI number: 16077875

Start: 881049

End: 882245

Strand: Direct

Name: acoC

Synonym: BSU08080

Alternate gene names: 16077875

Gene position: 881049-882245 (Clockwise)

Preceding gene: 16077874

Following gene: 16077876

Centisome position: 20.9

GC content: 49.46

Gene sequence:

>1197_bases
ATGGCGGTAAAAGTAGTGATGCCAAAATTGGGAATGGCCATGAAACAAGGGGAAGTATCGATATGGAATAAAAAAGTAGG
CGACCCGGTTGAAAAGGGAGAAAGCATTGCCAGCATTCAATCGGAGAAAATTGAAATGGAGATCGAAGCGCCTGAAAAAG
GAACGCTGATCGATATCAAAGTGAAAGAGGGAGAAGAGGTTCCGCCCGGCACAGCTATCTGCTATATCGGGGACGCCAAT
GAGTCGGTGCAGGAAGAGGCGGGGGCGCCTGTTGCTGAAGACAATATGCCGCAAGCCGTCCAGCCCGTCAAACAAGAAAA
CAAACCCGCAGCCTCCAAAAAAGATCGAATGAAAATATCTCCAGTCGCCAGGAAAATAGCAGAAAAAGCAGGATTAGACC
TAAAACAACTGAAAGGAACTGGACCAGGCGGACGAATCGTGAAGGATGACGTAACAAAGGCTCTTGCTGAACAGAAAAAA
GATCAAGCAAAGCCTGTTTCGGAGCAGAAAGCGCAGGAAATCCCGGTGACAGGCATGAGAAAGGTCATCGCTGCCCGAAT
GCAGGAAAGCCTGGCAAACAGCGCGCAGCTGACGATCACGATGAAAGCTGATATCACCAAGCTTGCCACTCTTCAAAAAC
AGCTTTCACCAACTGCGGAAGAGAGATACGGCACAAAACTGACGATCACTCATTTTGTCTCAAGAGCCGCCGTTCTCGCT
CTGCAAGCTCACCCTGTGCTGAACAGCTTTTATCAAAATGAGCGCATCATCACACATCCCCATGTGCACCTTGGTATGGC
TGTAGCCTTGGAAAATGGCTTAGTGGTGCCTGTCATCCGCCATGCTGAAAAGCTATCGCTGATTGAACTGGCTCAATCCA
TCTCAGAAAATGCCAAAAAAGCACGCGAGGGACGTGCGGGAAGCGAAGAACTGCAAGGATCTACTTTCTCCATTACAAAC
CTTGGCGCGTTTGGAGTTGAGCATTTCACACCGATACTAAATCCGCCGGAAACAGGCATTCTCGGCATCGGAGCAAGCTA
TGACACACCGGTGTATCAAGGGGAGGAGATCGTCAGAAGCACGATCCTGCCACTCAGCCTGACATTTGATCACAGAGCGT
GTGACGGCGCCCCTGCCGCTGCATTCCTGAAGGCGATGAAAACATATTTGGAAGAACCCGCAGCATTAATTTTATAG

Upstream 100 bases:

>100_bases
CCAGTGCTTGAAGATCAATATTTGCCGACACCAGATAAAATTGTCAGCGTCACGCTTGAATTGCTTGGCGAGCCGGCATT
GAATTAAAGGGGGGACACAA

Downstream 100 bases:

>100_bases
GAAAAGCAGGTGAAAACGACATGACATTAGCCATTATCGGCGGCGGACCTGCAGGCTATGCGGCTGCGGTTTCCGCGGCA
CAGCAGGGCAGAAACGTGCT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Acetoin dehydrogenase E2 component; Dihydrolipoamide acetyltransferase component of acetoin cleaving system

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAVKVVMPKLGMAMKQGEVSIWNKKVGDPVEKGESIASIQSEKIEMEIEAPEKGTLIDIKVKEGEEVPPGTAICYIGDAN
ESVQEEAGAPVAEDNMPQAVQPVKQENKPAASKKDRMKISPVARKIAEKAGLDLKQLKGTGPGGRIVKDDVTKALAEQKK
DQAKPVSEQKAQEIPVTGMRKVIAARMQESLANSAQLTITMKADITKLATLQKQLSPTAEERYGTKLTITHFVSRAAVLA
LQAHPVLNSFYQNERIITHPHVHLGMAVALENGLVVPVIRHAEKLSLIELAQSISENAKKAREGRAGSEELQGSTFSITN
LGAFGVEHFTPILNPPETGILGIGASYDTPVYQGEEIVRSTILPLSLTFDHRACDGAPAAAFLKAMKTYLEEPAALIL

Sequences:

>Translated_398_residues
MAVKVVMPKLGMAMKQGEVSIWNKKVGDPVEKGESIASIQSEKIEMEIEAPEKGTLIDIKVKEGEEVPPGTAICYIGDAN
ESVQEEAGAPVAEDNMPQAVQPVKQENKPAASKKDRMKISPVARKIAEKAGLDLKQLKGTGPGGRIVKDDVTKALAEQKK
DQAKPVSEQKAQEIPVTGMRKVIAARMQESLANSAQLTITMKADITKLATLQKQLSPTAEERYGTKLTITHFVSRAAVLA
LQAHPVLNSFYQNERIITHPHVHLGMAVALENGLVVPVIRHAEKLSLIELAQSISENAKKAREGRAGSEELQGSTFSITN
LGAFGVEHFTPILNPPETGILGIGASYDTPVYQGEEIVRSTILPLSLTFDHRACDGAPAAAFLKAMKTYLEEPAALIL
>Mature_397_residues
AVKVVMPKLGMAMKQGEVSIWNKKVGDPVEKGESIASIQSEKIEMEIEAPEKGTLIDIKVKEGEEVPPGTAICYIGDANE
SVQEEAGAPVAEDNMPQAVQPVKQENKPAASKKDRMKISPVARKIAEKAGLDLKQLKGTGPGGRIVKDDVTKALAEQKKD
QAKPVSEQKAQEIPVTGMRKVIAARMQESLANSAQLTITMKADITKLATLQKQLSPTAEERYGTKLTITHFVSRAAVLAL
QAHPVLNSFYQNERIITHPHVHLGMAVALENGLVVPVIRHAEKLSLIELAQSISENAKKAREGRAGSEELQGSTFSITNL
GAFGVEHFTPILNPPETGILGIGASYDTPVYQGEEIVRSTILPLSLTFDHRACDGAPAAAFLKAMKTYLEEPAALIL

Specific function: The 2-Oxoglutarate Dehydrogenase Complex Catalyzes The Overall Conversion Of 2-Oxoglutarate To Succinyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: 2- Oxoglutarate Dehydrogenase (E1), Dihydrolipoamide Succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI31711992, Length=437, Percent_Identity=30.8924485125858, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=27.7533039647577, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=27.7533039647577, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI110671329, Length=425, Percent_Identity=26.3529411764706, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI19923748, Length=222, Percent_Identity=32.8828828828829, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI260898739, Length=179, Percent_Identity=33.5195530726257, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1786946, Length=407, Percent_Identity=29.97542997543, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786305, Length=404, Percent_Identity=31.4356435643564, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17560088, Length=429, Percent_Identity=33.1002331002331, Blast_Score=206, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI25146366, Length=401, Percent_Identity=29.426433915212, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=419, Percent_Identity=25.7756563245823, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=27.8688524590164, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324258, Length=458, Percent_Identity=30.3493449781659, Blast_Score=179, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6320352, Length=407, Percent_Identity=29.7297297297297, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24582497, Length=291, Percent_Identity=31.2714776632302, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI20129315, Length=291, Percent_Identity=31.2714776632302, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=33.1858407079646, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI18859875, Length=425, Percent_Identity=25.8823529411765, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACOC_BACSU (O31550)

Other databases:

- EMBL:   D78509
- EMBL:   AL009126
- PIR:   F69581
- RefSeq:   NP_388689.1
- ProteinModelPortal:   O31550
- EnsemblBacteria:   EBBACT00000002928
- GeneID:   936147
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08080
- NMPDR:   fig|224308.1.peg.808
- GenoList:   BSU08080
- GeneTree:   EBGT00070000032116
- HOGENOM:   HBG630916
- OMA:   VESCIIT
- PhylomeDB:   O31550
- ProtClustDB:   PRK11856
- BioCyc:   BSUB:BSU08080-MONOMER
- BRENDA:   2.3.1.12
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- Gene3D:   G3DSA:4.10.320.10
- Gene3D:   G3DSA:3.30.559.10

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding; SSF47005 E3_bd; SSF51230 Hybrid_motif

EC number: =2.3.1.12

Molecular weight: Translated: 42886; Mature: 42755

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50968 BIOTINYL_LIPOYL; PS00189 LIPOYL

Important sites: ACT_SITE 371-371 ACT_SITE 375-375

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKVVMPKLGMAMKQGEVSIWNKKVGDPVEKGESIASIQSEKIEMEIEAPEKGTLIDIK
CCEEEECCHHCHHHHCCCEEECCCCCCCHHHHCCHHHHHHHCEEEEEEECCCCCCEEEEE
VKEGEEVPPGTAICYIGDANESVQEEAGAPVAEDNMPQAVQPVKQENKPAASKKDRMKIS
ECCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHCCCCHH
PVARKIAEKAGLDLKQLKGTGPGGRIVKDDVTKALAEQKKDQAKPVSEQKAQEIPVTGMR
HHHHHHHHHHCCCHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHH
KVIAARMQESLANSAQLTITMKADITKLATLQKQLSPTAEERYGTKLTITHFVSRAAVLA
HHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHCCCHHHHCCCEEEHHHHHHHHHHHH
LQAHPVLNSFYQNERIITHPHVHLGMAVALENGLVVPVIRHAEKLSLIELAQSISENAKK
HHHHHHHHHHHCCCCEEECCCHHHEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
AREGRAGSEELQGSTFSITNLGAFGVEHFTPILNPPETGILGIGASYDTPVYQGEEIVRS
HHHCCCCHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHH
TILPLSLTFDHRACDGAPAAAFLKAMKTYLEEPAALIL
HHCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHCC
>Mature Secondary Structure 
AVKVVMPKLGMAMKQGEVSIWNKKVGDPVEKGESIASIQSEKIEMEIEAPEKGTLIDIK
CEEEECCHHCHHHHCCCEEECCCCCCCHHHHCCHHHHHHHCEEEEEEECCCCCCEEEEE
VKEGEEVPPGTAICYIGDANESVQEEAGAPVAEDNMPQAVQPVKQENKPAASKKDRMKIS
ECCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHCCCCHH
PVARKIAEKAGLDLKQLKGTGPGGRIVKDDVTKALAEQKKDQAKPVSEQKAQEIPVTGMR
HHHHHHHHHHCCCHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHH
KVIAARMQESLANSAQLTITMKADITKLATLQKQLSPTAEERYGTKLTITHFVSRAAVLA
HHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHCCCHHHHCCCEEEHHHHHHHHHHHH
LQAHPVLNSFYQNERIITHPHVHLGMAVALENGLVVPVIRHAEKLSLIELAQSISENAKK
HHHHHHHHHHHCCCCEEECCCHHHEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
AREGRAGSEELQGSTFSITNLGAFGVEHFTPILNPPETGILGIGASYDTPVYQGEEIVRS
HHHCCCCHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHH
TILPLSLTFDHRACDGAPAAAFLKAMKTYLEEPAALIL
HHCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377