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The map label for this gene is nagBB

Identifier: 16077305

GI number: 16077305

Start: 256823

End: 257572

Strand: Reverse

Name: nagBB

Synonym: BSU02360

Alternate gene names: 16077305

Gene position: 257572-256823 (Counterclockwise)

Preceding gene: 16077309

Following gene: 16077304

Centisome position: 6.11

GC content: 44.93

Gene sequence:

>750_bases
ATGAAGATTCTGATCGCCGAGCACTACGAAGAACTCTGTAAGTTAAGCGCTGCCATTATTAAAGAACAAATACAAGCTAA
GAAAGATGCCGTTCTCGGCCTGGCAACAGGAAGCACACCGGTCGGCTTGTATAAGCAGTTGATTTCAGACTATCAGGCAG
GCGAAATTGATTTTTCAAAGGTTACTACATTTAATTTGGATGAATATGCTGGTCTCTCTCCATCTCATCCGCAAAGTTAT
AACCATTTTATGCATGAGCATCTTTTTCAGCATATCAATATGCAGCCTGATCATATTCATATCCCGCAAGGAGACAATCC
GCAGCTTGAGGCGGCATGCAAAGTATACGAGGATCTCATCCGCCAGGCGGGAGGCATTGATGTCCAAATTCTCGGCATCG
GCGCCAATGGCCATATCGGTTTTAATGAACCGGGATCTGATTTTGAAGACCGGACACGTGTTGTGAAGCTCTCGGAAAGC
ACGATACAGGCAAATGCCAGATTCTTCGGCGGCGATCCGGTGCTGGTTCCGAGACTCGCGATTTCAATGGGCATTAAAAC
CATCATGGAATTCAGCAAACATATTGTGCTTTTAGCAAGCGGCGAGGAAAAAGCAGACGCCATTCAAAAGATGGCTGAAG
GTCCTGTCACGACAGATGTACCAGCCTCCATTCTTCAAAAACACAATCATGTAACCGTTATCGCCGATTATAAAGCAGCT
CAAAAATTAAAGAGTGCATCATTCTCATAA

Upstream 100 bases:

>100_bases
TTGACAGTTTGATAAGGGCGGTGCTAAATTCGTAATGACAAGTATCATAAATTGGTCATAACAAATATGGTGCTTGTCTA
TCTCTTTGAGGGGGTGTCAC

Downstream 100 bases:

>100_bases
CACAATAAAAGGAGACTGTCATGTTTAAAAAGGCATTTCAAATTCTGCAGCAGCTTGGCCGCGCGTTGATGACTCCGGTT
GCCGTCCTGCCGGCAGCAGG

Product: glucosamine-6-phosphate isomerase

Products: NA

Alternate protein names: GlcN6P deaminase 2; GNPDA 2; Glucosamine-6-phosphate isomerase 2

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKILIAEHYEELCKLSAAIIKEQIQAKKDAVLGLATGSTPVGLYKQLISDYQAGEIDFSKVTTFNLDEYAGLSPSHPQSY
NHFMHEHLFQHINMQPDHIHIPQGDNPQLEAACKVYEDLIRQAGGIDVQILGIGANGHIGFNEPGSDFEDRTRVVKLSES
TIQANARFFGGDPVLVPRLAISMGIKTIMEFSKHIVLLASGEEKADAIQKMAEGPVTTDVPASILQKHNHVTVIADYKAA
QKLKSASFS

Sequences:

>Translated_249_residues
MKILIAEHYEELCKLSAAIIKEQIQAKKDAVLGLATGSTPVGLYKQLISDYQAGEIDFSKVTTFNLDEYAGLSPSHPQSY
NHFMHEHLFQHINMQPDHIHIPQGDNPQLEAACKVYEDLIRQAGGIDVQILGIGANGHIGFNEPGSDFEDRTRVVKLSES
TIQANARFFGGDPVLVPRLAISMGIKTIMEFSKHIVLLASGEEKADAIQKMAEGPVTTDVPASILQKHNHVTVIADYKAA
QKLKSASFS
>Mature_249_residues
MKILIAEHYEELCKLSAAIIKEQIQAKKDAVLGLATGSTPVGLYKQLISDYQAGEIDFSKVTTFNLDEYAGLSPSHPQSY
NHFMHEHLFQHINMQPDHIHIPQGDNPQLEAACKVYEDLIRQAGGIDVQILGIGANGHIGFNEPGSDFEDRTRVVKLSES
TIQANARFFGGDPVLVPRLAISMGIKTIMEFSKHIVLLASGEEKADAIQKMAEGPVTTDVPASILQKHNHVTVIADYKAA
QKLKSASFS

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=249, Percent_Identity=41.7670682730924, Blast_Score=205, Evalue=3e-53,
Organism=Homo sapiens, GI19923881, Length=249, Percent_Identity=38.9558232931727, Blast_Score=191, Evalue=6e-49,
Organism=Escherichia coli, GI1786893, Length=249, Percent_Identity=37.3493975903614, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1789530, Length=230, Percent_Identity=30, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI48994958, Length=236, Percent_Identity=28.8135593220339, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17554876, Length=248, Percent_Identity=43.9516129032258, Blast_Score=213, Evalue=9e-56,
Organism=Drosophila melanogaster, GI24581960, Length=249, Percent_Identity=41.3654618473896, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI19920764, Length=249, Percent_Identity=41.3654618473896, Blast_Score=204, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GAMA_BACSU (O31458)

Other databases:

- EMBL:   AB006424
- EMBL:   AL009126
- PIR:   E69750
- RefSeq:   NP_388118.1
- ProteinModelPortal:   O31458
- SMR:   O31458
- EnsemblBacteria:   EBBACT00000001347
- GeneID:   938425
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU02360
- NMPDR:   fig|224308.1.peg.237
- GenoList:   BSU02360
- GeneTree:   EBGT00050000002591
- HOGENOM:   HBG725991
- OMA:   IIAEHPI
- PhylomeDB:   O31458
- ProtClustDB:   CLSK2752305
- BioCyc:   BSUB:BSU02360-MONOMER
- BRENDA:   3.5.99.6
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 27289; Mature: 27289

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 67-67 ACT_SITE 136-136 ACT_SITE 138-138 ACT_SITE 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIAEHYEELCKLSAAIIKEQIQAKKDAVLGLATGSTPVGLYKQLISDYQAGEIDFSK
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCCCCCHHH
VTTFNLDEYAGLSPSHPQSYNHFMHEHLFQHINMQPDHIHIPQGDNPQLEAACKVYEDLI
EEEECCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHH
RQAGGIDVQILGIGANGHIGFNEPGSDFEDRTRVVKLSESTIQANARFFGGDPVLVPRLA
HHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHEEEECCHHHHCCCEEECCCCHHHHHHH
ISMGIKTIMEFSKHIVLLASGEEKADAIQKMAEGPVTTDVPASILQKHNHVTVIADYKAA
HHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEECCHHH
QKLKSASFS
HHHHHCCCC
>Mature Secondary Structure
MKILIAEHYEELCKLSAAIIKEQIQAKKDAVLGLATGSTPVGLYKQLISDYQAGEIDFSK
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCCCCCHHH
VTTFNLDEYAGLSPSHPQSYNHFMHEHLFQHINMQPDHIHIPQGDNPQLEAACKVYEDLI
EEEECCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHH
RQAGGIDVQILGIGANGHIGFNEPGSDFEDRTRVVKLSESTIQANARFFGGDPVLVPRLA
HHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHEEEECCHHHHCCCEEECCCCHHHHHHH
ISMGIKTIMEFSKHIVLLASGEEKADAIQKMAEGPVTTDVPASILQKHNHVTVIADYKAA
HHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEECCHHH
QKLKSASFS
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377