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The map label for this gene is nagP

Identifier: 16077304

GI number: 16077304

Start: 254907

End: 256802

Strand: Reverse

Name: nagP

Synonym: BSU02350

Alternate gene names: 16077304

Gene position: 256802-254907 (Counterclockwise)

Preceding gene: 16077305

Following gene: 16077303

Centisome position: 6.09

GC content: 48.52

Gene sequence:

>1896_bases
ATGTTTAAAAAGGCATTTCAAATTCTGCAGCAGCTTGGCCGCGCGTTGATGACTCCGGTTGCCGTCCTGCCGGCAGCAGG
TCTTTTGCTCCGTTTCGGAGACAAGGATTTACTGAACATCCCTATTATAAAGGATGCGGGCGGCGTTGTATTCGATAACC
TTCCGCTGATTTTCGCCGTGGGGGTCGCGATCGGATTAGCCGGCGGAGAAGGTGTAGCCGGACTTGCCGCTGTCATCGGA
TACTTAATTTTAACCGTCACCCTCGACAATATGGGAAAATTACTCGGCCTTCAGCCTCCGTATGAAGGAGCCGAGCACCT
GATTGATATGGGGGTTTTCGGAGGAATCATTATCGGGCTTCTCGCCGCCTATTTATATAAGCGATTTTCATCAATCGAGC
TGCATCCGGTCCTTGGATTTTTCTCTGGAAAACGCTTTGTCCCGATCATTACTTCCGTCAGTTCATTGGTGATCGGCGTC
ATTTTCTCATTCGTTTGGCCGCTGATCCAAAACGGAATTAACGCAGCCAGCAGTTTAATTGCCGATTCAACAGTCGGCCT
GTTCTTTTATGCAACGATCTATCGTTTGCTGATTCCTTTTGGCCTGCATCATATTTTTTATACGCCTTTCTATTTTATGA
TGGGAGAATACACAGACCCATCAACAGGCAACACCGTAACCGGCGACCTGACAAGGTTTTTCGCGGGAGACCCGACAGCC
GGGCGCTTTATGATGGGGGATTTCCCTTACATGATTTTCTGTCTGCCGGCCGTTGCTCTTGCCATTATCCATACCGCGCG
TCCGGAAAAGAAAAAAATGATCTCCGGCGTGATGATTTCTGCGGCGCTCACATCTATGCTGACCGGAATTACAGAACCGG
TTGAGTTTTCTTTCTTGTTCGTTGCGCCCGTGCTCTATTTGATTAACTCTATTCTTGCCGGCGTCATCTTTGTCGTTTGT
GATTTATTCCATGTCCGCCACGGCTATACGTTCTCAGGCGGAGGAATTGACTACGTCTTGAACTACGGACTGTCTACAAA
CGGCTGGGTTGTCATTCCTGTCGGGATCGTGTTTGCTTTCATTTATTATTATTTGTTCCGATTTGCGATTCTGAAATGGA
ATTTGAAAACGCCCGGCCGTGAGACAGATGAAGACGGTCAGAATGAGGAGAAAGCTCCAGTTGCAAAAGACCAGCTTGCC
TTTCACGTGCTGCAGGCCTTGGGCGGACAGCAAAATATCGCTAACCTGGATGCATGTATCACAAGGCTTCGTGTGACCGT
TCATCAGCCAAGCCAAGTCTGCAAGGATGAATTAAAACGGCTTGGTGCAGTAGGTGTGCTGGAGGTCAACAACAACTTCC
AGGCAATATTCGGAACCAAGTCCGATGCCTTAAAAGACGACATCAAAACAATCATGGCCGGAGGCGTCCCTGCCACCGCC
GCCGCGCTGGACACGGTAACTGACAAACCGCTGAAGCCCGATTCCGATGAGACCTTTATTTATCCGATTAAAGGAGAAAC
AGTCTCTTTAGGGGATGTGCCTGATCAGGTCTTTTCAGAAAAAATGATGGGCGAGGGATTTGCGATTATACCATCAGAAG
GAAAAGTCGTTGCACCTGCGGACGGCGAGATCGTCTCGATTTTTCCGACAAAACACGCCATCGGCTTTATGAGCGCCGGC
GGCACTGAAATCCTGATTCATGTCGGCATCGATACCGTCAAACTGAATGGGGAAGGCTTTGAAGCACATGTCACAAGCGG
ACAAGCCGTCAAACAAGGCGAACTGCTTCTCACCTTTGATCTCAATTACATCAAGCAGCATGCCGCTTCAGCCATAACAC
CGGTTATTTTCACAAATACCTCTGAAGAAGATCTAAAGCACATTCAAATGAAGTAA

Upstream 100 bases:

>100_bases
TTCTTCAAAAACACAATCATGTAACCGTTATCGCCGATTATAAAGCAGCTCAAAAATTAAAGAGTGCATCATTCTCATAA
CACAATAAAAGGAGACTGTC

Downstream 100 bases:

>100_bases
AAAAGTCCCCCCTGCTGCGGGGGACTTTTTCGCCAACATAATGAAAGCGCTGTCAAAATAAAATAACTTGAAAAAAGGTG
TTTGCATTTGAATCACGAAT

Product: phosphotransferase system (PTS) glucosamine-specific enzyme IICBA component

Products: NA

Alternate protein names: Glucosamine permease IIC component; PTS system glucosamine-specific EIIC component; Glucosamine-specific phosphotransferase enzyme IIB component; PTS system glucosamine-specific EIIB component; Glucosamine-specific phosphotransferase enzyme IIA component; PTS system glucosamine-specific EIIA component

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MFKKAFQILQQLGRALMTPVAVLPAAGLLLRFGDKDLLNIPIIKDAGGVVFDNLPLIFAVGVAIGLAGGEGVAGLAAVIG
YLILTVTLDNMGKLLGLQPPYEGAEHLIDMGVFGGIIIGLLAAYLYKRFSSIELHPVLGFFSGKRFVPIITSVSSLVIGV
IFSFVWPLIQNGINAASSLIADSTVGLFFYATIYRLLIPFGLHHIFYTPFYFMMGEYTDPSTGNTVTGDLTRFFAGDPTA
GRFMMGDFPYMIFCLPAVALAIIHTARPEKKKMISGVMISAALTSMLTGITEPVEFSFLFVAPVLYLINSILAGVIFVVC
DLFHVRHGYTFSGGGIDYVLNYGLSTNGWVVIPVGIVFAFIYYYLFRFAILKWNLKTPGRETDEDGQNEEKAPVAKDQLA
FHVLQALGGQQNIANLDACITRLRVTVHQPSQVCKDELKRLGAVGVLEVNNNFQAIFGTKSDALKDDIKTIMAGGVPATA
AALDTVTDKPLKPDSDETFIYPIKGETVSLGDVPDQVFSEKMMGEGFAIIPSEGKVVAPADGEIVSIFPTKHAIGFMSAG
GTEILIHVGIDTVKLNGEGFEAHVTSGQAVKQGELLLTFDLNYIKQHAASAITPVIFTNTSEEDLKHIQMK

Sequences:

>Translated_631_residues
MFKKAFQILQQLGRALMTPVAVLPAAGLLLRFGDKDLLNIPIIKDAGGVVFDNLPLIFAVGVAIGLAGGEGVAGLAAVIG
YLILTVTLDNMGKLLGLQPPYEGAEHLIDMGVFGGIIIGLLAAYLYKRFSSIELHPVLGFFSGKRFVPIITSVSSLVIGV
IFSFVWPLIQNGINAASSLIADSTVGLFFYATIYRLLIPFGLHHIFYTPFYFMMGEYTDPSTGNTVTGDLTRFFAGDPTA
GRFMMGDFPYMIFCLPAVALAIIHTARPEKKKMISGVMISAALTSMLTGITEPVEFSFLFVAPVLYLINSILAGVIFVVC
DLFHVRHGYTFSGGGIDYVLNYGLSTNGWVVIPVGIVFAFIYYYLFRFAILKWNLKTPGRETDEDGQNEEKAPVAKDQLA
FHVLQALGGQQNIANLDACITRLRVTVHQPSQVCKDELKRLGAVGVLEVNNNFQAIFGTKSDALKDDIKTIMAGGVPATA
AALDTVTDKPLKPDSDETFIYPIKGETVSLGDVPDQVFSEKMMGEGFAIIPSEGKVVAPADGEIVSIFPTKHAIGFMSAG
GTEILIHVGIDTVKLNGEGFEAHVTSGQAVKQGELLLTFDLNYIKQHAASAITPVIFTNTSEEDLKHIQMK
>Mature_631_residues
MFKKAFQILQQLGRALMTPVAVLPAAGLLLRFGDKDLLNIPIIKDAGGVVFDNLPLIFAVGVAIGLAGGEGVAGLAAVIG
YLILTVTLDNMGKLLGLQPPYEGAEHLIDMGVFGGIIIGLLAAYLYKRFSSIELHPVLGFFSGKRFVPIITSVSSLVIGV
IFSFVWPLIQNGINAASSLIADSTVGLFFYATIYRLLIPFGLHHIFYTPFYFMMGEYTDPSTGNTVTGDLTRFFAGDPTA
GRFMMGDFPYMIFCLPAVALAIIHTARPEKKKMISGVMISAALTSMLTGITEPVEFSFLFVAPVLYLINSILAGVIFVVC
DLFHVRHGYTFSGGGIDYVLNYGLSTNGWVVIPVGIVFAFIYYYLFRFAILKWNLKTPGRETDEDGQNEEKAPVAKDQLA
FHVLQALGGQQNIANLDACITRLRVTVHQPSQVCKDELKRLGAVGVLEVNNNFQAIFGTKSDALKDDIKTIMAGGVPATA
AALDTVTDKPLKPDSDETFIYPIKGETVSLGDVPDQVFSEKMMGEGFAIIPSEGKVVAPADGEIVSIFPTKHAIGFMSAG
GTEILIHVGIDTVKLNGEGFEAHVTSGQAVKQGELLLTFDLNYIKQHAASAITPVIFTNTSEEDLKHIQMK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain

Homologues:

Organism=Escherichia coli, GI1786894, Length=632, Percent_Identity=42.246835443038, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1787343, Length=476, Percent_Identity=48.109243697479, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1787908, Length=515, Percent_Identity=35.9223300970874, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1788757, Length=124, Percent_Identity=47.5806451612903, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1790159, Length=152, Percent_Identity=42.1052631578947, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTW3C_BACSU (P39816)

Other databases:

- EMBL:   AB006424
- EMBL:   AL009126
- EMBL:   U15147
- PIR:   D69750
- RefSeq:   NP_388117.1
- ProteinModelPortal:   P39816
- EnsemblBacteria:   EBBACT00000001029
- GeneID:   938418
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU02350
- NMPDR:   fig|224308.1.peg.236
- GenoList:   BSU02350
- GeneTree:   EBGT00050000000032
- HOGENOM:   HBG571563
- OMA:   DAIFTNL
- PhylomeDB:   P39816
- BioCyc:   BSUB:BSU02350-MONOMER
- BRENDA:   2.7.1.69
- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011299
- Gene3D:   G3DSA:3.30.1360.60
- TIGRFAMs:   TIGR00826
- TIGRFAMs:   TIGR00830
- TIGRFAMs:   TIGR02002

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC; SSF51261 Dup_hybrid_motif; SSF55604 PTS_EIIB

EC number: =2.7.1.69

Molecular weight: Translated: 68145; Mature: 68145

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS51093 PTS_EIIA_TYPE_1; PS00371 PTS_EIIA_TYPE_1_HIS; PS51098 PTS_EIIB_TYPE_1; PS01035 PTS_EIIB_TYPE_1_CYS; PS51103 PTS_EIIC_TYPE_1

Important sites: ACT_SITE 419-419 ACT_SITE 567-567

Signals:

None

Transmembrane regions:

HASH(0xbe24340)-; HASH(0xc408f70)-; HASH(0xc850380)-; HASH(0xc4d0f10)-; HASH(0xbbcea60)-; HASH(0xc5bdb64)-; HASH(0xc069b64)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKAFQILQQLGRALMTPVAVLPAAGLLLRFGDKDLLNIPIIKDAGGVVFDNLPLIFAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCEEECCCCEEECCCHHHHHH
GVAIGLAGGEGVAGLAAVIGYLILTVTLDNMGKLLGLQPPYEGAEHLIDMGVFGGIIIGL
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LAAYLYKRFSSIELHPVLGFFSGKRFVPIITSVSSLVIGVIFSFVWPLIQNGINAASSLI
HHHHHHHHHCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADSTVGLFFYATIYRLLIPFGLHHIFYTPFYFMMGEYTDPSTGNTVTGDLTRFFAGDPTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHCCCCCC
GRFMMGDFPYMIFCLPAVALAIIHTARPEKKKMISGVMISAALTSMLTGITEPVEFSFLF
CCEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VAPVLYLINSILAGVIFVVCDLFHVRHGYTFSGGGIDYVLNYGLSTNGWVVIPVGIVFAF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCCCCCEEEEEHHHHHHH
IYYYLFRFAILKWNLKTPGRETDEDGQNEEKAPVAKDQLAFHVLQALGGQQNIANLDACI
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
TRLRVTVHQPSQVCKDELKRLGAVGVLEVNNNFQAIFGTKSDALKDDIKTIMAGGVPATA
HHHHHEECCHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCHHH
AALDTVTDKPLKPDSDETFIYPIKGETVSLGDVPDQVFSEKMMGEGFAIIPSEGKVVAPA
HHHHHHCCCCCCCCCCCEEEEEECCCEEECCCCCHHHHHHHHHCCCEEEECCCCCEECCC
DGEIVSIFPTKHAIGFMSAGGTEILIHVGIDTVKLNGEGFEAHVTSGQAVKQGELLLTFD
CCCEEEEECCCHHHEEECCCCCEEEEEECCEEEEECCCCCEEEECCCCCEECCCEEEEEC
LNYIKQHAASAITPVIFTNTSEEDLKHIQMK
HHHHHHHHHHHCCCEEEECCCHHHHHHHCCH
>Mature Secondary Structure
MFKKAFQILQQLGRALMTPVAVLPAAGLLLRFGDKDLLNIPIIKDAGGVVFDNLPLIFAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCEEECCCCEEECCCHHHHHH
GVAIGLAGGEGVAGLAAVIGYLILTVTLDNMGKLLGLQPPYEGAEHLIDMGVFGGIIIGL
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LAAYLYKRFSSIELHPVLGFFSGKRFVPIITSVSSLVIGVIFSFVWPLIQNGINAASSLI
HHHHHHHHHCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADSTVGLFFYATIYRLLIPFGLHHIFYTPFYFMMGEYTDPSTGNTVTGDLTRFFAGDPTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHCCCCCC
GRFMMGDFPYMIFCLPAVALAIIHTARPEKKKMISGVMISAALTSMLTGITEPVEFSFLF
CCEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VAPVLYLINSILAGVIFVVCDLFHVRHGYTFSGGGIDYVLNYGLSTNGWVVIPVGIVFAF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCCCCCEEEEEHHHHHHH
IYYYLFRFAILKWNLKTPGRETDEDGQNEEKAPVAKDQLAFHVLQALGGQQNIANLDACI
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
TRLRVTVHQPSQVCKDELKRLGAVGVLEVNNNFQAIFGTKSDALKDDIKTIMAGGVPATA
HHHHHEECCHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCHHH
AALDTVTDKPLKPDSDETFIYPIKGETVSLGDVPDQVFSEKMMGEGFAIIPSEGKVVAPA
HHHHHHCCCCCCCCCCCEEEEEECCCEEECCCCCHHHHHHHHHCCCEEEECCCCCEECCC
DGEIVSIFPTKHAIGFMSAGGTEILIHVGIDTVKLNGEGFEAHVTSGQAVKQGELLLTFD
CCCEEEEECCCHHHEEECCCCCEEEEEECCEEEEECCCCCEEEECCCCCEECCCEEEEEC
LNYIKQHAASAITPVIFTNTSEEDLKHIQMK
HHHHHHHHHHHCCCEEEECCCHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 7751298