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The map label for this gene is ybfI

Identifier: 16077291

GI number: 16077291

Start: 242834

End: 243661

Strand: Reverse

Name: ybfI

Synonym: BSU02220

Alternate gene names: 16077291

Gene position: 243661-242834 (Counterclockwise)

Preceding gene: 16077303

Following gene: 16077290

Centisome position: 5.78

GC content: 43.84

Gene sequence:

>828_bases
ATGCAAAACGAAACCCGCACACTGCAGCTTGACCCACACCTTCATATAGAAGCCTACCGCTTCAAGGGAATTATGCAGAA
GTTCCCGAACCACTTTCACGATTATTATGTGATTGGATTTATTGAAAAAGGGCAACGGTATCTGGCCTGTCAGGATCAAG
AATATATCATCAATCCAGGTGATCTTCTCCTCTTTAACCCCCGTGACACGCATAGCTGTGAACAGATTGACGGCCGGACG
CTTGATTATCGGTGTATCAATGTCATGCCGGATATCATGGAGAAGGCGGTGAAAGAGATTACTGGCTCCGGGCATCTCCC
TTATTTCTCCCAGCATGTGTTATTCCGCCATGAGCTGACAGCCAGCCTGCAGGAATTGCATATTTTGATATCCGAGGAAA
AACAAGCTTTGCGTAAGGAGGAGCTGTTTCTTCATCTATTAGAGGAGCTGATCCGCCATTATTCAGATGTCACGTTCCTT
AGCAGCGTTCCAGAACCATCAGACGAAGTCAAAATGGTTTGTGAATTTTTAGAAGAGCATTATGCCGAAAATGTTACACT
CAATGATTTGAGTGAATTAACAGGCTGGAGCAAGTATCATTTATTGCGTTCATTTACAAAACAAAAAGGCATTACCCCTA
ATAGCTACATGGAAACAATCCGGATTAATCAAGCCAAAAAACTCCTTGAACAAGGGGTAAGGCCGATTGATGCGGCATTT
CAGACAGGATTCAGCGATCAGAGTCATATGACGAAGTTTTTTAAACGGCAGGTGGGGCTGACGCCGAAGCAATACATGAA
GATTTTTGAAAAGGAGCTTCACAGATGA

Upstream 100 bases:

>100_bases
GGTCTGCTTTCTCTATTCCTAAACAGCAACATCATACAAGAAATCCCCCAAAATCATGCTACACTTAATCCAAGACCACC
ACACCGACAGGAGGCATCCC

Downstream 100 bases:

>100_bases
ACATACAAAGAGAAACTTCGGGGCATATCGCAGCCATTGTGACCATTCTGATTTGGGGGACGACCTTTATTTCGACAAAA
GTTCTGCTGGCTGACTTCTC

Product: AraC/XylS family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MQNETRTLQLDPHLHIEAYRFKGIMQKFPNHFHDYYVIGFIEKGQRYLACQDQEYIINPGDLLLFNPRDTHSCEQIDGRT
LDYRCINVMPDIMEKAVKEITGSGHLPYFSQHVLFRHELTASLQELHILISEEKQALRKEELFLHLLEELIRHYSDVTFL
SSVPEPSDEVKMVCEFLEEHYAENVTLNDLSELTGWSKYHLLRSFTKQKGITPNSYMETIRINQAKKLLEQGVRPIDAAF
QTGFSDQSHMTKFFKRQVGLTPKQYMKIFEKELHR

Sequences:

>Translated_275_residues
MQNETRTLQLDPHLHIEAYRFKGIMQKFPNHFHDYYVIGFIEKGQRYLACQDQEYIINPGDLLLFNPRDTHSCEQIDGRT
LDYRCINVMPDIMEKAVKEITGSGHLPYFSQHVLFRHELTASLQELHILISEEKQALRKEELFLHLLEELIRHYSDVTFL
SSVPEPSDEVKMVCEFLEEHYAENVTLNDLSELTGWSKYHLLRSFTKQKGITPNSYMETIRINQAKKLLEQGVRPIDAAF
QTGFSDQSHMTKFFKRQVGLTPKQYMKIFEKELHR
>Mature_275_residues
MQNETRTLQLDPHLHIEAYRFKGIMQKFPNHFHDYYVIGFIEKGQRYLACQDQEYIINPGDLLLFNPRDTHSCEQIDGRT
LDYRCINVMPDIMEKAVKEITGSGHLPYFSQHVLFRHELTASLQELHILISEEKQALRKEELFLHLLEELIRHYSDVTFL
SSVPEPSDEVKMVCEFLEEHYAENVTLNDLSELTGWSKYHLLRSFTKQKGITPNSYMETIRINQAKKLLEQGVRPIDAAF
QTGFSDQSHMTKFFKRQVGLTPKQYMKIFEKELHR

Specific function: Positive Activator Of Genes Required For L-Rhamnose Utilization, Probably Of The Rhamnose Transporter Gene (Rhat). [C]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790339, Length=248, Percent_Identity=22.5806451612903, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786251, Length=253, Percent_Identity=24.901185770751, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YBFI_BACSU (O31449)

Other databases:

- EMBL:   AB006424
- EMBL:   AL009126
- PIR:   D69749
- RefSeq:   NP_388104.1
- ProteinModelPortal:   O31449
- EnsemblBacteria:   EBBACT00000001574
- GeneID:   938437
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU02220
- NMPDR:   fig|224308.1.peg.223
- GenoList:   BSU02220
- GeneTree:   EBGT00070000032305
- HOGENOM:   HBG345635
- OMA:   RYRMIYP
- PhylomeDB:   O31449
- ProtClustDB:   CLSK886613
- BioCyc:   BSUB:BSU02220-MONOMER
- GO:   GO:0005622
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018060
- Gene3D:   G3DSA:1.10.10.60
- SMART:   SM00342

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC; SSF51215 AraC_binding; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 32440; Mature: 32440

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00041 HTH_ARAC_FAMILY_1; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNETRTLQLDPHLHIEAYRFKGIMQKFPNHFHDYYVIGFIEKGQRYLACQDQEYIINPG
CCCCCCEEEECCCEEEHHHHHHHHHHHCCHHHCCEEEEEEEECCCEEEEECCCCEEECCC
DLLLFNPRDTHSCEQIDGRTLDYRCINVMPDIMEKAVKEITGSGHLPYFSQHVLFRHELT
CEEEECCCCCCCHHHHCCCEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ASLQELHILISEEKQALRKEELFLHLLEELIRHYSDVTFLSSVPEPSDEVKMVCEFLEEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHH
YAENVTLNDLSELTGWSKYHLLRSFTKQKGITPNSYMETIRINQAKKLLEQGVRPIDAAF
HHCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
QTGFSDQSHMTKFFKRQVGLTPKQYMKIFEKELHR
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQNETRTLQLDPHLHIEAYRFKGIMQKFPNHFHDYYVIGFIEKGQRYLACQDQEYIINPG
CCCCCCEEEECCCEEEHHHHHHHHHHHCCHHHCCEEEEEEEECCCEEEEECCCCEEECCC
DLLLFNPRDTHSCEQIDGRTLDYRCINVMPDIMEKAVKEITGSGHLPYFSQHVLFRHELT
CEEEECCCCCCCHHHHCCCEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ASLQELHILISEEKQALRKEELFLHLLEELIRHYSDVTFLSSVPEPSDEVKMVCEFLEEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHH
YAENVTLNDLSELTGWSKYHLLRSFTKQKGITPNSYMETIRINQAKKLLEQGVRPIDAAF
HHCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
QTGFSDQSHMTKFFKRQVGLTPKQYMKIFEKELHR
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377