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The map label for this gene is gltP

Identifier: 16077303

GI number: 16077303

Start: 253518

End: 254762

Strand: Reverse

Name: gltP

Synonym: BSU02340

Alternate gene names: 16077303

Gene position: 254762-253518 (Counterclockwise)

Preceding gene: 16077304

Following gene: 16077291

Centisome position: 6.04

GC content: 45.62

Gene sequence:

>1245_bases
TTGAAAAAATTAATCGCGTTTCAAATTTTAATCGCTTTGGCTGTCGGCGCGGTGATCGGCCACTTTTTTCCTGATTTCGG
GATGGCACTGAGGCCTGTCGGAGATGGATTTATCCGCCTGATTAAAATGATTGTTGTGCCGATTGTATTTTCTACCATTG
TCATTGGAGCCGCAGGAAGCGGAAGCATGAAAAAAATGGGCAGTCTCGGCATCAAGACGATCATTTGGTTCGAAGTGATT
ACAACGTTGGTATTAGGGCTTGGACTTTTATTAGCGAATGTCTTAAAACCAGGTGTCGGGCTTGACCTCTCCCATTTGGC
AAAAAAAGATATTCATGAACTTTCCGGCTACACAGACAAAGTTGTTGATTTCAAGCAAATGATCCTGGATATTATCCCTA
CAAACATTATTGATGTCATGGCCAGAAATGATTTGCTTGCCGTTATTTTCTTCGCCATTTTATTTGGTGTCGCCGCAGCT
GGTATCGGCAAAGCTTCTGAACCAGTAATGAAGTTTTTTGAATCTACAGCTCAGATTATGTTCAAGCTCACGCAAATCGT
CATGGTCACTGCTCCTATCGGCGTGTTAGCCCTGATGGCTGCTTCTGTAGGCCAATATGGCATTGAACTCCTCCTCCCTA
TGTTTAAGCTGGTTGGCACCGTATTCCTTGGCCTGTTCCTGATCCTCTTTGTCCTCTTTCCGCTTGTCGGTCTCATCTTT
CAGATTAAGTATTTCGAAGTATTGAAAATGATATGGGATCTGTTTTTAATTGCATTTTCCACCACAAGCACTGAAACCAT
TCTCCCTCAGCTGATGGATAGAATGGAGAAATACGGCTGTCCCAAACGAGTGGTATCATTTGTCGTTCCCTCCGGCTTGT
CATTGAACTGTGACGGCTCCAGCTTATATTTATCGGTTTCTTGTATCTTTCTCGCACAGGCCTTTCAAGTAGACATGACA
TTGTCCCAGCAGCTGCTCATGATGCTTGTGCTTGTGATGACCAGTAAAGGCATTGCAGCTGTACCCTCAGGATCGCTTGT
AGTTCTTCTGGCGACTGCCAATGCTGTAGGACTGCCGGCTGAAGGCGTTGCCATTATCGCGGGTGTTGACCGCGTCATGG
ACATGGCGAGAACAGGCGTGAACGTACCGGGACATGCGATCGCCTGTATCGTGGTATCAAAATGGGAAAAGGCCTTCCGC
CAAAAAGAATGGGTTTCCGCAAACAGCCAAACTGAAAGCATATAG

Upstream 100 bases:

>100_bases
AAGCGCTGTCAAAATAAAATAACTTGAAAAAAGGTGTTTGCATTTGAATCACGAATGATATGAATATGCCTACATTCTAT
CGTTCTAAAGGGGGATTCAT

Downstream 100 bases:

>100_bases
AAAAAAAGAACACCTCAATACGAGGTGTTCTTTTTTTATTCAGCAGCAGCGTGATCTTCTGTTTCAATCGCTGCCAGTCT
TGCGTTTACTTCTTCTTCTG

Product: proton/glutamate symport protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKKLIAFQILIALAVGAVIGHFFPDFGMALRPVGDGFIRLIKMIVVPIVFSTIVIGAAGSGSMKKMGSLGIKTIIWFEVI
TTLVLGLGLLLANVLKPGVGLDLSHLAKKDIHELSGYTDKVVDFKQMILDIIPTNIIDVMARNDLLAVIFFAILFGVAAA
GIGKASEPVMKFFESTAQIMFKLTQIVMVTAPIGVLALMAASVGQYGIELLLPMFKLVGTVFLGLFLILFVLFPLVGLIF
QIKYFEVLKMIWDLFLIAFSTTSTETILPQLMDRMEKYGCPKRVVSFVVPSGLSLNCDGSSLYLSVSCIFLAQAFQVDMT
LSQQLLMMLVLVMTSKGIAAVPSGSLVVLLATANAVGLPAEGVAIIAGVDRVMDMARTGVNVPGHAIACIVVSKWEKAFR
QKEWVSANSQTESI

Sequences:

>Translated_414_residues
MKKLIAFQILIALAVGAVIGHFFPDFGMALRPVGDGFIRLIKMIVVPIVFSTIVIGAAGSGSMKKMGSLGIKTIIWFEVI
TTLVLGLGLLLANVLKPGVGLDLSHLAKKDIHELSGYTDKVVDFKQMILDIIPTNIIDVMARNDLLAVIFFAILFGVAAA
GIGKASEPVMKFFESTAQIMFKLTQIVMVTAPIGVLALMAASVGQYGIELLLPMFKLVGTVFLGLFLILFVLFPLVGLIF
QIKYFEVLKMIWDLFLIAFSTTSTETILPQLMDRMEKYGCPKRVVSFVVPSGLSLNCDGSSLYLSVSCIFLAQAFQVDMT
LSQQLLMMLVLVMTSKGIAAVPSGSLVVLLATANAVGLPAEGVAIIAGVDRVMDMARTGVNVPGHAIACIVVSKWEKAFR
QKEWVSANSQTESI
>Mature_414_residues
MKKLIAFQILIALAVGAVIGHFFPDFGMALRPVGDGFIRLIKMIVVPIVFSTIVIGAAGSGSMKKMGSLGIKTIIWFEVI
TTLVLGLGLLLANVLKPGVGLDLSHLAKKDIHELSGYTDKVVDFKQMILDIIPTNIIDVMARNDLLAVIFFAILFGVAAA
GIGKASEPVMKFFESTAQIMFKLTQIVMVTAPIGVLALMAASVGQYGIELLLPMFKLVGTVFLGLFLILFVLFPLVGLIF
QIKYFEVLKMIWDLFLIAFSTTSTETILPQLMDRMEKYGCPKRVVSFVVPSGLSLNCDGSSLYLSVSCIFLAQAFQVDMT
LSQQLLMMLVLVMTSKGIAAVPSGSLVVLLATANAVGLPAEGVAIIAGVDRVMDMARTGVNVPGHAIACIVVSKWEKAFR
QKEWVSANSQTESI

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI21314632, Length=420, Percent_Identity=26.6666666666667, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI66773030, Length=427, Percent_Identity=25.2927400468384, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI40254478, Length=432, Percent_Identity=25.6944444444444, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI4827012, Length=275, Percent_Identity=28.7272727272727, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI223468564, Length=277, Percent_Identity=27.0758122743682, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI223468566, Length=277, Percent_Identity=27.0758122743682, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI169790839, Length=284, Percent_Identity=26.7605633802817, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI5032093, Length=406, Percent_Identity=24.8768472906404, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI194239697, Length=453, Percent_Identity=23.1788079470199, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI301601644, Length=175, Percent_Identity=29.1428571428571, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI262359914, Length=193, Percent_Identity=27.4611398963731, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1790514, Length=412, Percent_Identity=44.1747572815534, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1789947, Length=404, Percent_Identity=37.3762376237624, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1788024, Length=390, Percent_Identity=27.6923076923077, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71996953, Length=444, Percent_Identity=26.5765765765766, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17537407, Length=394, Percent_Identity=26.9035532994924, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71983106, Length=434, Percent_Identity=25.5760368663594, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71983099, Length=434, Percent_Identity=25.5760368663594, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI193206505, Length=444, Percent_Identity=26.5765765765766, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI193206654, Length=405, Percent_Identity=25.1851851851852, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17541374, Length=407, Percent_Identity=24.3243243243243, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24583025, Length=401, Percent_Identity=24.9376558603491, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137668, Length=401, Percent_Identity=24.9376558603491, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24583023, Length=401, Percent_Identity=24.9376558603491, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281360481, Length=446, Percent_Identity=25.3363228699552, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281360483, Length=447, Percent_Identity=25.2796420581655, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI17137666, Length=447, Percent_Identity=25.2796420581655, Blast_Score=92, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLTP_BACSU (P39817)

Other databases:

- EMBL:   U15147
- EMBL:   AB006424
- EMBL:   AL009126
- PIR:   B57142
- RefSeq:   NP_388116.1
- ProteinModelPortal:   P39817
- SMR:   P39817
- EnsemblBacteria:   EBBACT00000003806
- GeneID:   938421
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU02340
- NMPDR:   fig|224308.1.peg.235
- GenoList:   BSU02340
- GeneTree:   EBGT00070000031770
- HOGENOM:   HBG739804
- OMA:   FSTIVIG
- PhylomeDB:   P39817
- ProtClustDB:   CLSK886621
- BioCyc:   BSUB:BSU02340-MONOMER
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 44616; Mature: 44616

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc195428)-; HASH(0xc569c2c)-; HASH(0xbcbcb84)-; HASH(0xbd98660)-; HASH(0x40a94e48)-; HASH(0xc3306ac)-; HASH(0xb018774)-; HASH(0x40a793b0)-; HASH(0xbdfd328)-; HASH(0xc6c8428)-; HASH(0xbcabca8)-; HASH(0xb03a60c)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
>Mature Secondary Structure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CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751298; 9384377