Click here to switch to the map view.

The map label for this gene is bioD

Identifier: 15618950

GI number: 15618950

Start: 1195931

End: 1196590

Strand: Reverse

Name: bioD

Synonym: CPn1042

Alternate gene names: 15618950

Gene position: 1196590-1195931 (Counterclockwise)

Preceding gene: 15618951

Following gene: 15618949

Centisome position: 97.27

GC content: 42.42

Gene sequence:

>660_bases
ATGCAACGTATCATCATTGTAGGAATCGACACTGGCGTAGGAAAAACCATTGTCAGTGCTATCCTTGCTAGAGCACTTAA
CGCAGAATACTGGAAACCTATACAAGCAGGGAATCTAGAAAATTCAGATAGCAATATTGTTCATGAGCTATCGGGAGCCT
ACTGTCATCCCGAAGCTTATCGATTGCATAAGCCCTTGTCTCCACACAAGGCAGCGCAAATCGATAATGTAAGTATCGAA
GAGAGTCATATTTGTGCGCCAAAAACAACTTCGAATCTGATTATTGAGACTTCAGGAGGATTTTTATCCCCCTGCACATC
AAAAAGACTTCAGGGAGATGTGTTTTCTTCTTGGTCATGTTCTTGGATTTTAGTGAGCCAAGCATATCTCGGAAGTATCA
ATCACACCTGTTTAACGGTAGAAGCAATGCGCTCACGAAACCTCAATATCTTAGGTATGGTGGTAAATGGGTATCCAGAG
GACGAAGAGCACTGGCTAACTCAAGAAATCAAGCTTCCTATAATCGGGACTCTTGCCAAGGAAAAAGAAATCACAAAGAC
AATCATAAGCTGTTATGCCGAACAATGGAAGGAAGTATGGACAAGCAATCATCAGGGAATTCAGGGTGTATCTGGCACCC
CTTCACTCAATCTGCATTAG

Upstream 100 bases:

>100_bases
CAGTAAAACAAAGAGAAGAACTGTTACGTATTTGCCTTCATGCCTTCAATACAAAAAATGAAATAGATCACCTTTTACAT
ACTTTAGAGCAAATTTTTTT

Downstream 100 bases:

>100_bases
ATTCTACACCCATAAAGATTGTAAGGGGAGAAGGTGCTTACCTCTATGCGGAATCAGGAACAAGATATCTTGATGCGATA
TCTTCATGGTGGTGCAACCT

Product: dethiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MQRIIIVGIDTGVGKTIVSAILARALNAEYWKPIQAGNLENSDSNIVHELSGAYCHPEAYRLHKPLSPHKAAQIDNVSIE
ESHICAPKTTSNLIIETSGGFLSPCTSKRLQGDVFSSWSCSWILVSQAYLGSINHTCLTVEAMRSRNLNILGMVVNGYPE
DEEHWLTQEIKLPIIGTLAKEKEITKTIISCYAEQWKEVWTSNHQGIQGVSGTPSLNLH

Sequences:

>Translated_219_residues
MQRIIIVGIDTGVGKTIVSAILARALNAEYWKPIQAGNLENSDSNIVHELSGAYCHPEAYRLHKPLSPHKAAQIDNVSIE
ESHICAPKTTSNLIIETSGGFLSPCTSKRLQGDVFSSWSCSWILVSQAYLGSINHTCLTVEAMRSRNLNILGMVVNGYPE
DEEHWLTQEIKLPIIGTLAKEKEITKTIISCYAEQWKEVWTSNHQGIQGVSGTPSLNLH
>Mature_219_residues
MQRIIIVGIDTGVGKTIVSAILARALNAEYWKPIQAGNLENSDSNIVHELSGAYCHPEAYRLHKPLSPHKAAQIDNVSIE
ESHICAPKTTSNLIIETSGGFLSPCTSKRLQGDVFSSWSCSWILVSQAYLGSINHTCLTVEAMRSRNLNILGMVVNGYPE
DEEHWLTQEIKLPIIGTLAKEKEITKTIISCYAEQWKEVWTSNHQGIQGVSGTPSLNLH

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=202, Percent_Identity=29.2079207920792, Blast_Score=63, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324385, Length=178, Percent_Identity=30.3370786516854, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_CHLPN (Q9Z6L7)

Other databases:

- EMBL:   AE001363
- EMBL:   AE002161
- EMBL:   BA000008
- EMBL:   AE009440
- PIR:   F72004
- PIR:   G86620
- RefSeq:   NP_225236.1
- RefSeq:   NP_301097.1
- RefSeq:   NP_445349.1
- RefSeq:   NP_877354.1
- ProteinModelPortal:   Q9Z6L7
- SMR:   Q9Z6L7
- GeneID:   1467761
- GeneID:   895031
- GeneID:   919814
- GeneID:   962749
- GenomeReviews:   AE001363_GR
- GenomeReviews:   AE002161_GR
- GenomeReviews:   AE009440_GR
- GenomeReviews:   BA000008_GR
- KEGG:   cpa:CP0810
- KEGG:   cpn:CPn1042
- KEGG:   cpt:CpB1082
- TIGR:   CP_0810
- HOGENOM:   HBG650065
- OMA:   WKPIQSG
- PhylomeDB:   Q9Z6L7
- ProtClustDB:   CLSK399497
- BioCyc:   CPNE115711:CP_0810-MONOMER
- BioCyc:   CPNE115713:CPN1042-MONOMER
- BioCyc:   CPNE138677:CPJ1042-MONOMER
- BioCyc:   CPNE182082:CPB1082-MONOMER
- BRENDA:   6.3.3.3
- HAMAP:   MF_00336
- InterPro:   IPR004472
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: NA

EC number: =6.3.3.3

Molecular weight: Translated: 24165; Mature: 24165

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRIIIVGIDTGVGKTIVSAILARALNAEYWKPIQAGNLENSDSNIVHELSGAYCHPEAY
CCEEEEEEECCCCCHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHCCCEECCHHH
RLHKPLSPHKAAQIDNVSIEESHICAPKTTSNLIIETSGGFLSPCTSKRLQGDVFSSWSC
HHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCC
SWILVSQAYLGSINHTCLTVEAMRSRNLNILGMVVNGYPEDEEHWLTQEIKLPIIGTLAK
EEEEEEHHHHCCCCCCEEEEEHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEHHHH
EKEITKTIISCYAEQWKEVWTSNHQGIQGVSGTPSLNLH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQRIIIVGIDTGVGKTIVSAILARALNAEYWKPIQAGNLENSDSNIVHELSGAYCHPEAY
CCEEEEEEECCCCCHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHCCCEECCHHH
RLHKPLSPHKAAQIDNVSIEESHICAPKTTSNLIIETSGGFLSPCTSKRLQGDVFSSWSC
HHCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCC
SWILVSQAYLGSINHTCLTVEAMRSRNLNILGMVVNGYPEDEEHWLTQEIKLPIIGTLAK
EEEEEEHHHHCCCCCCEEEEEHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEHHHH
EKEITKTIISCYAEQWKEVWTSNHQGIQGVSGTPSLNLH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362