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The map label for this gene is emrB [H]

Identifier: 15606344

GI number: 15606344

Start: 735424

End: 736959

Strand: Direct

Name: emrB [H]

Synonym: aq_1062

Alternate gene names: 15606344

Gene position: 735424-736959 (Clockwise)

Preceding gene: 15606343

Following gene: 15606346

Centisome position: 47.41

GC content: 44.66

Gene sequence:

>1536_bases
ATGAGTGAGTTTTACGAGAAAATAAGCCCTTCCCAGAGGATTTTTCTCACCGTTTCCCTTATGGTCGGAGTCTTTATGGC
AATCCTTGACACCACTATAGTTGACATAATAGTTCCGAGACTCACAGGACCCCTTTCTACGGACCTTTACGGCGTTCAGT
GGGTAATAACGTCCTACATGACCGCAGCGGCAACGGGAATACTCCTCGTTGAGTGGCTTGAATCCAGAATAGGTTTAAAA
AAAGTTTTCCTGATTGGGCTTTTTCTCTTCACAACTTCTTCCTTCTTTGCGGGAAGGGCAAACTCCCTTGAGTGGATGAT
AACGGCGAGAACCTTTCAAGGTTTCGGGGAGTCTTTGATAGTGGTGAGTGCGGAAGCGATGCTCTTTTCCGCTTACCCGC
CCTACAAGAGAGGACTTGCGATGGGCATTTACGGACTTGGAGTGAGTTTTGCCCCGGCTCTCGGACCCACACTCGGAGGA
TGGATTACCGAACTCATAGAGTGGAGGTGGGTATTTTACATAAACATACCCATAGGGATAATAAACTTCGTTTCCGCTCT
TTTCTTCCTTCCCGAACACAAACCAAAACACTCCGTTTCCCTGAACTTCATCTCTTACTTTTTCATCTCCGTTGCCACCG
TTTCCCTTCTTATAGTCCTTTCCAAGGGGCAGCAACACGGCTGGTTTTCCTCCGACTTCATAGTTAAGATGTCTTTCCTG
AGCTTATTTTCCTTCCTCTTTTTCCTGCTTTCTGAGATGCTATCCAGAAACAAACTCGTGGACCCTGAAATATTCAAGAT
AAAGGAGTTCACGATAGCCTTTCTCGTTTACTGCCTGCTCCTTGGTTTTTCCATGTATCAGGTCTTTTACTTACTTCCCC
TCTTTTACGAGAGACTGAAAGGACTTCCCACCTTTGAGGCGGGACTTCACATTTTACCCTTAGCTCTGGCGGTTGGGATT
TCCTCACCGATTGCGGGAGTAATAAGCGACAAAGTTTCCGAAAGGCTTCCCCTCTTTATAGCAGTTGTTCTTTACCTTTA
CGCTTCCTTCTTCATACTTCCGAAGTTCAATTACTACACACCTTCCTGGGAATCTGCTTTCAAGTTGATAATACTCGGCA
TAGGTATGGGATTTTTCTTCGCTCCCATTACGAACCTCGCTCTCAAAAAACTCGGTGATAAGACCACTCTTGGAGTTAGC
CTTATGCACTATATAAGGTTCGTGGGAGGTTCTTTCGGAACTGCCCTTGCCACAAACGACATCCAGAGGTATATGTGGGA
AAACTTCCAGAGGAGCACGGAGATACAGAACGAAAGAATACTCTCCGAGTTCTTAGAAAAAATTTCGTGGCTCTTCTGGA
TAGGAGAAAAGAAAGTTAAAGCTCTTTTTTATCAGGTTGAACAACTTCACGCCTTTTCGGACAGCTTTATGAGAACCTTT
TTCGTTGCGGGACTCTGGGGACTTCTTGGAAGTTTACCTATCTTTTACCTTTTCTTTGAACTTCTATACGAAAAGTTTAT
AAAGAAGAGGGCTTAA

Upstream 100 bases:

>100_bases
AAGTTGTTCAGAGGATACCCGTAAAGATAAAGATTACGAAAGGGGATTTAAGCCTTCTGAGAGTGGGAATGGGCGGTGAG
GTTGAGATAAGGAGAACGAG

Downstream 100 bases:

>100_bases
AGCCCCCTTTCGGCGAGGTATATAACGAGGTCCCTGAGTCTGCAGGAGTAACCCCACTCGTTGTCATACCACGCGGCAAT
GTGGACAAGGTTGTCTATCA

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSEFYEKISPSQRIFLTVSLMVGVFMAILDTTIVDIIVPRLTGPLSTDLYGVQWVITSYMTAAATGILLVEWLESRIGLK
KVFLIGLFLFTTSSFFAGRANSLEWMITARTFQGFGESLIVVSAEAMLFSAYPPYKRGLAMGIYGLGVSFAPALGPTLGG
WITELIEWRWVFYINIPIGIINFVSALFFLPEHKPKHSVSLNFISYFFISVATVSLLIVLSKGQQHGWFSSDFIVKMSFL
SLFSFLFFLLSEMLSRNKLVDPEIFKIKEFTIAFLVYCLLLGFSMYQVFYLLPLFYERLKGLPTFEAGLHILPLALAVGI
SSPIAGVISDKVSERLPLFIAVVLYLYASFFILPKFNYYTPSWESAFKLIILGIGMGFFFAPITNLALKKLGDKTTLGVS
LMHYIRFVGGSFGTALATNDIQRYMWENFQRSTEIQNERILSEFLEKISWLFWIGEKKVKALFYQVEQLHAFSDSFMRTF
FVAGLWGLLGSLPIFYLFFELLYEKFIKKRA

Sequences:

>Translated_511_residues
MSEFYEKISPSQRIFLTVSLMVGVFMAILDTTIVDIIVPRLTGPLSTDLYGVQWVITSYMTAAATGILLVEWLESRIGLK
KVFLIGLFLFTTSSFFAGRANSLEWMITARTFQGFGESLIVVSAEAMLFSAYPPYKRGLAMGIYGLGVSFAPALGPTLGG
WITELIEWRWVFYINIPIGIINFVSALFFLPEHKPKHSVSLNFISYFFISVATVSLLIVLSKGQQHGWFSSDFIVKMSFL
SLFSFLFFLLSEMLSRNKLVDPEIFKIKEFTIAFLVYCLLLGFSMYQVFYLLPLFYERLKGLPTFEAGLHILPLALAVGI
SSPIAGVISDKVSERLPLFIAVVLYLYASFFILPKFNYYTPSWESAFKLIILGIGMGFFFAPITNLALKKLGDKTTLGVS
LMHYIRFVGGSFGTALATNDIQRYMWENFQRSTEIQNERILSEFLEKISWLFWIGEKKVKALFYQVEQLHAFSDSFMRTF
FVAGLWGLLGSLPIFYLFFELLYEKFIKKRA
>Mature_510_residues
SEFYEKISPSQRIFLTVSLMVGVFMAILDTTIVDIIVPRLTGPLSTDLYGVQWVITSYMTAAATGILLVEWLESRIGLKK
VFLIGLFLFTTSSFFAGRANSLEWMITARTFQGFGESLIVVSAEAMLFSAYPPYKRGLAMGIYGLGVSFAPALGPTLGGW
ITELIEWRWVFYINIPIGIINFVSALFFLPEHKPKHSVSLNFISYFFISVATVSLLIVLSKGQQHGWFSSDFIVKMSFLS
LFSFLFFLLSEMLSRNKLVDPEIFKIKEFTIAFLVYCLLLGFSMYQVFYLLPLFYERLKGLPTFEAGLHILPLALAVGIS
SPIAGVISDKVSERLPLFIAVVLYLYASFFILPKFNYYTPSWESAFKLIILGIGMGFFFAPITNLALKKLGDKTTLGVSL
MHYIRFVGGSFGTALATNDIQRYMWENFQRSTEIQNERILSEFLEKISWLFWIGEKKVKALFYQVEQLHAFSDSFMRTFF
VAGLWGLLGSLPIFYLFFELLYEKFIKKRA

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=462, Percent_Identity=28.5714285714286, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788710, Length=409, Percent_Identity=29.0953545232274, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI87081983, Length=418, Percent_Identity=25.5980861244019, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790195, Length=428, Percent_Identity=25.2336448598131, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788392, Length=408, Percent_Identity=25, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6325455, Length=152, Percent_Identity=29.6052631578947, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=520, Percent_Identity=22.3076923076923, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319770, Length=308, Percent_Identity=25, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=318, Percent_Identity=23.5849056603774, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=128, Percent_Identity=28.90625, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58155; Mature: 58023

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFYEKISPSQRIFLTVSLMVGVFMAILDTTIVDIIVPRLTGPLSTDLYGVQWVITSYM
CCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
TAAATGILLVEWLESRIGLKKVFLIGLFLFTTSSFFAGRANSLEWMITARTFQGFGESLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCEEE
VVSAEAMLFSAYPPYKRGLAMGIYGLGVSFAPALGPTLGGWITELIEWRWVFYINIPIGI
EEEHHHHHEECCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCEEEEEEECCHHH
INFVSALFFLPEHKPKHSVSLNFISYFFISVATVSLLIVLSKGQQHGWFSSDFIVKMSFL
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SLFSFLFFLLSEMLSRNKLVDPEIFKIKEFTIAFLVYCLLLGFSMYQVFYLLPLFYERLK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLPTFEAGLHILPLALAVGISSPIAGVISDKVSERLPLFIAVVLYLYASFFILPKFNYYT
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSWESAFKLIILGIGMGFFFAPITNLALKKLGDKTTLGVSLMHYIRFVGGSFGTALATND
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
IQRYMWENFQRSTEIQNERILSEFLEKISWLFWIGEKKVKALFYQVEQLHAFSDSFMRTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
FVAGLWGLLGSLPIFYLFFELLYEKFIKKRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEFYEKISPSQRIFLTVSLMVGVFMAILDTTIVDIIVPRLTGPLSTDLYGVQWVITSYM
CCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
TAAATGILLVEWLESRIGLKKVFLIGLFLFTTSSFFAGRANSLEWMITARTFQGFGESLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCEEE
VVSAEAMLFSAYPPYKRGLAMGIYGLGVSFAPALGPTLGGWITELIEWRWVFYINIPIGI
EEEHHHHHEECCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCEEEEEEECCHHH
INFVSALFFLPEHKPKHSVSLNFISYFFISVATVSLLIVLSKGQQHGWFSSDFIVKMSFL
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SLFSFLFFLLSEMLSRNKLVDPEIFKIKEFTIAFLVYCLLLGFSMYQVFYLLPLFYERLK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLPTFEAGLHILPLALAVGISSPIAGVISDKVSERLPLFIAVVLYLYASFFILPKFNYYT
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSWESAFKLIILGIGMGFFFAPITNLALKKLGDKTTLGVSLMHYIRFVGGSFGTALATND
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
IQRYMWENFQRSTEIQNERILSEFLEKISWLFWIGEKKVKALFYQVEQLHAFSDSFMRTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
FVAGLWGLLGSLPIFYLFFELLYEKFIKKRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]