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The map label for this gene is emrA [H]

Identifier: 15606343

GI number: 15606343

Start: 734303

End: 735427

Strand: Direct

Name: emrA [H]

Synonym: aq_1060

Alternate gene names: 15606343

Gene position: 734303-735427 (Clockwise)

Preceding gene: 15606342

Following gene: 15606344

Centisome position: 47.33

GC content: 42.04

Gene sequence:

>1125_bases
ATGAAAAAGTACATAGGCGTTTTTACGGTTGTGGCTTTAATTGTGGGCTTTGGGATTTACGCCTTTTTCTTCATAAAACA
CAGAATTGAGTATGCCATAACAAACGCAGTCTTCGTTAAGGCGGACGAGCTTTCTTATCTGTCCTTCAGAGTAAGCGGAA
AGGTTATTGAAGTTTACAAAGACCTCGGAGATTACGTAAAAAGGGGGGAAGCCCTTGCAAAACTGGATCCGACTTATTAC
GAACTTGAGAAAAGAACACTTGAGAAAAAAATGAGCGCCCTCCTTGAAAAGAAAAAAGCCCTTGAGATAAAAATTCAGAA
GCTGGAAAAGGGACTTCATATAAGCCTGAGTGCTAAAAAACTAAAAGTTGAGAGTTTAAAAAAGAAAAGGGAAGCCCTAC
GGGAAAAACTCCTTCAAGTGGAAGAGAAAATAAAACTCGTTAAACTTGACTGGGAACGCTACAAATCCCTCTTCCAAAAA
GGACTTATCCCGAGAAGGAAGTTTGAAGAAGTAGACACGAACCTGAAAGTTTTACTTCACGAGAGGGAATACCTTGAAAA
GAGCATACAGGAAATTAATACGGAGATAAAAAGGGCAAAGAAAGGCATTGAAAATGCAAGGAATGAGTTTAAAACAATAG
AGGAACTAAAAAAGGAACTCTCCTCCCTTGAGGAAGAAATAAAGAGCCTGAAAGAGAGGATAAAAACCGCAGAGCAGAAG
ATAAAAGACACGGTTTTGATTGCACCTTTTGACGGAGTAGTTGCAAAGAGGTTCATAAGCAGGGGAGACGTTGTTAGGGC
GGGACAGCCGGCCTTTGCCCTTGTAAACCCTGAAAGCTTTTACGTAGAGGTTTTACTCGAAGAGACGAAACTCAAAGGCG
TGAAGGTGGGAAATAAAGCTTACGTGAGACTTGACGCTTACCCTGACATCCTTTTTGAAGGAGTTGTTGAGGAGATTTCC
CCCGCTTCCGCAGCGACTTTTGCCTTAGTCCCCAGAGATGTATCCGCGGGAGAATTCACAAAAGTTGTTCAGAGGATACC
CGTAAAGATAAAGATTACGAAAGGGGATTTAAGCCTTCTGAGAGTGGGAATGGGCGGTGAGGTTGAGATAAGGAGAACGA
GATGA

Upstream 100 bases:

>100_bases
CCTTAACGAGTGCAAAGGAAGCCCTCCTGATAGCGAAAAACGAACTCTGGAAGGCTTATTACAGACTCCTTTGGGCTTCT
TCCCTTCTGGAGGTTGAAAG

Downstream 100 bases:

>100_bases
GTGAGTTTTACGAGAAAATAAGCCCTTCCCAGAGGATTTTTCTCACCGTTTCCCTTATGGTCGGAGTCTTTATGGCAATC
CTTGACACCACTATAGTTGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKKYIGVFTVVALIVGFGIYAFFFIKHRIEYAITNAVFVKADELSYLSFRVSGKVIEVYKDLGDYVKRGEALAKLDPTYY
ELEKRTLEKKMSALLEKKKALEIKIQKLEKGLHISLSAKKLKVESLKKKREALREKLLQVEEKIKLVKLDWERYKSLFQK
GLIPRRKFEEVDTNLKVLLHEREYLEKSIQEINTEIKRAKKGIENARNEFKTIEELKKELSSLEEEIKSLKERIKTAEQK
IKDTVLIAPFDGVVAKRFISRGDVVRAGQPAFALVNPESFYVEVLLEETKLKGVKVGNKAYVRLDAYPDILFEGVVEEIS
PASAATFALVPRDVSAGEFTKVVQRIPVKIKITKGDLSLLRVGMGGEVEIRRTR

Sequences:

>Translated_374_residues
MKKYIGVFTVVALIVGFGIYAFFFIKHRIEYAITNAVFVKADELSYLSFRVSGKVIEVYKDLGDYVKRGEALAKLDPTYY
ELEKRTLEKKMSALLEKKKALEIKIQKLEKGLHISLSAKKLKVESLKKKREALREKLLQVEEKIKLVKLDWERYKSLFQK
GLIPRRKFEEVDTNLKVLLHEREYLEKSIQEINTEIKRAKKGIENARNEFKTIEELKKELSSLEEEIKSLKERIKTAEQK
IKDTVLIAPFDGVVAKRFISRGDVVRAGQPAFALVNPESFYVEVLLEETKLKGVKVGNKAYVRLDAYPDILFEGVVEEIS
PASAATFALVPRDVSAGEFTKVVQRIPVKIKITKGDLSLLRVGMGGEVEIRRTR
>Mature_374_residues
MKKYIGVFTVVALIVGFGIYAFFFIKHRIEYAITNAVFVKADELSYLSFRVSGKVIEVYKDLGDYVKRGEALAKLDPTYY
ELEKRTLEKKMSALLEKKKALEIKIQKLEKGLHISLSAKKLKVESLKKKREALREKLLQVEEKIKLVKLDWERYKSLFQK
GLIPRRKFEEVDTNLKVLLHEREYLEKSIQEINTEIKRAKKGIENARNEFKTIEELKKELSSLEEEIKSLKERIKTAEQK
IKDTVLIAPFDGVVAKRFISRGDVVRAGQPAFALVNPESFYVEVLLEETKLKGVKVGNKAYVRLDAYPDILFEGVVEEIS
PASAATFALVPRDVSAGEFTKVVQRIPVKIKITKGDLSLLRVGMGGEVEIRRTR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=375, Percent_Identity=23.2, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788711, Length=377, Percent_Identity=24.1379310344828, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790519, Length=352, Percent_Identity=24.7159090909091, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081951, Length=196, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42871; Mature: 42871

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYIGVFTVVALIVGFGIYAFFFIKHRIEYAITNAVFVKADELSYLSFRVSGKVIEVYK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECCHHHHHHH
DLGDYVKRGEALAKLDPTYYELEKRTLEKKMSALLEKKKALEIKIQKLEKGLHISLSAKK
HHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
LKVESLKKKREALREKLLQVEEKIKLVKLDWERYKSLFQKGLIPRRKFEEVDTNLKVLLH
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHCCCCCHHHHHHCCCHHHHHH
EREYLEKSIQEINTEIKRAKKGIENARNEFKTIEELKKELSSLEEEIKSLKERIKTAEQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKDTVLIAPFDGVVAKRFISRGDVVRAGQPAFALVNPESFYVEVLLEETKLKGVKVGNKA
HHCEEEEECCHHHHHHHHHCCCCEEECCCCEEEEECCCHHHHHHHHHHHHCCCEEECCEE
YVRLDAYPDILFEGVVEEISPASAATFALVPRDVSAGEFTKVVQRIPVKIKITKGDLSLL
EEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCEEEE
RVGMGGEVEIRRTR
EECCCCCEEEEECC
>Mature Secondary Structure
MKKYIGVFTVVALIVGFGIYAFFFIKHRIEYAITNAVFVKADELSYLSFRVSGKVIEVYK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECCHHHHHHH
DLGDYVKRGEALAKLDPTYYELEKRTLEKKMSALLEKKKALEIKIQKLEKGLHISLSAKK
HHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
LKVESLKKKREALREKLLQVEEKIKLVKLDWERYKSLFQKGLIPRRKFEEVDTNLKVLLH
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHCCCCCHHHHHHCCCHHHHHH
EREYLEKSIQEINTEIKRAKKGIENARNEFKTIEELKKELSSLEEEIKSLKERIKTAEQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKDTVLIAPFDGVVAKRFISRGDVVRAGQPAFALVNPESFYVEVLLEETKLKGVKVGNKA
HHCEEEEECCHHHHHHHHHCCCCEEECCCCEEEEECCCHHHHHHHHHHHHCCCEEECCEE
YVRLDAYPDILFEGVVEEISPASAATFALVPRDVSAGEFTKVVQRIPVKIKITKGDLSLL
EEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCEEEE
RVGMGGEVEIRRTR
EECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]